##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937181_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 291352 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.915473379280048 33.0 31.0 34.0 31.0 34.0 2 31.970681512397373 33.0 31.0 34.0 30.0 34.0 3 31.96185027046322 33.0 31.0 34.0 30.0 34.0 4 35.63925423542656 37.0 35.0 37.0 35.0 37.0 5 35.651459403058844 37.0 35.0 37.0 35.0 37.0 6 35.75419080699635 37.0 35.0 37.0 35.0 37.0 7 35.72033485268678 37.0 35.0 37.0 35.0 37.0 8 35.73561533814767 37.0 35.0 37.0 35.0 37.0 9 37.37216494137675 39.0 37.0 39.0 35.0 39.0 10-11 37.28861480271287 39.0 37.0 39.0 35.0 39.0 12-13 37.17814362008842 39.0 37.0 39.0 34.0 39.0 14-15 38.24096110546692 40.0 38.0 41.0 33.5 41.0 16-17 38.239366814025644 40.0 38.0 41.0 34.0 41.0 18-19 38.34722775199758 40.0 38.0 41.0 34.0 41.0 20-21 38.37662518191054 40.0 38.0 41.0 34.0 41.0 22-23 38.258383330129874 40.0 38.0 41.0 34.0 41.0 24-25 38.116487959581534 40.0 38.0 41.0 34.0 41.0 26-27 37.92114349652654 40.0 37.5 41.0 33.5 41.0 28-29 37.70715148686125 40.0 37.0 41.0 33.0 41.0 30-31 37.42109201241111 39.0 36.5 41.0 33.0 41.0 32-33 37.039429967873915 39.0 35.0 41.0 33.0 41.0 34-35 36.75378923089596 39.0 35.0 41.0 32.5 41.0 36-37 36.61953238694088 39.0 35.0 41.0 31.5 41.0 38-39 36.49874207144622 39.0 35.0 41.0 31.0 41.0 40-41 36.19513852659326 39.0 35.0 41.0 30.5 41.0 42-43 35.77301683187348 39.0 35.0 41.0 30.0 41.0 44-45 35.20528089733381 39.0 35.0 41.0 26.5 41.0 46-47 34.43511800159257 38.5 35.0 41.0 21.0 41.0 48-49 33.79893393558308 38.0 34.5 40.5 8.5 41.0 50-51 32.730961174112416 37.5 33.0 39.5 2.0 40.5 52-53 32.75583658255306 38.0 33.0 39.5 2.0 40.5 54-55 33.011704055575386 38.0 34.0 40.0 2.0 41.0 56-57 32.75067787418655 38.0 33.5 40.0 2.0 41.0 58-59 32.48309604876576 38.0 33.0 40.0 2.0 41.0 60-61 32.17245462533293 37.0 33.0 40.0 2.0 41.0 62-63 31.811648795958153 37.0 33.0 40.0 2.0 41.0 64-65 31.404519275652817 36.0 32.0 40.0 2.0 41.0 66-67 30.969313751064007 35.5 31.0 39.0 2.0 41.0 68-69 30.481266646530656 35.0 31.0 39.0 2.0 41.0 70-71 30.020569620253163 35.0 30.0 38.5 2.0 40.0 72-73 29.615496032290835 35.0 29.5 38.0 2.0 40.0 74-75 29.172252121145554 35.0 29.0 37.0 2.0 39.5 76-77 28.739250116697328 35.0 29.0 37.0 2.0 39.0 78-79 28.288002141739202 35.0 28.0 36.0 2.0 39.0 80-81 27.85362894368324 34.0 27.0 36.0 2.0 37.5 82-83 27.433056920838027 34.0 26.0 35.0 2.0 37.0 84-85 26.98540253713721 34.0 25.0 35.0 2.0 37.0 86-87 26.546129767429086 34.0 24.0 35.0 2.0 36.0 88-89 26.15393064059969 34.0 22.5 35.0 2.0 36.0 90-91 25.712159175155826 33.5 19.5 35.0 2.0 35.5 92-93 25.30304580026909 33.0 16.5 35.0 2.0 35.0 94-95 24.805837955462806 33.0 4.5 35.0 2.0 35.0 96-97 24.242371770229823 32.5 2.0 35.0 2.0 35.0 98-99 23.626753892199126 32.0 2.0 35.0 2.0 35.0 100-101 22.11650855322771 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15681514436066024 1101 2 -0.019596444868081875 1101 3 0.1051749807403688 1101 4 0.12998511977355776 1101 5 0.14600050895998606 1101 6 -0.047063352398978964 1101 7 0.18699244946927251 1101 8 0.036160875626656264 1101 9 0.20279748114417373 1101 10-11 0.02860132689772854 1101 12-13 0.048952746101228684 1101 14-15 0.22540210564858398 1101 16-17 0.05438024870652214 1101 18-19 0.17207789941026164 1101 20-21 0.1696523446068241 1101 22-23 0.011601263083711899 1101 24-25 0.14041981872441767 1101 26-27 0.19737427751285708 1101 28-29 0.16083327223827837 1101 30-31 0.2875923051007234 1101 32-33 0.3515517847731431 1101 34-35 0.2803226956410967 1101 36-37 0.4114569382663973 1101 38-39 0.5749911479714314 1101 40-41 0.6227142664662537 1101 42-43 0.8521816424008364 1101 44-45 0.5470858535880936 1101 46-47 1.0453619355144355 1101 48-49 1.12657795949211 1101 50-51 1.0423364333655414 1101 52-53 1.349072713476808 1101 54-55 1.3086166869291702 1101 56-57 1.535359047549811 1101 58-59 1.486750715843705 1101 60-61 1.4224925966617086 1101 62-63 1.5676540598431252 1101 64-65 1.6259099899553426 1101 66-67 1.8544838219715523 1101 68-69 1.5839184898182914 1101 70-71 1.5718309050702075 1101 72-73 1.5813680814658717 1101 74-75 1.7165868231528698 1101 76-77 1.8142993264990679 1101 78-79 1.842822758406534 1101 80-81 1.9599698974578779 1101 82-83 1.8980685433520001 1101 84-85 1.839192499105259 1101 86-87 1.91487114649032 1101 88-89 1.6386809508421152 1101 90-91 1.5415601410845703 1101 92-93 1.5503103708272619 1101 94-95 1.38431031178078 1101 96-97 1.3459868739394736 1101 98-99 1.853869796550459 1101 100-101 2.1332618284886706 1102 1 0.05055157534613386 1102 2 -0.19696164999800914 1102 3 -0.08165775947315268 1102 4 -0.22678204153265114 1102 5 0.11279139505860059 1102 6 0.042827871666013095 1102 7 0.09747612188808574 1102 8 0.15502544287301845 1102 9 0.11316924416482976 1102 10-11 -0.07629694745935467 1102 12-13 0.006799273552601903 1102 14-15 0.054989271878795876 1102 16-17 0.4091302431110577 1102 18-19 0.21299757084427284 1102 20-21 0.2494325545488536 1102 22-23 0.2779847989730584 1102 24-25 0.1894501237894417 1102 26-27 0.14259182938286585 1102 28-29 0.12274490857750209 1102 30-31 0.003110221795374457 1102 32-33 0.021564542444529877 1102 34-35 0.08044971280799729 1102 36-37 0.11717159168298963 1102 38-39 0.45361421473657515 1102 40-41 0.38459624681495796 1102 42-43 0.1217406074627192 1102 44-45 0.1739519428022689 1102 46-47 0.7291815151827308 1102 48-49 1.0119939267250402 1102 50-51 1.0543247276424879 1102 52-53 1.2079381759983505 1102 54-55 1.445926720725808 1102 56-57 1.4360730295100126 1102 58-59 1.0577572289706794 1102 60-61 1.1731388734360344 1102 62-63 1.4897175572356396 1102 64-65 1.6310354403906722 1102 66-67 1.700751083996213 1102 68-69 1.7008526199465415 1102 70-71 1.7066621456977913 1102 72-73 1.9125975181139658 1102 74-75 1.920564083163164 1102 76-77 1.9855570762248895 1102 78-79 2.013428635888129 1102 80-81 2.0083539257485725 1102 82-83 1.917238623255308 1102 84-85 1.9047909905665747 1102 86-87 1.5932015701793034 1102 88-89 1.3955997493828143 1102 90-91 1.6930407024221132 1102 92-93 1.4018317790951293 1102 94-95 1.4254398133363217 1102 96-97 1.5162150572023059 1102 98-99 1.7919838993728163 1102 100-101 1.8525986533952263 1103 1 -0.09543190943429636 1103 2 -0.2672791706420341 1103 3 -0.20356288333730177 1103 4 -0.22230772995472137 1103 5 -0.300897579823058 1103 6 -0.29301499189339353 1103 7 -0.11440585302813133 1103 8 -0.0616867155072498 1103 9 0.04194995107150845 1103 10-11 0.028528053404414777 1103 12-13 0.02431300744335374 1103 14-15 0.018818176968110834 1103 16-17 -0.01358854899757489 1103 18-19 -0.07390427070637173 1103 20-21 0.0776086247558041 1103 22-23 0.01292934515002031 1103 24-25 -0.12819317510839312 1103 26-27 0.05311250795799083 1103 28-29 -0.2650414300881465 1103 30-31 0.11373109730930508 1103 32-33 0.09808045302739998 1103 34-35 0.22615679223998342 1103 36-37 0.31032883177559256 1103 38-39 0.18024241499225013 1103 40-41 0.22361949941490167 1103 42-43 0.42217767341994517 1103 44-45 0.11733376224047021 1103 46-47 0.4718274053807079 1103 48-49 0.8279701558650672 1103 50-51 0.8304951315398057 1103 52-53 1.226232754998449 1103 54-55 1.3909928852560896 1103 56-57 1.3801596825867506 1103 58-59 1.1801011463254838 1103 60-61 1.4382876140641692 1103 62-63 1.3647063282683582 1103 64-65 1.2844127682419746 1103 66-67 1.8455062925095014 1103 68-69 1.7280474511665211 1103 70-71 1.8190571980420565 1103 72-73 1.9423957422096017 1103 74-75 2.213723050789188 1103 76-77 2.1350647291979747 1103 78-79 1.7993837891203377 1103 80-81 1.80080460466921 1103 82-83 2.0815270522364067 1103 84-85 2.2504842497311373 1103 86-87 2.093021906702816 1103 88-89 2.3368964359736992 1103 90-91 2.160675363652807 1103 92-93 1.7263575711692063 1103 94-95 1.8437274822027163 1103 96-97 2.0788485091596023 1103 98-99 1.715340151316287 1103 100-101 1.7762123078194385 1104 1 -0.0937310734710124 1104 2 0.1486932690698346 1104 3 0.18954222639793628 1104 4 -0.018906058028150596 1104 5 0.037033953097513006 1104 6 0.01936063748818384 1104 7 0.07456521348756695 1104 8 0.2757437212654992 1104 9 0.06788167672137035 1104 10-11 0.10564145049902152 1104 12-13 0.22310844939570273 1104 14-15 0.10034748386541281 1104 16-17 -0.17826367969211532 1104 18-19 -0.12507630568673278 1104 20-21 0.03401085077908306 1104 22-23 0.1541758218177378 1104 24-25 0.21406753943544032 1104 26-27 0.19324838210576445 1104 28-29 0.1877423035599719 1104 30-31 0.11264458595032778 1104 32-33 -0.10118476656353437 1104 34-35 0.010586674916034156 1104 36-37 -0.076789624462819 1104 38-39 0.03723969269106675 1104 40-41 -0.10730391478443124 1104 42-43 0.09812987655661942 1104 44-45 0.32636312936567435 1104 46-47 0.44469593991539114 1104 48-49 0.5002845936978346 1104 50-51 0.6406302829527561 1104 52-53 0.5139173535192612 1104 54-55 0.5643071062684015 1104 56-57 0.7591835087449326 1104 58-59 0.4579806423383985 1104 60-61 0.6699819454139977 1104 62-63 0.7198244954128334 1104 64-65 0.6802040424234725 1104 66-67 0.8110029317925473 1104 68-69 1.0894196836944126 1104 70-71 0.8892010222729425 1104 72-73 0.8826111171885209 1104 74-75 0.6834235976585425 1104 76-77 0.6669590171837143 1104 78-79 0.6589034113709396 1104 80-81 1.083895018796266 1104 82-83 0.9272833942345322 1104 84-85 1.025275258909872 1104 86-87 0.8580391318828973 1104 88-89 0.6616669676656244 1104 90-91 0.825386631088783 1104 92-93 1.18103005183605 1104 94-95 0.8545082767292023 1104 96-97 0.6364167473730014 1104 98-99 0.6174433096674896 1104 100-101 0.3051409738918558 1105 1 0.1321394730756431 1105 2 -0.1187748894827152 1105 3 0.14576538664443106 1105 4 0.19667267344154737 1105 5 0.11652082737072078 1105 6 -0.06434734599363878 1105 7 -0.06360515084634955 1105 8 -0.04019852004277169 1105 9 0.02764837116250618 1105 10-11 0.08548359383360093 1105 12-13 -0.022077563258470434 1105 14-15 -0.2999436902622108 1105 16-17 -0.08260656927486565 1105 18-19 -0.0020094546708264716 1105 20-21 -0.24820372074628239 1105 22-23 -0.3298141793725975 1105 24-25 -0.1589924442661328 1105 26-27 -0.10800238474433144 1105 28-29 0.16297418987387147 1105 30-31 0.33036134128165173 1105 32-33 0.3567735351038479 1105 34-35 0.04852629748842929 1105 36-37 0.016834760912544766 1105 38-39 0.11527985274527452 1105 40-41 0.16344682001820132 1105 42-43 0.5064466520320607 1105 44-45 -0.3684900474539319 1105 46-47 -0.15732030357210647 1105 48-49 0.02736470320859752 1105 50-51 -0.44875468037578514 1105 52-53 -0.3426061372739646 1105 54-55 0.28280673543342516 1105 56-57 0.12713421058445817 1105 58-59 0.6202186542966786 1105 60-61 0.5904676018834643 1105 62-63 0.4939722617043927 1105 64-65 0.5474909038776943 1105 66-67 0.387784084219458 1105 68-69 0.5452079140332877 1105 70-71 0.3367622100436307 1105 72-73 0.3370075718230403 1105 74-75 0.456033085540021 1105 76-77 0.6969462897611507 1105 78-79 0.6998280265724226 1105 80-81 0.669704400463285 1105 82-83 0.7936683484066513 1105 84-85 1.0041330871903895 1105 86-87 0.5413506424173562 1105 88-89 0.679059585760541 1105 90-91 0.27297070990724137 1105 92-93 0.5036656355119824 1105 94-95 0.45344338518698635 1105 96-97 0.16519537262823647 1105 98-99 -0.29765379415714577 1105 100-101 0.3578086965988625 1106 1 -0.1305226472164449 1106 2 0.1643459164088874 1106 3 -0.1395507576562558 1106 4 -0.08405038695361355 1106 5 0.07417103678420744 1106 6 -0.052934700221605624 1106 7 0.04695582818116151 1106 8 0.038210871646086275 1106 9 0.17023637932106794 1106 10-11 -0.2102331608023036 1106 12-13 -0.612748153508683 1106 14-15 -0.3451038254078611 1106 16-17 -0.38663758426855566 1106 18-19 -0.19702089845615234 1106 20-21 -0.3336117267250813 1106 22-23 0.04195314589559018 1106 24-25 -0.16089974182012412 1106 26-27 0.11892709974038951 1106 28-29 -0.03729983818286087 1106 30-31 -0.33649216269642324 1106 32-33 -0.001206753559792162 1106 34-35 0.054435027461813945 1106 36-37 -0.4612741341004494 1106 38-39 -0.4309001027251753 1106 40-41 -0.18354120682408848 1106 42-43 -0.36486914973355056 1106 44-45 -0.4286127372884252 1106 46-47 -0.55716771976779 1106 48-49 0.01042478697399929 1106 50-51 0.2067640267100188 1106 52-53 0.13760467749241911 1106 54-55 -0.10071577067770932 1106 56-57 -0.04906894385214855 1106 58-59 0.10366019757287148 1106 60-61 0.3466526953799658 1106 62-63 0.2786344754151351 1106 64-65 0.35332022799372 1106 66-67 0.33859137710251375 1106 68-69 0.2658705636977601 1106 70-71 0.3078491361027673 1106 72-73 0.23165468285111146 1106 74-75 -0.4005614930639929 1106 76-77 -0.17452520976397068 1106 78-79 -0.2272353579854709 1106 80-81 -0.3266911964040773 1106 82-83 -0.3226145232828266 1106 84-85 -0.3084768743522659 1106 86-87 -0.2777986250246869 1106 88-89 0.0388381835465168 1106 90-91 0.19244382868906484 1106 92-93 -0.10122016018141622 1106 94-95 -0.011727026010795782 1106 96-97 -0.1414046553973627 1106 98-99 -0.2754590785391038 1106 100-101 -0.4247749520224353 1107 1 -0.3723852150283804 1107 2 -0.19756889170845326 1107 3 -0.1289520033866154 1107 4 -0.17542069034923458 1107 5 -0.2747841571076961 1107 6 -0.1868507138844535 1107 7 -0.40547535526268064 1107 8 -0.19250040020443038 1107 9 -0.2755643038213904 1107 10-11 -0.2534067593556415 1107 12-13 0.1988853606564831 1107 14-15 0.08756143478903766 1107 16-17 0.1717057952773544 1107 18-19 -0.3297564821411001 1107 20-21 -0.6003386706672131 1107 22-23 -0.539948602145401 1107 24-25 -0.4633912025394338 1107 26-27 -0.3603525868177826 1107 28-29 -0.46469083677639134 1107 30-31 -0.37095067183847874 1107 32-33 -0.43640868243260655 1107 34-35 -0.2972828748889995 1107 36-37 -0.4271923579300676 1107 38-39 -0.6053023530767803 1107 40-41 -1.0424923822309609 1107 42-43 -1.123634470207726 1107 44-45 -0.8818225022070507 1107 46-47 -0.564476339418178 1107 48-49 -0.5331255445510195 1107 50-51 -0.26857252141134325 1107 52-53 -0.4770728390838386 1107 54-55 -0.304553423882453 1107 56-57 0.1666483557259042 1107 58-59 0.5269573645409196 1107 60-61 0.44487953885997555 1107 62-63 0.6037801454975167 1107 64-65 0.21691777671784962 1107 66-67 0.12937900016861548 1107 68-69 0.22834795971946065 1107 70-71 0.6837281797033299 1107 72-73 0.8261644276773161 1107 74-75 0.8311795155757515 1107 76-77 0.7291316116143705 1107 78-79 0.8147829261214206 1107 80-81 1.034168759392589 1107 82-83 1.1330565637173784 1107 84-85 0.9150011244421883 1107 86-87 0.8626474268137727 1107 88-89 0.5864572311655643 1107 90-91 0.7277311503687898 1107 92-93 0.652848555912616 1107 94-95 0.6149557145959967 1107 96-97 0.312181842492933 1107 98-99 0.6846574575268001 1107 100-101 0.7852160063862463 1108 1 0.07721425092622525 1108 2 -0.087162423825351 1108 3 -0.27800258935308975 1108 4 0.06753287737068803 1108 5 -0.1396780897586396 1108 6 0.1197061767845824 1108 7 0.01994372202555894 1108 8 0.1460621276104348 1108 9 0.2882615380418372 1108 10-11 0.21927904393763242 1108 12-13 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60-61 -2.15752229906818 2316 62-63 -2.220321775972824 2316 64-65 -2.4759602516864945 2316 66-67 -1.8138958536325553 2316 68-69 -1.2338074287517173 2316 70-71 -1.3651022296533952 2316 72-73 -1.3875991479283662 2316 74-75 -1.6143300917759653 2316 76-77 -1.3825642948183798 2316 78-79 -1.26957379902478 2316 80-81 -1.469507096914846 2316 82-83 -1.902427980977695 2316 84-85 -1.6873798279387628 2316 86-87 -1.525322497998257 2316 88-89 -1.174319711190325 2316 90-91 -1.6138519741090747 2316 92-93 -1.1960236245385119 2316 94-95 -0.8808254048693342 2316 96-97 -0.36588833294566214 2316 98-99 -0.16830829017579063 2316 100-101 -0.8688566753180176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1283.0 3 203.0 4 170.0 5 164.0 6 184.0 7 241.0 8 285.0 9 412.0 10 558.0 11 832.0 12 1390.0 13 1983.0 14 2421.0 15 3472.0 16 5214.0 17 7681.0 18 5887.0 19 3197.0 20 2946.0 21 2872.0 22 2993.0 23 2997.0 24 3134.0 25 3241.0 26 3465.0 27 3779.0 28 4240.0 29 4932.0 30 5976.0 31 7152.0 32 9205.0 33 12010.0 34 17165.0 35 26219.0 36 35401.0 37 51051.0 38 48208.0 39 8784.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.733030271722285 19.767193580604054 16.037813823742123 18.461962323931537 2 14.9770711688884 21.878098490690757 41.838575520546435 21.306254819874408 3 17.806851437424687 29.711482182819765 31.783439490445858 20.698226889309694 4 11.114630493536005 17.993837255340758 39.03324094954468 31.858291301578557 5 11.145611015490534 39.48433734939759 33.503614457831326 15.86643717728055 6 26.644099748530227 39.012215720472945 18.181849455263492 16.16183507573334 7 24.224805534817953 37.9118516250443 21.034234816922343 16.82910802321541 8 26.484227183597646 33.14733908975197 20.052289380439646 20.31614434621074 9 28.4135354640303 15.53337942848887 20.7015407477735 35.35154435970733 10-11 23.833786017645227 28.597206143662905 26.19141407799472 21.37759376069714 12-13 26.49534277678871 27.147268020400617 27.94626347068487 18.41112573212581 14-15 22.950633590622033 26.614589782749277 29.631196598836006 20.803580027792684 16-17 18.511058438229963 30.7052700020297 29.570012694240805 21.21365886549953 18-19 18.261713039559993 26.35997151928124 36.66686158301889 18.71145385813988 20-21 20.24945693582813 25.109257803698853 36.80814140479479 17.833143855678223 22-23 19.40922029117614 25.77742997927541 36.836447754263155 17.976901975285287 24-25 18.69126893496416 25.98544363881513 37.17375452306592 18.149532903154793 26-27 18.18421048105385 26.415392897623196 37.11746079795532 18.282935823367634 28-29 17.96519447817517 26.81036528942109 37.118379365740054 18.106060866663682 30-31 18.26535386478416 26.65046305852564 36.980521267665715 18.103661809024484 32-33 17.836009852894634 27.01650635071351 36.62032641153725 18.527157384854615 34-35 18.212918339336024 27.389810218676793 36.26519162168949 18.13207982029769 36-37 18.112459883666933 27.278934496434676 36.008042185355514 18.60056343454287 38-39 18.121188565610844 27.611208755679534 35.92800365966492 18.3395990190447 40-41 18.137792743369527 27.669988064978796 35.82432474264036 18.367894449011317 42-43 17.879045322748738 27.963417108933502 35.297536949201515 18.860000619116246 44-45 18.006484515424376 28.297255691151303 34.727418148043036 18.96884164538129 46-47 18.399111190605588 28.562976823538317 33.760482378597025 19.27742960725907 48-49 18.49238019883725 28.90175100622657 32.98032268051877 19.625546114417418 50-51 18.76780332741609 29.307201144917368 32.38795084561505 19.537044682051494 52-53 18.78927585346763 29.662397536577185 31.873457135238816 19.674869474716374 54-55 18.431174889990608 29.334404469873427 32.060297876878934 20.174122763257035 56-57 18.81125290841148 29.233383146776543 31.629931401901622 20.325432542910356 58-59 18.813156279391258 28.821357891171317 32.13140460768957 20.234081221747854 60-61 18.88325815820653 28.673277950788524 32.57831482758443 19.865149063420525 62-63 19.048118557853357 28.394282589480706 32.7083068224418 19.849292030224134 64-65 18.801274522784762 28.526302938177388 32.82979480733418 19.84262773170367 66-67 18.750021497387635 28.422298122332172 32.62254370848854 20.205136671791642 68-69 18.834645940090105 28.209925370567802 32.65966227602573 20.295766413316365 70-71 18.82134443087823 28.316623961754743 32.476655603514985 20.38537600385204 72-73 18.742615380646495 28.216770287817422 32.30331329704443 20.737301034491654 74-75 18.4536144526514 28.32334454027189 32.28836513409632 20.93467587298039 76-77 18.17587671774762 28.05376399566586 32.65982147464011 21.110537811946408 78-79 18.26110436538376 27.8249787595582 32.70701261355467 21.206904261503375 80-81 18.16566928050626 28.126633649745497 32.26698995735314 21.440707112395103 82-83 18.01537929694151 27.980276249460385 32.13313359624787 21.871210857350228 84-85 18.119007332205303 27.692315628740044 32.00273754006576 22.185939498988898 86-87 18.22355896964976 27.710739779643035 31.776183081661397 22.289518169045813 88-89 18.094188218217838 27.279637610624036 31.804861438344624 22.821312732813507 90-91 18.208630779893273 27.55465822379905 31.807792913563222 22.42891808274446 92-93 18.38209876224725 27.586734702652134 31.535435982368433 22.495730552732187 94-95 18.346449651225182 27.7480635086609 31.133655737930464 22.771831102183455 96-97 18.504238964076283 27.801757493422297 30.745301026637545 22.94870251586387 98-99 18.87901546250802 27.817724357706574 30.327049844274264 22.976210335511144 100-101 19.373642643060908 27.45235601914185 30.152711790670843 23.0212895471264 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 877.0 1 586.5 2 401.5 3 509.0 4 432.0 5 424.5 6 784.5 7 1700.0 8 2790.5 9 3175.0 10 2500.0 11 1735.5 12 1592.0 13 1657.0 14 1676.5 15 1643.0 16 1593.0 17 1553.5 18 1618.0 19 1712.5 20 1808.0 21 1898.0 22 2057.5 23 2303.5 24 2595.0 25 2949.0 26 3274.5 27 3757.0 28 4137.5 29 4505.5 30 5091.0 31 5543.0 32 5955.0 33 6399.0 34 6883.5 35 7350.0 36 7849.0 37 8368.0 38 8546.0 39 8365.5 40 8199.0 41 8205.5 42 8101.0 43 8193.5 44 8558.5 45 8688.5 46 8661.0 47 8890.0 48 9603.0 49 9456.0 50 8588.5 51 7976.5 52 7317.5 53 6948.5 54 6635.0 55 6114.5 56 5540.0 57 4871.5 58 4313.5 59 3777.5 60 3260.5 61 2844.5 62 2330.5 63 1682.0 64 1121.0 65 830.0 66 606.5 67 443.0 68 328.0 69 239.0 70 182.0 71 144.5 72 120.5 73 101.0 74 92.5 75 70.5 76 49.0 77 28.5 78 16.5 79 15.5 80 14.0 81 11.0 82 8.5 83 5.0 84 3.0 85 1.5 86 0.0 87 0.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3370493423762322 2 0.304785963370768 3 0.3095911474779648 4 0.3078750102968231 5 0.2924297756665477 6 0.2268733353469343 7 0.23442433894395784 8 0.2272165627831626 9 0.22275460611219416 10-11 0.23287981548093029 12-13 0.2315069057360169 14-15 0.21554682995139898 16-17 0.22961915483676104 18-19 0.21589005738762734 20-21 0.21983717290425328 22-23 0.2177778082868832 24-25 0.21280101046157224 26-27 0.22189653752162333 28-29 0.22344106098465089 30-31 0.23202174689035943 32-33 0.23202174689035943 34-35 0.22275460611219416 36-37 0.2179494220049974 38-39 0.21108487328043052 40-41 0.2097119635355172 42-43 0.21108487328043052 44-45 0.22635849419259177 46-47 0.21486037507894232 48-49 0.2272165627831626 50-51 0.23202174689035943 52-53 0.2400875916417255 54-55 0.23854306817869794 56-57 0.20610807545511955 58-59 0.1906628408248442 60-61 0.2013028913479228 62-63 0.2028474148109503 64-65 0.19787061698563935 66-67 0.21245778302534393 68-69 0.19975836788489526 70-71 0.2049067794283204 72-73 0.21606167110574148 74-75 0.20919712238117466 76-77 0.2186358768774541 78-79 0.21726296713254067 80-81 0.20319064224717867 82-83 0.21846426315933992 84-85 0.1990719130124386 86-87 0.20473516571020622 88-89 0.21108487328043052 90-91 0.21057003212608802 92-93 0.21400230648837149 94-95 0.21280101046157224 96-97 0.2049067794283204 98-99 0.21159971443477305 100-101 0.19752738954941104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 291352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.526650533280836 #Duplication Level Percentage of deduplicated Percentage of total 1 81.48640684161305 50.950720837982836 2 11.055111635469443 13.82478203674809 3 3.3956118898447682 6.369487139489313 4 1.4193197284826629 3.54981234631306 5 0.749634557883763 2.343606901423427 6 0.45695764562410474 1.714321860986951 7 0.29012696225108214 1.2698467025269045 8 0.20643599676101657 1.032620114157246 9 0.15555714158542264 0.8753820326881028 >10 0.7225508091675038 7.9322267034650356 >50 0.03715352464533532 1.574573653871381 >100 0.020762263772393263 2.2837979770297636 >500 0.002185501449725607 1.0088340203693182 >1k 0.001639126087294205 3.1409481826195433 >5k 5.463753624314017E-4 2.1290394903290184 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6232 2.1389933825750296 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4607 1.5812487987039732 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3389 1.1631977813778522 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1198 0.4111864686015541 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 954 0.3274389741618386 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 903 0.3099343749141931 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.1946099563414701 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 0.1815673137647931 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 463 0.15891430297372253 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 441 0.15136329937669896 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 398 0.13660451961888026 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.864548724566847E-4 0.0 24-25 0.0 0.0 0.0 0.0027458194898267387 0.0 26-27 0.0 0.0 0.0 0.0036038880803975944 0.0 28-29 0.0 0.0 0.0 0.0063497075702243335 0.0 30-31 0.0 0.0 0.0 0.014415552321590377 0.0 32-33 0.0 0.0 0.0 0.0202504187374722 0.0 34-35 0.0 0.0 0.0 0.026771740025810703 0.0 36-37 0.0 0.0 0.0 0.03363628875037755 0.0 38-39 0.0 0.0 0.0 0.04153051978362943 0.0 40-41 0.0 0.0 0.0 0.06057964249430243 0.0 42-43 0.0 0.0 0.0 0.09850627419753426 0.0 44-45 0.0 0.0 0.0 0.15273620912161234 0.0 46-47 0.0 0.0 0.0 0.21177132815288724 0.0 48-49 0.0 0.0 0.0 0.33275899942337794 0.0 50-51 0.0 0.0 0.0 0.4841222988000769 0.0 52-53 0.0 0.0 0.0 0.6349707570224334 0.0 54-55 0.0 0.0 0.0 0.8402207638869821 0.0 56-57 0.0 0.0 0.0 1.0633185974354045 0.0 58-59 0.0 0.0 0.0 1.362441308108405 0.0 60-61 0.0 0.0 0.0 1.6936557840687554 0.0 62-63 0.0 0.0 0.0 2.119429418710014 0.0 64-65 0.0 0.0 0.0 2.4935473241989072 0.0 66-67 0.0 0.0 0.0 2.9254990526922757 0.0 68-69 0.0 0.0 0.0 3.5036656690189187 0.0 70-71 0.0 0.0 0.0 4.122676350256734 0.0 72-73 0.0 0.0 0.0 4.833328756967517 0.0 74-75 0.0 0.0 0.0 5.599584008347291 0.0 76-77 0.0 0.0 0.0 6.358803097284385 0.0 78-79 0.0 0.0 0.0 7.135183558032894 0.0 80-81 0.0 0.0 0.0 8.045937560064802 0.0 82-83 0.0 0.0 0.0 9.153360883055548 0.0 84-85 0.0 0.0 0.0 10.247741563469617 0.0 86-87 0.0 0.0 0.0 11.329251215025124 0.0 88-89 0.0 0.0 0.0 12.682940223509707 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2020 0.0 53.894596 1 GTATCAA 3590 0.0 39.46235 1 TATATAG 60 0.003936453 31.682562 3 TATCAAC 4640 0.0 30.316935 2 ATCAACG 4635 0.0 30.144573 3 TCAACGC 4675 0.0 29.886652 4 GTACTCA 80 4.4813342E-4 29.692177 6 CAACGCA 4740 0.0 29.376553 5 TACATGG 2565 0.0 29.088669 2 GTACATG 2630 0.0 28.921799 1 AACGCAG 4835 0.0 28.887695 6 ACATGGG 2630 0.0 27.64695 3 CTATACT 105 6.8648864E-5 27.15648 4 CATGGGT 235 5.456968E-12 26.289785 4 ACGCAGA 5325 0.0 26.051054 7 CGCAGAG 5340 0.0 25.977877 8 GACTCCC 130 9.975287E-6 25.580954 9 CATGGGA 1920 0.0 25.247042 4 ATGGGAC 320 0.0 25.247042 5 GAGTACT 3590 0.0 25.209404 12-13 >>END_MODULE