FastQCFastQC Report
Thu 26 May 2016
SRR937176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333537
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48280.3620446976724305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38150.28608130108125984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23740.17802280701622827No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19500.1462276637243661No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15670.1175070507979906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64050.031.8250241
GGTATCA43100.031.1989351
TCAACGC78000.025.8866564
ATCAACG77900.025.8569553
CAACGCA78150.025.6545895
TATCAAC79600.025.3653682
AACGCAG80350.025.1286016
GTATAGG2701.8189894E-1224.6373981
GTACATG55300.022.1679331
TACATGG54900.021.8068772
ACGCAGA91850.021.56787
CGCAGAG92300.021.5647778
GAGTACT45450.021.37350312-13
AGAGTAC74150.020.69224710-11
GTACTTT47000.020.21154414-15
AGTACTT47700.020.0665612-13
ACATGGG54850.019.8338853
GCAGAGT97700.019.6914089
TAAGGCG3254.8748916E-1019.003855
GGACCGA2654.0002305E-717.9274866
Produced by FastQC (version 0.11.5)