FastQCFastQC Report
Thu 26 May 2016
SRR937179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117871
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45820.40988629278333544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38530.34467304366961843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24880.22256593113158854No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17710.15842615113908493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47050.030.5997981
GTATCAA64700.025.8507981
GTACATG45200.023.232151
TACATGG44600.022.5777722
TAAGGTG5550.022.251565
ACATGGG42600.022.1882783
GTCTAGG2154.405956E-822.1002461
TACGGTG1100.002877835921.5902395
GAGTACT47050.021.45641512-13
GTACTTT47150.021.10485814-15
TATCAAC78350.020.9757372
TCAACGC78950.020.8163244
ATCAACG78750.020.8088763
CTACGGT1150.003723885920.6515334
GTATAGG3700.020.5472561
CAACGCA80050.020.5302815
AGTACTT47850.020.25377812-13
AACGCAG82650.020.0568476
AGAGTAC69550.019.84297610-11
GTATAAG5900.018.5231
Produced by FastQC (version 0.11.5)