##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937175_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 619784 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.911795076994565 33.0 31.0 34.0 30.0 34.0 2 32.06924509183845 33.0 31.0 34.0 30.0 34.0 3 32.06620693661017 34.0 31.0 34.0 30.0 34.0 4 35.725865785499465 37.0 35.0 37.0 35.0 37.0 5 35.74554199527577 37.0 35.0 37.0 35.0 37.0 6 35.801329172744055 37.0 35.0 37.0 35.0 37.0 7 35.75737676351761 37.0 35.0 37.0 35.0 37.0 8 35.75143114375331 37.0 35.0 37.0 35.0 37.0 9 37.38466627082984 39.0 37.0 39.0 35.0 39.0 10-11 37.29889768048223 39.0 37.0 39.0 35.0 39.0 12-13 37.20675348185819 39.0 37.0 39.0 34.0 39.0 14-15 38.346866004930746 40.0 38.0 41.0 34.0 41.0 16-17 38.270006163437586 40.0 38.0 41.0 34.0 41.0 18-19 38.28888854826843 40.0 38.0 41.0 34.0 41.0 20-21 38.21303066874911 40.0 38.0 41.0 34.0 41.0 22-23 38.265327113962286 40.0 38.0 41.0 34.0 41.0 24-25 38.244039052315 40.0 38.0 41.0 34.0 41.0 26-27 38.15233855665845 40.0 38.0 41.0 34.0 41.0 28-29 38.051711564028764 40.0 38.0 41.0 34.0 41.0 30-31 37.77687952577027 40.0 37.0 41.0 33.0 41.0 32-33 37.510577878744854 40.0 36.5 41.0 33.0 41.0 34-35 37.46961925444994 40.0 37.0 41.0 33.0 41.0 36-37 37.31353261781524 40.0 36.0 41.0 33.0 41.0 38-39 37.179847333909876 40.0 36.0 41.0 33.0 41.0 40-41 36.97898138706388 39.5 36.0 41.0 31.5 41.0 42-43 36.70401059078647 39.0 36.0 41.0 31.0 41.0 44-45 36.38636605656164 39.0 35.5 41.0 30.5 41.0 46-47 35.90920788532779 39.0 35.0 41.0 29.5 41.0 48-49 35.53179817484801 39.0 35.0 40.0 27.5 41.0 50-51 34.58573551430821 38.0 34.0 39.5 25.5 40.5 52-53 34.71442389606702 38.0 35.0 39.5 26.5 40.5 54-55 35.07787793812038 39.0 35.0 40.0 26.0 41.0 56-57 34.87862545661069 39.0 35.0 40.0 25.0 41.0 58-59 34.68159310340377 38.5 35.0 40.0 24.0 41.0 60-61 34.419404179520605 38.0 34.5 40.0 23.0 41.0 62-63 34.08367753927175 38.0 34.0 40.0 22.0 41.0 64-65 33.72824645360319 38.0 34.0 40.0 21.0 41.0 66-67 33.31745172511714 37.0 33.0 39.5 18.5 41.0 68-69 32.844776567320224 36.5 33.0 39.0 16.0 40.5 70-71 32.42880906896596 36.0 32.0 39.0 12.0 40.0 72-73 31.939123146128328 36.0 31.5 38.5 8.0 40.0 74-75 31.375501787719593 35.0 31.0 37.5 4.5 39.5 76-77 30.868746208356463 35.0 31.0 37.0 2.0 39.0 78-79 30.336297000245246 34.5 30.0 36.5 2.0 39.0 80-81 29.747387799620512 34.0 29.5 36.0 2.0 38.0 82-83 29.18351151368864 34.0 29.0 35.5 2.0 37.0 84-85 28.6030181482581 34.0 28.5 35.0 2.0 37.0 86-87 28.052482800459515 34.0 27.5 35.0 2.0 36.0 88-89 27.484867308610742 33.0 26.0 35.0 2.0 36.0 90-91 26.939089263356266 33.0 26.0 35.0 2.0 35.5 92-93 26.298441876524723 32.5 25.0 35.0 2.0 35.0 94-95 25.542250203296632 32.0 21.5 34.5 2.0 35.0 96-97 24.70531265731287 32.0 14.5 34.0 2.0 35.0 98-99 23.573381210228078 31.0 2.0 34.0 2.0 35.0 100-101 20.32209447162237 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3150194473999548 1101 2 0.3822574808648298 1101 3 0.5279679529579866 1101 4 0.2626277358630631 1101 5 0.40589905086618217 1101 6 0.2750187942034401 1101 7 0.3694251240214541 1101 8 0.22792397547800647 1101 9 0.4120009451226778 1101 10-11 0.46873662286094486 1101 12-13 0.5516204552671198 1101 14-15 0.6209024583306473 1101 16-17 0.7037517705959431 1101 18-19 0.790668274529196 1101 20-21 0.741369078612685 1101 22-23 0.7791093400409963 1101 24-25 0.610957244454795 1101 26-27 0.5870565790387943 1101 28-29 0.48279320211498344 1101 30-31 0.5477575721802026 1101 32-33 0.6243453777007844 1101 34-35 0.48195373841455336 1101 36-37 0.5951119855767075 1101 38-39 0.5599130291589347 1101 40-41 0.6718426961112911 1101 42-43 0.7641732262619811 1101 44-45 0.5787960167751081 1101 46-47 0.7833657190323109 1101 48-49 0.6680358368881372 1101 50-51 0.5503483493819843 1101 52-53 0.20678369252936335 1101 54-55 0.395708979116435 1101 56-57 0.5471940653921266 1101 58-59 0.6244586803865673 1101 60-61 0.6015715063479803 1101 62-63 0.9789777194124056 1101 64-65 0.7039358126487443 1101 66-67 0.8292283385143904 1101 68-69 0.8692404317077731 1101 70-71 0.9141600237929524 1101 72-73 0.8530387723557133 1101 74-75 0.7729775306387516 1101 76-77 0.9058225536888536 1101 78-79 1.162498040690135 1101 80-81 1.3204372699009745 1101 82-83 1.1191642178022825 1101 84-85 1.090304746660447 1101 86-87 1.2601326997655669 1101 88-89 1.598433837734234 1101 90-91 1.7611926036586993 1101 92-93 1.8917588344305152 1101 94-95 1.881074310228005 1101 96-97 1.8706195811279684 1101 98-99 2.1290673814282997 1101 100-101 2.2052538765661858 1102 1 0.4815985054319185 1102 2 0.5366748675151882 1102 3 0.30046585035075424 1102 4 0.2401489530815013 1102 5 0.31919517442302947 1102 6 0.2244319756393267 1102 7 0.08249091651323681 1102 8 0.2422455979262068 1102 9 0.4347043245859368 1102 10-11 0.4062461801665549 1102 12-13 0.44951737016886995 1102 14-15 0.61158647469275 1102 16-17 0.8390808286278997 1102 18-19 0.6194180833830885 1102 20-21 0.6970781351154827 1102 22-23 0.4813897131582152 1102 24-25 0.5977705520471162 1102 26-27 0.727118892671335 1102 28-29 0.7830861335317607 1102 30-31 0.9084885149128255 1102 32-33 0.8103592166793021 1102 34-35 0.976642265824907 1102 36-37 1.135104530878337 1102 38-39 1.0712503829321562 1102 40-41 1.127566088572486 1102 42-43 0.8881673198471205 1102 44-45 0.9090009254072626 1102 46-47 1.1125097667423773 1102 48-49 1.2055387040798209 1102 50-51 1.0522144133012503 1102 52-53 1.0483187869555408 1102 54-55 1.1317146943851952 1102 56-57 1.407052903988351 1102 58-59 1.277831931399632 1102 60-61 1.182987803465501 1102 62-63 1.3133018267600605 1102 64-65 1.213550462086772 1102 66-67 1.3555587154545279 1102 68-69 1.3436626925839832 1102 70-71 1.4451734804792409 1102 72-73 1.6498361192476843 1102 74-75 1.5528417213261072 1102 76-77 1.758133510183999 1102 78-79 1.7773171208950416 1102 80-81 1.9242487233760066 1102 82-83 1.9707870482939107 1102 84-85 2.0304975175144904 1102 86-87 2.104680734783532 1102 88-89 2.090623129729039 1102 90-91 2.2052728085673294 1102 92-93 2.1151990821558755 1102 94-95 2.0920422741396187 1102 96-97 2.2383775328062328 1102 98-99 2.343554823128734 1102 100-101 2.4774698334436245 1103 1 0.25666807178608053 1103 2 0.12740526754506476 1103 3 0.12745572821278017 1103 4 0.3196133388432756 1103 5 0.19395706139872004 1103 6 0.2826949644713608 1103 7 0.26857157613300586 1103 8 0.20545713566945523 1103 9 0.2944580877055003 1103 10-11 0.26787683418366015 1103 12-13 0.2680834161853838 1103 14-15 0.3609339983173001 1103 16-17 0.51714618364975 1103 18-19 0.40534396953705 1103 20-21 0.32144199982993626 1103 22-23 0.12494385352351145 1103 24-25 0.36421085571125644 1103 26-27 0.39190711214617124 1103 28-29 0.13375308344398462 1103 30-31 0.44137612556534833 1103 32-33 0.45796594734078866 1103 34-35 0.625669504087341 1103 36-37 0.6333140143131359 1103 38-39 0.8043455254264629 1103 40-41 0.8476397168547152 1103 42-43 0.7551636874562817 1103 44-45 0.9469869561098392 1103 46-47 0.7548033087436465 1103 48-49 0.7966752969158861 1103 50-51 0.7208743038626366 1103 52-53 0.6111316021051323 1103 54-55 1.1124263721949248 1103 56-57 0.877423006330261 1103 58-59 1.010131029371479 1103 60-61 0.9497199123064988 1103 62-63 0.7184067832460741 1103 64-65 0.6983299040619002 1103 66-67 0.7342471328900047 1103 68-69 0.8480829574030153 1103 70-71 0.9778976802327222 1103 72-73 1.2370345094168762 1103 74-75 0.8712303497950948 1103 76-77 0.7672518383504361 1103 78-79 0.746496658115376 1103 80-81 0.9650267460107536 1103 82-83 1.0673068630326412 1103 84-85 1.289981185762354 1103 86-87 1.100132075686684 1103 88-89 1.303737788153569 1103 90-91 1.4944960932197802 1103 92-93 1.6752922730571562 1103 94-95 1.9615936878773397 1103 96-97 1.7786711588473914 1103 98-99 1.9211855051591407 1103 100-101 2.318926244753527 1104 1 0.21510655095070774 1104 2 0.27976641247224165 1104 3 0.2829251697433506 1104 4 0.30645200095596437 1104 5 0.2207644129980295 1104 6 0.25525048033254905 1104 7 0.3050360366524032 1104 8 0.3301052906018853 1104 9 0.320723876646241 1104 10-11 0.3569832330422784 1104 12-13 0.25491525187799624 1104 14-15 0.5298594515347048 1104 16-17 0.691877507133313 1104 18-19 0.39303707189466053 1104 20-21 0.397145860625578 1104 22-23 0.24186791415122144 1104 24-25 0.39184989282194493 1104 26-27 0.43176771664843017 1104 28-29 0.21683857980436727 1104 30-31 0.16302770963613256 1104 32-33 0.3088325613681704 1104 34-35 0.30212449863758195 1104 36-37 0.3891295553410643 1104 38-39 0.5630338515655069 1104 40-41 0.6317575954764152 1104 42-43 0.5310466280049937 1104 44-45 0.42451210577909393 1104 46-47 0.6280617183243038 1104 48-49 0.8973627174165344 1104 50-51 0.8408258817896339 1104 52-53 0.8366867607170079 1104 54-55 1.0696144787507933 1104 56-57 0.9149387355398275 1104 58-59 1.3004905314068793 1104 60-61 1.126527605226812 1104 62-63 1.0163379228713936 1104 64-65 1.110263396526669 1104 66-67 1.3583719289507101 1104 68-69 1.0662633068702192 1104 70-71 1.2684665258168266 1104 72-73 1.4913471002935985 1104 74-75 1.4352428284909742 1104 76-77 1.3944769668577983 1104 78-79 1.6089852523051853 1104 80-81 1.853333998626976 1104 82-83 1.639203856904789 1104 84-85 1.4232522469748616 1104 86-87 1.4781302962665173 1104 88-89 1.5719080117643358 1104 90-91 1.699741514398518 1104 92-93 2.051011607071615 1104 94-95 2.332066918071053 1104 96-97 2.292463940590814 1104 98-99 2.30577463431273 1104 100-101 2.24393460711811 1105 1 0.031539685071990675 1105 2 0.03830477519865383 1105 3 -0.005651467081627004 1105 4 0.004274819671536534 1105 5 -0.13689161030342945 1105 6 0.07074919249338052 1105 7 0.19258152680814788 1105 8 0.1394109953901932 1105 9 0.19678494888997022 1105 10-11 0.2443263015789583 1105 12-13 0.07099825130683968 1105 14-15 0.36541541071775185 1105 16-17 0.12093474759051048 1105 18-19 0.20885309362052595 1105 20-21 0.3689873721570436 1105 22-23 0.3360585231010944 1105 24-25 0.31639258408849713 1105 26-27 0.24334163774119588 1105 28-29 -0.11169389628252446 1105 30-31 0.18146886621854463 1105 32-33 0.25689555059933866 1105 34-35 0.3564949455188611 1105 36-37 0.2695629345566246 1105 38-39 0.5249293494898595 1105 40-41 0.4470134016943703 1105 42-43 0.3747071111470959 1105 44-45 0.2887200245128341 1105 46-47 0.5044554185258505 1105 48-49 0.5127156860116386 1105 50-51 0.43581632967755723 1105 52-53 0.5417475200278474 1105 54-55 0.7648473949132253 1105 56-57 0.7634649967359337 1105 58-59 0.7003159017290344 1105 60-61 1.0020601929191386 1105 62-63 1.080718939443706 1105 64-65 0.9909670359579579 1105 66-67 0.8950671388892815 1105 68-69 0.9350284474602333 1105 70-71 1.0900017789095031 1105 72-73 1.2756914840237137 1105 74-75 1.4505448087214923 1105 76-77 1.1378840400162495 1105 78-79 1.3096649512768828 1105 80-81 0.9927587273273026 1105 82-83 1.1184890131633374 1105 84-85 0.9339412026324894 1105 86-87 1.218483538404353 1105 88-89 1.1555376830535096 1105 90-91 1.1957762390066407 1105 92-93 1.1392346078572295 1105 94-95 1.2016187675481476 1105 96-97 1.3006406798301882 1105 98-99 1.0868365053012248 1105 100-101 1.5281159593820561 1106 1 -0.11478732104244571 1106 2 -0.646261400701988 1106 3 -0.32273131041342396 1106 4 -0.08549478323202209 1106 5 -0.23385171827499818 1106 6 -0.4456106884096016 1106 7 -0.262139265469699 1106 8 -0.2671603146313686 1106 9 0.03387464097950499 1106 10-11 -0.4975473333877787 1106 12-13 -0.4525794923245243 1106 14-15 -0.3173405999882064 1106 16-17 -0.35066800094243433 1106 18-19 -0.032235148513009904 1106 20-21 -0.07222215560414469 1106 22-23 -0.08909091811869274 1106 24-25 -0.1308903442754712 1106 26-27 0.013015215256849899 1106 28-29 -0.029940087868219223 1106 30-31 -0.013979958997914821 1106 32-33 -0.05270995193808403 1106 34-35 0.005872330764873368 1106 36-37 -0.022115321118945985 1106 38-39 0.19953277740470554 1106 40-41 0.10154998572570406 1106 42-43 -0.0553382113769203 1106 44-45 0.07878873783646867 1106 46-47 -0.03806978471877187 1106 48-49 -0.05652427258709736 1106 50-51 -0.03764673976074562 1106 52-53 0.028539469580323384 1106 54-55 -0.17115049854692188 1106 56-57 -0.21264948595350575 1106 58-59 -0.2732964949388048 1106 60-61 -0.110221344763751 1106 62-63 0.23194752637495242 1106 64-65 0.23682691002153433 1106 66-67 0.456866953769115 1106 68-69 -0.03205230779357038 1106 70-71 0.027830355809605578 1106 72-73 0.2702723874648534 1106 74-75 0.20690660466070554 1106 76-77 0.37864057051776356 1106 78-79 0.4082471405280579 1106 80-81 0.3188722980947283 1106 82-83 0.5676778585332833 1106 84-85 0.30342236460351657 1106 86-87 0.3045245201795943 1106 88-89 0.5741828405196436 1106 90-91 0.37406953136427745 1106 92-93 0.8875977078449218 1106 94-95 0.8550302677966464 1106 96-97 0.6287889765878489 1106 98-99 0.7581205370908286 1106 100-101 0.9930582575167186 1107 1 0.06064515086869804 1107 2 0.043378565857807416 1107 3 0.006480180523730894 1107 4 0.0274761586977732 1107 5 -0.14566695194304202 1107 6 0.004440298719586622 1107 7 0.09871639961376388 1107 8 -0.1477585636139267 1107 9 0.06301998645175644 1107 10-11 -0.01640797775484515 1107 12-13 0.05539011750910561 1107 14-15 0.07235265041830274 1107 16-17 0.08810150967298824 1107 18-19 0.1205195344940364 1107 20-21 -0.02437234255695131 1107 22-23 0.1016491761386007 1107 24-25 0.020006294248752 1107 26-27 0.18091675038284194 1107 28-29 0.24686490243963277 1107 30-31 0.11493709275172392 1107 32-33 0.10290644111297098 1107 34-35 0.20455217752788712 1107 36-37 0.12993426960598953 1107 38-39 0.12204152789850298 1107 40-41 -0.09906369719865893 1107 42-43 -0.19327447287199107 1107 44-45 -0.3563243984691198 1107 46-47 0.11769359825428438 1107 48-49 -0.18848677178325346 1107 50-51 0.013921458969626599 1107 52-53 -0.22556935808317746 1107 54-55 -0.13301580065714802 1107 56-57 -0.12174540606448403 1107 58-59 0.04912589897581654 1107 60-61 0.14507029242836467 1107 62-63 0.2640418571574017 1107 64-65 0.1373399967180262 1107 66-67 0.37083880519904966 1107 68-69 0.3669288683095431 1107 70-71 0.11622182671938219 1107 72-73 0.32914874137983574 1107 74-75 0.5047012964871449 1107 76-77 0.30453468265269734 1107 78-79 0.3311072888041373 1107 80-81 0.2599040754784916 1107 82-83 0.24428165638539312 1107 84-85 0.3403535825068893 1107 86-87 0.2802321388509874 1107 88-89 0.47269548624636926 1107 90-91 0.6063457621752057 1107 92-93 0.7584555777772124 1107 94-95 0.9349169936880024 1107 96-97 1.0976202229705194 1107 98-99 1.0695829949460638 1107 100-101 0.5941965107252365 1108 1 0.09391835022127282 1108 2 0.08077824888990648 1108 3 0.09536607208023895 1108 4 0.17872295529879523 1108 5 0.058524442715715 1108 6 0.0799267095639351 1108 7 0.20156658170170516 1108 8 0.17316300369117243 1108 9 0.33284734010700134 1108 10-11 0.20764238304903415 1108 12-13 0.16133832360568334 1108 14-15 0.10209368090118431 1108 16-17 0.19287794614452736 1108 18-19 0.09973959114361719 1108 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86-87 0.3973473054943035 2316 88-89 0.20803061952281965 2316 90-91 0.39478133991873676 2316 92-93 0.5946427124290885 2316 94-95 0.3654642358270479 2316 96-97 0.7768111400500288 2316 98-99 0.6104637784307343 2316 100-101 1.2421314580641614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2435.0 3 290.0 4 265.0 5 282.0 6 322.0 7 458.0 8 490.0 9 583.0 10 760.0 11 1187.0 12 1814.0 13 2623.0 14 3085.0 15 4501.0 16 6402.0 17 9139.0 18 7182.0 19 3959.0 20 3568.0 21 3693.0 22 4006.0 23 4524.0 24 5178.0 25 5775.0 26 7042.0 27 8265.0 28 9853.0 29 11820.0 30 14934.0 31 18609.0 32 24187.0 33 31669.0 34 43337.0 35 61483.0 36 87322.0 37 124913.0 38 94307.0 39 9522.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.21617755569651 25.043851700345638 16.87481391344872 20.865156830509132 2 14.041080934195776 23.381744718500656 41.50633327025134 21.070841077052226 3 15.280188274874847 29.396921932243693 33.815720305057056 21.507169487824406 4 10.422294182536936 20.75789092297191 42.76254282303694 26.057272071454218 5 9.949549438800185 40.17281622182072 38.009863658935444 11.867770680443654 6 24.157558646272463 43.49211123346771 19.433515898003108 12.91681422225672 7 22.775836390950207 36.36168117451298 25.37334189304351 15.489140541493304 8 22.511962148028246 37.01951297604013 22.608500199706018 17.860024676225606 9 24.219455001212907 17.64987466645104 24.991994824937333 33.13867550739872 10-11 20.658805871902093 29.5975015384914 31.220013569453688 18.523679020152823 12-13 21.80902076402692 29.086214620372004 30.455550362347555 18.649214253253525 14-15 20.32007645740871 30.392062530775803 28.839870404045385 20.447990607770098 16-17 17.110533302933725 32.877177493293495 30.773864097576276 19.23842510619651 18-19 16.91414546045446 31.204964505178502 34.28296094963301 17.597929084734034 20-21 17.33947119924457 30.507505788609052 34.58548385010413 17.567539162042248 22-23 18.10480174238394 30.14982302902518 33.84365758339626 17.90171764519462 24-25 17.702097109450857 30.998477637111698 33.98329524057457 17.316130012862875 26-27 17.19901918538706 30.963327784654116 34.30401534119527 17.533637688763562 28-29 17.071386007084506 29.832476675374743 35.147071070672865 17.949066246867883 30-31 17.92929680898462 30.034907668932657 34.37980869918193 17.65598682290079 32-33 17.394471823441183 30.858496630294603 34.07546361243738 17.67156793382683 34-35 17.862557275887244 30.4241589227484 34.162747205033725 17.550536596330623 36-37 18.39162795360577 29.898421671337026 33.28428511138527 18.425665263671924 38-39 17.114348952902013 31.415607281263792 33.39541711733997 18.074626648494228 40-41 17.240175096096717 30.53567603180185 34.449954932560516 17.77419393954092 42-43 17.545238314681775 30.36121122747786 33.639988551969694 18.45356190587067 44-45 17.54679274081931 30.87253747946552 33.19155919750611 18.389110582209057 46-47 18.1794294021149 30.863577673156577 32.537921142780455 18.419071781948073 48-49 17.754814922621648 31.02524296964699 32.387975225990076 18.831966881741298 50-51 17.639181901763845 30.93590625816202 32.234713622760424 19.19019821731371 52-53 17.787291649313374 31.674480028013264 32.28682543546314 18.251402887210215 54-55 18.513897794189585 31.11145968681551 31.725231427765188 18.649411091229716 56-57 18.068012517006363 31.836962031046767 31.471572798550085 18.623452653396786 58-59 17.76018209270821 30.63721131742393 32.462260704251314 19.140345885616554 60-61 18.471166134120743 31.454589985442215 31.540675704185222 18.53356817625182 62-63 18.04773237277942 31.168072145014325 32.370273486254554 18.413921995951704 64-65 18.15012577030269 30.658047036153302 32.13309989847847 19.058727295065538 66-67 17.95218344794249 31.084315533953117 31.498104846564917 19.465396171539474 68-69 17.928952852410568 30.488305658260405 32.13894821250447 19.443793276824557 70-71 18.165768865148703 30.650410315771776 32.50095384675078 18.682866972328743 72-73 18.34973925935267 30.152467194210757 32.13621405065103 19.36157949578555 74-75 17.633539343327982 31.12478223436635 31.63432350171181 19.60735492059386 76-77 17.63023772487816 30.90649789957409 32.29231008042739 19.17095429512035 78-79 17.756029332104426 30.504014164786923 31.871498217274247 19.868458285834404 80-81 17.667435742799814 30.734885390409882 31.505713098492944 20.091965768297356 82-83 18.08181616825618 30.659398932635767 31.66459994858803 19.59418495052002 84-85 17.69551150191274 30.23999599104127 31.573459723301024 20.491032783744966 86-87 17.433216372567326 30.669424580513382 31.832889173409896 20.064469873509395 88-89 18.427749421604116 29.721707104528384 31.792857477757426 20.057685996110074 90-91 17.99408765691273 29.45105098890688 32.28879783165585 20.26606352252454 92-93 18.360805490658926 29.640261598531943 32.00960380271785 19.989329108091283 94-95 18.189380117488525 28.822600737071934 32.22163570474117 20.766383440698366 96-97 18.649865233904467 28.63484451371833 31.513115239159294 21.20217501321791 98-99 18.18165649091944 29.00936837067315 31.57451363377658 21.23446150463083 100-101 18.598737235546555 28.29547815220264 32.35391354323364 20.751871069017167 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1446.0 1 882.0 2 375.0 3 433.0 4 386.0 5 392.5 6 647.0 7 1267.0 8 1955.0 9 2162.5 10 1808.5 11 1404.5 12 1325.5 13 1334.0 14 1287.0 15 1241.0 16 1283.0 17 1354.0 18 1384.0 19 1521.0 20 1754.0 21 1968.0 22 2262.5 23 2628.5 24 2904.0 25 3722.5 26 5909.0 27 8212.0 28 8794.0 29 9807.0 30 12958.0 31 16018.0 32 19849.5 33 25326.5 34 30882.0 35 34971.0 36 39305.5 37 43049.0 38 41145.0 39 36813.0 40 32834.0 41 28827.0 42 25454.5 43 23638.5 44 22445.0 45 21246.5 46 18640.5 47 14412.0 48 11847.5 49 10020.0 50 7933.0 51 5692.0 52 4090.0 53 3503.0 54 3139.5 55 2705.5 56 2318.5 57 1981.0 58 1674.5 59 1358.0 60 1055.5 61 849.5 62 656.5 63 468.5 64 325.5 65 238.0 66 166.5 67 137.0 68 114.0 69 93.0 70 94.5 71 89.5 72 66.5 73 51.5 74 42.0 75 35.5 76 28.5 77 15.5 78 11.5 79 13.5 80 11.0 81 6.0 82 2.0 83 1.0 84 0.5 85 0.5 86 1.0 87 1.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28913298826688005 2 0.24863500832548116 3 0.24831231525821898 4 0.2487963548591122 5 0.2508938597963161 6 0.22152879067546113 7 0.22314225601177184 8 0.22185148374272326 9 0.2313709292269565 10-11 0.23935758264169452 12-13 0.234759206433209 14-15 0.2259658203503156 16-17 0.21862455307010187 18-19 0.21192867192441237 20-21 0.2142681966620629 22-23 0.2131387709266454 24-25 0.21580098873155812 26-27 0.21273540459256773 28-29 0.224675048081267 30-31 0.23209698862829634 32-33 0.22693389955210203 34-35 0.22515908768216022 36-37 0.21701108773379113 38-39 0.2041033650433054 40-41 0.204345384843752 42-43 0.21572031546474255 44-45 0.21168665212396576 46-47 0.21983465207233488 48-49 0.22491706788171362 50-51 0.23354910743097596 52-53 0.2429072063815781 54-55 0.2249977411485292 56-57 0.2041840383101209 58-59 0.19329314729002361 60-61 0.19595536509493633 62-63 0.20200586010610147 64-65 0.1929704542227615 66-67 0.1988596027002956 68-69 0.19958566210163542 70-71 0.19942431556800433 72-73 0.20289326604107238 74-75 0.20757231551637345 76-77 0.21588166199837364 78-79 0.21765647386831544 80-81 0.2086210679849754 82-83 0.20184451357247044 84-85 0.1886947710815381 86-87 0.19369651362410129 88-89 0.20378067197604327 90-91 0.20127980070476165 92-93 0.2063622165141404 94-95 0.20257057297381026 96-97 0.21055722638854824 98-99 0.21297742439301434 100-101 0.19692344429672273 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 619784.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.944256940600074 #Duplication Level Percentage of deduplicated Percentage of total 1 73.70359642173696 17.647778501683618 2 10.713942672232625 5.130747923815947 3 4.08199893512968 2.9322129400200283 4 2.1074128866487243 2.0184174255139498 5 1.2810682477116018 1.533711364082545 6 0.9431291389707124 1.3549515859008991 7 0.7029075453895174 1.178141921060518 8 0.5417004675936518 1.037649214472448 9 0.4781274144569499 1.030356509589179 >10 4.0815786696357295 21.098611561186875 >50 0.7613675511388899 13.031578724541918 >100 0.573550091574124 25.47933395358875 >500 0.025580872628892852 3.9170333418072794 >1k 0.003365904293275375 1.7635595980705916 >5k 6.73180858655075E-4 0.8459154346654295 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5248 0.846746608495863 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4447 0.7175080350573748 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2902 0.4682276405973694 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1557 0.2512165528635783 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1030 0.16618692964000362 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1005 0.16215326629922683 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 990 0.15973306829476078 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 924 0.14908419707511003 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 905 0.14601861293611967 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 793 0.12794780116943966 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 763 0.12310740516050753 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 758 0.12230067249235219 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 741 0.11955778142062395 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 714 0.11520142501258504 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 698 0.1126198804744879 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 688 0.11100641513817718 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 677 0.10923160326823539 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 673 0.10858621713371108 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 670 0.10810217753281788 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 670 0.10810217753281788 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 657 0.10600467259561397 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 643 0.10374582112477897 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.067326681553573E-5 0.0 18-19 0.0 0.0 0.0 1.6134653363107147E-4 0.0 20-21 0.0 0.0 0.0 1.6134653363107147E-4 0.0 22-23 0.0 0.0 0.0 2.4201980044660722E-4 0.0 24-25 0.0 0.0 0.0 6.453861345242859E-4 0.0 26-27 0.0 0.0 0.0 0.0013714455358641076 0.0 28-29 0.0 0.0 0.0 0.0043563564080389295 0.0 30-31 0.0 0.0 0.0 0.009922811818310895 0.0 32-33 0.0 0.0 0.0 0.019038890968466435 0.0 34-35 0.0 0.0 0.0 0.03146257405805894 0.0 36-37 0.0 0.0 0.0 0.04445097001536019 0.0 38-39 0.0 0.0 0.0 0.056067920436797335 0.0 40-41 0.0 0.0 0.0 0.06800756392549662 0.0 42-43 0.0 0.0 0.0 0.08277077175273967 0.0 44-45 0.0 0.0 0.0 0.10213235578846824 0.0 46-47 0.0 0.0 0.0 0.1293192467053038 0.0 48-49 0.0 0.0 0.0 0.16876847417810076 0.0 50-51 0.0 0.0 0.0 0.20668490958140256 0.0 52-53 0.0 0.0 0.0 0.27235294876924865 0.0 54-55 0.0 0.0 0.0 0.38327869064061026 0.0 56-57 0.0 0.0 0.0 0.5641481548410414 0.0 58-59 0.0 0.0 0.0 0.7573606288642495 0.0 60-61 0.0 0.0 0.0 0.9458940534121565 0.0 62-63 0.0 0.0 0.0 1.1533050223948988 0.0 64-65 0.0 0.0 0.0 1.431466446374866 0.0 66-67 0.0 0.0 0.0 1.8096627212060976 0.0 68-69 0.0 0.0 0.0 2.254253094626515 0.0 70-71 0.0 0.0 0.0 2.72401352729338 0.0 72-73 0.0 0.0 0.0 3.1761871877944574 0.0 74-75 0.0 0.0 0.0 3.684993481600041 0.0 76-77 0.0 0.0 0.0 4.265921675938714 0.0 78-79 0.0 0.0 0.0 4.849431414815484 0.0 80-81 0.0 0.0 0.0 5.471664321763711 0.0 82-83 0.0 0.0 0.0 6.125440476036813 0.0 84-85 0.0 0.0 0.0 6.8456107288991 0.0 86-87 0.0 0.0 0.0 7.626931318007564 0.0 88-89 0.0 0.0 0.0 8.543782995366128 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2200 0.0 54.223476 1 GTATGGT 95 7.2759576E-12 50.02795 1 ACAATGC 70 4.3071814E-6 40.713974 8 CGAGGGG 25 0.0016165666 37.996635 86-87 GTGGTAC 115 2.4992914E-9 37.173626 6 GTATCAA 3730 0.0 35.039684 1 TGGTACT 125 5.644324E-9 34.199738 7 CATTCCG 155 3.6379788E-11 33.70942 9 GTACCAC 140 1.7007551E-8 30.540422 3 GGATATG 320 0.0 29.704096 1 GTTATAT 80 4.4765865E-4 29.704096 1 GGTACTC 150 3.331661E-8 28.499783 8 ACTCCCG 135 4.2200736E-7 28.147932 6 TCAACGC 4655 0.0 27.963493 4 ATCAACG 4660 0.0 27.933489 3 CAACGCA 4715 0.0 27.609882 5 GATTGGC 350 0.0 27.142649 9 AACGCAG 4845 0.0 26.960579 6 CGCAGAA 125 7.3748306E-6 26.604103 2 ACTGTCC 90 8.94325E-4 26.388685 8 >>END_MODULE