FastQCFastQC Report
Thu 26 May 2016
SRR937175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619784
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52480.846746608495863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44470.7175080350573748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.4682276405973694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15570.2512165528635783No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA10300.16618692964000362No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.16215326629922683No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9900.15973306829476078No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT9240.14908419707511003No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.14601861293611967No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC7930.12794780116943966No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7630.12310740516050753No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT7580.12230067249235219No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG7410.11955778142062395No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG7140.11520142501258504No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC6980.1126198804744879No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6880.11100641513817718No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG6770.10923160326823539No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA6730.10858621713371108No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC6700.10810217753281788No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA6700.10810217753281788No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA6570.10600467259561397No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT6430.10374582112477897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.054.2234761
GTATGGT957.2759576E-1250.027951
ACAATGC704.3071814E-640.7139748
CGAGGGG250.001616566637.99663586-87
GTGGTAC1152.4992914E-937.1736266
GTATCAA37300.035.0396841
TGGTACT1255.644324E-934.1997387
CATTCCG1553.6379788E-1133.709429
GTACCAC1401.7007551E-830.5404223
GGATATG3200.029.7040961
GTTATAT804.4765865E-429.7040961
GGTACTC1503.331661E-828.4997838
ACTCCCG1354.2200736E-728.1479326
TCAACGC46550.027.9634934
ATCAACG46600.027.9334893
CAACGCA47150.027.6098825
GATTGGC3500.027.1426499
AACGCAG48450.026.9605796
CGCAGAA1257.3748306E-626.6041032
ACTGTCC908.94325E-426.3886858
Produced by FastQC (version 0.11.5)