FastQCFastQC Report
Thu 26 May 2016
SRR937179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117871
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40230.35988052288680894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.29028394152813697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20400.18248975060628642No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18700.16728227138909588No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13010.11638194389155816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34400.037.5912931
GTATCAA53600.036.4547231
TGTACCG1553.6379788E-1133.7288365
GTACCGT902.410184E-531.6718856
ACCGTAT600.003947718531.6718838
TATCAAC64200.029.9792962
ATCAACG64750.029.7953683
TCAACGC64850.029.7520924
CAACGCA65600.029.2696785
AACGCAG66350.028.9987476
CCGTATG700.00838472727.1473279
GTACATG44700.026.5894471
ACGCAGA74400.025.5418427
CGCAGAG74650.025.329028
TACATGG46950.025.0013122
GAGTACT36900.024.71453312-13
ACATGGG46550.023.988923
GCAGAGT80700.022.7236949
GTACTTT39550.022.63670514-15
AGAGTAC61200.021.85106710-11
Produced by FastQC (version 0.11.5)