FastQCFastQC Report
Thu 26 May 2016
SRR937180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429573
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77731.8094712656521708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65681.5289601534547097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48421.127165813493865No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21910.5100413666594502No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13120.30541956780337687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12620.2937801025669677No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.19484464805748963No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.15387373042532934No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.14200147588419196No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.12919806412414186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33500.044.697481
GTATCAA57000.037.7781451
ATAACGC550.002578718134.543043
ACGCAGT600.003945071731.6718278
ATCAACG73550.029.3827193
ACCTAAT650.00581881629.2525651
GTAGACT650.005842044529.228734
TATCAAC74100.029.0364342
TCAACGC74500.029.008044
CAACGCA75450.028.6427975
AACGCAG76950.028.0844576
TCTAGAC1205.374308E-627.7063983
ACGCAGG1056.888287E-527.1472828
GACGTGA1056.888287E-527.1472827
TGAACCG700.0083886827.1409635
GTATACA1309.944333E-625.5959931
ACGCAGA85550.025.211597
CGCAGAG85950.024.9837118
GTACATG44550.024.9680981
CATGGGT3100.024.5144164