FastQCFastQC Report
Thu 26 May 2016
SRR937181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291352
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54201.8602927043576154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46761.6049314918037287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33681.1559900052170569No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19210.6593399049946457No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.31336664927647656No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.3071885554243664No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.23511079381641453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.1702408083692578No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.16406271451714763No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.13557483731019523No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2980.10228177599604601No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGG205.386844E-447.49845510-11
ATTGGCG405.3823827E-447.4984557
GGTATCA27300.042.8081551
CAGTGCG500.001614094137.9987649
CCTACCT651.3260054E-436.5372733
GTATCAA43550.034.2527541
GTGCATA902.4045412E-531.6710721
ATACGTC600.003946782631.6656365
GTAGGAC953.4827703E-529.9990253
TGTAGGA1751.5097612E-1029.8561732
GCTTATA1153.847388E-628.9170651
ATATTGG856.39683E-427.9402685
TCGTTCA350.00838956427.14197356-57
GCCTTCG350.00838956427.14197374-75
GACGTGG1056.8899746E-527.1419737
ACCTCGG700.008382827.1419736
CCATATA700.008382827.1419732
TGTAAGG700.008382827.1419732
GATAGTC700.008382827.1419737
TATCAAC55950.026.4870742
Produced by FastQC (version 0.11.5)