##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937181_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 291352 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.037284796397486 31.0 31.0 34.0 31.0 34.0 2 32.223897553474835 33.0 31.0 34.0 30.0 34.0 3 32.23493231554958 34.0 31.0 34.0 30.0 34.0 4 35.95545937560065 37.0 35.0 37.0 35.0 37.0 5 35.870019769900324 37.0 35.0 37.0 35.0 37.0 6 35.98757516680853 37.0 35.0 37.0 35.0 37.0 7 35.934532798813805 37.0 35.0 37.0 35.0 37.0 8 35.932514621488785 37.0 35.0 37.0 35.0 37.0 9 37.569095115187125 39.0 37.0 39.0 35.0 39.0 10-11 37.503947115516624 39.0 37.0 39.0 35.0 39.0 12-13 37.404685740959394 39.0 37.0 39.0 34.0 39.0 14-15 38.3927362777671 40.0 38.0 41.0 34.0 41.0 16-17 38.404428320382216 40.0 38.0 41.0 34.0 41.0 18-19 38.57283114583048 40.0 38.0 41.0 34.0 41.0 20-21 38.56029304758505 40.0 38.0 41.0 34.0 41.0 22-23 38.431646256075126 40.0 38.0 41.0 34.0 41.0 24-25 38.27183612949285 40.0 38.0 41.0 34.0 41.0 26-27 38.109498132842745 40.0 37.0 41.0 33.5 41.0 28-29 37.93672258985694 40.0 37.0 41.0 33.0 41.0 30-31 37.7360134819737 39.0 36.5 41.0 33.0 41.0 32-33 37.413146297262415 39.0 35.0 41.0 33.0 41.0 34-35 37.16725301353689 39.0 35.0 41.0 33.0 41.0 36-37 36.872322825997415 39.0 35.0 41.0 32.5 41.0 38-39 36.63872909744913 39.0 35.0 41.0 32.0 41.0 40-41 36.31400848458222 39.0 35.0 41.0 31.0 41.0 42-43 35.876608020538725 39.0 35.0 41.0 30.5 41.0 44-45 35.22171462698042 38.0 35.0 40.0 27.5 41.0 46-47 34.37686200884154 38.0 34.5 40.0 21.5 41.0 48-49 33.70000720777617 38.0 34.0 40.0 11.5 41.0 50-51 33.30670803701365 38.0 33.0 40.0 3.5 41.0 52-53 32.994566709684506 38.0 33.0 40.0 2.0 41.0 54-55 32.69789121063181 38.0 33.0 40.0 2.0 41.0 56-57 32.38412984980367 37.0 33.0 40.0 2.0 41.0 58-59 32.10228177599605 37.0 33.0 40.0 2.0 41.0 60-61 31.723219679288285 36.0 32.0 40.0 2.0 41.0 62-63 31.30254640434938 36.0 31.0 39.0 2.0 41.0 64-65 30.888765136329937 35.0 31.0 39.0 2.0 40.5 66-67 30.418845588840988 35.0 30.5 39.0 2.0 40.0 68-69 29.96646839561767 35.0 29.5 38.0 2.0 40.0 70-71 29.523375504544333 35.0 29.0 37.5 2.0 40.0 72-73 29.051952620884705 35.0 29.0 37.0 2.0 39.5 74-75 28.591981520634832 34.0 28.0 36.5 2.0 39.0 76-77 27.340780224608032 32.5 26.0 35.0 2.0 37.5 78-79 27.883723125291745 34.0 27.0 35.5 2.0 38.0 80-81 27.696077253631344 34.0 27.0 35.0 2.0 37.0 82-83 27.33599735028419 34.0 26.0 35.0 2.0 37.0 84-85 26.948071405035833 34.0 25.5 35.0 2.0 36.0 86-87 26.51080479969247 34.0 24.5 35.0 2.0 36.0 88-89 26.08058122134051 33.0 23.5 35.0 2.0 35.5 90-91 25.58172588484033 33.0 20.0 35.0 2.0 35.0 92-93 25.141886789862433 33.0 18.0 35.0 2.0 35.0 94-95 24.67575818940663 32.0 9.0 35.0 2.0 35.0 96-97 24.15670563442159 32.0 2.0 34.0 2.0 35.0 98-99 23.659969727340126 32.0 2.0 34.0 2.0 35.0 100-101 22.03102432796068 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13252452253602343 1101 2 0.2177236879108051 1101 3 0.15829208413813234 1101 4 0.1387840574918613 1101 5 0.08258211343709831 1101 6 0.08185133224026231 1101 7 0.14007586410066608 1101 8 -0.012831264582636948 1101 9 -0.04129413489927458 1101 10-11 -0.06853237345200824 1101 12-13 -0.09285955817287572 1101 14-15 -0.04244423689117838 1101 16-17 -0.04239058152596442 1101 18-19 -0.08173829308321956 1101 20-21 0.0652659770291848 1101 22-23 -0.14419165817604807 1101 24-25 -0.11983263087665819 1101 26-27 -0.03193979115349066 1101 28-29 0.12880990545266258 1101 30-31 0.17371198331425575 1101 32-33 0.07043912852994794 1101 34-35 0.35682309576937854 1101 36-37 0.543692298224677 1101 38-39 0.3225877393554981 1101 40-41 0.41955780366124173 1101 42-43 0.6330091661105897 1101 44-45 0.42156996991565876 1101 46-47 0.5311365800641639 1101 48-49 0.5996879839968585 1101 50-51 0.4612186910643885 1101 52-53 0.5599799797734164 1101 54-55 0.7006423914540605 1101 56-57 0.7635961967739 1101 58-59 0.6182916847523359 1101 60-61 0.7336985959327329 1101 62-63 0.43750639711326045 1101 64-65 0.7085097607257218 1101 66-67 0.7160083464619404 1101 68-69 0.9107257617054536 1101 70-71 0.9063671689815074 1101 72-73 0.9459995276455508 1101 74-75 1.1582776565826371 1101 76-77 1.1820155940677708 1101 78-79 1.0632378657864905 1101 80-81 0.965524894165867 1101 82-83 1.4065428353477643 1101 84-85 1.1769630110004314 1101 86-87 0.9537709580721376 1101 88-89 0.917492191326911 1101 90-91 0.9496271670929453 1101 92-93 1.3775884153095568 1101 94-95 1.266359400906552 1101 96-97 1.2405567964522675 1101 98-99 1.0392682921868435 1101 100-101 1.7502056826359755 1102 1 0.1538880266431022 1102 2 0.012716861437517935 1102 3 0.06066232519097525 1102 4 0.17607226895585626 1102 5 0.12499017986652206 1102 6 0.16784251377968928 1102 7 0.08831217198580532 1102 8 0.1348891409770161 1102 9 0.16324826757168154 1102 10-11 0.2340762346188967 1102 12-13 0.1405918395755137 1102 14-15 0.02794957228092443 1102 16-17 0.10836536645854977 1102 18-19 -0.11929508710277759 1102 20-21 -0.07957835699799176 1102 22-23 -0.2535802971339436 1102 24-25 -0.1096013141506802 1102 26-27 0.05939226641270068 1102 28-29 -0.004018156948013996 1102 30-31 0.1409253273908888 1102 32-33 0.10305792035608619 1102 34-35 0.16681772411826756 1102 36-37 0.13852152448309596 1102 38-39 0.2634240382878801 1102 40-41 0.5598474251836549 1102 42-43 0.32059169929172526 1102 44-45 0.5427062979444912 1102 46-47 0.5373167765770646 1102 48-49 0.7124450958391151 1102 50-51 0.9135201078373711 1102 52-53 0.5536654919827271 1102 54-55 0.6667953939832074 1102 56-57 0.6453303448076504 1102 58-59 0.5004287066194308 1102 60-61 0.7946388586960005 1102 62-63 0.6335548762645402 1102 64-65 1.0612509814334352 1102 66-67 0.9108398109083247 1102 68-69 0.9995174759336187 1102 70-71 1.0810885145801095 1102 72-73 1.1279865909191251 1102 74-75 1.0766677279808299 1102 76-77 1.2618741405885032 1102 78-79 1.0750778801108964 1102 80-81 1.4039131750136917 1102 82-83 1.3829439410126128 1102 84-85 1.5474250849053988 1102 86-87 1.637433864583027 1102 88-89 1.881611240544668 1102 90-91 1.810129304562448 1102 92-93 2.12242939205478 1102 94-95 1.8839901670382915 1102 96-97 1.9329374450791406 1102 98-99 1.5944396254754132 1102 100-101 1.6617496966022678 1103 1 -0.05318104014334324 1103 2 0.03850893551415169 1103 3 0.04585390864811245 1103 4 0.060082219548405646 1103 5 -0.12534668915189684 1103 6 -0.08745928631014266 1103 7 -0.019620661514522908 1103 8 -0.027229506018798588 1103 9 -0.11549270606615636 1103 10-11 -0.04174175245915279 1103 12-13 -0.0705645713010341 1103 14-15 -0.10258803612180145 1103 16-17 -0.21631590362402875 1103 18-19 -0.32522916442717786 1103 20-21 -0.25103432217091637 1103 22-23 -0.25757780948581654 1103 24-25 -0.12056078621646549 1103 26-27 0.07756591807286384 1103 28-29 -0.10000336926187003 1103 30-31 -0.03405653350812088 1103 32-33 -0.26387881736978613 1103 34-35 -0.10293143802801552 1103 36-37 -0.17881945606487903 1103 38-39 -0.20192682375129323 1103 40-41 0.17190560850949765 1103 42-43 -0.12722469845557072 1103 44-45 0.11821160147210463 1103 46-47 0.26650172630584024 1103 48-49 0.43026287561314547 1103 50-51 0.14154933770296907 1103 52-53 0.1453145953928825 1103 54-55 0.16711671520076976 1103 56-57 0.4315359903868945 1103 58-59 0.5838409306459624 1103 60-61 0.558037607961797 1103 62-63 0.7322765015279913 1103 64-65 0.6703205319292387 1103 66-67 0.6052875184714637 1103 68-69 0.7229670146821903 1103 70-71 0.8158240292123189 1103 72-73 0.9555372940252269 1103 74-75 1.0738604861607008 1103 76-77 1.2249843917718657 1103 78-79 0.9396651723750011 1103 80-81 0.986917701151274 1103 82-83 1.2019830869200874 1103 84-85 1.1677045998140123 1103 86-87 0.9194346937990723 1103 88-89 1.0145933087476884 1103 90-91 1.1032709968541958 1103 92-93 1.3019304804008414 1103 94-95 1.0804141728428043 1103 96-97 1.2396454573791793 1103 98-99 1.3836598168861194 1103 100-101 1.6444674521908418 1104 1 0.08663442156375822 1104 2 0.1377886098016532 1104 3 -0.14659110327701086 1104 4 0.1548038461835901 1104 5 -0.04252127779532344 1104 6 -0.01411133767249595 1104 7 -0.0828551407840763 1104 8 0.14602448709112537 1104 9 -0.10034418405888346 1104 10-11 -0.024323843090101605 1104 12-13 -0.12555264617636652 1104 14-15 -0.18590249804572068 1104 16-17 -0.12588973098676348 1104 18-19 -0.15830632841571202 1104 20-21 -0.3805219031774456 1104 22-23 -0.22881055419178153 1104 24-25 0.052934118502989236 1104 26-27 -0.13813429152023105 1104 28-29 -0.004004911096167518 1104 30-31 0.007332081009217006 1104 32-33 0.12802414343389756 1104 34-35 0.40698930205244466 1104 36-37 0.32636978000440564 1104 38-39 0.3396472927053722 1104 40-41 0.118919509109368 1104 42-43 0.3083724009686506 1104 44-45 0.3175466854298108 1104 46-47 -0.23474860494761884 1104 48-49 0.2547611771863032 1104 50-51 0.1933983343061172 1104 52-53 0.38150915560873955 1104 54-55 0.4493470748818069 1104 56-57 0.6762130483743185 1104 58-59 0.782711055197268 1104 60-61 0.5779874983470137 1104 62-63 0.12839782461454874 1104 64-65 0.3192183613156345 1104 66-67 0.6976963936551606 1104 68-69 0.4238174326638351 1104 70-71 0.3172068618510231 1104 72-73 0.43924203275658513 1104 74-75 0.584171503048605 1104 76-77 0.4819672147741052 1104 78-79 0.6870693497823126 1104 80-81 0.33307877465340496 1104 82-83 0.39131311173398586 1104 84-85 0.6783890296966888 1104 86-87 0.5681470212461299 1104 88-89 1.0154075899878983 1104 90-91 1.1077591801397482 1104 92-93 1.039748784864937 1104 94-95 1.3209090052887795 1104 96-97 1.3919040037185262 1104 98-99 1.457398042331203 1104 100-101 1.6383266152639102 1105 1 0.07479517165761251 1105 2 0.17314154397615766 1105 3 -0.04848922895801877 1105 4 0.052114605891681265 1105 5 0.18436563127227856 1105 6 -0.05291624185057486 1105 7 0.14873816770483472 1105 8 0.011397656951857016 1105 9 0.11492322916115683 1105 10-11 0.07069689619917341 1105 12-13 0.03087561785812909 1105 14-15 0.017388318981552686 1105 16-17 0.1733054197736763 1105 18-19 0.07954997074281067 1105 20-21 -0.12289689516557445 1105 22-23 -0.02119332913615324 1105 24-25 0.391698734437 1105 26-27 0.2939525479585825 1105 28-29 0.33014029139803114 1105 30-31 0.25922974886927364 1105 32-33 0.31857380327291906 1105 34-35 0.21663650522422984 1105 36-37 0.10407023690971329 1105 38-39 0.43467261020420267 1105 40-41 0.6081790294961351 1105 42-43 0.39152642109446134 1105 44-45 0.9481080654852931 1105 46-47 0.9536080925151396 1105 48-49 0.9728487674914916 1105 50-51 0.684196133102958 1105 52-53 0.9797071723283608 1105 54-55 1.3639497320746372 1105 56-57 1.2837231433263874 1105 58-59 1.2548114461763618 1105 60-61 1.2642748450914532 1105 62-63 1.1888988638681184 1105 64-65 1.2134617206226785 1105 66-67 1.2814455810229646 1105 68-69 1.2083394218043217 1105 70-71 0.9374626922008886 1105 72-73 0.8893029229436529 1105 74-75 1.1560265019956155 1105 76-77 1.2382828548535834 1105 78-79 1.3481307618852654 1105 80-81 0.8295215058300975 1105 82-83 0.993865005739277 1105 84-85 0.7481151612011807 1105 86-87 0.934760885895944 1105 88-89 0.7437732607413814 1105 90-91 0.43956949576151416 1105 92-93 0.9308560706276197 1105 94-95 0.7991258842317635 1105 96-97 0.8629285154524808 1105 98-99 1.3855979818988367 1105 100-101 1.3480177692474093 1106 1 -0.31493189016996936 1106 2 -0.028673891899721582 1106 3 -0.3163516544177156 1106 4 -0.09189025187841082 1106 5 -0.24758666293421072 1106 6 -0.11415217066555527 1106 7 -0.29585678457380027 1106 8 0.018217218818364245 1106 9 -0.3246115459334291 1106 10-11 -0.3280061414641082 1106 12-13 -0.4135247122211183 1106 14-15 -0.30809784727251355 1106 16-17 -0.1887463653011352 1106 18-19 -0.041434973283841714 1106 20-21 -0.1909835253989698 1106 22-23 0.12193962118068669 1106 24-25 -0.05706891779648515 1106 26-27 -0.08031372634972911 1106 28-29 -0.47300324636645286 1106 30-31 -0.36554643560143063 1106 32-33 -0.649975249306209 1106 34-35 -0.4295792607308968 1106 36-37 -0.2546418312236227 1106 38-39 -0.4785725981972462 1106 40-41 -0.7231458027395306 1106 42-43 -0.7737856750644809 1106 44-45 -1.0397786488248855 1106 46-47 -1.104093497158992 1106 48-49 -1.7390091738727342 1106 50-51 -1.3624119912061303 1106 52-53 -1.4753973787171546 1106 54-55 -1.2881124847500658 1106 56-57 -1.3767389676238402 1106 58-59 -1.3235123117447642 1106 60-61 -1.1697326595405606 1106 62-63 -1.021752419859908 1106 64-65 -1.2843351560802674 1106 66-67 -1.333420441054976 1106 68-69 -1.212721814124496 1106 70-71 -1.1282339774240349 1106 72-73 -1.26106997319037 1106 74-75 -0.9525415457101758 1106 76-77 -1.0568188936322542 1106 78-79 -1.201132009223457 1106 80-81 -1.22986161964673 1106 82-83 -1.2634177569617684 1106 84-85 -0.8198624806005554 1106 86-87 -0.703989172490715 1106 88-89 -0.8346672704993736 1106 90-91 -0.7466450246115386 1106 92-93 -1.3101952006445892 1106 94-95 -1.4185084440102536 1106 96-97 -1.004736173852642 1106 98-99 -1.0600868546963653 1106 100-101 -0.9403344813637062 1107 1 0.056828206273671356 1107 2 0.05330320273560574 1107 3 0.10041547437472786 1107 4 0.19643604910611856 1107 5 0.019090049945035048 1107 6 0.09802383897881128 1107 7 0.15632324355559746 1107 8 0.24817502472553343 1107 9 0.03634887198901282 1107 10-11 0.17633884547581857 1107 12-13 -0.023003313788322544 1107 14-15 -0.033459510925318625 1107 16-17 0.32388674701551423 1107 18-19 0.25271813099584506 1107 20-21 0.23522704321666055 1107 22-23 0.18603445742741798 1107 24-25 0.35834197229794995 1107 26-27 0.2797927635035933 1107 28-29 0.19147836906452653 1107 30-31 0.46582788627921445 1107 32-33 0.05860923934157114 1107 34-35 0.1996652640832437 1107 36-37 0.18842125899136875 1107 38-39 -0.3639201971524386 1107 40-41 -0.7376374643830914 1107 42-43 -0.4247551345782412 1107 44-45 0.1856834769536917 1107 46-47 0.5879649717982218 1107 48-49 0.6336801972343622 1107 50-51 0.7357395056795468 1107 52-53 0.8321423038225362 1107 54-55 0.7711350539278499 1107 56-57 1.1151236001880953 1107 58-59 0.7983231312932162 1107 60-61 0.9837734686491153 1107 62-63 1.2067486852234737 1107 64-65 1.3141177271827438 1107 66-67 1.8511535995317203 1107 68-69 1.607903060297847 1107 70-71 1.4705705232792 1107 72-73 1.1451235168638796 1107 74-75 1.2748544759896454 1107 76-77 1.4059598887667804 1107 78-79 1.30788446656517 1107 80-81 1.6123877273494536 1107 82-83 1.4646814996185 1107 84-85 1.3539621125278352 1107 86-87 1.201300158431529 1107 88-89 1.2011474773406903 1107 90-91 1.6927763135439768 1107 92-93 1.676367990032528 1107 94-95 1.4258757231587254 1107 96-97 1.6158937236759883 1107 98-99 1.7889688013213139 1107 100-101 1.5837261977602672 1108 1 -0.04942457521275401 1108 2 -0.20348362414468113 1108 3 0.01181586007417934 1108 4 -0.12651306823552488 1108 5 -0.29514374326071646 1108 6 -0.09601265412310767 1108 7 -0.08303293505291975 1108 8 -0.01194958586636119 1108 9 0.03983500164336817 1108 10-11 -0.12074244810080614 1108 12-13 -0.15245369207048043 1108 14-15 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0.2890821133187451 2316 88-89 0.39859944058212804 2316 90-91 0.46019485990989395 2316 92-93 0.2561716658888322 2316 94-95 0.6311806521478616 2316 96-97 0.934126806382757 2316 98-99 1.3487850085059456 2316 100-101 1.1454638752874153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 1.0 5 13.0 6 17.0 7 71.0 8 143.0 9 262.0 10 532.0 11 856.0 12 1366.0 13 1980.0 14 2517.0 15 3844.0 16 5682.0 17 8019.0 18 6462.0 19 3442.0 20 2709.0 21 2688.0 22 2761.0 23 3020.0 24 3067.0 25 3324.0 26 3626.0 27 4136.0 28 4856.0 29 5798.0 30 6904.0 31 8322.0 32 10207.0 33 13396.0 34 18802.0 35 27063.0 36 36789.0 37 52509.0 38 42185.0 39 3981.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08323375499609 20.830414657931243 16.58254014037112 17.503811446701555 2 15.865345012218896 23.611301793020125 39.81095032812543 20.71240286663555 3 18.517463411955298 29.803467970015653 32.1363848540597 19.542683763969357 4 11.866745380158708 19.388231417666603 37.75639089486258 30.98863230731212 5 11.217536090083595 40.902895789347205 32.53308615273701 15.346481967832194 6 26.78066393915264 39.55284329608171 17.32612098080672 16.340371783958922 7 24.559295971882808 38.41332820780362 20.272042065954583 16.75533375435899 8 27.263928169362146 34.056055904884815 19.14248057332711 19.53753535242593 9 28.466597105906256 15.988220434388642 20.259342650814137 35.28583980889096 10-11 24.58400834729125 29.37666465306571 25.160802053872978 20.878524945770067 12-13 26.992089821458915 27.991459959119013 27.09575108938725 17.920699130034823 14-15 23.2028611439084 27.586733573134904 29.04013015184382 20.17027513111288 16-17 18.6156264587166 31.32568164968835 29.205394162387766 20.85329772920728 18-19 18.561739751228753 26.963260935226117 36.203973200801784 18.27102611274335 20-21 20.401095581976442 25.701556879650735 36.65308630110656 17.24426123726626 22-23 19.708460114055455 26.418442454852332 36.530695951082805 17.342401480009404 24-25 19.04318159196296 26.66371490058383 36.76458979032164 17.52851371713157 26-27 18.59130903368303 27.10310847661821 36.746542371077936 17.55904011862083 28-29 18.200805583825165 27.625140942845423 36.603779413506146 17.570274059823266 30-31 18.52261182349872 27.311980010434112 36.52128696559488 17.64412120047228 32-33 18.239620802328453 27.58381613992696 36.20088415387572 17.97567890386886 34-35 18.569119141107663 27.783402894093744 36.04780471731788 17.59967324748071 36-37 18.65046404349378 27.839005738762733 35.746107800873176 17.764422416870314 38-39 18.67294544056674 28.085957879129026 35.64382602487712 17.597270655427113 40-41 18.623863917186085 28.147395590213897 35.45813998187759 17.770600510722424 42-43 18.385590187486162 28.664116044716557 34.85735569504448 18.0929380727528 44-45 18.66728218786897 28.827672368818476 34.23710837749526 18.267937065817293 46-47 19.03178972514347 29.306646256075126 33.04885499327274 18.612709025508664 48-49 19.14248057332711 29.773950410500017 32.04525796974107 19.038311046431808 50-51 19.329196298635328 30.238508745435077 31.445639638650153 18.986655317279443 52-53 19.36420549713062 30.639570004667892 30.804319174057497 19.191905324143992 54-55 18.948900299294323 30.41647217111947 30.96700897882973 19.667618550756476 56-57 19.28818062000604 30.46006205552047 30.516351355061914 19.73540596941157 58-59 19.2452771904775 29.89768390126033 30.88720859990664 19.96983030835553 60-61 19.192248551580217 29.93715505642659 31.49300502484967 19.377591367143523 62-63 19.42118125154452 29.885670940992338 31.41286141849035 19.28028638897279 64-65 19.30551360553557 29.745977374447403 31.49815343639309 19.45035558362393 66-67 19.176803316949943 29.951570608748177 31.094003130234217 19.77762294406766 68-69 19.32730854773607 29.63236909305582 31.17431835031165 19.866004008896454 70-71 19.437656168483482 29.835731349021117 30.83400834729125 19.892604135204152 72-73 19.230861638155908 29.79969246821714 30.66582690353936 20.303618990087593 74-75 18.937058952744444 30.10035970235317 30.304065185754688 20.6585161591477 76-77 18.590742469590047 29.403608006809634 31.203664296109174 20.801985227491144 78-79 18.45911474779648 29.57590817979626 30.867644636041625 21.097332436365633 80-81 18.320107636124 29.963240341579944 30.568178697932396 21.148473324363657 82-83 18.212193862053226 29.82394118444559 30.26481758288528 21.699047370615908 84-85 18.240198248847612 29.471153351112577 30.185412097435737 22.103236302604078 86-87 18.168057881874848 29.410644169252315 29.8173686811829 22.603929267689942 88-89 18.0245888135314 28.820292978939566 30.172609077679237 22.982509129849802 90-91 18.247210527309456 29.185410469983886 29.967200847182724 22.60017815552394 92-93 18.12882056351274 29.443866676277068 29.59299950918308 22.834313251027112 94-95 17.96366594360087 29.51086658063099 29.0459650182597 23.479502457508445 96-97 18.327315412284797 29.388162772179356 28.635636618248718 23.648885197287132 98-99 18.5717570902451 29.33145930509935 28.37316501402089 23.723618590634665 100-101 18.558612060368784 29.186655430049484 27.910178006553167 24.344554503028565 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1155.0 1 777.5 2 502.0 3 611.5 4 570.5 5 568.0 6 854.0 7 1545.0 8 2208.5 9 2490.0 10 2246.5 11 1875.5 12 1767.5 13 1780.0 14 1803.0 15 1736.5 16 1683.0 17 1704.5 18 1716.5 19 1732.5 20 1822.5 21 1963.0 22 2140.0 23 2326.5 24 2601.0 25 2837.5 26 3180.0 27 3720.0 28 4192.5 29 4681.5 30 5095.0 31 5474.0 32 6009.5 33 6242.5 34 6547.0 35 7018.5 36 7657.5 37 8241.0 38 8471.0 39 8316.5 40 8177.5 41 8315.0 42 8177.0 43 8229.5 44 8536.5 45 8756.5 46 8649.0 47 8684.0 48 9498.5 49 9344.5 50 8520.5 51 7982.5 52 7255.5 53 6950.0 54 6611.5 55 6113.5 56 5525.0 57 4998.5 58 4781.5 59 4276.0 60 3409.0 61 2764.0 62 2281.0 63 1704.5 64 1177.5 65 810.0 66 584.5 67 471.5 68 348.5 69 239.0 70 184.0 71 141.5 72 121.0 73 106.5 74 83.0 75 73.5 76 67.0 77 45.5 78 26.5 79 17.5 80 9.5 81 6.5 82 6.0 83 3.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04256020209231445 2 0.0 3 0.0 4 0.0 5 0.022996238227298935 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0063497075702243335 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.580685905708559E-4 24-25 3.432274362283424E-4 26-27 0.0012012960267991982 28-29 0.003603888080397595 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01733298552953129 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.716137181141712E-4 84-85 3.432274362283424E-4 86-87 0.0 88-89 0.0 90-91 0.011498119113649468 92-93 3.432274362283424E-4 94-95 0.0 96-97 0.0 98-99 3.432274362283424E-4 100-101 0.01458716603970455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 291352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.526303025124115 #Duplication Level Percentage of deduplicated Percentage of total 1 80.51084646896486 48.73023890189812 2 11.410123902140084 13.812252337102848 3 3.5762380880845686 6.493694106281914 4 1.5456825316209535 3.7421779715812327 5 0.820427952294263 2.482873542542232 6 0.4986806155126217 1.810997642836341 7 0.3348142518204364 1.4185548203969764 8 0.2391960799347851 1.1582123533243691 9 0.17651075054458343 0.9615188857188196 >10 0.8150565813433356 8.75590904473669 >50 0.046190905487387066 1.9145811144245233 >100 0.022240065605038214 2.5482877893990006 >500 0.0017107742773106317 0.8604742596467164 >1k 0.0017107742773106317 3.439480945599491 >5k 5.702580924368773E-4 1.8707462845107115 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5420 1.8602927043576154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4676 1.6049314918037287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3368 1.1559900052170569 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1921 0.6593399049946457 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 913 0.31336664927647656 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 895 0.3071885554243664 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 685 0.23511079381641453 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 496 0.1702408083692578 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.16406271451714763 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 395 0.13557483731019523 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 298 0.10228177599604601 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.864548724566847E-4 0.0 24-25 0.0 0.0 0.0 0.0027458194898267387 0.0 26-27 0.0 0.0 0.0 0.0036038880803975944 0.0 28-29 0.0 0.0 0.0 0.006521321288338504 0.0 30-31 0.0 0.0 0.0 0.01458716603970455 0.0 32-33 0.0 0.0 0.0 0.02059364617370054 0.0 34-35 0.0 0.0 0.0 0.02745819489826739 0.0 36-37 0.0 0.0 0.0 0.034151129904720065 0.0 38-39 0.0 0.0 0.0 0.04170213350174359 0.0 40-41 0.0 0.0 0.0 0.060751256212416595 0.0 42-43 0.0 0.0 0.0 0.09867788791564842 0.0 44-45 0.0 0.0 0.0 0.15393750514841154 0.0 46-47 0.0 0.0 0.0 0.2136590790521431 0.0 48-49 0.0 0.0 0.0 0.33481836404074794 0.0 50-51 0.0 0.0 0.0 0.4868681182899036 0.0 52-53 0.0 0.0 0.0 0.6378881902303742 0.0 54-55 0.0 0.0 0.0 0.8436530382492655 0.0 56-57 0.0 0.0 0.0 1.067780554106373 0.0 58-59 0.0 0.0 0.0 1.3691342431148577 0.0 60-61 0.0 0.0 0.0 1.7027513111288064 0.0 62-63 0.0 0.0 0.0 2.12955462807875 0.0 64-65 0.0 0.0 0.0 2.505388670748785 0.0 66-67 0.0 0.0 0.0 2.939742991295752 0.0 68-69 0.0 0.0 0.0 3.516021856723139 0.0 70-71 0.0 0.0 0.0 4.134517696806612 0.0 72-73 0.0 0.0 0.0 4.851519811087619 0.0 74-75 0.0 0.0 0.0 5.624982838628188 0.0 76-77 0.0 0.0 0.0 6.387805815645679 0.0 78-79 0.0 0.0 0.0 7.169163074219501 0.0 80-81 0.0 0.0 0.0 8.089699058183914 0.0 82-83 0.0 0.0 0.0 9.206389521952826 0.0 84-85 0.0 0.0 0.0 10.314499299816031 0.0 86-87 0.0 0.0 0.0 11.409223207666328 0.0 88-89 0.0 0.0 0.0 12.775783245009473 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGCGG 20 5.386844E-4 47.498455 10-11 ATTGGCG 40 5.3823827E-4 47.498455 7 GGTATCA 2730 0.0 42.808155 1 CAGTGCG 50 0.0016140941 37.998764 9 CCTACCT 65 1.3260054E-4 36.537273 3 GTATCAA 4355 0.0 34.252754 1 GTGCATA 90 2.4045412E-5 31.671072 1 ATACGTC 60 0.0039467826 31.665636 5 GTAGGAC 95 3.4827703E-5 29.999025 3 TGTAGGA 175 1.5097612E-10 29.856173 2 GCTTATA 115 3.847388E-6 28.917065 1 ATATTGG 85 6.39683E-4 27.940268 5 TCGTTCA 35 0.008389564 27.141973 56-57 GCCTTCG 35 0.008389564 27.141973 74-75 GACGTGG 105 6.8899746E-5 27.141973 7 ACCTCGG 70 0.0083828 27.141973 6 CCATATA 70 0.0083828 27.141973 2 TGTAAGG 70 0.0083828 27.141973 2 GATAGTC 70 0.0083828 27.141973 7 TATCAAC 5595 0.0 26.487074 2 >>END_MODULE