FastQCFastQC Report
Thu 26 May 2016
SRR937176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333537
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52260.39189013878130113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46910.3517712669389751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31610.23703879232447245No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19110.14330311044987878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75700.029.4478341
TACCGTG1503.3358447E-828.5014517
GGTATCA52800.026.8261741
TATTCCG1858.976713E-925.6740955
ATCAACG90600.024.377643
TCAACGC91350.024.2294884
TATCAAC91700.024.0852132
CAACGCA92050.024.0452335
AACGCAG93200.023.8504626
GTACCGT1252.2571077E-422.7985956
TACATGG55750.021.8955122
GTACATG56800.021.8408011
GAGTACT50700.021.40888212-13
ACGCAGA104600.021.1172337
TGTACCG1805.172533E-621.109815
AGAGTAC81100.021.0275810-11
ACATGGG54750.020.9941433
CGCAGAG105550.020.8821628
GTAAGGT4850.020.5657424
AGTACTT53400.020.54879812-13
Produced by FastQC (version 0.11.5)