FastQCFastQC Report
Thu 26 May 2016
SRR937180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429573
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80251.8681341704436731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58951.372292951372642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43211.0058825857304812No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14480.3370789132464098No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.280278322892733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10830.2521108170206228No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6660.15503767694897028No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6410.14921794433076566No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.14619168336929927No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5520.12849969620995733No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.1273357496863164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.053.486261
GTATCAA49600.046.4580421
TATCAAC66300.034.5369262
ATCAACG66500.034.4330563
TCAACGC67600.033.8767054
CAACGCA68450.033.243925
AACGCAG69150.032.9760936
CGCAGAG76950.029.506578
ACGCAGA76850.029.4831587
GAGCGCA650.005839652429.2311619
CCTATAC1153.8532253E-628.9168073
GTACATG41550.028.5870821
TACATGG40800.028.4105592
GCAGAGT79450.027.9204549
CTATACT1205.3610456E-627.7151744
ACATGGG42100.026.6305453
CATGGGA30500.026.4819474
ATGGGAG9250.026.1925985
ATTGGAC1309.991856E-625.5802523
AGTACTT52450.025.44542112-13
Produced by FastQC (version 0.11.5)