FastQCFastQC Report
Thu 26 May 2016
SRR937178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082821
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44900.4146576396283411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38280.35352103440919597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26330.2431611503655729No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18050.16669421815794114No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.10712758618460486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG650.005843979329.2307138
GGTATCA47400.027.4767861
GTATCAA64400.027.2354281
TTACGCT700.00838387927.1478214
TAAGGTG4550.026.1036725
ATACCGT1351.3431356E-524.6284436
GGACCGT1000.001654046923.748866
TCAACGC77400.022.4653454
ATCAACG77750.022.3642143
AGGTAAG4900.022.2999942
CAACGCA78150.022.2497485
TATCAAC78200.022.2355212
GTACATG44250.021.8060231
AACGCAG79700.021.7524036
ATCACCG1553.9006496E-521.4555344
ACATGGG43650.021.4414923
GAGTACT41950.021.28720512-13
TACATGG44900.021.1619972
GGTAAGG5200.021.0134583
TACCGTC1150.00372318320.6521347
Produced by FastQC (version 0.11.5)