##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937176_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1333537 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26365447677867 33.0 31.0 34.0 31.0 34.0 2 32.4756695914699 34.0 31.0 34.0 31.0 34.0 3 32.52977607670428 34.0 31.0 34.0 31.0 34.0 4 36.152535700171796 37.0 35.0 37.0 35.0 37.0 5 36.072998349502114 37.0 35.0 37.0 35.0 37.0 6 36.11451650760346 37.0 35.0 37.0 35.0 37.0 7 36.06289514276694 37.0 35.0 37.0 35.0 37.0 8 36.034849426750064 37.0 35.0 37.0 35.0 37.0 9 37.75466372511599 39.0 37.0 39.0 35.0 39.0 10-11 37.67359848283175 39.0 37.0 39.0 35.0 39.0 12-13 37.58310905509184 39.0 37.0 39.0 35.0 39.0 14-15 38.80492029842442 40.0 38.0 41.0 35.0 41.0 16-17 38.766705760695054 40.0 38.0 41.0 35.0 41.0 18-19 38.74747119877439 40.0 38.0 41.0 35.0 41.0 20-21 38.63246014171335 40.0 38.0 41.0 34.0 41.0 22-23 38.484702336718065 40.0 38.0 41.0 34.0 41.0 24-25 38.41675109127081 40.0 38.0 41.0 34.0 41.0 26-27 38.3663242939641 40.0 38.0 41.0 34.0 41.0 28-29 38.245771583390635 40.0 38.0 41.0 34.0 41.0 30-31 38.3390445859395 40.0 38.0 41.0 34.0 41.0 32-33 38.29614326411641 40.0 38.0 41.0 34.0 41.0 34-35 38.17977004012637 40.0 38.0 41.0 34.0 41.0 36-37 38.01802462173903 40.0 37.5 41.0 33.0 41.0 38-39 37.889909691294655 40.0 37.0 41.0 33.0 41.0 40-41 37.71879670380349 40.0 37.0 41.0 33.0 41.0 42-43 37.5152399221019 40.0 37.0 41.0 33.0 41.0 44-45 37.23790341025408 40.0 37.0 41.0 32.0 41.0 46-47 36.90861445914137 40.0 36.0 41.0 31.0 41.0 48-49 36.600185446673024 39.0 36.0 41.0 31.0 41.0 50-51 36.35464107857525 39.0 35.5 41.0 30.0 41.0 52-53 36.10119029318271 39.0 35.0 40.5 29.5 41.0 54-55 35.858249902327415 39.0 35.0 40.0 29.0 41.0 56-57 35.561957785948195 38.0 35.0 40.0 28.0 41.0 58-59 35.26624158159841 38.0 34.5 40.0 28.0 41.0 60-61 34.91757259078676 38.0 34.0 40.0 27.5 41.0 62-63 34.52906930966295 37.0 34.0 40.0 26.5 41.0 64-65 34.101305400600054 36.5 33.5 39.0 26.0 41.0 66-67 33.660727448882184 36.0 33.0 39.0 26.0 40.0 68-69 33.20413719304376 35.5 32.5 39.0 25.0 40.0 70-71 32.78209715965886 35.0 32.0 38.0 24.0 40.0 72-73 32.30410967224756 35.0 32.0 37.0 23.0 39.0 74-75 31.826431512586453 35.0 31.0 37.0 22.0 39.0 76-77 30.610183669444492 33.5 29.5 35.5 20.5 37.5 78-79 31.211949874656646 34.0 31.0 36.0 21.0 37.5 80-81 31.03657491318201 34.0 31.0 35.0 20.0 37.0 82-83 30.703589776661616 34.0 31.0 35.0 19.5 37.0 84-85 30.363271510276803 34.0 30.5 35.0 18.0 36.0 86-87 29.962578841082024 34.0 30.0 35.0 15.5 36.0 88-89 29.502101928930358 34.0 29.5 35.0 8.5 35.5 90-91 29.005943592116303 34.0 29.0 35.0 4.5 35.0 92-93 28.638910281454507 34.0 29.0 35.0 2.0 35.0 94-95 28.19202391834647 33.0 29.0 35.0 2.0 35.0 96-97 27.699987326935812 33.0 27.5 35.0 2.0 35.0 98-99 26.93093404982389 33.0 26.5 35.0 2.0 35.0 100-101 24.42915082221191 30.5 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1705131455733735 1101 2 -0.13180476761913695 1101 3 -0.18321237079622676 1101 4 -0.06593842118134319 1101 5 -0.15946802333105836 1101 6 -0.19180546965693424 1101 7 -0.16251825273324982 1101 8 -0.18651336607185698 1101 9 -0.15842193572244412 1101 10-11 -0.13757025353837804 1101 12-13 -0.24377575465246792 1101 14-15 -0.3704834817340412 1101 16-17 -0.38312808728066017 1101 18-19 -0.18472962558493577 1101 20-21 -0.21623258529805867 1101 22-23 -0.2861187287135962 1101 24-25 -0.28279177775755926 1101 26-27 -0.2982703496745174 1101 28-29 -0.4025065391684848 1101 30-31 -0.3471091092567704 1101 32-33 -0.3599595959713966 1101 34-35 -0.40452744512329986 1101 36-37 -0.49935470888787137 1101 38-39 -0.5371020632780201 1101 40-41 -0.3850147956970673 1101 42-43 -0.4287824278589696 1101 44-45 -0.4204807547407299 1101 46-47 -0.5840905120349618 1101 48-49 -0.6187784116905846 1101 50-51 -0.4889993085411177 1101 52-53 -0.5515123800591795 1101 54-55 -0.7735772673171368 1101 56-57 -0.6822293830433921 1101 58-59 -0.7436656947092288 1101 60-61 -0.6337887724126787 1101 62-63 -0.6101771187435929 1101 64-65 -0.7825811534227114 1101 66-67 -0.8499380318006544 1101 68-69 -0.8523827164809532 1101 70-71 -0.741087135847323 1101 72-73 -0.7068162124708763 1101 74-75 -0.7648242703975292 1101 76-77 -0.7056402945543141 1101 78-79 -0.9416486890091846 1101 80-81 -0.9308034490008978 1101 82-83 -0.8486982873150559 1101 84-85 -0.9107426412995245 1101 86-87 -1.066102844710855 1101 88-89 -1.0208629931927575 1101 90-91 -1.0153874107447969 1101 92-93 -1.106722571154247 1101 94-95 -1.125063908173967 1101 96-97 -1.2622331184064812 1101 98-99 -1.0778192481569597 1101 100-101 -0.810007051516596 1102 1 -0.09878911964519688 1102 2 -0.0705445114442469 1102 3 -0.13857453204236236 1102 4 -0.025817536008133857 1102 5 -0.05091709197858307 1102 6 -0.07122745211728443 1102 7 -0.08811032176207334 1102 8 -0.0408919230757121 1102 9 -0.2714298017319621 1102 10-11 -0.1881767977193718 1102 12-13 -0.22468055757855865 1102 14-15 -0.19963290767068997 1102 16-17 -0.28453570756564517 1102 18-19 -0.1774941678170805 1102 20-21 -0.24083716650719822 1102 22-23 -0.1436853497188153 1102 24-25 -0.23782948376369006 1102 26-27 -0.28989523846298226 1102 28-29 -0.331249954739512 1102 30-31 -0.19306736433012617 1102 32-33 -0.18591813348675856 1102 34-35 -0.3073571608454344 1102 36-37 -0.25374244525651335 1102 38-39 -0.21349344314837282 1102 40-41 -0.24808938862755525 1102 42-43 -0.28178725761706147 1102 44-45 -0.15111935608797467 1102 46-47 -0.25714166227997737 1102 48-49 -0.11960667283645421 1102 50-51 -0.07160405921545276 1102 52-53 -0.04571914172050384 1102 54-55 -0.07773763788102883 1102 56-57 -0.2497570270534979 1102 58-59 -0.03874414297291651 1102 60-61 -0.2013679620865929 1102 62-63 -0.29184804557699806 1102 64-65 -0.282902078137802 1102 66-67 -0.23838050542763511 1102 68-69 -0.3922695278520649 1102 70-71 -0.3140231909799951 1102 72-73 -0.14423095029409438 1102 74-75 -0.07271781801019017 1102 76-77 -0.10326283907423317 1102 78-79 -0.1406823843390015 1102 80-81 -0.2749862030927801 1102 82-83 -0.27197786252229506 1102 84-85 -0.3706404679083839 1102 86-87 -0.22830575103896678 1102 88-89 -0.006931541864574342 1102 90-91 -0.060924121142900134 1102 92-93 0.07757612537595193 1102 94-95 0.014345223175681099 1102 96-97 0.27411187036645046 1102 98-99 0.49966659443812134 1102 100-101 0.9516443868192574 1103 1 -0.041609560470689644 1103 2 -0.11788551944649583 1103 3 -0.11935229422122262 1103 4 -0.09587228821927596 1103 5 -0.025489603560785667 1103 6 -0.12436377108401331 1103 7 -0.2102105604255584 1103 8 -0.2004047687867896 1103 9 -0.1185054720955705 1103 10-11 -0.16815152746807627 1103 12-13 -0.16411338082719595 1103 14-15 -0.16373791960386797 1103 16-17 -0.17877549417242022 1103 18-19 -0.1572618218954176 1103 20-21 -0.16974633399279782 1103 22-23 -0.20276509140068555 1103 24-25 -0.20113497302383365 1103 26-27 -0.29391601622515395 1103 28-29 -0.2477275624890538 1103 30-31 -0.21453514475972213 1103 32-33 -0.04159551661184224 1103 34-35 -0.002534754555952645 1103 36-37 -0.017273260924064004 1103 38-39 -0.03288695711720635 1103 40-41 -0.13622781344854928 1103 42-43 0.06484618334882697 1103 44-45 -0.05756046236341916 1103 46-47 0.1317639954485017 1103 48-49 0.10235106968777075 1103 50-51 0.1619021881522329 1103 52-53 0.1941559109258506 1103 54-55 0.15422642220600835 1103 56-57 0.12885310912507464 1103 58-59 0.06681812011847654 1103 60-61 0.10119556451735434 1103 62-63 0.16237770200126533 1103 64-65 0.04298097675479795 1103 66-67 0.1094441471064087 1103 68-69 0.08906698766105592 1103 70-71 0.08691321222090664 1103 72-73 -0.044964489533178664 1103 74-75 -0.041912679725758295 1103 76-77 0.0959207914185356 1103 78-79 0.17090613173568414 1103 80-81 0.037999196317585415 1103 82-83 0.031014556402311655 1103 84-85 0.2618156601275565 1103 86-87 0.14933920193028527 1103 88-89 0.2820155309979846 1103 90-91 0.3744107096868099 1103 92-93 0.4504267501127188 1103 94-95 0.30579756067392694 1103 96-97 0.46759284748283036 1103 98-99 0.5821529063853319 1103 100-101 1.3519560532519357 1104 1 -0.06503278750209063 1104 2 0.029984456995968856 1104 3 -0.07571423648766995 1104 4 -0.1307866723829889 1104 5 -0.09090713458993349 1104 6 -0.053793819253904473 1104 7 -0.10009699198563027 1104 8 -0.14428613723374895 1104 9 -0.09300566547836553 1104 10-11 -0.005872774962888627 1104 12-13 -0.04319252713006705 1104 14-15 -0.0188963176911372 1104 16-17 -0.08936179563860946 1104 18-19 -0.06107867081071561 1104 20-21 -0.13906298952530705 1104 22-23 -0.28348394798097587 1104 24-25 -0.2407344948409289 1104 26-27 -0.28161405778487136 1104 28-29 -0.22223830402416667 1104 30-31 -0.26658834260790343 1104 32-33 -0.23137154417768357 1104 34-35 -0.18229724532631764 1104 36-37 -0.21526348630292347 1104 38-39 -0.1889141713501843 1104 40-41 -0.2615505869094008 1104 42-43 -0.21314386622749026 1104 44-45 -0.11473637038353246 1104 46-47 -0.11156781426282691 1104 48-49 -0.014393594705182977 1104 50-51 -0.11796244389305599 1104 52-53 -0.0730493561181973 1104 54-55 0.00472359178514381 1104 56-57 -0.07549202641867936 1104 58-59 -0.2692773054994646 1104 60-61 -0.15013947996068566 1104 62-63 -0.0295336231982688 1104 64-65 0.0088267627679528 1104 66-67 0.14051953123495764 1104 68-69 0.14783267803763067 1104 70-71 0.0524149526660338 1104 72-73 0.1926449475470946 1104 74-75 0.05752211511267902 1104 76-77 0.10421533099168911 1104 78-79 0.01792487228629369 1104 80-81 0.013374886342546688 1104 82-83 -0.004837031094613309 1104 84-85 -0.006524763908164033 1104 86-87 0.12521364375722754 1104 88-89 -0.056409278256261075 1104 90-91 -0.009151049744996698 1104 92-93 0.11460944411082252 1104 94-95 0.3803816118676231 1104 96-97 0.21475609772016568 1104 98-99 0.49763231990806744 1104 100-101 0.9181319964325567 1105 1 -0.058937170940716044 1105 2 0.04164445522280147 1105 3 -0.01862614730312373 1105 4 -0.009958975525783842 1105 5 -0.0891175236283317 1105 6 -0.07886982230832729 1105 7 -0.12104788099971842 1105 8 -0.11025278454811627 1105 9 -0.07047000910655044 1105 10-11 -0.14212844837997807 1105 12-13 -0.07515545794512235 1105 14-15 -0.18019724974740825 1105 16-17 -0.16041213926107645 1105 18-19 -0.18565754382055388 1105 20-21 -0.09837847584476123 1105 22-23 -0.003440576905894943 1105 24-25 -0.17960387254536414 1105 26-27 -0.10689729964761341 1105 28-29 -0.27692260892868603 1105 30-31 -0.24918825480757079 1105 32-33 -0.14936993259833287 1105 34-35 -0.241188708960685 1105 36-37 -0.2554367847067809 1105 38-39 -0.1729263604383462 1105 40-41 -0.1993617847404181 1105 42-43 -0.08972952731821238 1105 44-45 -0.12181813193843283 1105 46-47 -0.1852357230992041 1105 48-49 -0.04034313239498033 1105 50-51 -0.1581731003363629 1105 52-53 -0.0936445393978147 1105 54-55 -0.021052919195618358 1105 56-57 -0.0724880110550501 1105 58-59 -0.046465020889122854 1105 60-61 -0.02676249909085726 1105 62-63 0.02551800596974374 1105 64-65 0.032239349960534014 1105 66-67 0.07164721493641224 1105 68-69 0.14149521732612413 1105 70-71 0.04965385393213495 1105 72-73 0.11491448598047072 1105 74-75 0.2551461438036533 1105 76-77 0.2828902538446556 1105 78-79 0.10570911512999714 1105 80-81 0.106734771887524 1105 82-83 0.06947079057062311 1105 84-85 0.014060945801634972 1105 86-87 -0.029890322415337067 1105 88-89 -0.015126320958110284 1105 90-91 0.14760167506817723 1105 92-93 0.2960424270054851 1105 94-95 0.5581082840063196 1105 96-97 0.5141021354085282 1105 98-99 0.8606886508266491 1105 100-101 1.1941154959763978 1106 1 -0.15747365911838784 1106 2 -0.03868014001796638 1106 3 -0.0710145525381023 1106 4 -0.07426516531945282 1106 5 -0.19649955028804555 1106 6 -0.14318283005655275 1106 7 -0.0771769948777461 1106 8 -0.11391629879460652 1106 9 -0.21277511408789707 1106 10-11 -0.20538618827350064 1106 12-13 -0.26171685286832513 1106 14-15 -0.22779111783059136 1106 16-17 -0.22003969713035332 1106 18-19 -0.18825556328050652 1106 20-21 -0.11100494890171575 1106 22-23 -0.1835114684822301 1106 24-25 -0.200026676633712 1106 26-27 -0.29530408760317073 1106 28-29 -0.3198461781430453 1106 30-31 -0.2920873930181145 1106 32-33 -0.1588387457591054 1106 34-35 -0.12710633746397093 1106 36-37 -0.2725509869606455 1106 38-39 -0.3432699247437725 1106 40-41 -0.29062616683677334 1106 42-43 -0.46850496965195276 1106 44-45 -0.3918271240629352 1106 46-47 -0.5274879510552424 1106 48-49 -0.4152402949138079 1106 50-51 -0.252432647745394 1106 52-53 -0.2556989860677774 1106 54-55 -0.19587341760325216 1106 56-57 -0.21505181953416042 1106 58-59 -0.2667686622828924 1106 60-61 -0.10142932837836582 1106 62-63 0.03085551744663917 1106 64-65 -0.03093922590511511 1106 66-67 -0.033340005186552446 1106 68-69 -0.007357964840522868 1106 70-71 0.003994201678118259 1106 72-73 0.03828067680441194 1106 74-75 -0.03008184859659835 1106 76-77 -0.03987569183594175 1106 78-79 0.08085956466046795 1106 80-81 -0.017539305340207534 1106 82-83 0.014676404928930964 1106 84-85 -0.02494476158387826 1106 86-87 -0.046021413500177744 1106 88-89 0.08979707553473304 1106 90-91 0.15013473457225146 1106 92-93 0.32389320319173365 1106 94-95 0.36166445334612973 1106 96-97 0.3297770295787217 1106 98-99 0.4636471506948361 1106 100-101 1.052568130888563 1107 1 -0.08214027117768552 1107 2 -0.05354519656655299 1107 3 -0.011541939795542078 1107 4 -0.019680044760384874 1107 5 -0.09575495891428432 1107 6 -0.11977664916899755 1107 7 -0.019106459479182547 1107 8 -0.027231638335869945 1107 9 -0.06978400243515637 1107 10-11 0.011089519108288926 1107 12-13 -0.06016274063420468 1107 14-15 -0.00449501739566216 1107 16-17 0.033311163834632396 1107 18-19 0.07738375242263373 1107 20-21 -0.06388532081781761 1107 22-23 0.013337153913845157 1107 24-25 -0.023374720657905357 1107 26-27 -0.10961095035022339 1107 28-29 -0.14402372133114483 1107 30-31 -0.12262662740956642 1107 32-33 -0.1371197326073883 1107 34-35 -0.06151137359473324 1107 36-37 -0.06475145724991904 1107 38-39 -0.08772644496281856 1107 40-41 -0.08507639389019772 1107 42-43 -0.022170888464124516 1107 44-45 0.053729201767218626 1107 46-47 0.061491217567635204 1107 48-49 0.18143531537342028 1107 50-51 0.21835607570094595 1107 52-53 0.26588257635543044 1107 54-55 0.2674032639206132 1107 56-57 0.40747251903415105 1107 58-59 0.29426105943931447 1107 60-61 0.36165576121433674 1107 62-63 0.2787165403757399 1107 64-65 0.28222387896300205 1107 66-67 0.38556866000305234 1107 68-69 0.4576316564307703 1107 70-71 0.37938213038754043 1107 72-73 0.36838678157463534 1107 74-75 0.3471808441191051 1107 76-77 0.4389241315558543 1107 78-79 0.34614086916377573 1107 80-81 0.5626494097152417 1107 82-83 0.568573472713318 1107 84-85 0.6514556675500849 1107 86-87 0.5565214249772374 1107 88-89 0.679369399990378 1107 90-91 0.7908293402305091 1107 92-93 0.9201762398139621 1107 94-95 0.6885782249901276 1107 96-97 0.9722189376665398 1107 98-99 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20-21 0.5150933756699843 2316 22-23 0.414590874121771 2316 24-25 0.6684023020404339 2316 26-27 0.5662593958419819 2316 28-29 0.7793282366943686 2316 30-31 0.6533183256772972 2316 32-33 0.44342489772355265 2316 34-35 0.34464126238038517 2316 36-37 0.681997871693504 2316 38-39 0.529337449354891 2316 40-41 0.3538878677853816 2316 42-43 0.6038912461040056 2316 44-45 0.4907504404038576 2316 46-47 0.7321269169667204 2316 48-49 0.24341337121506257 2316 50-51 0.24664587482998712 2316 52-53 0.4302204807126415 2316 54-55 0.43595483593654194 2316 56-57 0.41721845012008174 2316 58-59 0.7142897668748631 2316 60-61 0.7550652152054056 2316 62-63 0.6786411225365754 2316 64-65 0.46452339320724434 2316 66-67 0.4145696574647957 2316 68-69 0.606534853984968 2316 70-71 0.7653730640279548 2316 72-73 0.9278995661147889 2316 74-75 1.0388865037008195 2316 76-77 0.8880219557917428 2316 78-79 0.8275037425023584 2316 80-81 1.302535715295349 2316 82-83 0.9414311600617715 2316 84-85 0.8363793982191936 2316 86-87 0.9539036961084228 2316 88-89 1.2321107978965173 2316 90-91 1.028827766998166 2316 92-93 1.45532014191674 2316 94-95 1.3692007059937517 2316 96-97 1.595070542931193 2316 98-99 1.6461102631069444 2316 100-101 1.6742348979517736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 6.0 5 27.0 6 52.0 7 136.0 8 312.0 9 604.0 10 961.0 11 1573.0 12 2502.0 13 3368.0 14 4430.0 15 6131.0 16 8421.0 17 11771.0 18 10656.0 19 7083.0 20 6388.0 21 6387.0 22 6893.0 23 7558.0 24 8501.0 25 10204.0 26 12369.0 27 15302.0 28 19063.0 29 23915.0 30 30474.0 31 38662.0 32 49933.0 33 65521.0 34 91070.0 35 133750.0 36 206934.0 37 305268.0 38 219632.0 39 17673.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.218504313686054 19.00768336636353 15.30647544421924 22.467336875731174 2 16.183727935557844 20.23790865945227 38.83529290900815 24.74307049598174 3 19.917557593077657 23.850931770172107 29.47619751083022 26.755313125920015 4 13.6793354815052 16.661105016208776 35.673400888014356 33.98615861427167 5 14.196433325259408 37.197743400691856 33.59132395608285 15.014499317965882 6 33.18430609724365 35.52019928955852 16.548397232322763 14.74709738087507 7 29.396184732782064 32.35245816201575 19.779728646449254 18.471628458752924 8 27.32027682771457 34.62941035756788 18.723365005995333 19.326947808722217 9 26.583814322362258 16.65968023384428 19.57388508905265 37.18262035474081 10-11 25.661851384143763 26.486803466283526 26.230341679089815 21.621003470482897 12-13 26.485124662017878 23.565889031249778 27.14502174971906 22.803964557013288 14-15 23.196281767959945 25.046136702618675 25.462248141596373 26.29533338782501 16-17 22.679385723830688 28.363479978433293 26.14243174355117 22.814702554184848 18-19 22.745563115234148 26.700009073614005 28.57382284855988 21.980604962591965 20-21 23.31742576321467 25.68627642127665 28.88030103401705 22.115996781491624 22-23 23.103529859053854 25.446083252376127 28.941709688655653 22.508677199914363 24-25 23.082325195112944 25.729224666250477 28.904536668860104 22.283913469776476 26-27 22.644435420765284 25.98470898822185 28.927015808061096 22.443839782951773 28-29 22.61853517772215 26.35243264646198 28.81443824682074 22.21459392899513 30-31 22.73002686855704 25.902736273451872 28.73267098053361 22.634565877457483 32-33 22.69464589283987 25.93831292270106 28.66819593307122 22.69884525138785 34-35 23.144802131474417 25.890320253581265 28.564149326190424 22.400728288753893 36-37 23.10014645262936 25.778399849423 28.602993392759256 22.51846030518838 38-39 22.846065878030032 25.9998500215594 28.653118614199208 22.500965486211356 40-41 22.978783076103394 25.946722788199327 28.537244443244624 22.53724969245265 42-43 22.818156489836294 26.00276811886037 28.304221587502294 22.874853803801045 44-45 22.943379813803162 26.01240312402749 28.369896553146333 22.674320509023012 46-47 23.39068957216785 26.475005942842234 27.67635993601977 22.457944548970143 48-49 23.31934671454437 26.516709516387436 27.46021574962515 22.703728019443037 50-51 23.151251146387388 26.771473157475196 27.542242922393605 22.53503277374381 52-53 23.449489384055106 26.71486682216797 27.18830718169319 22.647336612083734 54-55 23.223240150067078 26.681711868512085 27.207794009465054 22.887253971955783 56-57 23.290917312380536 26.960556774952625 27.135092614603117 22.613433298063722 58-59 23.41626066618324 26.538821195062457 27.152677428522793 22.892240710231512 60-61 23.05856530414979 26.591275682639477 27.676734878747272 22.67342413446346 62-63 22.953693823268495 26.68306166233108 27.81658851610416 22.54665599829626 64-65 22.95706830781598 26.78868302866737 27.526307856474926 22.727940807041726 66-67 22.951856603903753 26.808817453134033 27.2783207365075 22.96100520645471 68-69 22.995462443111812 26.594612672914213 27.50613593773551 22.903788946238464 70-71 23.091897712624395 26.626632781842574 27.353721719007424 22.927747786525607 72-73 23.0451798487781 26.61137261283339 27.322376506988554 23.021071031399952 74-75 22.9418831273523 27.17892341944768 26.838025491606153 23.041167961593867 76-77 23.20175593178142 26.81830350413974 27.050543029552237 22.9293975345266 78-79 23.133178906921966 26.797156734308835 27.072102236383394 22.997562122385805 80-81 23.18843405368661 26.980084144741557 26.974272525928257 22.85720927564358 82-83 23.161715218789194 26.903056355956828 26.97684533372827 22.958383091525704 84-85 23.02370696766182 26.97606806585819 26.91937651289958 23.080848453580415 86-87 23.111732182909062 27.071314856655647 26.831913925147933 22.98503903528736 88-89 22.995912374384815 26.946646399762436 26.67882480951035 23.378616416342403 90-91 23.049674509852448 26.868713911765003 26.793158051236247 23.288453527146302 92-93 23.039223222049205 26.76724595306292 26.91381419765978 23.279716627228098 94-95 22.93415930716583 26.74526466082306 26.896666534186902 23.42390949782421 96-97 22.950506810084757 26.661502455499924 26.99895091024846 23.389039824166858 98-99 22.771739313831027 26.857010448548575 26.670326114547464 23.70092412307293 100-101 22.783225740649634 26.586704604086925 26.953579656583354 23.67648999868009 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 250.0 1 186.5 2 153.5 3 200.0 4 187.0 5 192.5 6 328.0 7 706.0 8 1206.0 9 1510.0 10 1491.0 11 1384.0 12 1430.0 13 1523.5 14 1637.5 15 1735.0 16 1800.5 17 1844.0 18 1906.0 19 2021.0 20 2215.5 21 2522.5 22 2885.0 23 3377.5 24 3994.5 25 4898.0 26 6276.5 27 7877.0 28 9272.5 29 10818.5 30 12793.0 31 14734.5 32 17167.5 33 19109.5 34 20643.5 35 22734.5 36 25585.5 37 28514.5 38 31105.0 39 33184.5 40 34816.5 41 37641.0 42 39775.5 43 41395.5 44 44625.5 45 47730.5 46 49372.0 47 51863.0 48 55734.0 49 55924.5 50 53239.5 51 51106.5 52 50485.0 53 51117.5 54 51527.5 55 48498.5 56 42816.0 57 39011.0 58 36399.0 59 32510.5 60 26935.0 61 22148.0 62 18005.0 63 13424.0 64 9524.0 65 7002.5 66 5143.0 67 3894.0 68 2987.0 69 2149.5 70 1565.5 71 1072.0 72 749.0 73 613.0 74 472.0 75 338.0 76 229.5 77 144.5 78 92.5 79 63.0 80 39.0 81 22.0 82 17.0 83 17.0 84 10.5 85 4.5 86 3.0 87 2.5 88 2.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06883948476870158 2 0.0 3 0.0 4 0.0 5 0.001949702182991548 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.7494272749837465E-4 12-13 0.005361681003226757 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010123453642456115 24-25 0.0010873339097452865 26-27 6.374026367472369E-4 28-29 0.003824415820483421 30-31 5.999083639973994E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.998625459960992E-4 40-41 1.8747136374918733E-4 42-43 0.010535890642704326 44-45 1.4997709099934985E-4 46-47 0.0 48-49 3.749427274983746E-5 50-51 0.0 52-53 3.749427274983746E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.124828182495124E-4 82-83 3.374484547485371E-4 84-85 3.7494272749837465E-4 86-87 0.0 88-89 0.0 90-91 0.006486509185721881 92-93 0.002137173546740735 94-95 0.0 96-97 0.0 98-99 1.8747136374918733E-4 100-101 0.008473705641463266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1333537.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.4938894057568 #Duplication Level Percentage of deduplicated Percentage of total 1 78.22603206691413 44.97518836306346 2 12.11950996417115 13.935955310640471 3 3.8249543317901264 6.597345040020358 4 1.7850213215824153 4.1051127379990895 5 1.0085016686759136 2.899134170218708 6 0.6357003369074403 2.192933091921523 7 0.4451641245623443 1.7915951861498574 8 0.29592281086522565 1.3611002688420775 9 0.24430179558022214 1.2641274375045386 >10 1.3207189856844368 13.595073968853514 >50 0.06388950417105643 2.442295345779372 >100 0.028064087204369304 3.0304677876590462 >500 0.0015663542760886585 0.5873139058674842 >1k 5.221180920295527E-4 0.8301643785722093 >5k 1.3052952300738818E-4 0.3921930069081871 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5226 0.39189013878130113 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4691 0.3517712669389751 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3161 0.23703879232447245 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1911 0.14330311044987878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.1248281824951239E-4 14-15 0.0 0.0 0.0 0.0 1.4997709099934985E-4 16-17 0.0 0.0 0.0 0.0 1.4997709099934985E-4 18-19 0.0 0.0 0.0 0.0 1.4997709099934985E-4 20-21 0.0 0.0 0.0 0.0 1.8747136374918733E-4 22-23 0.0 0.0 0.0 0.0 2.2496563649902478E-4 24-25 0.0 0.0 0.0 7.498854549967493E-5 2.2496563649902478E-4 26-27 0.0 0.0 0.0 1.4997709099934985E-4 2.2496563649902478E-4 28-29 0.0 0.0 0.0 4.124370002482121E-4 2.2496563649902478E-4 30-31 0.0 0.0 0.0 7.498854549967493E-4 2.2496563649902478E-4 32-33 0.0 0.0 0.0 0.0010123453642456115 2.2496563649902478E-4 34-35 0.0 0.0 0.0 0.0014247823644938234 2.2496563649902478E-4 36-37 0.0 0.0 0.0 0.002324644910489923 2.2496563649902478E-4 38-39 0.0 0.0 0.0 0.0036369444567342342 2.2496563649902478E-4 40-41 0.0 0.0 0.0 0.0058866008217244815 2.2496563649902478E-4 42-43 0.0 0.0 0.0 0.009073614005460666 2.2496563649902478E-4 44-45 0.0 0.0 0.0 0.016160031555179945 2.2496563649902478E-4 46-47 0.0 0.0 0.0 0.031757649019112336 2.2496563649902478E-4 48-49 0.0 0.0 0.0 0.04645540393704862 2.2496563649902478E-4 50-51 0.0 0.0 0.0 0.06246545840122921 2.2496563649902478E-4 52-53 0.0 0.0 0.0 0.08556193041512908 2.999541819986997E-4 54-55 0.0 0.0 0.0 0.11338268079550848 2.999541819986997E-4 56-57 0.0 0.0 0.0 0.14671508927011398 2.999541819986997E-4 58-59 0.0 0.0 0.0 0.1912582852969209 2.999541819986997E-4 60-61 0.0 0.0 0.0 0.24607491205718326 2.999541819986997E-4 62-63 0.0 0.0 0.0 0.31851384700986923 2.999541819986997E-4 64-65 0.0 0.0 0.0 0.38821570005181705 2.999541819986997E-4 66-67 0.0 0.0 0.0 0.4786143916516752 2.999541819986997E-4 68-69 0.0 0.0 0.0 0.5912846812649368 2.999541819986997E-4 70-71 0.0 0.0 0.0 0.6939814943267416 2.999541819986997E-4 72-73 0.0 0.0 0.0 0.8272736339524138 3.7494272749837465E-4 74-75 0.0 0.0 0.0 0.9561039551208553 4.4993127299804955E-4 76-77 0.0 0.0 0.0 1.10034442238948 4.4993127299804955E-4 78-79 0.0 0.0 0.0 1.2744303307669753 5.249198184977245E-4 80-81 0.0 0.0 0.0 1.4468289968707282 5.249198184977245E-4 82-83 0.0 0.0 0.0 1.6545472679048276 5.249198184977245E-4 84-85 0.0 0.0 0.0 1.9045965728734935 5.249198184977245E-4 86-87 0.0 0.0 0.0 2.1887656660445116 5.249198184977245E-4 88-89 0.0 0.0 0.0 2.5407618986199854 5.249198184977245E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7570 0.0 29.447834 1 TACCGTG 150 3.3358447E-8 28.501451 7 GGTATCA 5280 0.0 26.826174 1 TATTCCG 185 8.976713E-9 25.674095 5 ATCAACG 9060 0.0 24.37764 3 TCAACGC 9135 0.0 24.229488 4 TATCAAC 9170 0.0 24.085213 2 CAACGCA 9205 0.0 24.045233 5 AACGCAG 9320 0.0 23.850462 6 GTACCGT 125 2.2571077E-4 22.798595 6 TACATGG 5575 0.0 21.895512 2 GTACATG 5680 0.0 21.840801 1 GAGTACT 5070 0.0 21.408882 12-13 ACGCAGA 10460 0.0 21.117233 7 TGTACCG 180 5.172533E-6 21.10981 5 AGAGTAC 8110 0.0 21.02758 10-11 ACATGGG 5475 0.0 20.994143 3 CGCAGAG 10555 0.0 20.882162 8 GTAAGGT 485 0.0 20.565742 4 AGTACTT 5340 0.0 20.548798 12-13 >>END_MODULE