FastQCFastQC Report
Thu 26 May 2016
SRR937177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129965
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.37319739991946654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.30248724518016046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22330.197616740341515No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17160.15186311080431697No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13860.12265866641887138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG702.0465034E-433.9477465
GGTATCA38900.032.1468121
GTATCAA57450.032.0296861
TATCAAC69750.026.3685652
TCAACGC69150.026.323624
ATCAACG69200.026.3034323
CAACGCA70000.026.071875
TAAGGTG4650.025.5520675
AACGCAG71800.025.4720176
GTACATG46600.024.0800761
TACATGG46500.023.9156742
ACATGGG46750.022.9745273
ACGCAGA80850.022.4445257
GAGTACT40900.022.29901712-13
CGCAGAG81850.022.1122728
GGACCGT1553.904017E-521.4532646
GTACTTT42350.021.198114-15
AGAGTAC65400.020.91904310-11
AGTACTT43100.020.88525412-13
AGGTAAG4100.020.864522
Produced by FastQC (version 0.11.5)