FastQCFastQC Report
Thu 26 May 2016
SRR937178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082821
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40560.37457714617651483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30870.28508867116540965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20470.18904324906886733No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15560.14369872767521133No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12780.11802504753786636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG301.2983738E-463.3610732
GTATCAA53350.031.5437681
GGTATCA36850.029.67121
TTACGTA700.00836966227.157264
TATCAAC64950.025.5346572
GTATAGA3000.025.3538271
ATCAACG65650.025.261223
TCAACGC66200.025.054834
CAACGCA66150.025.0030825
AACGCAG67850.024.574246
GTACACG1601.8463506E-623.7692091
GTGTAAG4650.023.5136281
GTATAGG2903.6379788E-1222.9495831
GTATACG1050.002189375222.631054
GTACATG48000.022.3826751
TACATGG47900.021.9250472
ACGCAGA77550.021.1329527
CGCAGAG77650.021.1057328
GAGTACT35950.021.07485212-13
AGAGTAC61050.020.65768210-11
Produced by FastQC (version 0.11.5)