##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937180_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 429573 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.969821194535037 33.0 31.0 34.0 31.0 34.0 2 32.03294899819123 33.0 31.0 34.0 30.0 34.0 3 32.0202992273723 33.0 31.0 34.0 30.0 34.0 4 35.69063232558843 37.0 35.0 37.0 35.0 37.0 5 35.73170101472858 37.0 35.0 37.0 35.0 37.0 6 35.80158203611493 37.0 35.0 37.0 35.0 37.0 7 35.68575073386828 37.0 35.0 37.0 35.0 37.0 8 35.70869910352839 37.0 35.0 37.0 35.0 37.0 9 37.35401200727234 39.0 37.0 39.0 35.0 39.0 10-11 37.26346278746569 39.0 37.0 39.0 34.5 39.0 12-13 37.11574400625737 39.0 37.0 39.0 34.0 39.0 14-15 38.199443400772395 40.0 38.0 41.0 33.5 41.0 16-17 38.18894809496872 40.0 38.0 41.0 33.5 41.0 18-19 38.30740875241228 40.0 38.0 41.0 34.0 41.0 20-21 38.285062143104895 40.0 38.0 41.0 34.0 41.0 22-23 38.14876866097264 40.0 38.0 41.0 34.0 41.0 24-25 38.05694259182956 40.0 38.0 41.0 34.0 41.0 26-27 37.997083150011754 40.0 38.0 41.0 34.0 41.0 28-29 37.85833606860766 40.0 37.5 41.0 33.0 41.0 30-31 37.486033805662835 39.5 36.5 41.0 33.0 41.0 32-33 37.066575413259216 39.0 35.0 41.0 33.0 41.0 34-35 36.97419181373131 39.0 35.0 41.0 33.0 41.0 36-37 36.788556310568865 39.0 35.0 41.0 32.5 41.0 38-39 36.59814280692688 39.0 35.0 41.0 31.5 41.0 40-41 36.25815286342484 39.0 35.0 41.0 30.5 41.0 42-43 35.82183586957281 39.0 35.0 41.0 30.0 41.0 44-45 35.31626638545718 39.0 35.0 41.0 27.0 41.0 46-47 34.612437234183716 38.5 35.0 40.5 22.5 41.0 48-49 33.99345862053714 38.0 34.5 40.0 14.5 41.0 50-51 32.89120242659571 37.5 33.0 39.5 7.5 40.5 52-53 32.94489760762431 38.0 33.0 39.5 4.0 40.5 54-55 33.25403365667768 38.0 34.0 40.0 2.0 41.0 56-57 33.013650764829265 38.0 33.5 40.0 2.0 41.0 58-59 32.736103060480986 38.0 33.0 40.0 2.0 41.0 60-61 32.40542236127503 37.0 33.0 40.0 2.0 41.0 62-63 32.06928158892668 36.5 33.0 40.0 2.0 41.0 64-65 31.624602803248806 36.0 32.0 39.5 2.0 41.0 66-67 31.251561434261465 35.5 31.5 39.0 2.0 41.0 68-69 30.772937312168132 35.0 31.0 39.0 2.0 40.5 70-71 30.290959860140184 35.0 30.5 38.5 2.0 40.0 72-73 29.84095369122361 35.0 30.0 37.5 2.0 40.0 74-75 29.384688981849415 35.0 29.0 37.0 2.0 39.0 76-77 28.94241141784982 35.0 29.0 36.5 2.0 39.0 78-79 28.46204603175712 34.0 28.5 36.0 2.0 39.0 80-81 28.00676718508845 34.0 27.0 35.5 2.0 37.5 82-83 27.532797685143155 34.0 26.0 35.0 2.0 37.0 84-85 27.010544191557663 34.0 25.5 35.0 2.0 36.5 86-87 26.56742742211452 33.5 24.5 35.0 2.0 36.0 88-89 26.120577876170056 33.0 23.5 35.0 2.0 36.0 90-91 25.60770462761859 33.0 19.5 35.0 2.0 35.0 92-93 25.138503816580652 33.0 17.5 35.0 2.0 35.0 94-95 24.637711168998052 32.0 6.0 35.0 2.0 35.0 96-97 23.983438204915114 32.0 2.0 35.0 2.0 35.0 98-99 23.16388599842169 31.5 2.0 34.0 2.0 35.0 100-101 20.964660255649214 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10418359028198054 1101 2 -0.061531338478467035 1101 3 0.07359795117782753 1101 4 0.14773016029406705 1101 5 0.08067648194299437 1101 6 -0.1680426306375722 1101 7 0.0035587791057665186 1101 8 -0.11475403520937277 1101 9 -0.14315122659483137 1101 10-11 -0.06633122974704264 1101 12-13 -0.07701269707209946 1101 14-15 0.0013365310461495028 1101 16-17 0.04510984376647542 1101 18-19 -0.12467502023662291 1101 20-21 0.1116878840619151 1101 22-23 -0.11564492733454301 1101 24-25 -0.038906778058795055 1101 26-27 -0.012704059285383096 1101 28-29 -0.3422073083288666 1101 30-31 -0.1183271223632616 1101 32-33 0.021069595596976853 1101 34-35 0.08087123483770142 1101 36-37 0.144600703925029 1101 38-39 0.23225638949295302 1101 40-41 0.23356045495751943 1101 42-43 0.26713980310612584 1101 44-45 0.36843576523822463 1101 46-47 0.6622317060921148 1101 48-49 0.9998419768177271 1101 50-51 1.171652875269551 1101 52-53 0.973257261552746 1101 54-55 1.0500040077448602 1101 56-57 1.1502451399741034 1101 58-59 1.11972460046006 1101 60-61 1.1193972346792087 1101 62-63 0.8527165888406039 1101 64-65 1.1182811257830707 1101 66-67 1.2814634964279996 1101 68-69 1.3866543668457645 1101 70-71 1.1765684873144444 1101 72-73 1.1956605425787217 1101 74-75 1.1062295118300405 1101 76-77 1.2567009346192037 1101 78-79 1.1264732938577744 1101 80-81 1.0048390330703683 1101 82-83 1.1926016244301252 1101 84-85 1.3767531625341185 1101 86-87 1.3797596505827663 1101 88-89 1.357030730781183 1101 90-91 1.1602396758305353 1101 92-93 1.1288295060920923 1101 94-95 0.9714400282595932 1101 96-97 0.8464144324914891 1101 98-99 0.6338545601976229 1101 100-101 0.9997475336601234 1102 1 -0.10695866896704231 1102 2 -0.2128370596456044 1102 3 -0.30039028272275203 1102 4 -0.21047412762001727 1102 5 -0.215296985107976 1102 6 -0.34042941154332595 1102 7 -0.09520696496183234 1102 8 -0.19513623602273356 1102 9 -0.3661827739553587 1102 10-11 -0.15267623705150157 1102 12-13 -0.008685615301658345 1102 14-15 -0.026530962142935266 1102 16-17 -0.03937785710611763 1102 18-19 -0.1595247853094719 1102 20-21 -0.13115572157634148 1102 22-23 -0.4068535189561331 1102 24-25 -0.26116760642378267 1102 26-27 -0.40885994036091233 1102 28-29 -0.21963534954969077 1102 30-31 -0.0978454229910497 1102 32-33 0.14873967101056706 1102 34-35 0.022891213516579967 1102 36-37 0.09142504158839415 1102 38-39 0.15901992248146257 1102 40-41 0.12427392279816019 1102 42-43 0.2258700119741306 1102 44-45 0.5199215314166068 1102 46-47 0.5797860946892612 1102 48-49 0.43857297535682704 1102 50-51 0.47816529284762765 1102 52-53 0.2840378514370556 1102 54-55 0.5416548151835627 1102 56-57 0.5152927176579993 1102 58-59 0.30491194992900716 1102 60-61 0.5447725258704317 1102 62-63 0.7776086013174073 1102 64-65 0.7052791910888772 1102 66-67 0.6068893265697426 1102 68-69 0.6543149662061296 1102 70-71 0.3636368333483233 1102 72-73 0.596868739364762 1102 74-75 0.48976171066922447 1102 76-77 0.6555132692818155 1102 78-79 0.7867259886104101 1102 80-81 0.5927396924457398 1102 82-83 0.8038196921049376 1102 84-85 0.5818552275240485 1102 86-87 0.4976599151225862 1102 88-89 0.5875688916371864 1102 90-91 0.405820281508273 1102 92-93 0.3144069530854203 1102 94-95 0.215822702875613 1102 96-97 0.46182960207335455 1102 98-99 0.5804183063973269 1102 100-101 0.5138163140131056 1103 1 -0.085076112047755 1103 2 -0.11941551472613554 1103 3 -0.0372155781555179 1103 4 -0.05442904845772034 1103 5 -0.14555568839766408 1103 6 -0.34376745963384536 1103 7 -0.020277001919446036 1103 8 -0.16239270360497216 1103 9 -0.17997480753659545 1103 10-11 -0.15988251528300879 1103 12-13 -0.08533479174004555 1103 14-15 -0.3828388509963361 1103 16-17 -0.13575418598323807 1103 18-19 -0.24446159235422726 1103 20-21 -0.19569466394983692 1103 22-23 -0.1954769619455945 1103 24-25 -0.29339373226217447 1103 26-27 -0.2797711052101235 1103 28-29 -0.3605524458676257 1103 30-31 -0.31439148312701803 1103 32-33 -0.15782600859886742 1103 34-35 -0.09313013977082107 1103 36-37 0.04036330868699167 1103 38-39 -0.09940686950649535 1103 40-41 0.0534339355736293 1103 42-43 0.09962280660843703 1103 44-45 0.036151854909782344 1103 46-47 0.3425547217823848 1103 48-49 0.14336651461859873 1103 50-51 0.020012260954580086 1103 52-53 0.04000627777278254 1103 54-55 0.44958747020021406 1103 56-57 0.295200551759379 1103 58-59 0.07645732566473384 1103 60-61 0.13654694755222962 1103 62-63 0.0852903095803974 1103 64-65 0.10725482644042827 1103 66-67 0.5694863973367283 1103 68-69 0.703690336922925 1103 70-71 0.5886913572338912 1103 72-73 0.5496040589796714 1103 74-75 0.5470633486671233 1103 76-77 0.42972415580825896 1103 78-79 0.4277982156487816 1103 80-81 0.6366343860625641 1103 82-83 0.43703956708782954 1103 84-85 0.5755122061251363 1103 86-87 0.8641671925784458 1103 88-89 1.0563766664238834 1103 90-91 0.9633226769091046 1103 92-93 0.7337152525789641 1103 94-95 0.3304560924818567 1103 96-97 0.6606241451221315 1103 98-99 0.5224030670853779 1103 100-101 0.7964863874720649 1104 1 -0.04426497439536092 1104 2 -0.015228639413656708 1104 3 0.0524018428010784 1104 4 0.12948494069691208 1104 5 -0.1140729328713519 1104 6 -0.2177103783106915 1104 7 0.012182178396514587 1104 8 -0.13762527217905074 1104 9 -0.1555834171675201 1104 10-11 -0.08221721746276245 1104 12-13 0.01695905870672476 1104 14-15 -0.06464615559386289 1104 16-17 -0.024685332011095795 1104 18-19 -0.18690808128451408 1104 20-21 -0.10486686084151842 1104 22-23 -0.17827735678870482 1104 24-25 -0.32674601008814363 1104 26-27 -0.114963633958034 1104 28-29 2.4185540108589976E-4 1104 30-31 -0.2181377921558436 1104 32-33 -0.11909484804215253 1104 34-35 0.1459571914914406 1104 36-37 0.07215693906427134 1104 38-39 0.02750057738839473 1104 40-41 0.43664113846914887 1104 42-43 0.14976339468537248 1104 44-45 0.14293683206957297 1104 46-47 0.34467743081633984 1104 48-49 0.7454563200600788 1104 50-51 0.5955057080118422 1104 52-53 0.6717905071236387 1104 54-55 0.9320193020756733 1104 56-57 0.939244574202192 1104 58-59 1.2419239370647617 1104 60-61 1.1364166151404795 1104 62-63 1.4724170995144625 1104 64-65 1.2625640561117777 1104 66-67 1.2965072975277394 1104 68-69 1.4208123836118425 1104 70-71 1.5016506721554563 1104 72-73 1.4098791671983086 1104 74-75 1.4640127583253957 1104 76-77 1.2257101311861156 1104 78-79 1.7268673003733959 1104 80-81 1.8202097246063467 1104 82-83 1.7538723185546026 1104 84-85 1.9862836458068678 1104 86-87 1.703967292571182 1104 88-89 1.8092461160792475 1104 90-91 1.9332532802413418 1104 92-93 1.8720855419169204 1104 94-95 1.98627490051717 1104 96-97 2.190690336894523 1104 98-99 2.437604740468082 1104 100-101 2.41609094611524 1105 1 -0.23959791343424186 1105 2 -0.1276052225858102 1105 3 -0.1347761278612829 1105 4 -0.22843851289623274 1105 5 -0.2277496220767432 1105 6 -0.1696357607496708 1105 7 -0.34858126091677377 1105 8 -0.27877735794342584 1105 9 -0.25343770938233234 1105 10-11 -0.4796740182428323 1105 12-13 -0.15879769358578244 1105 14-15 -0.1943184116508121 1105 16-17 -0.31520308325391966 1105 18-19 -0.2620371594347404 1105 20-21 -0.09113182671373465 1105 22-23 -0.08871390468748785 1105 24-25 0.00854281059846329 1105 26-27 -0.08099170346042683 1105 28-29 -0.07000116851766336 1105 30-31 -0.34591050804537815 1105 32-33 0.07486472079153828 1105 34-35 0.26141086988570095 1105 36-37 0.13155020515441151 1105 38-39 0.07614798756651453 1105 40-41 0.2408979200673187 1105 42-43 0.1600590089588323 1105 44-45 0.07845370414232633 1105 46-47 0.26952837152831677 1105 48-49 0.5378083397534894 1105 50-51 0.35351148389274556 1105 52-53 0.4903790928320646 1105 54-55 0.743308112105673 1105 56-57 0.8675109574031197 1105 58-59 0.9415541528077682 1105 60-61 0.8460480563932755 1105 62-63 0.8510716496191684 1105 64-65 0.9988503351976519 1105 66-67 1.1227020363609448 1105 68-69 1.2536754941688066 1105 70-71 0.8993152767687 1105 72-73 1.364724459908654 1105 74-75 1.064420639951809 1105 76-77 1.051102960355383 1105 78-79 0.5733095921832607 1105 80-81 0.9247497055310205 1105 82-83 1.153150067026715 1105 84-85 0.868586751674929 1105 86-87 0.6270585610803714 1105 88-89 0.6215712811234475 1105 90-91 0.972167212156279 1105 92-93 1.085436138953316 1105 94-95 1.2644669538854565 1105 96-97 1.0588814311681958 1105 98-99 1.3822263510010693 1105 100-101 1.5981697304095341 1106 1 -0.014619959289621676 1106 2 -0.02380480158108611 1106 3 -0.036277379496944206 1106 4 0.07283232399288408 1106 5 0.1471223697307309 1106 6 0.13924800781151703 1106 7 -0.2522230939940897 1106 8 -0.12594268763563576 1106 9 -0.2823924513747116 1106 10-11 -0.4373939789869823 1106 12-13 0.07103212663382408 1106 14-15 -0.16382934923970538 1106 16-17 -0.2211117280738506 1106 18-19 -0.17823446272882393 1106 20-21 -0.19970410867311017 1106 22-23 0.018791642334186065 1106 24-25 0.06766303873750701 1106 26-27 -0.12736800487704159 1106 28-29 0.09439690851482396 1106 30-31 -0.07873251976523932 1106 32-33 0.06607838068799055 1106 34-35 0.5593428264198081 1106 36-37 0.40279600933033066 1106 38-39 0.37329411602985374 1106 40-41 0.2607658582820349 1106 42-43 0.5041361410063914 1106 44-45 0.9470586281908098 1106 46-47 1.0582135140441054 1106 48-49 1.0645407172923171 1106 50-51 1.0065523896218238 1106 52-53 1.0678684965983507 1106 54-55 0.98637255711904 1106 56-57 1.1052523950773505 1106 58-59 1.0820893692838425 1106 60-61 1.4932805903865578 1106 62-63 1.5668827948657942 1106 64-65 1.5093114491533868 1106 66-67 1.6121715846342575 1106 68-69 1.21899238556097 1106 70-71 1.4558948978644501 1106 72-73 1.164005836138955 1106 74-75 1.3727052590563211 1106 76-77 1.3439003660560118 1106 78-79 1.4811614724813786 1106 80-81 1.2238284021231607 1106 82-83 1.6095454985565532 1106 84-85 1.2597584533305017 1106 86-87 1.4212889473806527 1106 88-89 1.7461979238952487 1106 90-91 1.890981571830853 1106 92-93 1.9771892111499376 1106 94-95 1.8236049609401306 1106 96-97 1.992555408524968 1106 98-99 2.2563054031715204 1106 100-101 2.1794211527227816 1107 1 0.0635751079749518 1107 2 0.09322109166352277 1107 3 -0.05180066895159641 1107 4 -0.10161256245294226 1107 5 0.2555232087297199 1107 6 0.1257589292638528 1107 7 0.09593436297105029 1107 8 -0.07428277153553609 1107 9 -0.09196318766864664 1107 10-11 0.0042512907945919665 1107 12-13 -0.07419697071899378 1107 14-15 0.1995214029662833 1107 16-17 0.033214360455005476 1107 18-19 0.30191163881899286 1107 20-21 -0.027438088132107907 1107 22-23 -0.18305632526308813 1107 24-25 0.05736993154815195 1107 26-27 0.02985841635830866 1107 28-29 -0.06973226839824065 1107 30-31 -0.12027562695458727 1107 32-33 -0.17416644789583557 1107 34-35 0.14139837393897636 1107 36-37 0.3517418360336393 1107 38-39 0.1595030701742175 1107 40-41 0.06299083296740804 1107 42-43 0.3451829007344358 1107 44-45 0.22429445200101839 1107 46-47 0.2881833173133046 1107 48-49 0.4413127410157358 1107 50-51 0.5546863401498072 1107 52-53 0.7355191186148389 1107 54-55 0.5617965770814379 1107 56-57 0.8469302122334383 1107 58-59 0.9783084492780603 1107 60-61 1.008519090314227 1107 62-63 0.982178252698862 1107 64-65 1.0608990377545737 1107 66-67 1.0017048914475097 1107 68-69 0.9468782527676822 1107 70-71 0.9440693517918355 1107 72-73 0.78147021484574 1107 74-75 1.0287201946848477 1107 76-77 1.2018491581867785 1107 78-79 1.2215328741883056 1107 80-81 1.5067148117861109 1107 82-83 1.439530671419277 1107 84-85 1.1757319814658977 1107 86-87 1.363038187941914 1107 88-89 1.3160572469098604 1107 90-91 1.3129763642509324 1107 92-93 1.4444474564848129 1107 94-95 1.5409536156486503 1107 96-97 1.5914361404792565 1107 98-99 1.510762585292163 1107 100-101 1.8494766640781997 1108 1 -0.007541646036610672 1108 2 0.03810736320709651 1108 3 0.03423855520962249 1108 4 -0.25425181903082006 1108 5 -0.18728299365830736 1108 6 -0.21801977298910913 1108 7 -0.3483900197616734 1108 8 -0.2592792597304694 1108 9 0.08958092600578027 1108 10-11 0.027829009742127653 1108 12-13 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#Quality Count 2 1772.0 3 367.0 4 237.0 5 230.0 6 328.0 7 365.0 8 423.0 9 613.0 10 785.0 11 1148.0 12 1920.0 13 2933.0 14 3218.0 15 4919.0 16 7103.0 17 10296.0 18 7978.0 19 4332.0 20 3836.0 21 3900.0 22 3965.0 23 4213.0 24 4407.0 25 4744.0 26 5293.0 27 5825.0 28 6761.0 29 8018.0 30 9796.0 31 12042.0 32 15194.0 33 19986.0 34 27756.0 35 41076.0 36 55572.0 37 75150.0 38 63736.0 39 9335.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.387495215244 19.550279616472633 15.877921035187795 19.184304133095573 2 15.018141515487372 21.786851776235196 41.489587586770114 21.705419121507322 3 17.973346896303934 29.096771484165384 31.560270450611032 21.369611168919644 4 11.34321532650009 17.634294700542007 38.61466993007319 32.40782004288471 5 11.402931582410506 39.69979513456253 33.258433782730634 15.638839500296331 6 27.336421878099728 38.44841468964793 18.16040983510905 16.05475359714329 7 24.938326926802173 37.15297956211535 20.813874516009324 17.094818995073155 8 26.838044832752274 33.150847323149584 19.994586092792503 20.01652175130564 9 28.442420013860918 15.391220950989748 20.71437405347888 35.451984981670456 10-11 24.09324066986539 28.385499318559454 26.227643149188808 21.29361686238635 12-13 26.591160240335416 26.775764350869167 27.72130613826171 18.911769270533707 14-15 22.974945311360653 26.496135336152836 29.17532448592679 21.353594866559718 16-17 18.97851614235754 30.352089748870732 29.21914328289588 21.450250825875848 18-19 18.952278611423218 26.396516540482267 35.625770786590266 19.02543406150425 20-21 20.72624818437954 25.14218714453214 35.852043703224076 18.279520967864247 22-23 19.983130593240805 25.769710431849813 35.763492317186845 18.483666657722537 24-25 19.209520009892373 25.861324784829204 36.09678194548462 18.8323732597938 26-27 18.71003419515388 26.353951926117002 36.09274575068921 18.84326812803991 28-29 18.60302568859795 26.61054031820489 36.02518101039096 18.7612529828062 30-31 18.86977170228531 26.487785809718844 35.89285818470353 18.74958430329232 32-33 18.43246346506602 26.631061345083012 35.76852884754512 19.167946342305843 34-35 18.849130406370485 26.964520766624034 35.41446895508733 18.77187987191815 36-37 18.895668303837045 26.90924787381733 35.10902154765099 19.086062274694633 38-39 18.837658049899318 27.220398382577006 34.9708224544604 18.97112111306328 40-41 18.947726989037704 27.11572763298651 35.006550312583485 18.9299950653923 42-43 18.611298425239106 27.587365512993117 34.37769061513441 19.423645446633362 44-45 18.686426091776305 27.79847720178535 34.013360949852675 19.501735756585663 46-47 19.13039926716417 27.987910542683604 33.01402073645333 19.8676694536989 48-49 19.186692434087686 28.373338188064828 32.261791395922025 20.17817798192546 50-51 19.282597558399665 28.88538529134774 31.77207995826803 20.059937191984567 52-53 19.400741194665734 29.11634421780618 31.30806268055677 20.174851906971313 54-55 19.046791030406293 28.95850716832284 31.351449127372668 20.643252673898203 56-57 19.368691464490205 28.95789876477533 30.893099634612426 20.780310136122036 58-59 19.473098563950398 28.55594901510698 31.31012699567972 20.660825425262903 60-61 19.606198834365347 28.51897684476205 31.66224820297019 20.21257611790242 62-63 19.673389401486077 28.35633216297869 31.787726440294417 20.182551995240814 64-65 19.473736389985596 28.446344322429773 31.756253171053167 20.323666116531466 66-67 19.518126532316572 28.480268544675415 31.50069632197368 20.500908601034336 68-69 19.610226310423904 28.26757399238089 31.588628723530825 20.533570973664382 70-71 19.47707478208533 28.435458416877495 31.432783837281047 20.654682963756123 72-73 19.4360913657031 28.445751905396232 31.264011981775386 20.854144747125282 74-75 19.173826926621235 28.676112066330877 31.110561568126094 21.039499438921798 76-77 18.897180298490746 28.375638189641496 31.49495048488412 21.23223102698364 78-79 18.852732494791194 28.408123172834408 31.47297836449285 21.266165967881552 80-81 18.977311169437154 28.707028288130648 31.105045202682994 21.210615339749197 82-83 18.92361637648464 28.439316067833303 30.947370631230477 21.689696924451578 84-85 18.944344259809217 28.18095289196623 30.864125528107817 22.010577320116738 86-87 18.88405808935981 28.346151737396337 30.59991997489592 22.169870198347937 88-89 18.90222492338812 27.85627879142787 30.659334634403546 22.582161650780467 90-91 18.83505588824451 28.022754291220863 30.889556988976345 22.25263283155828 92-93 19.091697025478073 28.03182746629618 30.565519622699178 22.310955885526575 94-95 19.02463434577668 28.13650779949586 30.225743876990407 22.613113977737058 96-97 19.083312236853004 28.07414605919692 30.05822628570895 22.784315418241125 98-99 19.317504337204053 28.01711314298248 29.843813986155947 22.821568533657523 100-101 19.815623053649865 27.471091962200862 29.87284428965656 22.84044069449272 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1151.0 1 789.0 2 549.0 3 667.5 4 542.5 5 551.5 6 1011.0 7 2201.5 8 3663.0 9 4066.5 10 3187.0 11 2359.5 12 2284.0 13 2280.5 14 2268.5 15 2219.5 16 2100.5 17 2112.0 18 2190.0 19 2264.5 20 2298.0 21 2530.0 22 2810.5 23 3116.0 24 3511.5 25 3961.0 26 4539.0 27 5238.5 28 5763.5 29 6215.5 30 7036.0 31 7847.0 32 8567.0 33 9074.0 34 9638.0 35 10428.0 36 11219.0 37 12035.0 38 12362.0 39 12027.0 40 11837.0 41 12003.5 42 11938.0 43 12233.0 44 12698.0 45 12995.0 46 13122.0 47 13345.5 48 14387.0 49 14267.0 50 13362.0 51 12344.5 52 11140.5 53 10824.0 54 10556.5 55 9706.5 56 8687.0 57 7785.0 58 7210.5 59 6477.5 60 5327.0 61 4578.5 62 3932.5 63 2902.0 64 1940.5 65 1454.0 66 1105.5 67 819.0 68 597.0 69 418.5 70 319.0 71 236.5 72 187.5 73 168.0 74 146.0 75 113.5 76 81.0 77 65.0 78 49.0 79 31.0 80 20.0 81 13.5 82 12.5 83 9.5 84 6.5 85 4.0 86 3.0 87 3.0 88 3.0 89 3.5 90 1.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2628191250381193 2 0.2323237261187272 3 0.22231378601541532 4 0.22859909724307625 5 0.2325565154234554 6 0.2579305496388274 7 0.25699939241991465 8 0.2437304020504082 9 0.23977298387002904 10-11 0.24885176675442824 12-13 0.25339115819662783 14-15 0.2352335924278295 16-17 0.25339115819662783 18-19 0.2405877464365777 20-21 0.23290569938054767 22-23 0.2296466491143531 24-25 0.22347773253905626 26-27 0.2336040672947322 28-29 0.2503648972351614 30-31 0.2503648972351614 32-33 0.2511796598017101 34-35 0.2553698672868174 36-37 0.22999583307144536 38-39 0.23046141168090173 40-41 0.22522365232451758 42-43 0.23278930472818357 44-45 0.2526927902824433 46-47 0.256184629853366 48-49 0.26188796781920654 50-51 0.2617715731668424 52-53 0.2817914533734662 54-55 0.2620043624715706 56-57 0.2297630437667172 58-59 0.2088120063411807 60-61 0.20473819350843744 62-63 0.21602847478775436 64-65 0.20729887586044748 66-67 0.20764805981753975 68-69 0.21276942452155978 70-71 0.21125629404082658 72-73 0.21463173895938525 74-75 0.21742521061612347 76-77 0.2367467229085627 78-79 0.2262712041957944 80-81 0.22068426088231805 82-83 0.21742521061612347 84-85 0.21416616034992889 86-87 0.2228957592772358 88-89 0.22149902344886668 90-91 0.23150896355217856 92-93 0.22103344483941031 94-95 0.21498092291647752 96-97 0.2296466491143531 98-99 0.23593196034201405 100-101 0.20625132398917065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 429573.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.02718740270954 #Duplication Level Percentage of deduplicated Percentage of total 1 77.93393299886675 45.22286935155449 2 12.474228915760797 14.476888379982999 3 4.19865117227443 7.309077552365231 4 1.912325792395355 4.438675485214411 5 1.0183087769857586 2.954479711798828 6 0.6082436916366297 2.1176802408668745 7 0.3687668065878943 1.4978950415642136 8 0.2711052518165416 1.2585180202413773 9 0.19767873233493424 1.0323666762056356 >10 0.9399153207166151 9.662148726663283 >50 0.049227252590885354 1.954973742847322 >100 0.02321285081072364 2.2661717486149824 >500 0.0020011078285106587 0.7046080226786079 >1k 0.0016008862628085268 1.870903833453812 >5k 8.004431314042634E-4 3.2327434659479968 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8025 1.8681341704436731 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5895 1.372292951372642 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4321 1.0058825857304812 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1448 0.3370789132464098 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1204 0.280278322892733 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1083 0.2521108170206228 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 666 0.15503767694897028 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 641 0.14921794433076566 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 628 0.14619168336929927 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 552 0.12849969620995733 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.1273357496863164 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1639465236409178E-4 0.0 12-13 0.0 0.0 0.0 2.3278930472818356E-4 0.0 14-15 0.0 0.0 0.0 2.3278930472818356E-4 0.0 16-17 0.0 0.0 0.0 3.491839570922753E-4 0.0 18-19 0.0 0.0 0.0 4.655786094563671E-4 0.0 20-21 0.0 0.0 0.0 4.655786094563671E-4 0.0 22-23 0.0 0.0 0.0 8.147625665486425E-4 0.0 24-25 0.0 0.0 0.0 0.0018623144378254685 0.0 26-27 0.0 0.0 0.0 0.0033754449185586614 0.0 28-29 0.0 0.0 0.0 0.006168916575296864 0.0 30-31 0.0 0.0 0.0 0.01175585988877327 0.0 32-33 0.0 0.0 0.0 0.017226408549885584 0.0 34-35 0.0 0.0 0.0 0.02397729838700291 0.0 36-37 0.0 0.0 0.0 0.032357713357217516 0.0 38-39 0.0 0.0 0.0 0.043997178593626694 0.0 40-41 0.0 0.0 0.0 0.06436624275734276 0.0 42-43 0.0 0.0 0.0 0.10091416359966758 0.0 44-45 0.0 0.0 0.0 0.14979991759258612 0.0 46-47 0.0 0.0 0.0 0.20485458816080154 0.0 48-49 0.0 0.0 0.0 0.3046048052368282 0.0 50-51 0.0 0.0 0.0 0.4166928554634486 0.0 52-53 0.0 0.0 0.0 0.5335530864369968 0.0 54-55 0.0 0.0 0.0 0.6846333452055879 0.0 56-57 0.0 0.0 0.0 0.8610876381895509 0.0 58-59 0.0 0.0 0.0 1.0760685611060286 0.0 60-61 0.0 0.0 0.0 1.3221268562037185 0.0 62-63 0.0 0.0 0.0 1.6285939758783723 0.0 64-65 0.0 0.0 0.0 1.905962432462003 0.0 66-67 0.0 0.0 0.0 2.250141419502622 0.0 68-69 0.0 0.0 0.0 2.6856902086490537 0.0 70-71 0.0 0.0 0.0 3.1497556876246877 0.0 72-73 0.0 0.0 0.0 3.6564216093655793 0.0 74-75 0.0 0.0 0.0 4.210809338575748 0.0 76-77 0.0 0.0 0.0 4.770784011099394 0.0 78-79 2.3278930472818356E-4 0.0 0.0 5.354735982010043 0.0 80-81 2.3278930472818356E-4 0.0 0.0 6.0568285250702445 0.0 82-83 2.3278930472818356E-4 0.0 0.0 6.868215646700328 0.0 84-85 2.3278930472818356E-4 0.0 0.0 7.676343718064217 0.0 86-87 2.3278930472818356E-4 0.0 0.0 8.482609474990282 0.0 88-89 2.3278930472818356E-4 0.0 0.0 9.426104527053608 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2505 0.0 53.48626 1 GTATCAA 4960 0.0 46.458042 1 TATCAAC 6630 0.0 34.536926 2 ATCAACG 6650 0.0 34.433056 3 TCAACGC 6760 0.0 33.876705 4 CAACGCA 6845 0.0 33.24392 5 AACGCAG 6915 0.0 32.976093 6 CGCAGAG 7695 0.0 29.50657 8 ACGCAGA 7685 0.0 29.483158 7 GAGCGCA 65 0.0058396524 29.231161 9 CCTATAC 115 3.8532253E-6 28.916807 3 GTACATG 4155 0.0 28.587082 1 TACATGG 4080 0.0 28.410559 2 GCAGAGT 7945 0.0 27.920454 9 CTATACT 120 5.3610456E-6 27.715174 4 ACATGGG 4210 0.0 26.630545 3 CATGGGA 3050 0.0 26.481947 4 ATGGGAG 925 0.0 26.192598 5 ATTGGAC 130 9.991856E-6 25.580252 3 AGTACTT 5245 0.0 25.445421 12-13 >>END_MODULE