FastQCFastQC Report
Thu 26 May 2016
SRR937177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129965
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.4211634873646529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40950.3624006053284836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26940.2384144641648193No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18370.16257140707898032No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11640.10301204019593528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64250.029.515531
GGTATCA44900.028.5803931
GTACATG47500.025.014811
TACATGG47250.024.331682
TATCAAC78000.024.2408032
TCAACGC78000.024.2408034
ATCAACG78500.024.0864033
CAACGCA79000.023.933965
GTAAGGT4650.023.4981374
AACGCAG80950.023.353286
ACATGGG46800.022.5354213
TAAGGTG5300.022.4090125
GAGTACT45100.021.95893112-13
TACCGTG1302.9420128E-421.9224747
ACGCAGA89900.020.9226727
CGCAGAG90400.020.7544068
AGAGTAC68050.020.7305110-11
TTGCGCG704.8546333E-420.35658518-19
GTACTTT48150.020.2720314-15
TGTAGGG4200.019.2290652
Produced by FastQC (version 0.11.5)