##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937177_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1129965 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.231873553605645 33.0 31.0 34.0 31.0 34.0 2 32.4581513586704 34.0 31.0 34.0 31.0 34.0 3 32.535239587066854 34.0 31.0 34.0 31.0 34.0 4 36.12882876903267 37.0 35.0 37.0 35.0 37.0 5 36.02668578230299 37.0 35.0 37.0 35.0 37.0 6 36.10810600328329 37.0 35.0 37.0 35.0 37.0 7 36.06524449872341 37.0 35.0 37.0 35.0 37.0 8 36.04435270118986 37.0 35.0 37.0 35.0 37.0 9 37.75985804870062 39.0 37.0 39.0 35.0 39.0 10-11 37.68093879013952 39.0 37.0 39.0 35.0 39.0 12-13 37.60299080060002 39.0 37.0 39.0 35.0 39.0 14-15 38.83893748921427 40.0 38.0 41.0 35.0 41.0 16-17 38.812159668662304 40.0 38.0 41.0 35.0 41.0 18-19 38.80110401649608 40.0 38.0 41.0 35.0 41.0 20-21 38.684058798281356 40.0 38.0 41.0 34.0 41.0 22-23 38.555979609987915 40.0 38.0 41.0 34.0 41.0 24-25 38.40934719216967 40.0 38.0 41.0 34.0 41.0 26-27 38.35282154757006 40.0 38.0 41.0 34.0 41.0 28-29 38.265229896501225 40.0 38.0 41.0 34.0 41.0 30-31 38.371388936825475 40.0 38.0 41.0 34.0 41.0 32-33 38.31025606987827 40.0 38.0 41.0 34.0 41.0 34-35 38.19725168478669 40.0 38.0 41.0 34.0 41.0 36-37 38.04777979848933 40.0 37.5 41.0 33.5 41.0 38-39 37.917200532759864 40.0 37.0 41.0 33.0 41.0 40-41 37.75508444951835 40.0 37.0 41.0 33.0 41.0 42-43 37.55339545915139 40.0 37.0 41.0 33.0 41.0 44-45 37.27463018766068 40.0 37.0 41.0 32.5 41.0 46-47 36.93715778807308 40.0 36.0 41.0 31.0 41.0 48-49 36.636826804370045 39.5 36.0 41.0 31.0 41.0 50-51 36.40616877513905 39.0 36.0 41.0 30.0 41.0 52-53 36.166112667206505 39.0 35.0 41.0 30.0 41.0 54-55 35.921570579619726 39.0 35.0 40.0 29.0 41.0 56-57 35.642878319239976 38.5 35.0 40.0 28.5 41.0 58-59 35.36916320417004 38.0 35.0 40.0 28.0 41.0 60-61 35.01605049713929 38.0 34.0 40.0 28.0 41.0 62-63 34.63204701030563 37.0 34.0 40.0 27.0 41.0 64-65 34.25539198116756 37.0 34.0 39.5 26.0 41.0 66-67 33.80323240100357 36.0 33.0 39.0 26.0 40.5 68-69 33.38100118145252 35.5 33.0 39.0 25.5 40.0 70-71 32.961852800750464 35.0 32.5 38.0 25.0 40.0 72-73 32.50368197245048 35.0 32.0 37.0 24.0 39.5 74-75 32.06010849893581 35.0 32.0 37.0 23.0 39.0 76-77 30.778010380852507 33.5 30.0 35.5 21.0 37.5 78-79 31.410996800785867 34.0 31.0 36.0 22.0 38.0 80-81 31.283091954175575 34.0 31.0 35.5 22.0 37.0 82-83 30.96291168310523 34.0 31.0 35.0 20.0 37.0 84-85 30.619759904067827 34.0 31.0 35.0 19.5 36.0 86-87 30.263289128424333 34.0 31.0 35.0 18.0 36.0 88-89 29.883053014916392 34.0 30.0 35.0 14.5 35.5 90-91 29.440884894664876 34.0 30.0 35.0 7.0 35.0 92-93 29.074059816012003 34.0 29.0 35.0 2.0 35.0 94-95 28.652834379825926 34.0 29.0 35.0 2.0 35.0 96-97 28.16133597058316 33.5 29.0 35.0 2.0 35.0 98-99 27.626082223785694 33.0 28.0 35.0 2.0 35.0 100-101 25.701034102826192 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.146093476144884 1101 2 0.14188111747301235 1101 3 0.029590695677804035 1101 4 0.033051350809920166 1101 5 -0.07570722503211158 1101 6 -0.036138679225175 1101 7 -0.0887639488568297 1101 8 -0.06897067770194099 1101 9 0.015290319487888837 1101 10-11 -0.03004734233783779 1101 12-13 -0.13805085006126916 1101 14-15 -0.014962354659779464 1101 16-17 -0.005348667792901551 1101 18-19 -0.055964200460060454 1101 20-21 0.011421595272423701 1101 22-23 -0.08296810695316736 1101 24-25 0.10120281688923427 1101 26-27 0.029918420292673886 1101 28-29 -0.07097513361735253 1101 30-31 0.11287102005908167 1101 32-33 0.04814757130665015 1101 34-35 0.015768521118445733 1101 36-37 0.10877108217108145 1101 38-39 -0.03693222520463024 1101 40-41 -0.06909536063290034 1101 42-43 -0.024032303665364907 1101 44-45 -0.07622257588126757 1101 46-47 0.018235443655974848 1101 48-49 0.13248382296838912 1101 50-51 0.09075238036496103 1101 52-53 0.01805562515654202 1101 54-55 -0.04815292162201246 1101 56-57 -0.04966485858582104 1101 58-59 -0.06200665141722084 1101 60-61 -0.1535689592242946 1101 62-63 -0.1524947739886926 1101 64-65 -0.07098536750675066 1101 66-67 0.004144958842665858 1101 68-69 0.07190456383442978 1101 70-71 -0.17544105160117596 1101 72-73 -0.22029850603045986 1101 74-75 -0.21147556904484333 1101 76-77 -0.3118678691577408 1101 78-79 -0.37391313721549224 1101 80-81 -0.2314416332852467 1101 82-83 -0.06432660082315778 1101 84-85 -0.2123673471637737 1101 86-87 -0.1787433700082488 1101 88-89 -0.13730072564143114 1101 90-91 -0.30012900509273166 1101 92-93 -0.48985270587608554 1101 94-95 -0.760959397318576 1101 96-97 -0.537734173878242 1101 98-99 -0.28438908511950345 1101 100-101 0.37026371871479924 1102 1 0.1703968616018372 1102 2 0.14338944343230509 1102 3 0.13170049561963992 1102 4 0.09620766597733876 1102 5 -0.005965326676260929 1102 6 0.034237489534064025 1102 7 0.08380401089639378 1102 8 0.013702601878264886 1102 9 0.07537738470325905 1102 10-11 -0.048767601243923764 1102 12-13 0.10188049403506483 1102 14-15 0.04394048130242112 1102 16-17 0.04371060090878842 1102 18-19 0.08368048435396247 1102 20-21 0.05610594519166767 1102 22-23 0.12096297724700378 1102 24-25 0.08255526702939875 1102 26-27 -0.01628508459275224 1102 28-29 -0.016775954146673655 1102 30-31 0.05990866787502114 1102 32-33 0.04416172327164958 1102 34-35 0.017214967038384543 1102 36-37 -0.00763687436438687 1102 38-39 -0.015147357349455604 1102 40-41 0.1752101287453911 1102 42-43 0.19679589878136738 1102 44-45 0.14956917922228996 1102 46-47 0.1562557774369111 1102 48-49 0.09781088991640985 1102 50-51 0.07256815729979849 1102 52-53 0.19807780915065365 1102 54-55 0.13343792136976163 1102 56-57 -0.044732245948686966 1102 58-59 -0.015349875243515498 1102 60-61 -0.0023441985453445113 1102 62-63 -0.06360719145619242 1102 64-65 0.125980344763434 1102 66-67 0.16557992601530458 1102 68-69 0.15040403476010056 1102 70-71 0.055929962550791856 1102 72-73 0.2501437196708025 1102 74-75 0.05730038189666686 1102 76-77 0.05458959140920072 1102 78-79 0.21297649354631076 1102 80-81 0.2920735551704112 1102 82-83 0.32105068177679286 1102 84-85 0.20555187463284952 1102 86-87 0.2980709527456611 1102 88-89 0.36635638468449017 1102 90-91 0.3789827688223575 1102 92-93 0.4262480162639726 1102 94-95 0.5176717233908157 1102 96-97 0.2880139557975667 1102 98-99 0.28039235458149037 1102 100-101 0.7113959996749308 1103 1 -0.03248510829636331 1103 2 0.02878160420426923 1103 3 0.07427657060728876 1103 4 0.09109459252022845 1103 5 -4.6501535235421443E-4 1103 6 -0.10843090909549602 1103 7 0.0035192492048778945 1103 8 -0.006256634626687685 1103 9 0.02966337942148556 1103 10-11 0.10217771923566943 1103 12-13 0.052289892643152314 1103 14-15 -0.09559282422135595 1103 16-17 -0.025885628851320064 1103 18-19 -0.0042412981977904 1103 20-21 0.044752591021612886 1103 22-23 0.030699747514496778 1103 24-25 -0.05059244773042337 1103 26-27 0.04029228476622393 1103 28-29 -0.13021747892836544 1103 30-31 -0.10609177073895637 1103 32-33 -0.09469487449479885 1103 34-35 0.0797860884485786 1103 36-37 0.0330530071485029 1103 38-39 -0.008529807269248124 1103 40-41 -0.03761465263774966 1103 42-43 0.14899387624949867 1103 44-45 0.011746238177529733 1103 46-47 0.1290854817251983 1103 48-49 0.27598670050878127 1103 50-51 0.17933538823466222 1103 52-53 0.2477945504021406 1103 54-55 0.20255336272891356 1103 56-57 -0.0737440302487542 1103 58-59 0.1861353545073996 1103 60-61 0.062271615482330844 1103 62-63 0.02859414462947285 1103 64-65 -0.04045985804184937 1103 66-67 -0.14663808046882565 1103 68-69 -0.010946482221626752 1103 70-71 -0.12850184831150102 1103 72-73 -0.20438842030533522 1103 74-75 -0.14119314778032788 1103 76-77 -0.2841166353756961 1103 78-79 -0.29063708753746553 1103 80-81 -0.2425903683696653 1103 82-83 -0.22052294556081975 1103 84-85 -0.22244143573714936 1103 86-87 -0.23222408416846108 1103 88-89 -0.20230894057998228 1103 90-91 -0.1961275747994904 1103 92-93 -0.15938599564301725 1103 94-95 -0.14900427276901596 1103 96-97 -0.12700458561158 1103 98-99 -0.14957855273599918 1103 100-101 0.2008163544308239 1104 1 0.1395978854685822 1104 2 0.11150280458016226 1104 3 -0.007350862099677613 1104 4 -0.0017555593594522634 1104 5 0.06372062490141417 1104 6 0.05112620273396118 1104 7 0.07879035151212577 1104 8 0.10602007884517661 1104 9 0.13994752505441 1104 10-11 0.03165329565707964 1104 12-13 0.11580425979814635 1104 14-15 0.10904584251289862 1104 16-17 0.09858766645035644 1104 18-19 0.07873316495919624 1104 20-21 -0.009090413349561288 1104 22-23 0.021093308907815356 1104 24-25 0.0025543277021142785 1104 26-27 -0.033124091723813365 1104 28-29 0.041878772626503746 1104 30-31 0.18397755970365637 1104 32-33 0.16388720487257302 1104 34-35 -0.0057063799569334606 1104 36-37 -0.004017333225760922 1104 38-39 -0.08182393913746466 1104 40-41 0.05191500776786029 1104 42-43 0.027424621844204466 1104 44-45 0.04940440556102743 1104 46-47 0.0572506815863818 1104 48-49 -0.042282534009821404 1104 50-51 -0.12782440322810373 1104 52-53 -0.07904370066140842 1104 54-55 0.06799342387072471 1104 56-57 -0.004363286018822521 1104 58-59 0.06192838480924934 1104 60-61 0.006351232410189311 1104 62-63 -0.12169421230142774 1104 64-65 -0.1261640611511865 1104 66-67 0.013535323624715545 1104 68-69 0.13071514778464888 1104 70-71 -0.0730302804218681 1104 72-73 -0.049888765397810175 1104 74-75 -0.06763249534108695 1104 76-77 0.05371895243181868 1104 78-79 0.09014399125669925 1104 80-81 0.05935306765955062 1104 82-83 0.23773685253083698 1104 84-85 0.24622650731674156 1104 86-87 0.3052180592262381 1104 88-89 0.40118429209812945 1104 90-91 0.29767577318240157 1104 92-93 0.24975037632377806 1104 94-95 0.41534864959882967 1104 96-97 0.6526827255136567 1104 98-99 0.7135899420759166 1104 100-101 0.9722984583640972 1105 1 0.02831087299367141 1105 2 0.10205879827101683 1105 3 0.09193129304801317 1105 4 0.143992038862514 1105 5 -0.050413569965236604 1105 6 0.015397962125987874 1105 7 -0.012342785855238958 1105 8 0.020803112254981215 1105 9 0.03263509674927434 1105 10-11 -0.003173830095711594 1105 12-13 0.08879680353201991 1105 14-15 0.09543636445152259 1105 16-17 0.17199738468952575 1105 18-19 0.060422744110333326 1105 20-21 0.06680958127332559 1105 22-23 0.07945785938100158 1105 24-25 -0.03553984350522654 1105 26-27 0.03418775406052532 1105 28-29 0.15555725699702805 1105 30-31 0.21545989588089043 1105 32-33 0.15018385134518297 1105 34-35 0.19643191944082616 1105 36-37 0.05384624936672111 1105 38-39 0.10662489761332239 1105 40-41 0.2170544990927823 1105 42-43 0.02753798935209062 1105 44-45 0.29155471384896003 1105 46-47 0.4181111096047374 1105 48-49 0.44169747208423615 1105 50-51 0.2533359781885878 1105 52-53 0.38769615451496264 1105 54-55 0.2824587594026937 1105 56-57 0.17152931507094138 1105 58-59 0.18627977031254517 1105 60-61 0.19570611303823426 1105 62-63 0.19017128812874518 1105 64-65 0.13678179130077694 1105 66-67 0.170719821402173 1105 68-69 0.323851932628358 1105 70-71 0.337508640928867 1105 72-73 0.11855429095435 1105 74-75 0.24872513657746964 1105 76-77 0.3485575941725685 1105 78-79 0.24336734765187984 1105 80-81 0.29557622689385 1105 82-83 0.3935282294307534 1105 84-85 0.25048652667435434 1105 86-87 0.5651807330862901 1105 88-89 0.632632573213705 1105 90-91 0.5810212797066363 1105 92-93 0.4311473508004049 1105 94-95 0.42937619835133845 1105 96-97 0.5046332739793868 1105 98-99 0.532210219176573 1105 100-101 1.0025944685447712 1106 1 -0.14573441456541758 1106 2 -0.05843008643132208 1106 3 -0.13445191069247642 1106 4 -0.03305739766553728 1106 5 -0.14178026774560237 1106 6 -0.2319420306807487 1106 7 -0.17182118895176757 1106 8 -0.09709836150675244 1106 9 -0.26083220389469375 1106 10-11 -0.3105630838807443 1106 12-13 -0.41033829272063826 1106 14-15 -0.3463517598205854 1106 16-17 -0.18645651422805543 1106 18-19 -0.2522437070440091 1106 20-21 -0.24565589750655903 1106 22-23 -0.2044473308978212 1106 24-25 -0.14252565394573224 1106 26-27 -0.2732584998786365 1106 28-29 -0.2782315780375768 1106 30-31 -0.09573284706286955 1106 32-33 -0.21049251837118987 1106 34-35 -0.2794342688170417 1106 36-37 -0.29265634962622045 1106 38-39 -0.17987366587575337 1106 40-41 -0.28323103689064055 1106 42-43 -0.6104200456893594 1106 44-45 -0.3597509910904151 1106 46-47 -0.30612321508526463 1106 48-49 -0.47826441006620257 1106 50-51 -0.5200057187354403 1106 52-53 -0.4384935163078083 1106 54-55 -0.3875622637527485 1106 56-57 -0.3450708424956801 1106 58-59 -0.4043079253612234 1106 60-61 -0.5048297954532401 1106 62-63 -0.37286392458620554 1106 64-65 -0.41828356887597806 1106 66-67 -0.4122159380860779 1106 68-69 -0.481401554817694 1106 70-71 -0.24794582959589917 1106 72-73 -0.21024240100290115 1106 74-75 -0.4536489599834681 1106 76-77 -0.4968293539160804 1106 78-79 -0.39461825084438473 1106 80-81 -0.5068368866740052 1106 82-83 -0.6255272164656631 1106 84-85 -0.7769832659200127 1106 86-87 -0.6596720298958338 1106 88-89 -0.41508779526954953 1106 90-91 -0.46325124846696397 1106 92-93 -0.6060413694845863 1106 94-95 -1.218851498264403 1106 96-97 -1.0013605732152442 1106 98-99 -0.4370800780591573 1106 100-101 -0.047384229989173576 1107 1 -0.06869606246410598 1107 2 -0.024208995323292015 1107 3 -0.07544475839164733 1107 4 0.009315760958926944 1107 5 -0.11808837180929999 1107 6 -0.037725015874656265 1107 7 -0.08553714043304694 1107 8 -0.11003793837588205 1107 9 -0.1906002033154266 1107 10-11 -0.14906563291034303 1107 12-13 -0.28481206091314704 1107 14-15 -0.24706403994834147 1107 16-17 -0.265769013725226 1107 18-19 -0.21177729228565312 1107 20-21 -0.1337477322983318 1107 22-23 -0.25228702496062994 1107 24-25 -0.17780872897918698 1107 26-27 -0.2500611237298287 1107 28-29 -0.2705700076422559 1107 30-31 -0.24520842545525312 1107 32-33 -0.3594936903080068 1107 34-35 -0.39384024167208054 1107 36-37 -0.4221207718238347 1107 38-39 -0.36311765131906526 1107 40-41 -0.07668159966166854 1107 42-43 -0.13818153911766018 1107 44-45 -0.13963765916948745 1107 46-47 -0.2551868724399071 1107 48-49 -0.20482393077890748 1107 50-51 -0.11718529324460292 1107 52-53 -0.08625543882052256 1107 54-55 -0.14563186660830496 1107 56-57 0.0045323885099648464 1107 58-59 0.009484993136879893 1107 60-61 0.11710821793955972 1107 62-63 -0.1344404879954908 1107 64-65 -0.10508117780307202 1107 66-67 -0.12760773238300516 1107 68-69 -0.12806770300735337 1107 70-71 -0.2989325896599411 1107 72-73 -0.376590383099348 1107 74-75 -0.33354850059496854 1107 76-77 -0.18568066812119 1107 78-79 -0.2555546739430703 1107 80-81 -0.3501151452349127 1107 82-83 -0.25266358753786733 1107 84-85 -0.16863703770610172 1107 86-87 -0.2906484382983763 1107 88-89 -0.06267057503929152 1107 90-91 -0.18615282454765136 1107 92-93 -0.09586858255449826 1107 94-95 -0.10740245337949261 1107 96-97 -0.19276896235189867 1107 98-99 0.09171336338556912 1107 100-101 0.6534361257754391 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0.13492983299452987 2316 60-61 -0.1215038817045695 2316 62-63 -0.25810596842253375 2316 64-65 -0.05365769025858924 2316 66-67 -0.1822340669025806 2316 68-69 -0.12112820122799661 2316 70-71 0.0387898302688825 2316 72-73 -0.016270098401840016 2316 74-75 0.15150488018795016 2316 76-77 0.3600488692453574 2316 78-79 0.05405386698219772 2316 80-81 -4.710171210327019E-4 2316 82-83 -0.16547497543822232 2316 84-85 -0.14265839001927105 2316 86-87 -0.06306836712399999 2316 88-89 0.0026319160639118877 2316 90-91 0.05067991566587082 2316 92-93 0.19363319680493518 2316 94-95 0.21394985978776404 2316 96-97 0.21239117999685675 2316 98-99 -0.3196745369703038 2316 100-101 -0.6068841589220675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 13.0 5 27.0 6 42.0 7 83.0 8 251.0 9 475.0 10 860.0 11 1359.0 12 1995.0 13 2960.0 14 3740.0 15 5349.0 16 7024.0 17 10103.0 18 9173.0 19 5830.0 20 4927.0 21 5087.0 22 5330.0 23 5693.0 24 6594.0 25 7932.0 26 9491.0 27 11837.0 28 14782.0 29 19068.0 30 23789.0 31 30499.0 32 39569.0 33 53484.0 34 74792.0 35 109480.0 36 174278.0 37 266463.0 38 200381.0 39 17198.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33168936202993 19.127768446199195 15.249442392677745 22.291099799093132 2 16.086869947299252 20.286823043191603 39.10085710619355 24.52544990331559 3 19.976193952910045 23.94330797856571 29.491798418535087 26.588699649989163 4 13.705911245038562 16.748837353369353 35.8102242104844 33.73502719110769 5 14.24393620345218 37.28047697824088 33.494515299009805 14.981071519297137 6 33.109875084626516 35.57632316045187 16.49245773099167 14.821344023929944 7 29.330996977782497 32.39976459447859 19.83397715858456 18.435261269154353 8 27.28978331187249 34.556911054767184 18.849256392897125 19.3040492404632 9 26.528520794891875 16.765298040204783 19.55724292345338 37.148938241449954 10-11 25.636634763023636 26.512945091219642 26.273380148942664 21.577039996814058 12-13 26.456739115139484 23.65244911297563 27.229309439217225 22.661502332667656 14-15 23.253950343594713 25.14493811755231 25.49331173974415 26.10779979910882 16-17 22.569238870230492 28.45136796272451 26.237007340935335 22.74238582610966 18-19 22.644904930683694 26.72830574398322 28.74549211701247 21.88129720832061 20-21 23.29417282836194 25.70269875615616 29.02603177974539 21.977096635736505 22-23 22.993686012187247 25.455340339828748 29.165897828358116 22.385075819625886 24-25 23.083877008471067 25.733828688347593 29.017959864208066 22.164334438973277 26-27 22.58936809011038 26.07214051980291 29.058518205491847 22.27997318459486 28-29 22.480331667884297 26.466479427183476 28.932438466075862 22.12075043885637 30-31 22.664197563641352 25.8539866279044 28.979791409468437 22.50202439898581 32-33 22.605744425712302 25.95129052669773 28.908550264831213 22.53441478275876 34-35 22.99022536096251 26.00018584646427 28.741598191094415 22.267990601478807 36-37 23.007969273384575 25.782789732425343 28.80637895864031 22.402862035549774 38-39 22.723978176315196 26.018151004677136 28.878195342333612 22.37967547667406 40-41 22.905709468877355 26.02624860062037 28.69310111375131 22.37494081675096 42-43 22.72737934492585 26.064841798915804 28.467963467239876 22.739815388918466 44-45 22.89924909178603 26.058329240286206 28.479908669737558 22.562512998190208 46-47 23.351563986495158 26.512281353847243 27.82851681246764 22.307637847189955 48-49 23.26377365670618 26.602593885651327 27.52279937874182 22.610833078900676 50-51 23.048855495524197 26.857601784126057 27.67634395755621 22.41719876279354 52-53 23.380148942666366 26.898045514684082 27.259959379272807 22.46184616337674 54-55 23.156690693959547 26.821317474435048 27.29562420074958 22.726367630855822 56-57 23.275897926041957 27.087387662449725 27.157035837393195 22.479678574115127 58-59 23.378821467921572 26.615072148252377 27.264118800139826 22.74198758368622 60-61 22.974915152239227 26.65095821551995 27.839888846114704 22.53423778612612 62-63 22.780307354652578 26.81755629599147 27.918785095113563 22.483351254242386 64-65 22.860221334289115 26.890877151062202 27.639838402074403 22.609063112574283 66-67 22.899912829158424 26.825875137725504 27.41695539242366 22.857256640692412 68-69 22.884292876328026 26.696756094215306 27.636873708477694 22.78207732097897 70-71 22.97478240476475 26.773749629413302 27.455673405813453 22.795794560008495 72-73 22.96128641152602 26.697021589164265 27.48908152022408 22.852610479085637 74-75 22.861593058192067 27.3074829751364 26.93809100281867 22.892832963852864 76-77 23.08806024965375 26.91065652475962 27.2277459921325 22.77353723345413 78-79 23.002438128614603 26.877956396879547 27.16128375657653 22.958321717929316 80-81 23.09934378498449 27.10681304288186 27.052475076661665 22.741368095471984 82-83 23.005395761446636 27.011330479547524 27.068544843583098 22.914728915422742 84-85 22.921738807218393 27.106182856364057 27.016134385064078 22.955943951353472 86-87 23.060448774962055 27.08632568265389 26.956896895036568 22.896328647347485 88-89 22.884160128853548 27.00499572995624 26.81361812091525 23.297226020274962 90-91 22.959243958728397 26.910758895677013 26.8911984741101 23.23879867148449 92-93 22.927283854087154 26.84401103236121 27.086676959171147 23.142028154380487 94-95 22.755926068506547 26.830831043439396 27.052298080029026 23.360944808025028 96-97 22.81013128725226 26.801051359997874 27.018889965618403 23.369927387131458 98-99 22.631979985185353 27.04451384691357 26.762115219701883 23.56139094819919 100-101 22.50995866066027 26.911861482896683 26.98691931415907 23.59126054228398 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 240.0 1 175.5 2 166.5 3 220.0 4 174.5 5 162.0 6 321.0 7 686.5 8 1084.5 9 1328.5 10 1334.0 11 1267.0 12 1298.5 13 1389.0 14 1532.5 15 1605.0 16 1641.0 17 1707.5 18 1785.5 19 1874.5 20 1995.5 21 2211.5 22 2527.0 23 3036.0 24 3591.0 25 4349.5 26 5504.0 27 6905.5 28 8118.5 29 9513.0 30 11177.5 31 12848.0 32 15018.0 33 16483.5 34 17709.0 35 19513.0 36 21992.0 37 24476.5 38 26621.0 39 28352.0 40 29629.0 41 32143.0 42 33755.5 43 35361.0 44 38352.0 45 40635.5 46 41646.0 47 43638.0 48 47210.5 49 47035.0 50 44671.0 51 43125.0 52 42394.5 53 42874.5 54 43141.5 55 40551.0 56 35840.0 57 32432.0 58 30484.0 59 27311.5 60 22366.5 61 18306.0 62 14755.0 63 10963.0 64 7815.0 65 5705.0 66 4165.5 67 3234.0 68 2455.0 69 1736.0 70 1291.5 71 881.5 72 646.5 73 503.5 74 348.0 75 252.0 76 186.0 77 120.0 78 72.5 79 42.0 80 28.5 81 17.5 82 9.5 83 7.5 84 5.5 85 2.5 86 0.5 87 0.0 88 0.0 89 0.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05186001336324576 2 0.0 3 0.0 4 0.0 5 0.016460686835432954 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003141690229343387 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011947272703136823 24-25 8.849831631953202E-5 26-27 0.0015487205355918104 28-29 0.003495683494621515 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.016327939360953656 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.5399326527812806E-4 84-85 0.0015929696937515764 86-87 0.0 88-89 0.0 90-91 0.011726026912337992 92-93 0.0014602222192722782 94-95 0.0 96-97 0.0 98-99 1.7699663263906403E-4 100-101 0.015000464616160678 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1129965.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.28525739038354 #Duplication Level Percentage of deduplicated Percentage of total 1 79.43222910746809 47.091601477281614 2 11.533358283963384 13.675162288805629 3 3.6231057156462785 6.4439026471397804 4 1.610437899369065 3.819009015012944 5 0.9520419780667797 2.822102685806946 6 0.5997840241744773 2.1335010151094345 7 0.4019921927218002 1.668254743010561 8 0.2971812962936162 1.4094775713899896 9 0.2346355046944856 1.2519383659861612 >10 1.232539663865697 12.80870118976776 >50 0.0540533061144222 2.1450603935866943 >100 0.02655262171439666 2.9443322916703223 >500 0.0013425466547825266 0.5002876312184571 >1k 7.458592526569591E-4 1.2866686842137762 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4759 0.4211634873646529 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4095 0.3624006053284836 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2694 0.2384144641648193 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1837 0.16257140707898032 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1164 0.10301204019593528 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 8.849831631953202E-5 0.0 26-27 0.0 0.0 0.0 8.849831631953202E-5 0.0 28-29 0.0 0.0 0.0 3.5399326527812806E-4 0.0 30-31 0.0 0.0 0.0 9.292323213550863E-4 0.0 32-33 0.0 0.0 0.0 0.0012832255866332143 0.0 34-35 0.0 0.0 0.0 0.0022124579079883006 8.849831631953202E-5 36-37 0.0 0.0 0.0 0.0030089427548640885 8.849831631953202E-5 38-39 0.0 0.0 0.0 0.004203670025177771 1.3274747447929804E-4 40-41 0.0 0.0 0.0 0.006283380458686773 1.7699663263906403E-4 42-43 0.0 0.0 0.0 0.00920382489723133 1.7699663263906403E-4 44-45 0.0 0.0 0.0 0.017611164947586872 1.7699663263906403E-4 46-47 0.0 0.0 0.0 0.03296562282902568 1.7699663263906403E-4 48-49 0.0 0.0 0.0 0.04570938037903829 1.7699663263906403E-4 50-51 0.0 0.0 0.0 0.06451527259693884 1.7699663263906403E-4 52-53 0.0 0.0 0.0 0.0880115755797746 3.5399326527812806E-4 54-55 0.0 0.0 0.0 0.11323359573084121 3.5399326527812806E-4 56-57 0.0 0.0 0.0 0.14540273371299112 3.9824242343789406E-4 58-59 0.0 0.0 0.0 0.19708575044359783 4.424915815976601E-4 60-61 0.0 0.0 0.0 0.26009655166310464 4.424915815976601E-4 62-63 0.0 0.0 0.0 0.3405415211975592 4.8674073975742615E-4 64-65 0.0 0.0 0.0 0.409039218028877 5.309898979171921E-4 66-67 0.0 0.0 0.0 0.5061218710314037 5.309898979171921E-4 68-69 0.0 0.0 0.0 0.6354621603323997 5.309898979171921E-4 70-71 0.0 0.0 0.0 0.7447575809870217 5.309898979171921E-4 72-73 0.0 0.0 0.0 0.8987004022248477 7.079865305562561E-4 74-75 0.0 0.0 0.0 1.0421561729788091 8.849831631953202E-4 76-77 0.0 0.0 0.0 1.1910988393445814 8.849831631953202E-4 78-79 0.0 0.0 0.0 1.3805737345846996 8.849831631953202E-4 80-81 0.0 0.0 0.0 1.5649599766364446 8.849831631953202E-4 82-83 0.0 0.0 0.0 1.7894802051390972 8.849831631953202E-4 84-85 0.0 0.0 0.0 2.0798874301416417 8.849831631953202E-4 86-87 0.0 0.0 0.0 2.404720500192484 8.849831631953202E-4 88-89 0.0 0.0 0.0 2.824600761970504 9.292323213550862E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6425 0.0 29.51553 1 GGTATCA 4490 0.0 28.580393 1 GTACATG 4750 0.0 25.01481 1 TACATGG 4725 0.0 24.33168 2 TATCAAC 7800 0.0 24.240803 2 TCAACGC 7800 0.0 24.240803 4 ATCAACG 7850 0.0 24.086403 3 CAACGCA 7900 0.0 23.93396 5 GTAAGGT 465 0.0 23.498137 4 AACGCAG 8095 0.0 23.35328 6 ACATGGG 4680 0.0 22.535421 3 TAAGGTG 530 0.0 22.409012 5 GAGTACT 4510 0.0 21.958931 12-13 TACCGTG 130 2.9420128E-4 21.922474 7 ACGCAGA 8990 0.0 20.922672 7 CGCAGAG 9040 0.0 20.754406 8 AGAGTAC 6805 0.0 20.73051 10-11 TTGCGCG 70 4.8546333E-4 20.356585 18-19 GTACTTT 4815 0.0 20.27203 14-15 TGTAGGG 420 0.0 19.229065 2 >>END_MODULE