##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937178_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1082821 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27098107628131 33.0 31.0 34.0 31.0 34.0 2 32.51003259079756 34.0 31.0 34.0 31.0 34.0 3 32.58475315864764 34.0 31.0 34.0 31.0 34.0 4 36.17640127038541 37.0 35.0 37.0 35.0 37.0 5 36.0367207507058 37.0 35.0 37.0 35.0 37.0 6 36.12810889334433 37.0 35.0 37.0 35.0 37.0 7 36.095773909076385 37.0 35.0 37.0 35.0 37.0 8 36.069368806109225 37.0 35.0 37.0 35.0 37.0 9 37.79054248116725 39.0 38.0 39.0 35.0 39.0 10-11 37.714125880454844 39.0 37.0 39.0 35.0 39.0 12-13 37.63143261905707 39.0 37.0 39.0 35.0 39.0 14-15 38.881480872646534 40.0 38.0 41.0 35.0 41.0 16-17 38.857851851783444 40.0 38.0 41.0 35.0 41.0 18-19 38.85039447886585 40.0 38.0 41.0 35.0 41.0 20-21 38.750363171752305 40.0 38.0 41.0 35.0 41.0 22-23 38.61700872073962 40.0 38.0 41.0 34.0 41.0 24-25 38.470597633403855 40.0 38.0 41.0 34.0 41.0 26-27 38.248285265985785 40.0 38.0 41.0 34.0 41.0 28-29 38.2409451793048 40.0 38.0 41.0 34.0 41.0 30-31 38.3911828455488 40.0 38.0 41.0 34.0 41.0 32-33 38.36164703122677 40.0 38.0 41.0 34.0 41.0 34-35 38.24831851247805 40.0 38.0 41.0 34.0 41.0 36-37 38.102872496931624 40.0 38.0 41.0 34.0 41.0 38-39 37.96114085338205 40.0 37.5 41.0 33.0 41.0 40-41 37.812435296323216 40.0 37.0 41.0 33.0 41.0 42-43 37.60721070241526 40.0 37.0 41.0 33.0 41.0 44-45 37.33852548112753 40.0 37.0 41.0 32.5 41.0 46-47 37.018424097796405 40.0 36.5 41.0 31.5 41.0 48-49 36.71102610680805 39.5 36.0 41.0 31.0 41.0 50-51 36.48875622101899 39.0 36.0 41.0 31.0 41.0 52-53 36.24821230840554 39.0 35.5 41.0 30.0 41.0 54-55 36.03666164583066 39.0 35.0 40.0 30.0 41.0 56-57 35.768096481320555 39.0 35.0 40.0 29.0 41.0 58-59 35.49611847202816 38.0 35.0 40.0 28.5 41.0 60-61 35.13264657778156 38.0 34.0 40.0 28.0 41.0 62-63 34.75461271992324 37.5 34.0 40.0 27.5 41.0 64-65 34.389634574874336 37.0 34.0 39.5 26.5 41.0 66-67 33.980110286002954 36.0 33.5 39.0 26.0 40.5 68-69 33.52495380122846 36.0 33.0 39.0 26.0 40.0 70-71 33.120361075376266 35.0 33.0 38.0 25.5 40.0 72-73 32.68362360907297 35.0 32.0 37.5 25.0 39.5 74-75 32.245484249012534 35.0 32.0 37.0 24.0 39.0 76-77 30.980439518627733 34.0 30.5 35.5 22.5 38.0 78-79 31.597527199786484 34.5 31.5 36.0 23.5 38.0 80-81 31.435265847263768 35.0 32.0 35.5 23.0 37.0 82-83 31.115067494996865 34.0 31.0 35.0 21.5 37.0 84-85 30.80270284746971 34.0 31.0 35.0 20.0 36.0 86-87 30.491472736491072 34.0 31.0 35.0 19.5 36.0 88-89 30.14641016382209 34.0 31.0 35.0 17.5 36.0 90-91 29.717743745272763 34.0 30.0 35.0 12.5 35.0 92-93 29.313215665377747 34.0 30.0 35.0 4.5 35.0 94-95 28.930158354889684 34.0 29.0 35.0 2.0 35.0 96-97 28.51700881309099 34.0 29.0 35.0 2.0 35.0 98-99 27.879978777655772 33.5 29.0 35.0 2.0 35.0 100-101 26.271310308906088 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0137535530545847 1101 2 0.04878847637436934 1101 3 0.08127140127308508 1101 4 0.045857808308618075 1101 5 -0.15821306989563766 1101 6 -0.02573609545703448 1101 7 -0.05325397745249205 1101 8 -0.0787435676807533 1101 9 -0.07620984780820095 1101 10-11 -0.02898656317825754 1101 12-13 -0.007359434593006142 1101 14-15 0.01236162220228465 1101 16-17 0.03221012935586742 1101 18-19 0.12235258056894338 1101 20-21 -0.045276470014904646 1101 22-23 -0.010389867053902435 1101 24-25 -0.06908689781221966 1101 26-27 -0.026428135033974343 1101 28-29 -0.01982611998761996 1101 30-31 0.07068513830658674 1101 32-33 -0.009607627626948556 1101 34-35 0.14725228195893436 1101 36-37 0.1371488593081196 1101 38-39 0.015939100891735336 1101 40-41 0.12402450149564714 1101 42-43 -0.14210934888529891 1101 44-45 0.118216949858855 1101 46-47 0.011497289093917118 1101 48-49 0.1805459441259245 1101 50-51 0.19071514547581359 1101 52-53 0.2608175864661604 1101 54-55 0.14496137039188994 1101 56-57 0.14841512444374416 1101 58-59 0.0795802785658708 1101 60-61 0.22242309001458693 1101 62-63 0.05901167972509569 1101 64-65 0.026597952751899356 1101 66-67 0.16597968282814435 1101 68-69 0.1541422458644348 1101 70-71 0.04943692995747284 1101 72-73 -0.10897278464230453 1101 74-75 -0.026541072456502945 1101 76-77 -0.1519726381863542 1101 78-79 0.07030473837642504 1101 80-81 -0.07284002984584959 1101 82-83 -0.005334824708125296 1101 84-85 -0.0435087384531343 1101 86-87 -0.08689912492914686 1101 88-89 0.008458129289930838 1101 90-91 0.20447378038119268 1101 92-93 0.19144462298939402 1101 94-95 -0.015418030050526 1101 96-97 -0.07541965974528253 1101 98-99 -0.28024051201241207 1101 100-101 0.11973168811637791 1102 1 0.10697427165570161 1102 2 0.12253244087376913 1102 3 -0.06607392367715903 1102 4 -0.05449352368305682 1102 5 -0.09392782234611019 1102 6 -0.1478914631843793 1102 7 -0.04325854324782341 1102 8 0.035040490337316044 1102 9 0.07231165232063574 1102 10-11 0.011253669163600932 1102 12-13 0.013278494549631148 1102 14-15 0.030610625309570594 1102 16-17 0.007715931943366172 1102 18-19 -0.0710474821439675 1102 20-21 -0.02719103005264145 1102 22-23 0.0738848105356027 1102 24-25 0.08311107441573995 1102 26-27 0.0265143371777512 1102 28-29 0.05292228742926852 1102 30-31 0.06679065050786193 1102 32-33 0.016042196830341027 1102 34-35 0.13323763278338419 1102 36-37 0.10127263962227318 1102 38-39 0.03409358258355866 1102 40-41 0.05234295318554416 1102 42-43 0.19276029991832644 1102 44-45 0.19069576576031722 1102 46-47 0.18657190964179904 1102 48-49 0.3299709456978661 1102 50-51 0.22176173179379077 1102 52-53 0.15506846520710127 1102 54-55 0.13487531891716742 1102 56-57 0.17026621540581033 1102 58-59 0.2182168934498705 1102 60-61 0.3261771929632076 1102 62-63 0.2807467970200008 1102 64-65 0.30475660160524853 1102 66-67 0.27112592184156625 1102 68-69 0.19166005595940305 1102 70-71 0.17272429437574743 1102 72-73 0.057682649044551226 1102 74-75 -0.005969719267369555 1102 76-77 0.044964785759862735 1102 78-79 0.061123626714167045 1102 80-81 0.1910224603989228 1102 82-83 0.06567336125741718 1102 84-85 -0.1631769600655808 1102 86-87 -0.1162749424880225 1102 88-89 -0.07173322315125219 1102 90-91 -0.1129449548318533 1102 92-93 -0.19267766963986332 1102 94-95 -0.1893970241043057 1102 96-97 -0.09402620038065379 1102 98-99 0.0484176414254307 1102 100-101 0.0875200226120505 1103 1 0.022345114111139708 1103 2 0.029554606840100917 1103 3 6.82112118937539E-4 1103 4 -0.032334739553867564 1103 5 -0.05917821504358045 1103 6 -0.0065414964337477954 1103 7 0.026797925761819386 1103 8 -0.05367534033508292 1103 9 -0.07388503355772258 1103 10-11 -0.036676969351084665 1103 12-13 0.07216748389546979 1103 14-15 0.020362678785723176 1103 16-17 -0.14138764470889953 1103 18-19 0.026747074760621103 1103 20-21 -0.08806251848380953 1103 22-23 -0.012037988393991839 1103 24-25 -0.11047842976725519 1103 26-27 -0.003550995514537192 1103 28-29 -0.15391838005926672 1103 30-31 -0.013349208095696952 1103 32-33 0.11141085927612693 1103 34-35 0.05706403979794317 1103 36-37 0.11737450353052026 1103 38-39 0.13368263386532675 1103 40-41 0.221204134733874 1103 42-43 0.32731442457262716 1103 44-45 0.19668149768165932 1103 46-47 8.566341113933618E-4 1103 48-49 -0.12828334820292753 1103 50-51 -0.1471575833451908 1103 52-53 0.07886635465357728 1103 54-55 -0.09118978056478255 1103 56-57 -0.10503034617819651 1103 58-59 -0.027226210410695728 1103 60-61 -0.20128691503683882 1103 62-63 -0.1524040097948074 1103 64-65 -0.11580084714128702 1103 66-67 -0.1454734996954059 1103 68-69 -0.37661607183648016 1103 70-71 -0.2341451441703768 1103 72-73 -0.19170686375709067 1103 74-75 -0.1299349540282222 1103 76-77 -0.4694509505299429 1103 78-79 -0.10506640928979039 1103 80-81 -0.24464169475016817 1103 82-83 -0.19389755707587497 1103 84-85 -0.27180206684802855 1103 86-87 -0.27584298794037565 1103 88-89 -0.2020855481991397 1103 90-91 -0.22814980391688522 1103 92-93 -0.21363111122447975 1103 94-95 -0.39766230254984336 1103 96-97 -0.16622465561567878 1103 98-99 -0.19398696836551466 1103 100-101 0.11894152409792014 1104 1 0.012341433251947365 1104 2 0.04015513729663667 1104 3 -0.013164110528322226 1104 4 0.027283999115454094 1104 5 -0.08408765558738907 1104 6 -0.011682659103648518 1104 7 -0.04035625407739474 1104 8 0.003859584826514606 1104 9 -0.12676298383516382 1104 10-11 -0.05904764907162274 1104 12-13 -0.06191469214591905 1104 14-15 -0.06679112264740894 1104 16-17 -0.035934899028745804 1104 18-19 -0.0555128179784532 1104 20-21 -0.1051135826649201 1104 22-23 -0.1500048469010764 1104 24-25 -0.24894624632975138 1104 26-27 -0.1152143310609759 1104 28-29 -0.11949245649041274 1104 30-31 -0.07523021314225531 1104 32-33 -0.113650029702967 1104 34-35 -0.12998853800750254 1104 36-37 -0.08137856241878438 1104 38-39 -0.11510904670490163 1104 40-41 0.01151126287249582 1104 42-43 0.03393317700216869 1104 44-45 0.08980284907298852 1104 46-47 0.06377395582192946 1104 48-49 0.09158950735259452 1104 50-51 -0.0129128646901151 1104 52-53 0.011478681059777784 1104 54-55 -0.07215079441788674 1104 56-57 0.011351455241175756 1104 58-59 0.06252483278608167 1104 60-61 -0.16550474965962536 1104 62-63 -0.12912128540860124 1104 64-65 0.09604592935487943 1104 66-67 -0.21448788944679364 1104 68-69 -0.08782987815448706 1104 70-71 -0.17979098015541695 1104 72-73 -0.12615015960528808 1104 74-75 -0.06400930394376303 1104 76-77 -0.15347928970563984 1104 78-79 -0.06704691793636997 1104 80-81 -0.1080714987014133 1104 82-83 -0.17215339742978486 1104 84-85 -0.16178694780491654 1104 86-87 -0.20653361344563592 1104 88-89 -0.21281594074384813 1104 90-91 -0.2545496484263836 1104 92-93 -0.25694398769553217 1104 94-95 -0.3130360817602096 1104 96-97 -0.32539644509805044 1104 98-99 -0.05286600914049089 1104 100-101 0.17663727184554645 1105 1 0.030606208353987086 1105 2 -0.0030535625228935714 1105 3 -0.04491456794941229 1105 4 0.014264645793957698 1105 5 -0.21396941201310682 1105 6 -0.0453696957810692 1105 7 0.010903858625461282 1105 8 0.02239248796726656 1105 9 -0.08685065546828241 1105 10-11 -0.08253334920647859 1105 12-13 -0.11690276445675352 1105 14-15 -0.1840837777207298 1105 16-17 -0.18704308735618014 1105 18-19 -0.1180844152911078 1105 20-21 -0.14425649045559652 1105 22-23 -0.2220822958186801 1105 24-25 -0.2354732577799723 1105 26-27 -0.12784485418679026 1105 28-29 -0.22078183167191412 1105 30-31 -0.34103751905229984 1105 32-33 -0.22858810192472845 1105 34-35 -0.09113914579408089 1105 36-37 -0.26252688978085104 1105 38-39 -0.2559720270415866 1105 40-41 -0.10910582356449794 1105 42-43 -0.267688819473868 1105 44-45 -0.08390915647130015 1105 46-47 -0.0682490390524606 1105 48-49 -0.18943406475425206 1105 50-51 -0.04436312560275013 1105 52-53 -0.11778998857975154 1105 54-55 -0.0586575403768137 1105 56-57 0.14727834091173264 1105 58-59 0.12960539056366116 1105 60-61 0.15477976002238591 1105 62-63 -0.010393343616435402 1105 64-65 0.015021600075840524 1105 66-67 -0.1418786384059061 1105 68-69 -0.06414352738600115 1105 70-71 -0.0897514023190027 1105 72-73 0.016048784273110073 1105 74-75 0.05794463682577344 1105 76-77 -0.0349025596562349 1105 78-79 0.06202377344202148 1105 80-81 0.06020646010485464 1105 82-83 -0.0011186637138891342 1105 84-85 -0.09262527995943515 1105 86-87 -0.09149421376738687 1105 88-89 -0.12189069412841036 1105 90-91 -0.4114971933177891 1105 92-93 -0.3463531083246423 1105 94-95 -0.23772728912028285 1105 96-97 -0.20927495726358103 1105 98-99 -0.14425379889247836 1105 100-101 -0.19093776391970962 1106 1 -0.20663693238380887 1106 2 -0.06542945023715419 1106 3 -0.1565323772147451 1106 4 -0.04803783793441596 1106 5 -0.036243778612053745 1106 6 -0.11109834700956611 1106 7 -0.09222582482835406 1106 8 -0.01638523358273858 1106 9 -0.1009481097111049 1106 10-11 -0.22756261114641774 1106 12-13 -0.19200913947140208 1106 14-15 -0.05097755923643632 1106 16-17 -0.07252825124394491 1106 18-19 -0.051561445433904396 1106 20-21 -0.38944500501656165 1106 22-23 -0.16910041688080923 1106 24-25 -0.08778006310725317 1106 26-27 -0.0032044000348676605 1106 28-29 -0.2564299183800074 1106 30-31 -0.14446601992414543 1106 32-33 -0.2331369615135941 1106 34-35 -0.13285667267282264 1106 36-37 -0.33278544195285775 1106 38-39 -0.23836309139451828 1106 40-41 -0.3447780703924579 1106 42-43 -0.5436185344281981 1106 44-45 -0.3045809828062218 1106 46-47 -0.37887356113660786 1106 48-49 -0.6605627215245136 1106 50-51 -0.4308679243275577 1106 52-53 -0.3160283901423284 1106 54-55 -0.11112218857970646 1106 56-57 -0.13554600140556516 1106 58-59 -0.43117092221314834 1106 60-61 -0.4051251842464936 1106 62-63 -0.36374676339146816 1106 64-65 -0.40197161710419493 1106 66-67 -0.346127173607492 1106 68-69 -0.2808444897560065 1106 70-71 -0.448282719813605 1106 72-73 -0.37202545000421594 1106 74-75 -0.3760642076253511 1106 76-77 -0.35780426296899037 1106 78-79 -0.27493503622727644 1106 80-81 -0.2277486263781654 1106 82-83 -0.43752601986273376 1106 84-85 -0.6308724024988841 1106 86-87 -0.47722340944929087 1106 88-89 -0.4441363796997635 1106 90-91 -0.5875496047359476 1106 92-93 -0.5759235880610127 1106 94-95 -0.7591719476191763 1106 96-97 -0.6324530679033202 1106 98-99 -0.6193445694836299 1106 100-101 -0.39756998418610934 1107 1 -0.04009967048602192 1107 2 -0.007167007095659983 1107 3 0.027358474997818405 1107 4 -0.03342742331537352 1107 5 0.019571271162561743 1107 6 -0.023533426382954303 1107 7 0.023296688006851696 1107 8 -0.07676026092719468 1107 9 -0.021004719162377228 1107 10-11 0.017981397258729714 1107 12-13 -0.01280843986403113 1107 14-15 0.08283210688898635 1107 16-17 0.06071886359456613 1107 18-19 0.078332442655622 1107 20-21 -0.06659012014059584 1107 22-23 -0.06267295390238559 1107 24-25 0.0024130067538621347 1107 26-27 0.08591748590612269 1107 28-29 -0.22639260667164507 1107 30-31 -0.15311508548820996 1107 32-33 -0.18750278057902392 1107 34-35 -0.3130209756484206 1107 36-37 -0.17145389769179786 1107 38-39 -0.1589231983691306 1107 40-41 -0.17802949872326224 1107 42-43 -0.13734353286199052 1107 44-45 0.09727191889032127 1107 46-47 -0.04611946819728274 1107 48-49 0.07789817307325109 1107 50-51 0.0068675846491217385 1107 52-53 -0.07841222111915869 1107 54-55 -0.004174025459853681 1107 56-57 -0.032023726839462086 1107 58-59 -0.13531541804292857 1107 60-61 -0.11238848905941978 1107 62-63 -0.024009948042831297 1107 64-65 -0.04233111587639371 1107 66-67 -0.1979623078238575 1107 68-69 -0.23084880324829982 1107 70-71 -0.3804076077398335 1107 72-73 -0.24592769189048624 1107 74-75 -0.11204330362006942 1107 76-77 -0.3011706766801474 1107 78-79 -0.3941710037692765 1107 80-81 -0.26131093426086593 1107 82-83 -0.2024065412612508 1107 84-85 -0.2312114224528905 1107 86-87 -0.09452908918892078 1107 88-89 -0.09744457153514219 1107 90-91 0.0048536164157724215 1107 92-93 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-0.7404949607091496 2316 56-57 -0.4963951822515895 2316 58-59 -0.4339549921704773 2316 60-61 -0.4891951415767366 2316 62-63 -0.673218395904776 2316 64-65 -0.9456765109565879 2316 66-67 -0.4928198284390035 2316 68-69 -0.2824353868744822 2316 70-71 -0.47511725597754406 2316 72-73 -0.2330923695287055 2316 74-75 -0.39792236141207127 2316 76-77 -0.5387389589250233 2316 78-79 -0.5132500198090746 2316 80-81 -0.6943125695553931 2316 82-83 -0.8503067535016058 2316 84-85 -0.5780347758504796 2316 86-87 -0.5389777861723211 2316 88-89 -0.3182667419537104 2316 90-91 -0.5942626286696431 2316 92-93 -0.5684344310960086 2316 94-95 -0.4432278572927437 2316 96-97 -0.7665203912979379 2316 98-99 -1.1060660220113334 2316 100-101 -1.2466911276653612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 5.0 5 9.0 6 35.0 7 115.0 8 208.0 9 453.0 10 736.0 11 1289.0 12 1863.0 13 2732.0 14 3610.0 15 4838.0 16 6655.0 17 9387.0 18 8472.0 19 5238.0 20 4573.0 21 4587.0 22 4799.0 23 5355.0 24 5919.0 25 7246.0 26 8678.0 27 10978.0 28 13665.0 29 17119.0 30 22120.0 31 28191.0 32 36575.0 33 49083.0 34 68974.0 35 103129.0 36 166908.0 37 258271.0 38 202702.0 39 18298.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.26692623397646 19.10064916466016 15.250380203896466 22.382044397466906 2 16.18660886702419 20.334939939288212 38.96415012268879 24.51430107099881 3 19.908461324632604 23.90939961452539 29.571000193014356 26.611138867827645 4 13.685549135083269 16.670622383570322 35.903902861137716 33.739925620208695 5 14.162424170773699 37.15659797394652 33.70946230520848 14.971515550071304 6 33.06502182724568 35.672839739901605 16.51279389668283 14.749344536169875 7 29.349079857150905 32.400461387431534 19.779077058904473 18.471381696513088 8 27.225737217878116 34.58531003739307 18.87929768632119 19.30965505840762 9 26.551018127649904 16.770731265832488 19.580983375830353 37.09726723068725 10-11 25.634246103464932 26.53961273377594 26.307764625916935 21.518376536842194 12-13 26.501002307547022 23.571382722628414 27.20490343352099 22.722711536303574 14-15 23.198386436908777 25.137441922533828 25.581836702465132 26.08233493809226 16-17 22.570905071105933 28.403540382020665 26.243534249889873 22.782020296983525 18-19 22.67992586032225 26.718404981063355 28.737021169703947 21.86464798891045 20-21 23.272775463349898 25.683284679554607 29.08089148621979 21.963048370875704 22-23 23.020414043665156 25.473987320934405 29.08690687226781 22.41869176313263 24-25 23.025170504656845 25.793325729695283 28.992856582149308 22.188647183498567 26-27 22.61230747208042 26.04392788125203 29.041164206603725 22.302600440063816 28-29 22.48739025492857 26.386425481867043 28.956814018554784 22.169370244649603 30-31 22.67610752194634 25.92736593580692 28.894733755598274 22.501792786648462 32-33 22.654529234287107 25.96712660726011 28.850751878657693 22.52759227979509 34-35 23.021579744020478 25.954843875395838 28.744732508881892 22.27884387170179 36-37 23.102419193595978 25.727510448417302 28.799859995133076 22.370210362853644 38-39 22.710603259273487 26.016283438213993 28.839980495337166 22.43313280717535 40-41 22.92405670004553 25.98181047467679 28.721321437245862 22.372811388031817 42-43 22.721903000824813 26.046002979673382 28.506357898859207 22.725736120642598 44-45 22.892703410813052 26.085936641420883 28.419332465846153 22.60202748191991 46-47 23.299003251691648 26.47376620881937 27.869333897292346 22.357896642196632 48-49 23.23911338993241 26.56524023822959 27.555200721079476 22.64044565075853 50-51 23.01959418962137 26.85637792395973 27.698945624438387 22.42508226198051 52-53 23.327699894719345 26.858572985353057 27.309848358914685 22.50387876101291 54-55 23.11901043662803 26.75529935233986 27.345840171182495 22.779850039849613 56-57 23.23029383434566 27.03909510436166 27.21733324344467 22.51327781784801 58-59 23.335666744549652 26.585696066108806 27.268495900984558 22.810141288356984 60-61 22.95975050354583 26.674584257231803 27.814015428219435 22.551649811002928 62-63 22.866521798155006 26.776078410004978 27.92465236636526 22.432747425474755 64-65 22.84154075327316 26.915990731616766 27.619939029627243 22.622529485482826 66-67 22.914452157835875 26.817451822600415 27.393955233598167 22.874140785965547 68-69 22.890025221158435 26.737152308645655 27.596712660726013 22.776109809469894 70-71 22.997753091231147 26.775524301800573 27.44050032276803 22.78622228420025 72-73 23.008004093012605 26.699149720960342 27.461510258851646 22.831335927175406 74-75 22.865598284481 27.30700642119058 26.947898129053648 22.879497165274778 76-77 23.072326820407067 26.93386072120877 27.19817033470906 22.795642123675105 78-79 23.018070392059258 26.878542252135855 27.142898041319846 22.960489314485034 80-81 23.052332749364854 27.08744104519584 27.052116647165136 22.808109558274175 82-83 23.03030666840904 26.94261113053067 27.17265976767027 22.854422433390024 84-85 22.944107541369966 27.06293425364592 27.04598776990277 22.946970435081347 86-87 23.01590013492535 27.184594683701185 26.89872102591287 22.9007841554606 88-89 22.854146714923335 27.027458832069197 26.78582147926573 23.332572973741737 90-91 22.97624737640531 26.947301824798135 26.88883809101593 23.187612707780616 92-93 22.873577867607196 26.829043039620686 27.14212227083108 23.155256821941038 94-95 22.768029064822347 26.91488251520796 27.002339260136253 23.314749159833433 96-97 22.793379515173793 26.848204827944784 27.078159732772082 23.28025592410934 98-99 22.61617423306651 27.09593854835023 26.784108828710686 23.503778389872576 100-101 22.53563015298533 26.924023643061627 26.943512220307742 23.596833983645297 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 207.0 1 152.0 2 130.5 3 174.5 4 159.0 5 152.0 6 276.5 7 627.0 8 1053.5 9 1329.0 10 1314.0 11 1176.0 12 1254.5 13 1362.0 14 1416.0 15 1496.0 16 1577.0 17 1660.5 18 1682.5 19 1754.5 20 1911.5 21 2106.5 22 2442.5 23 2904.0 24 3456.5 25 4206.5 26 5270.5 27 6622.5 28 7741.5 29 9023.5 30 10631.5 31 12256.5 32 14236.0 33 15738.5 34 17174.5 35 18970.0 36 21214.0 37 23401.0 38 25450.5 39 26987.5 40 28134.0 41 30674.5 42 32556.5 43 34051.5 44 36568.0 45 38873.0 46 40003.0 47 41922.5 48 45530.0 49 45617.0 50 43141.0 51 41416.5 52 40714.5 53 41078.0 54 41119.5 55 38681.0 56 34542.5 57 31262.5 58 29025.5 59 26005.5 60 21343.5 61 17357.0 62 13948.5 63 10443.5 64 7550.0 65 5465.5 66 3941.0 67 3043.5 68 2303.5 69 1635.5 70 1236.0 71 849.0 72 591.5 73 498.0 74 364.5 75 241.5 76 175.5 77 116.5 78 72.5 79 44.5 80 32.5 81 21.0 82 12.5 83 9.0 84 5.5 85 2.0 86 1.5 87 1.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046822143272064354 2 0.0 3 0.0 4 0.0 5 0.011543920925065178 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006418420034336238 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011543920925065178 24-25 5.541082044031285E-4 26-27 0.0021702571339122533 28-29 0.002539662603514339 30-31 8.773379903049533E-4 32-33 0.0 34-35 0.0 36-37 1.3852705110078213E-4 38-39 2.7705410220156426E-4 40-41 0.0 42-43 0.014037407844879253 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 9.235136740052142E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.002838881033892E-4 84-85 4.617568370026071E-5 86-87 0.0 88-89 0.0 90-91 0.00909660968895136 92-93 0.0025858382872146 94-95 0.0 96-97 0.0 98-99 0.0017085002969096461 100-101 0.012513610282770654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1082821.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.86741860545868 #Duplication Level Percentage of deduplicated Percentage of total 1 79.70294831582027 47.7160977091245 2 11.450129026778079 13.709793350652728 3 3.5888614976028674 6.445676207820126 4 1.5767100812996544 3.7757424982645285 5 0.8951827508450975 2.6796140236614736 6 0.5709152977910364 2.0507535072669647 7 0.40989093586353936 1.7177378567948007 8 0.2964552899212108 1.4198410351612594 9 0.2121371638700816 1.1430093952066465 >10 1.2201778695917904 12.775358173893778 >50 0.04892009660076331 1.9801771501569563 >100 0.025673165424406903 2.8568607132588566 >500 0.0012298514407617276 0.4485787790296142 >1k 7.686571504760797E-4 1.2807595997078602 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4490 0.4146576396283411 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3828 0.35352103440919597 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2633 0.2431611503655729 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1805 0.16669421815794114 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1160 0.10712758618460486 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.617568370026071E-5 14-15 0.0 0.0 0.0 0.0 9.235136740052142E-5 16-17 0.0 0.0 0.0 0.0 9.235136740052142E-5 18-19 0.0 0.0 0.0 0.0 9.235136740052142E-5 20-21 0.0 0.0 0.0 0.0 9.235136740052142E-5 22-23 0.0 0.0 0.0 0.0 9.235136740052142E-5 24-25 0.0 0.0 0.0 0.0 9.235136740052142E-5 26-27 0.0 0.0 0.0 0.0 9.235136740052142E-5 28-29 0.0 0.0 0.0 3.23229785901825E-4 9.235136740052142E-5 30-31 0.0 0.0 0.0 6.926352555039106E-4 9.235136740052142E-5 32-33 0.0 0.0 0.0 0.0011543920925065178 9.235136740052142E-5 34-35 0.0 0.0 0.0 0.0018008516643101677 9.235136740052142E-5 36-37 0.0 0.0 0.0 0.0025858382872145994 9.235136740052142E-5 38-39 0.0 0.0 0.0 0.0036017033286203353 9.235136740052142E-5 40-41 0.0 0.0 0.0 0.005633433411431806 9.235136740052142E-5 42-43 0.0 0.0 0.0 0.008080744647545624 9.235136740052142E-5 44-45 0.0 0.0 0.0 0.015376502672186817 9.235136740052142E-5 46-47 0.0 0.0 0.0 0.028351869791960076 9.235136740052142E-5 48-49 0.0 0.0 0.0 0.04008049345182629 9.235136740052142E-5 50-51 0.0 0.0 0.0 0.05725784778832328 9.235136740052142E-5 52-53 0.0 0.0 0.0 0.07817543250454137 9.235136740052142E-5 54-55 0.0 0.0 0.0 0.0987236117511574 9.235136740052142E-5 56-57 0.0 0.0 0.0 0.12647519765501408 9.235136740052142E-5 58-59 0.0 0.0 0.0 0.17482113848918704 9.235136740052142E-5 60-61 0.0 0.0 0.0 0.23314102700261632 9.235136740052142E-5 62-63 0.0 0.0 0.0 0.3100235403635504 1.8470273480104284E-4 64-65 0.0 0.0 0.0 0.3809493905271508 1.8470273480104284E-4 66-67 0.0 0.0 0.0 0.4772718667258947 2.3087841850130355E-4 68-69 0.0 0.0 0.0 0.5946966303756577 2.7705410220156426E-4 70-71 0.0 0.0 0.0 0.7005312974166552 2.7705410220156426E-4 72-73 0.0 0.0 0.0 0.8522645940557119 2.7705410220156426E-4 74-75 0.0 0.0 0.0 0.9944395241688146 2.7705410220156426E-4 76-77 0.0 0.0 0.0 1.1440487393576593 3.694054696020857E-4 78-79 0.0 0.0 0.0 1.326765919759591 3.694054696020857E-4 80-81 0.0 0.0 0.0 1.5032955585456875 3.694054696020857E-4 82-83 0.0 0.0 0.0 1.7298334627791667 3.694054696020857E-4 84-85 0.0 0.0 0.0 2.0150144853119767 3.694054696020857E-4 86-87 0.0 0.0 0.0 2.329840296780354 4.155811533023464E-4 88-89 0.0 0.0 0.0 2.7421891522236823 4.617568370026071E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCG 65 0.0058439793 29.230713 8 GGTATCA 4740 0.0 27.476786 1 GTATCAA 6440 0.0 27.235428 1 TTACGCT 70 0.008383879 27.147821 4 TAAGGTG 455 0.0 26.103672 5 ATACCGT 135 1.3431356E-5 24.628443 6 GGACCGT 100 0.0016540469 23.74886 6 TCAACGC 7740 0.0 22.465345 4 ATCAACG 7775 0.0 22.364214 3 AGGTAAG 490 0.0 22.299994 2 CAACGCA 7815 0.0 22.249748 5 TATCAAC 7820 0.0 22.235521 2 GTACATG 4425 0.0 21.806023 1 AACGCAG 7970 0.0 21.752403 6 ATCACCG 155 3.9006496E-5 21.455534 4 ACATGGG 4365 0.0 21.441492 3 GAGTACT 4195 0.0 21.287205 12-13 TACATGG 4490 0.0 21.161997 2 GGTAAGG 520 0.0 21.013458 3 TACCGTC 115 0.003723183 20.652134 7 >>END_MODULE