##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937178_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1082821 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.694504447180098 33.0 31.0 34.0 30.0 34.0 2 31.818077964871385 33.0 31.0 34.0 30.0 34.0 3 31.856099022830183 33.0 31.0 34.0 30.0 34.0 4 35.463348051062916 37.0 35.0 37.0 33.0 37.0 5 35.44985274574468 37.0 35.0 37.0 33.0 37.0 6 35.484204683876655 37.0 35.0 37.0 33.0 37.0 7 35.42293139863376 37.0 35.0 37.0 33.0 37.0 8 35.41407120844535 37.0 35.0 37.0 33.0 37.0 9 37.03011485739564 39.0 37.0 39.0 33.0 39.0 10-11 36.905996004879846 39.0 37.0 39.0 33.0 39.0 12-13 36.76981837256574 39.0 37.0 39.0 33.0 39.0 14-15 37.96015962010341 40.0 38.0 41.0 33.0 41.0 16-17 37.913931296123735 40.0 38.0 41.0 33.0 41.0 18-19 37.86928218052661 40.0 38.0 41.0 33.0 41.0 20-21 37.789537698289934 40.0 38.0 41.0 33.0 41.0 22-23 37.63729877791435 40.0 37.5 41.0 32.0 41.0 24-25 37.47322687683375 40.0 37.0 41.0 32.0 41.0 26-27 37.30559113648516 40.0 37.0 41.0 32.0 41.0 28-29 37.351941364269805 40.0 37.0 41.0 32.0 41.0 30-31 37.19298942299789 40.0 37.0 41.0 31.5 41.0 32-33 36.986862556230435 39.0 36.0 41.0 31.0 41.0 34-35 36.86407541043256 39.0 36.0 41.0 31.0 41.0 36-37 36.89229106195761 40.0 36.0 41.0 31.0 41.0 38-39 36.802162591970415 40.0 36.0 41.0 30.0 41.0 40-41 36.622222878943056 39.5 36.0 41.0 30.0 41.0 42-43 36.38597884599578 39.0 36.0 41.0 30.0 41.0 44-45 36.161475904142975 39.0 35.0 41.0 29.5 41.0 46-47 35.821877300126246 39.0 35.0 41.0 28.5 41.0 48-49 35.4990603248367 39.0 35.0 41.0 27.0 41.0 50-51 34.60991013288438 38.0 34.0 39.5 25.0 40.5 52-53 34.68225634707861 38.0 34.5 40.0 26.0 40.5 54-55 34.986555487933835 39.0 35.0 40.0 26.0 41.0 56-57 34.76128372094742 38.0 34.0 40.0 25.0 41.0 58-59 34.49676400808629 38.0 34.0 40.0 24.0 41.0 60-61 34.18417356146584 38.0 34.0 40.0 23.0 41.0 62-63 33.83996800948633 37.0 34.0 40.0 22.5 41.0 64-65 33.45244781916864 37.0 33.0 39.5 22.0 41.0 66-67 33.04973490539987 36.0 33.0 39.0 20.5 41.0 68-69 32.55957817589426 35.5 32.0 39.0 19.0 40.0 70-71 32.106647359074124 35.0 32.0 38.5 18.0 40.0 72-73 31.707268329668523 35.0 31.0 37.5 15.5 39.5 74-75 31.25553023075836 35.0 31.0 37.0 11.5 39.0 76-77 30.823164216430968 35.0 31.0 36.5 7.5 39.0 78-79 30.35977645428007 34.5 30.0 36.0 6.5 38.5 80-81 29.889792957469425 34.0 30.0 35.5 2.0 37.0 82-83 29.425896339284147 34.0 29.0 35.0 2.0 37.0 84-85 29.005213234689762 34.0 29.0 35.0 2.0 36.0 86-87 28.584536132934254 34.0 29.0 35.0 2.0 36.0 88-89 28.19844646529759 34.0 28.5 35.0 2.0 35.5 90-91 27.788572626500596 33.5 27.0 35.0 2.0 35.0 92-93 27.342589403049995 33.0 26.5 35.0 2.0 35.0 94-95 26.811001541344325 33.0 25.0 35.0 2.0 35.0 96-97 26.174780965644366 33.0 24.5 35.0 2.0 35.0 98-99 25.411475673264555 32.0 21.5 34.5 2.0 35.0 100-101 23.538755713086466 30.5 9.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0482826879753091 1101 2 -0.0028871354095727497 1101 3 -0.02413947798815741 1101 4 -0.1002120800370605 1101 5 -0.13805017191576496 1101 6 -0.0811706137941357 1101 7 -0.1628214479964356 1101 8 -0.03278653403375387 1101 9 0.041778564454141076 1101 10-11 -0.02775724529482204 1101 12-13 0.008043034431040041 1101 14-15 0.01568719921809958 1101 16-17 0.05916800328024863 1101 18-19 -0.027849113933712033 1101 20-21 0.025240878224778385 1101 22-23 0.01401508285418629 1101 24-25 -0.07393944649264483 1101 26-27 -0.08165550238169317 1101 28-29 -0.13311588490137183 1101 30-31 -0.05765380442923629 1101 32-33 0.06544929754108608 1101 34-35 -0.03230731408249454 1101 36-37 0.13079589205720765 1101 38-39 0.209166107719426 1101 40-41 0.018481978669051102 1101 42-43 0.3850671787824851 1101 44-45 0.3213432172839177 1101 46-47 0.4981132859096036 1101 48-49 0.5956433013136788 1101 50-51 0.5194533713730607 1101 52-53 0.5062412725085039 1101 54-55 0.7616420941610826 1101 56-57 0.6162532312721609 1101 58-59 0.678289611654769 1101 60-61 0.691546938218373 1101 62-63 0.5009801703527259 1101 64-65 0.4880725803315329 1101 66-67 0.5364983853908001 1101 68-69 0.6498068019579577 1101 70-71 0.5746952810625388 1101 72-73 0.7605297064135641 1101 74-75 0.507578665941022 1101 76-77 0.6999220626266442 1101 78-79 0.6367840475307247 1101 80-81 0.5748877798940661 1101 82-83 0.558201376497454 1101 84-85 0.579178634620682 1101 86-87 0.5620875202166467 1101 88-89 0.5661490971086032 1101 90-91 0.5880775290335549 1101 92-93 0.7020695613020074 1101 94-95 0.7324198201517405 1101 96-97 0.46146935402684264 1101 98-99 0.4429305562764725 1101 100-101 0.9868482407319696 1102 1 -0.1210298248929611 1102 2 -0.07082912884978754 1102 3 0.13047824247585282 1102 4 -0.09586532781344204 1102 5 -0.02748148587440369 1102 6 -0.224403024283383 1102 7 -0.024714974964425096 1102 8 -0.05173437917150636 1102 9 0.028018301175009697 1102 10-11 0.19081489776458227 1102 12-13 0.1717839208200047 1102 14-15 0.05494646365244904 1102 16-17 0.10358135611512864 1102 18-19 0.06716278549056653 1102 20-21 0.06518727225333265 1102 22-23 0.15985114737696904 1102 24-25 0.10064386817613524 1102 26-27 0.1101814637932037 1102 28-29 0.16128400890587358 1102 30-31 0.12025995176551163 1102 32-33 0.11681326211446219 1102 34-35 0.10432729612556813 1102 36-37 0.204630062863977 1102 38-39 0.39297800023953755 1102 40-41 0.3835458772007243 1102 42-43 0.3122117155885107 1102 44-45 0.2593560730183455 1102 46-47 0.5782877888810773 1102 48-49 0.3124577570079978 1102 50-51 0.2896921530166807 1102 52-53 0.45062939223507925 1102 54-55 0.49525814241795985 1102 56-57 0.6051736775283061 1102 58-59 0.6642149619285362 1102 60-61 0.5936638480585046 1102 62-63 0.29173605811224235 1102 64-65 0.24850332675071485 1102 66-67 0.413483078264008 1102 68-69 0.39096771398276786 1102 70-71 0.31112662913107414 1102 72-73 0.2424282389069461 1102 74-75 0.3451699150661831 1102 76-77 0.38169188160991396 1102 78-79 0.31643329910604834 1102 80-81 0.25648396714674604 1102 82-83 0.32139112084168175 1102 84-85 0.39070211024710133 1102 86-87 0.21256869586579086 1102 88-89 0.3241479718927991 1102 90-91 0.3048219155916243 1102 92-93 0.4121220371260996 1102 94-95 0.263623599693279 1102 96-97 0.36500737280043793 1102 98-99 0.4793434932171259 1102 100-101 0.7835111005143851 1103 1 -0.07537932437795547 1103 2 -0.07045142950353878 1103 3 -0.0750687836276498 1103 4 -0.1355406584693668 1103 5 -0.09866598313291064 1103 6 -0.10744423914023571 1103 7 0.005696582128592809 1103 8 -0.1519898965815898 1103 9 -0.1106100333736606 1103 10-11 -0.0756703690035394 1103 12-13 0.015914498328214677 1103 14-15 0.005713522861675813 1103 16-17 0.015343353139165572 1103 18-19 -0.13082011410815397 1103 20-21 -0.14477976566278272 1103 22-23 -0.06586878391048145 1103 24-25 -0.23509198225153938 1103 26-27 0.048783773420474574 1103 28-29 0.02695464493636024 1103 30-31 -0.1266985967874419 1103 32-33 -0.06430667136989143 1103 34-35 -0.088982102166419 1103 36-37 -0.030540801729536327 1103 38-39 0.030456759932334876 1103 40-41 0.09581966951339638 1103 42-43 0.07871916268737067 1103 44-45 0.06131972938460706 1103 46-47 0.07526640535231621 1103 48-49 0.26393500928327995 1103 50-51 0.1638293381510394 1103 52-53 0.337750731741167 1103 54-55 0.20413559799181513 1103 56-57 0.26635835222946724 1103 58-59 0.31657017506672247 1103 60-61 0.2408136544347812 1103 62-63 0.12974507557851922 1103 64-65 0.19486220365705975 1103 66-67 0.0812210901121091 1103 68-69 0.21732128450903332 1103 70-71 0.4352287482187478 1103 72-73 0.5111142169665825 1103 74-75 0.4867822434432334 1103 76-77 0.619523752168039 1103 78-79 0.28023922416948466 1103 80-81 0.27595238277826084 1103 82-83 0.19929494861753838 1103 84-85 0.3633793226400357 1103 86-87 0.567720151613706 1103 88-89 0.3867539621875302 1103 90-91 0.5683918419360268 1103 92-93 0.6908871799547533 1103 94-95 0.6882607506065597 1103 96-97 0.6615583947433343 1103 98-99 0.7610907287029498 1103 100-101 0.8899137381570661 1104 1 0.04063482112950467 1104 2 -0.07891740563643523 1104 3 0.011792613103608574 1104 4 -0.01841537509481128 1104 5 -0.023253950507431398 1104 6 0.047525952780453906 1104 7 0.05836812908706435 1104 8 -0.010425600227705445 1104 9 -0.045838951854669574 1104 10-11 0.073159754184843 1104 12-13 -0.01542655563592632 1104 14-15 0.15450451542083954 1104 16-17 0.2354604526221209 1104 18-19 0.2725603422495766 1104 20-21 0.3315010529327793 1104 22-23 0.3074348388117798 1104 24-25 0.13226894491216257 1104 26-27 -0.0976365910189756 1104 28-29 -0.11060499702268345 1104 30-31 0.09222351348008573 1104 32-33 -0.012153712750325951 1104 34-35 0.09930760553208273 1104 36-37 0.19324726678261328 1104 38-39 0.10087856231491799 1104 40-41 0.0163849222026613 1104 42-43 0.09583128785033779 1104 44-45 0.23207160507476488 1104 46-47 0.1944458317574913 1104 48-49 0.28653316266158413 1104 50-51 0.09627482187440961 1104 52-53 0.10599377508930274 1104 54-55 0.24410646072328035 1104 56-57 0.20528705020292648 1104 58-59 0.06008481078206529 1104 60-61 0.11951042387882893 1104 62-63 -0.05983551287734201 1104 64-65 0.052687727491480985 1104 66-67 0.20730255750210347 1104 68-69 0.20058455538242725 1104 70-71 0.2943838534328407 1104 72-73 0.2628720852331057 1104 74-75 0.09233346070680781 1104 76-77 0.303527150942692 1104 78-79 0.5033956125242085 1104 80-81 0.28589930272473296 1104 82-83 0.1621651415702452 1104 84-85 0.3364544396879161 1104 86-87 0.5307791176018775 1104 88-89 0.17382657831519666 1104 90-91 0.15505199543129677 1104 92-93 0.3727930050004211 1104 94-95 0.5790458744313511 1104 96-97 0.4941154222603359 1104 98-99 0.5915471724425672 1104 100-101 0.8650842788993636 1105 1 -0.10169395551182348 1105 2 -0.11044783772343081 1105 3 -0.07825208678005424 1105 4 -0.10625180452012017 1105 5 -0.2022294163464906 1105 6 -0.08015243253646531 1105 7 -0.03811565228436109 1105 8 -0.0219573328693059 1105 9 -0.2620757525616 1105 10-11 -0.15158743937865893 1105 12-13 -0.25404915250497595 1105 14-15 -0.2162626692703853 1105 16-17 -0.3744845143206348 1105 18-19 -0.25684933957775513 1105 20-21 -0.258546319323365 1105 22-23 -0.398913292932626 1105 24-25 -0.29752293071918245 1105 26-27 -0.3311803439919956 1105 28-29 -0.34136413787273767 1105 30-31 -0.4574828373240294 1105 32-33 -0.640404431173117 1105 34-35 -0.5024901885135193 1105 36-37 -0.3620764109720298 1105 38-39 -0.15621324615079857 1105 40-41 -0.41220505390632667 1105 42-43 -0.5178587864238011 1105 44-45 -0.5207354995165474 1105 46-47 -0.24899540900723593 1105 48-49 -0.2265100193145102 1105 50-51 -0.2327821958647931 1105 52-53 -0.48868815913827035 1105 54-55 -0.3524485839142457 1105 56-57 -0.3772367027344572 1105 58-59 -0.26187104518773907 1105 60-61 -0.24498692958162138 1105 62-63 -0.19236915359289952 1105 64-65 -0.3940204886286267 1105 66-67 -0.26693354482482334 1105 68-69 -0.2649828160185521 1105 70-71 -0.19239168865791711 1105 72-73 -0.1385316229509499 1105 74-75 -0.37082788467239425 1105 76-77 -0.2552115961421926 1105 78-79 -0.14758096145864386 1105 80-81 -0.068964147949508 1105 82-83 -0.01770579174693765 1105 84-85 0.04689419639512593 1105 86-87 -0.025165218660969657 1105 88-89 -0.0395454390944181 1105 90-91 -0.20953710948426618 1105 92-93 0.014589979518458307 1105 94-95 0.07410376692714848 1105 96-97 0.19855637084567235 1105 98-99 0.35322120891867215 1105 100-101 0.7912942846182389 1106 1 -0.06125127600383351 1106 2 -0.016415387328240882 1106 3 0.07195434370078146 1106 4 0.03638477915142602 1106 5 -0.05694640023018849 1106 6 0.11276004803700346 1106 7 0.09513980476726402 1106 8 0.06481930366282995 1106 9 0.06465511099496979 1106 10-11 -0.0653576868473138 1106 12-13 -0.3111749538115731 1106 14-15 -0.2649953810518255 1106 16-17 -0.2636286967630781 1106 18-19 -0.08336023423623118 1106 20-21 -0.09550172615887931 1106 22-23 0.07088436970946077 1106 24-25 -0.10401130834039662 1106 26-27 -0.03939415278870939 1106 28-29 -0.0036173291231804683 1106 30-31 -0.05820574505521847 1106 32-33 0.22180097456862313 1106 34-35 0.04372102343490525 1106 36-37 0.031297979981481205 1106 38-39 -0.1694624225434893 1106 40-41 -0.16499331160195396 1106 42-43 -0.20429470207046307 1106 44-45 -0.08232168673364981 1106 46-47 0.20641677381826895 1106 48-49 0.3281509150355859 1106 50-51 0.12483910665248033 1106 52-53 -0.015253596361084476 1106 54-55 -0.17645815268284082 1106 56-57 -0.35153996036445534 1106 58-59 -0.16865551795996936 1106 60-61 -0.14787029648147865 1106 62-63 -0.07572268517532876 1106 64-65 0.11547682045903684 1106 66-67 0.18500191411293798 1106 68-69 -0.047120272030845456 1106 70-71 0.130532133074432 1106 72-73 0.346203993878305 1106 74-75 0.1158626601650461 1106 76-77 0.0453147736320858 1106 78-79 0.1606982789705249 1106 80-81 0.0752297182967645 1106 82-83 -0.0932166178626197 1106 84-85 -0.286213820729877 1106 86-87 0.04614675362635623 1106 88-89 0.11214802974948057 1106 90-91 0.11501350985578895 1106 92-93 0.12613389867879476 1106 94-95 0.20143513342256014 1106 96-97 0.32061310191825854 1106 98-99 0.4758109695973687 1106 100-101 0.6411731680415294 1107 1 0.10205856933692914 1107 2 0.1667437117270012 1107 3 0.2525587255927384 1107 4 0.1788584026595501 1107 5 0.11130598836617622 1107 6 0.12497750145384856 1107 7 0.08955331873321626 1107 8 0.05401400546186608 1107 9 0.1488395887902456 1107 10-11 0.0892802416904388 1107 12-13 0.1350807294712837 1107 14-15 0.18200386614106634 1107 16-17 0.22108408113623312 1107 18-19 -0.005631103066008336 1107 20-21 -0.06562668392260207 1107 22-23 0.169966314765837 1107 24-25 0.21158458521493628 1107 26-27 0.28560926205936 1107 28-29 0.26865954778515544 1107 30-31 0.2762947987529216 1107 32-33 0.30752059346131233 1107 34-35 0.32193999273618346 1107 36-37 0.36752854230683596 1107 38-39 0.3284139527673062 1107 40-41 0.3969828430018225 1107 42-43 0.20585268585318772 1107 44-45 0.3631306086091115 1107 46-47 0.508070073793931 1107 48-49 0.6741094295099188 1107 50-51 0.7526124511187291 1107 52-53 0.6902095956848342 1107 54-55 0.6550647976637336 1107 56-57 0.7673450224175227 1107 58-59 0.7043971199973242 1107 60-61 0.8898375856040772 1107 62-63 0.7290337616983678 1107 64-65 0.6965732999304066 1107 66-67 0.8384890645275007 1107 68-69 0.770054667811344 1107 70-71 0.7765366581332458 1107 72-73 0.7378447025645443 1107 74-75 0.6837257047306124 1107 76-77 0.6723961620425563 1107 78-79 0.8870418547940169 1107 80-81 0.7687601987684545 1107 82-83 0.8045366688644577 1107 84-85 0.8275974167391702 1107 86-87 1.090768804404373 1107 88-89 0.9892137560748253 1107 90-91 1.0673907576217552 1107 92-93 1.0480966609160198 1107 94-95 0.9562030829423769 1107 96-97 0.9476834498424971 1107 98-99 1.0097553296548405 1107 100-101 1.1308523674573578 1108 1 0.07538957334429597 1108 2 0.04711317426361461 1108 3 -0.14847135808079415 1108 4 -0.00201232939544127 1108 5 -0.0300499794419693 1108 6 -0.058451559316907264 1108 7 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74-75 -1.5588023910211 2316 76-77 -1.5390335028960358 2316 78-79 -1.719636757641183 2316 80-81 -1.332802029407322 2316 82-83 -1.5014634703095417 2316 84-85 -1.6662371749591003 2316 86-87 -2.2486661491463025 2316 88-89 -2.257941875253241 2316 90-91 -2.5150072104378083 2316 92-93 -2.6824711690133505 2316 94-95 -2.4031215752114825 2316 96-97 -2.7101416084366186 2316 98-99 -2.6278668825159244 2316 100-101 -3.19599429758561 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5213.0 3 1285.0 4 1106.0 5 1226.0 6 1299.0 7 1455.0 8 1802.0 9 2128.0 10 2630.0 11 3252.0 12 4014.0 13 4826.0 14 5476.0 15 6572.0 16 8409.0 17 10529.0 18 9343.0 19 6877.0 20 6654.0 21 7050.0 22 7537.0 23 8398.0 24 9408.0 25 10611.0 26 12308.0 27 14243.0 28 16724.0 29 20475.0 30 24875.0 31 31246.0 32 39966.0 33 52850.0 34 72636.0 35 104621.0 36 157043.0 37 219670.0 38 168135.0 39 20928.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97850209650891 18.338545648296154 14.880070424166608 22.802881831028333 2 15.470505201695367 19.42994383354575 40.34740952606776 24.752141438691126 3 19.49427254675321 23.803106754393763 29.437850093358232 27.2647706054948 4 12.904988607524592 16.01087379360169 36.57679269399626 34.50734490487746 5 14.043659563589614 36.817379351738474 33.972505293499786 15.16645579117213 6 32.712600294508825 35.35244339590335 17.147873603697743 14.787082705890084 7 29.201239341313045 32.14643328453211 20.257936985342976 18.39439038881187 8 26.883808226379053 34.36443799489877 19.24686540974564 19.504888368976534 9 26.23647157353125 16.921695917526918 19.964556563744974 36.87727594519686 10-11 25.292316464507337 26.28709956902297 26.737376149642333 21.68320781682736 12-13 26.25583951317144 23.477862489443176 27.461017851151702 22.805280146233674 14-15 23.1847407863289 24.82107118976611 25.786756931221095 26.207431092683898 16-17 22.514635443153956 28.171822485760202 26.40117796171347 22.912364109372373 18-19 22.55636592807705 26.589642717336083 28.757844217836276 22.096147136750595 20-21 23.23388563205893 25.55627015597311 28.968060445941507 22.241783766026458 22-23 22.916749567604132 25.350289235587038 29.029656480107942 22.70330471670089 24-25 22.804817114357885 25.641325770543983 29.02917472449178 22.524682390606348 26-27 22.46882279596657 25.859625665356578 28.989722105915742 22.681829432761106 28-29 22.340886785215034 26.245866084984083 28.900363567038255 22.512883562762628 30-31 22.559703523252953 25.75521416631649 28.844655144008545 22.84042716642201 32-33 22.4412658895609 25.854762356490713 28.816664430058168 22.88730732389022 34-35 22.91594933709492 25.860193854845814 28.63709232540661 22.586764482652658 36-37 22.83204954046261 25.626936331029786 28.763792599282656 22.77722152922495 38-39 22.52935093656959 25.908681862451388 28.773934748964187 22.788032452014832 40-41 22.790283000710648 25.91098024991117 28.67815794148999 22.620578807888194 42-43 22.454704704936283 25.90046926286586 28.463925243677075 23.18090078852078 44-45 22.608568910111813 25.913746393760828 28.54369246501341 22.933992231113947 46-47 22.9476359285006 26.26592894944706 28.017274687991794 22.769160434060545 48-49 22.99459400425927 26.257303554851745 27.72458575782509 23.023516683063896 50-51 22.803827587339917 26.551168319342626 27.84876615055541 22.796237942762048 52-53 23.0949924484269 26.615279866436904 27.420148420927415 22.869579264208785 54-55 22.826159387829897 26.500161038941805 27.517482905808876 23.156196667419415 56-57 22.922752360640114 26.68053055993782 27.39785980966833 22.998857269753735 58-59 23.10438298327113 26.25357799981772 27.486764812699487 23.155274204211665 60-61 22.768366861649664 26.441091510004828 27.938993946953026 22.85154768139248 62-63 22.601093755783396 26.506042491764443 28.073388977310582 22.819474775141575 64-65 22.571356664890153 26.552692159649432 27.874576227275423 23.001374948184996 66-67 22.584856517655986 26.589177411072406 27.55911157990183 23.266854491369777 68-69 22.63698370314446 26.367200149515828 27.808047948690906 23.187768198648804 70-71 22.698978907287373 26.368713016040456 27.736986504180145 23.19532157249203 72-73 22.680336534124162 26.35459148856436 27.68990189433458 23.2751700829769 74-75 22.620148109489612 26.942259877281767 27.330253810770373 23.10733820245825 76-77 22.832045934572847 26.588566788582057 27.360265761670448 23.21912151517465 78-79 22.75322851083203 26.59870970607504 27.406346351014726 23.241715432078202 80-81 22.870879057324675 26.72881335543076 27.27611322245757 23.124194364786998 82-83 22.819675316564037 26.626632554395346 27.382870149757803 23.17082197928281 84-85 22.72817273067087 26.76442722269718 27.244764083034482 23.262635963597468 86-87 22.88893381402198 26.714072448407933 27.29160199683478 23.10539174073531 88-89 22.713745308458584 26.530634903819063 27.1526270087523 23.60299277897005 90-91 22.728462996873418 26.47184827334781 27.30059135790246 23.49909737187631 92-93 22.79238959866171 26.35475728730255 27.385259219233443 23.46759389480229 94-95 22.60822619467831 26.435193816452298 27.439307885290898 23.517272103578495 96-97 22.667490731582753 26.346268253485082 27.4089789151025 23.577262099829667 98-99 22.484938267949392 26.72077570580327 27.062909044811832 23.731376981435506 100-101 22.69726250966837 26.073929262687308 27.518957407359434 23.70985082028489 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1462.0 1 822.0 2 199.0 3 218.0 4 185.5 5 166.0 6 279.5 7 586.0 8 1007.0 9 1237.5 10 1203.0 11 1103.5 12 1119.0 13 1243.5 14 1312.5 15 1341.5 16 1431.5 17 1496.0 18 1525.5 19 1650.5 20 1795.0 21 2045.5 22 2423.0 23 2807.5 24 3333.0 25 4125.5 26 5281.0 27 6559.0 28 7577.5 29 8838.0 30 10587.5 31 12442.5 32 14431.5 33 15902.0 34 17225.5 35 18836.0 36 21120.0 37 23622.5 38 25551.0 39 27116.0 40 28591.5 41 30746.5 42 32290.0 43 34056.5 44 36676.5 45 38840.5 46 40382.0 47 42303.0 48 45052.0 49 45109.0 50 42882.0 51 41112.0 52 40514.0 53 40867.0 54 40853.0 55 38439.5 56 34375.0 57 31295.5 58 29172.5 59 26023.0 60 21557.0 61 17624.5 62 14072.5 63 10570.0 64 7643.5 65 5652.5 66 4158.0 67 3065.0 68 2252.0 69 1649.0 70 1231.0 71 858.0 72 644.0 73 504.0 74 366.5 75 265.0 76 182.0 77 121.0 78 85.5 79 59.5 80 36.0 81 25.5 82 20.0 83 17.5 84 12.0 85 10.5 86 12.0 87 8.5 88 7.5 89 7.5 90 5.0 91 5.0 92 6.0 93 5.0 94 4.5 95 3.0 96 1.5 97 5.0 98 7.0 99 3.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3367130855423011 2 0.29303088876185446 3 0.2871204012482211 4 0.2919226723530482 5 0.29506261884466595 6 0.21961155167843993 7 0.20751352254897165 8 0.21213109091899768 9 0.20557414383356068 10-11 0.22949314799029571 12-13 0.21781070001412975 14-15 0.21180786113309588 16-17 0.21337783437890473 18-19 0.20760587391637214 20-21 0.20188008913753983 22-23 0.20446592742475442 24-25 0.19310670923449028 26-27 0.19426110132699678 28-29 0.21033023925468752 30-31 0.2215509303938509 32-33 0.21388576689960762 34-35 0.22395206594626443 36-37 0.20031011589173095 38-39 0.19841691286002025 40-41 0.19550784478690386 42-43 0.20224949460714187 44-45 0.20580502225206196 46-47 0.2106072933568891 48-49 0.21739511886082744 50-51 0.22178180881235218 52-53 0.23849740631184654 54-55 0.21582514561501856 56-57 0.19172143872348246 58-59 0.1925064253463869 60-61 0.18867384359926526 62-63 0.19772427760451636 64-65 0.18959735727327046 66-67 0.18710387035345638 68-69 0.1846103834336423 70-71 0.1954154934195033 72-73 0.19379934448999417 74-75 0.1970778180327127 76-77 0.19887866969702286 78-79 0.19735487213491426 80-81 0.1987401426459221 82-83 0.19172143872348246 84-85 0.1862727080468517 86-87 0.1856724241587483 88-89 0.18650358646535298 90-91 0.19282965513228872 92-93 0.18811973539486213 94-95 0.18631888373055197 96-97 0.18470273480104285 98-99 0.19412257427589602 100-101 0.18257865335083084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1082821.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.993082260838364 #Duplication Level Percentage of deduplicated Percentage of total 1 80.97733574696731 50.20034636225272 2 10.836150877817806 13.4353278551883 3 3.303535380787375 6.143890218382252 4 1.4896409141500067 3.6938972692004732 5 0.8445082466176267 2.6176834601261456 6 0.5550958221405339 2.0647260578763493 7 0.37324407929326614 1.6196985637698824 8 0.2673437729302818 1.325877160575188 9 0.20119768877354932 1.1225578383746277 >10 1.0813766491850423 11.763783075235885 >50 0.046070762282719065 1.9164244286139207 >100 0.023163693163822205 2.7086788833428677 >500 5.939403958967517E-4 0.2802937406863138 >1k 7.424254948709397E-4 1.106815086375119 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4056 0.37457714617651483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3087 0.28508867116540965 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2047 0.18904324906886733 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1556 0.14369872767521133 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1278 0.11802504753786636 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 9.235136740052142E-5 6 0.0 0.0 0.0 0.0 1.8470273480104284E-4 7 0.0 0.0 0.0 0.0 1.8470273480104284E-4 8 0.0 0.0 0.0 0.0 1.8470273480104284E-4 9 0.0 0.0 0.0 0.0 1.8470273480104284E-4 10-11 0.0 0.0 0.0 0.0 1.8470273480104284E-4 12-13 0.0 0.0 0.0 0.0 1.8470273480104284E-4 14-15 0.0 0.0 0.0 0.0 1.8470273480104284E-4 16-17 0.0 0.0 0.0 0.0 1.8470273480104284E-4 18-19 0.0 0.0 0.0 0.0 1.8470273480104284E-4 20-21 0.0 0.0 0.0 4.617568370026071E-5 1.8470273480104284E-4 22-23 0.0 0.0 0.0 9.235136740052142E-5 2.7705410220156426E-4 24-25 0.0 0.0 0.0 9.235136740052142E-5 2.7705410220156426E-4 26-27 0.0 0.0 0.0 9.235136740052142E-5 2.7705410220156426E-4 28-29 0.0 0.0 0.0 4.155811533023464E-4 2.7705410220156426E-4 30-31 0.0 0.0 0.0 7.84986622904432E-4 2.7705410220156426E-4 32-33 0.0 0.0 0.0 0.0012929191436072997 2.7705410220156426E-4 34-35 0.0 0.0 0.0 0.0019855543991112107 3.694054696020857E-4 36-37 0.0 0.0 0.0 0.0027243653383153816 3.694054696020857E-4 38-39 0.0 0.0 0.0 0.003694054696020857 3.694054696020857E-4 40-41 0.0 0.0 0.0 0.005541082044031285 3.694054696020857E-4 42-43 0.0 0.0 0.0 0.007988393280145103 3.694054696020857E-4 44-45 0.0 0.0 0.0 0.015237975621086034 3.694054696020857E-4 46-47 0.0 0.0 0.0 0.028305694108259814 3.694054696020857E-4 48-49 0.0 0.0 0.0 0.04026519618662734 3.694054696020857E-4 50-51 0.0 0.0 0.0 0.0573501991557238 3.694054696020857E-4 52-53 0.0 0.0 0.0 0.0782677838719419 5.541082044031285E-4 54-55 0.0 0.0 0.0 0.09881596311855792 6.002838881033893E-4 56-57 0.0 0.0 0.0 0.12665990038981512 6.4645957180365E-4 58-59 0.0 0.0 0.0 0.17532907100988993 6.4645957180365E-4 60-61 0.0 0.0 0.0 0.2341107163603218 6.4645957180365E-4 62-63 0.0 0.0 0.0 0.3120090947626616 6.4645957180365E-4 64-65 0.0 0.0 0.0 0.3826578908240605 6.4645957180365E-4 66-67 0.0 0.0 0.0 0.4784262588184012 6.4645957180365E-4 68-69 0.0 0.0 0.0 0.5961280765703657 6.4645957180365E-4 70-71 0.0 0.0 0.0 0.7021012706624641 6.4645957180365E-4 72-73 0.0 0.0 0.0 0.8536036888830194 6.4645957180365E-4 74-75 0.0 0.0 0.0 0.995039808056918 6.926352555039106E-4 76-77 0.0 0.0 0.0 1.1443257934598607 7.388109392041714E-4 78-79 0.0 0.0 0.0 1.3265812170247897 7.388109392041714E-4 80-81 0.0 0.0 0.0 1.502325869187982 7.388109392041714E-4 82-83 0.0 0.0 0.0 1.7270629217571511 7.388109392041714E-4 84-85 0.0 0.0 0.0 2.0096581060027465 7.388109392041714E-4 86-87 0.0 0.0 0.0 2.3203742816218007 7.849866229044321E-4 88-89 0.0 0.0 0.0 2.730599055614917 8.311623066046928E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTACG 30 1.2983738E-4 63.361073 2 GTATCAA 5335 0.0 31.543768 1 GGTATCA 3685 0.0 29.6712 1 TTACGTA 70 0.008369662 27.15726 4 TATCAAC 6495 0.0 25.534657 2 GTATAGA 300 0.0 25.353827 1 ATCAACG 6565 0.0 25.26122 3 TCAACGC 6620 0.0 25.05483 4 CAACGCA 6615 0.0 25.003082 5 AACGCAG 6785 0.0 24.57424 6 GTACACG 160 1.8463506E-6 23.769209 1 GTGTAAG 465 0.0 23.513628 1 GTATAGG 290 3.6379788E-12 22.949583 1 GTATACG 105 0.0021893752 22.63105 4 GTACATG 4800 0.0 22.382675 1 TACATGG 4790 0.0 21.925047 2 ACGCAGA 7755 0.0 21.132952 7 CGCAGAG 7765 0.0 21.105732 8 GAGTACT 3595 0.0 21.074852 12-13 AGAGTAC 6105 0.0 20.657682 10-11 >>END_MODULE