##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937175_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 619784 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03769216372155 31.0 31.0 34.0 30.0 34.0 2 32.26366766486389 33.0 31.0 34.0 31.0 34.0 3 32.29389432447433 34.0 31.0 34.0 30.0 34.0 4 36.00743807520039 37.0 35.0 37.0 35.0 37.0 5 35.9285573683735 37.0 35.0 37.0 35.0 37.0 6 35.96826475030011 37.0 35.0 37.0 35.0 37.0 7 35.90342764576046 37.0 35.0 37.0 35.0 37.0 8 35.88940663198792 37.0 35.0 37.0 35.0 37.0 9 37.5415112361726 39.0 37.0 39.0 35.0 39.0 10-11 37.468298633072166 39.0 37.0 39.0 34.5 39.0 12-13 37.38617728111729 39.0 37.0 39.0 34.0 39.0 14-15 38.52867289249158 40.0 38.0 41.0 34.0 41.0 16-17 38.46894401920669 40.0 38.0 41.0 34.0 41.0 18-19 38.54188717359597 40.0 38.0 41.0 34.0 41.0 20-21 38.49109125114556 40.0 38.0 41.0 34.0 41.0 22-23 38.42649858660437 40.0 38.0 41.0 34.0 41.0 24-25 38.43039187846088 40.0 38.0 41.0 34.0 41.0 26-27 38.25501223006725 40.0 38.0 41.0 34.0 41.0 28-29 38.10801989080066 40.0 37.0 41.0 33.0 41.0 30-31 38.05896005705213 40.0 37.0 41.0 34.0 41.0 32-33 37.873574503375366 40.0 37.0 41.0 33.0 41.0 34-35 37.723585152246585 40.0 36.0 41.0 33.0 41.0 36-37 37.51381852387283 40.0 36.0 41.0 33.0 41.0 38-39 37.382109735004455 40.0 36.0 41.0 33.0 41.0 40-41 37.18226269151833 40.0 36.0 41.0 33.0 41.0 42-43 36.938831108902455 39.5 36.0 41.0 31.5 41.0 44-45 36.55474729905903 39.0 35.5 41.0 31.0 41.0 46-47 36.099781536793465 39.0 35.0 41.0 30.0 41.0 48-49 35.709043795903085 39.0 35.0 40.5 28.5 41.0 50-51 35.47425474036116 39.0 35.0 40.0 27.0 41.0 52-53 35.28119393207956 39.0 35.0 40.0 27.0 41.0 54-55 35.084887638273976 39.0 35.0 40.0 26.5 41.0 56-57 34.82249864468912 38.0 35.0 40.0 25.5 41.0 58-59 34.59328169168614 38.0 34.0 40.0 25.0 41.0 60-61 34.288320608470045 38.0 34.0 40.0 23.5 41.0 62-63 33.92992962064203 38.0 34.0 40.0 22.5 41.0 64-65 33.537495320950526 37.5 33.0 40.0 22.0 41.0 66-67 33.107380151794814 37.0 33.0 39.0 20.0 40.5 68-69 32.64124194880797 36.0 32.0 39.0 18.5 40.0 70-71 32.23716488324965 36.0 31.5 39.0 16.5 40.0 72-73 31.740272417487382 35.0 31.0 38.0 12.5 40.0 74-75 31.202994914357262 35.0 31.0 37.0 8.0 39.0 76-77 29.819161998373627 33.5 29.5 36.0 7.0 38.5 78-79 30.456493713939047 34.0 30.0 36.0 4.5 39.0 80-81 30.23300940327598 34.0 30.0 36.0 2.0 38.0 82-83 29.802890200456932 34.0 30.0 35.5 2.0 37.0 84-85 29.307760445574587 34.0 29.0 35.0 2.0 37.0 86-87 28.923791191770036 34.0 29.0 35.0 2.0 36.0 88-89 28.427477960063506 34.0 29.0 35.0 2.0 36.0 90-91 27.850506789462138 33.0 27.5 35.0 2.0 35.5 92-93 27.346694170872432 33.0 26.0 35.0 2.0 35.0 94-95 26.809400533088947 33.0 25.5 35.0 2.0 35.0 96-97 26.10666215972016 32.0 25.0 34.0 2.0 35.0 98-99 25.130258283531035 32.0 20.5 34.0 2.0 35.0 100-101 22.187483058613967 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27042931996881947 1101 2 0.33711806953857604 1101 3 0.2997457780054731 1101 4 0.24046236963293666 1101 5 0.2178252864278818 1101 6 0.161980415763729 1101 7 0.2956856328445596 1101 8 0.25095333334868997 1101 9 0.40842693640618677 1101 10-11 0.31353398110263697 1101 12-13 0.32528990280678727 1101 14-15 0.3243993965099534 1101 16-17 0.3414658731013134 1101 18-19 0.40978008880718875 1101 20-21 0.4736344246922002 1101 22-23 0.3955957784629476 1101 24-25 0.44789599693453397 1101 26-27 0.32208184779884874 1101 28-29 0.3716881527974607 1101 30-31 0.4788585230249751 1101 32-33 0.49266252805860944 1101 34-35 0.5692113968970887 1101 36-37 0.6460993845349705 1101 38-39 0.3589643566752869 1101 40-41 0.5776110082609804 1101 42-43 0.7417746018486966 1101 44-45 0.5902007435641892 1101 46-47 0.6742641233738098 1101 48-49 0.4181938072688638 1101 50-51 0.7599431275619892 1101 52-53 0.6370396343437292 1101 54-55 0.5519919871208074 1101 56-57 0.6528977604705943 1101 58-59 0.9524288313012903 1101 60-61 1.0809485306199917 1101 62-63 1.1236832419608405 1101 64-65 0.8561790119009842 1101 66-67 0.9223219821845561 1101 68-69 1.1994919132650494 1101 70-71 1.1068420482873265 1101 72-73 1.0148757270503843 1101 74-75 0.6737193606198026 1101 76-77 1.0379697569865307 1101 78-79 1.1719859996801603 1101 80-81 0.9800931663533419 1101 82-83 0.9057900180420901 1101 84-85 1.2616486129037128 1101 86-87 1.2787244710818868 1101 88-89 1.5445522983700783 1101 90-91 1.608892657746157 1101 92-93 1.6766046789401052 1101 94-95 1.9648232296029597 1101 96-97 1.957051007615732 1101 98-99 2.653433867212936 1101 100-101 3.2559925461271675 1102 1 0.3201416450701302 1102 2 0.4446807654633389 1102 3 0.3479002622932583 1102 4 0.28736484688654684 1102 5 0.23555408832405078 1102 6 0.23950450017385094 1102 7 0.2552486728323302 1102 8 0.17455701099988374 1102 9 0.4208179831222978 1102 10-11 0.3645030918909242 1102 12-13 0.35404066739123863 1102 14-15 0.5153519731595395 1102 16-17 0.59093783196667 1102 18-19 0.4778758912385612 1102 20-21 0.6800841723793312 1102 22-23 0.5102547737989838 1102 24-25 0.39256158681143205 1102 26-27 0.431008849098653 1102 28-29 0.33874412037908286 1102 30-31 0.36816819884116825 1102 32-33 0.3944110371189353 1102 34-35 0.6124199755345998 1102 36-37 0.6906125353874799 1102 38-39 0.43471317156939193 1102 40-41 0.47953632747498176 1102 42-43 0.44419116657076785 1102 44-45 0.3639864878871464 1102 46-47 0.16796346965411146 1102 48-49 0.2484584490610473 1102 50-51 0.14837592058898252 1102 52-53 0.08248892328020219 1102 54-55 0.1836008639462463 1102 56-57 0.3434393729791978 1102 58-59 0.17762045527866377 1102 60-61 0.3310966688568584 1102 62-63 0.5104387278542575 1102 64-65 0.4018653220164339 1102 66-67 0.6473105134178212 1102 68-69 0.6179896003970882 1102 70-71 0.6287187208158116 1102 72-73 0.6128357201691266 1102 74-75 0.39469860215202957 1102 76-77 0.5948643972495482 1102 78-79 0.6107929612215628 1102 80-81 0.4941495735710184 1102 82-83 0.4671240267583521 1102 84-85 0.5578534068482064 1102 86-87 0.709304981824296 1102 88-89 0.7741904587798913 1102 90-91 0.9428249155640316 1102 92-93 1.145765643081095 1102 94-95 1.2884264293888776 1102 96-97 1.4960424251553022 1102 98-99 2.304286378872849 1102 100-101 3.3427274838135332 1103 1 0.29877450279992956 1103 2 0.15534309318156403 1103 3 0.2007020588793722 1103 4 0.09723626585341805 1103 5 0.08463245085346216 1103 6 0.006104713074911672 1103 7 0.10588644222686838 1103 8 -0.06568268944459277 1103 9 -0.037299803453734626 1103 10-11 -0.020647059380884514 1103 12-13 0.17899141067737645 1103 14-15 -0.040372310633827624 1103 16-17 0.006159090323976102 1103 18-19 8.608014092530425E-4 1103 20-21 0.31479194700301605 1103 22-23 0.1837738041653978 1103 24-25 0.2141040456318848 1103 26-27 0.22120909748321793 1103 28-29 0.17297543550998995 1103 30-31 0.13185386163578272 1103 32-33 -0.0026832836519972147 1103 34-35 -0.05924083946558767 1103 36-37 0.06889242557535624 1103 38-39 0.21474692759224467 1103 40-41 0.10487365425943551 1103 42-43 0.19300643755271807 1103 44-45 0.21935266836748468 1103 46-47 -0.02932601041909777 1103 48-49 -0.34245753906131426 1103 50-51 -0.4232984341714072 1103 52-53 -0.29045560105402046 1103 54-55 -0.40572186791047216 1103 56-57 -0.4232950703032188 1103 58-59 -0.35509323948846117 1103 60-61 -0.070663033081658 1103 62-63 -0.06593952635707723 1103 64-65 0.05432885804153642 1103 66-67 0.24467072706590187 1103 68-69 0.1923894155093322 1103 70-71 0.1837321671983574 1103 72-73 0.13644509341829902 1103 74-75 0.0955702825763396 1103 76-77 0.1523430137661883 1103 78-79 0.07328432378832872 1103 80-81 0.35633232298273754 1103 82-83 0.4887853086468219 1103 84-85 0.35469397383733536 1103 86-87 0.3497566495492421 1103 88-89 0.6830702070337296 1103 90-91 0.8580753544390163 1103 92-93 1.0350395851122443 1103 94-95 0.9928779757228696 1103 96-97 1.473103036592196 1103 98-99 2.010324178148238 1103 100-101 2.3340912177992656 1104 1 0.17313651695792487 1104 2 0.17917185015873116 1104 3 0.25629414842379816 1104 4 0.15988459827276813 1104 5 0.16342981767569853 1104 6 0.11566647966140664 1104 7 0.09827498385323707 1104 8 0.26118450710396957 1104 9 0.08033534259752173 1104 10-11 0.1510567468033983 1104 12-13 0.0762901962572542 1104 14-15 0.1414516632983478 1104 16-17 0.35127899639242344 1104 18-19 0.251944495594735 1104 20-21 0.17543285682828724 1104 22-23 0.1626892680547698 1104 24-25 -0.05168944605922121 1104 26-27 0.1444744472576005 1104 28-29 0.23829744912188033 1104 30-31 0.39606589934492575 1104 32-33 0.310529935027823 1104 34-35 0.2660141842107109 1104 36-37 0.5391588152410662 1104 38-39 0.6726256403114661 1104 40-41 0.7693482815937855 1104 42-43 0.8653906102702535 1104 44-45 0.5891375678711981 1104 46-47 0.9773297491452837 1104 48-49 1.1713204521052987 1104 50-51 1.2990232768025152 1104 52-53 1.1352661222074616 1104 54-55 1.1341068799648113 1104 56-57 1.221111019773673 1104 58-59 1.0712087294786343 1104 60-61 1.3744024891736046 1104 62-63 1.2557953400385102 1104 64-65 1.3847787873880861 1104 66-67 1.336366614602909 1104 68-69 1.2579537670335625 1104 70-71 1.2115794101210682 1104 72-73 1.3644020141567346 1104 74-75 1.2179395509433881 1104 76-77 1.080218472558503 1104 78-79 0.9651185601053385 1104 80-81 1.0909347636554614 1104 82-83 1.087994809203174 1104 84-85 1.0478350145023185 1104 86-87 1.2624729048482521 1104 88-89 1.3598892773208284 1104 90-91 1.3778446669502529 1104 92-93 1.5168255586393897 1104 94-95 1.7675662927600193 1104 96-97 2.0389597864425824 1104 98-99 2.3913465028173277 1104 100-101 2.7131441202793027 1105 1 0.10654965182324361 1105 2 0.17397016163418044 1105 3 0.005617403713571889 1105 4 0.03038947061585162 1105 5 0.00810787976770655 1105 6 -0.04108882214376308 1105 7 -0.09758817475558601 1105 8 0.0995251312628227 1105 9 0.22811844290892225 1105 10-11 0.0977235739484641 1105 12-13 0.015584499784644379 1105 14-15 0.00819587021044299 1105 16-17 0.01646160257624274 1105 18-19 0.03962828895399895 1105 20-21 0.3000271929619629 1105 22-23 0.1582261448970783 1105 24-25 0.025234051698816984 1105 26-27 -0.13060996802840208 1105 28-29 0.05729094318161998 1105 30-31 -0.04876600317757607 1105 32-33 0.031602791492296944 1105 34-35 0.0447586012036254 1105 36-37 0.001419766250656096 1105 38-39 -0.15749447777146486 1105 40-41 -0.3056582287231535 1105 42-43 -0.16035304299136044 1105 44-45 -0.39165645007758343 1105 46-47 -0.19011669665933795 1105 48-49 0.06778452938002744 1105 50-51 0.05852669827496726 1105 52-53 0.10985947358732062 1105 54-55 0.12078206657565715 1105 56-57 0.2197695662332606 1105 58-59 0.38396654350021464 1105 60-61 0.27416439758275146 1105 62-63 0.30259631253761654 1105 64-65 0.3229012338667161 1105 66-67 0.5045746033635794 1105 68-69 0.41847520512427394 1105 70-71 0.5218228193825638 1105 72-73 0.5119082892269269 1105 74-75 0.3567055887535595 1105 76-77 0.5957130268698663 1105 78-79 0.5650601313785373 1105 80-81 0.58270003335765 1105 82-83 0.2669125428688446 1105 84-85 0.5859592116544121 1105 86-87 1.0330931028056547 1105 88-89 1.114659315419857 1105 90-91 1.1408384936379576 1105 92-93 0.9106132938587805 1105 94-95 1.092472686438338 1105 96-97 0.9987555703832172 1105 98-99 1.1422924135723704 1105 100-101 1.3752302227768567 1106 1 -0.08931523781139461 1106 2 -0.058608708220873496 1106 3 0.009301568643785174 1106 4 0.014640577461193516 1106 5 -0.14293832276104723 1106 6 -0.0949127391223783 1106 7 -0.12779331161227958 1106 8 -0.13595137078672082 1106 9 -0.12020840817876888 1106 10-11 -0.31998116394235154 1106 12-13 -0.5200933090491517 1106 14-15 -0.33081911722783275 1106 16-17 -0.08868300545687191 1106 18-19 0.04216457205107105 1106 20-21 0.09966196819598849 1106 22-23 0.12768473679830805 1106 24-25 -0.08000174853553688 1106 26-27 -0.04473541202043663 1106 28-29 -0.012375338957930637 1106 30-31 -0.0808166422069263 1106 32-33 -0.057098722754211906 1106 34-35 -0.39916161473951206 1106 36-37 0.18244312513300542 1106 38-39 0.18529130718820852 1106 40-41 0.005311737125367699 1106 42-43 0.14427564516322633 1106 44-45 -0.0872306645707539 1106 46-47 0.12343286858184399 1106 48-49 0.2852585510016965 1106 50-51 0.10759494195876584 1106 52-53 0.11379555697346433 1106 54-55 0.2133854671328379 1106 56-57 -0.08446357574843688 1106 58-59 0.21032405340597649 1106 60-61 0.19642646573068134 1106 62-63 0.2832715451917167 1106 64-65 0.18673987838584338 1106 66-67 0.047342605844015395 1106 68-69 0.15892396194659142 1106 70-71 0.09323771198094732 1106 72-73 0.20889826264440003 1106 74-75 0.1339617978025629 1106 76-77 -0.13432272047302263 1106 78-79 -0.11749981609475313 1106 80-81 0.12833603056716925 1106 82-83 0.23206116335882143 1106 84-85 0.26960206651746077 1106 86-87 0.1655224174506671 1106 88-89 0.3711898169313699 1106 90-91 0.2860062188940944 1106 92-93 0.2169518357866771 1106 94-95 -0.10782300427419145 1106 96-97 0.4319071272620718 1106 98-99 0.31353654877392856 1106 100-101 0.96584677324946 1107 1 -0.16989327567967294 1107 2 0.016824682377283295 1107 3 -0.08722946919693442 1107 4 -0.11482538977843149 1107 5 0.07281072869461269 1107 6 -0.06545044663080546 1107 7 0.024093212167933586 1107 8 -0.08470241579319548 1107 9 -0.1535062189476193 1107 10-11 -0.10431249760028294 1107 12-13 0.05039524870411327 1107 14-15 -0.1814911367774883 1107 16-17 -0.05273707937443106 1107 18-19 -0.11048446194953243 1107 20-21 -0.15119930087825395 1107 22-23 -0.07408878847937217 1107 24-25 -0.2814789972516749 1107 26-27 -0.14392375204486996 1107 28-29 -0.06929488953624485 1107 30-31 -0.02221819111323242 1107 32-33 0.05882579792083931 1107 34-35 -0.06700573192722459 1107 36-37 0.09773329242590734 1107 38-39 0.16294424560861387 1107 40-41 0.21607691153809583 1107 42-43 0.19514334504655295 1107 44-45 0.19955815684567568 1107 46-47 0.42090142651883866 1107 48-49 0.019539816217445605 1107 50-51 -0.014353868800895953 1107 52-53 -0.17167035514493278 1107 54-55 -0.0827693683762476 1107 56-57 -0.09785803239099522 1107 58-59 0.015413120111837486 1107 60-61 0.030049951045469925 1107 62-63 0.009325968210340818 1107 64-65 0.21959212595361777 1107 66-67 0.4433679064843403 1107 68-69 0.2730363724894431 1107 70-71 0.029248864841285638 1107 72-73 0.060162835317132846 1107 74-75 0.30417153404752284 1107 76-77 -0.01448459312462802 1107 78-79 0.22052418001001328 1107 80-81 -0.03705519229711385 1107 82-83 0.2113838154244867 1107 84-85 0.20693153524076635 1107 86-87 0.5412194712214173 1107 88-89 0.5583286580995726 1107 90-91 0.5104047085726435 1107 92-93 1.0134580690780552 1107 94-95 0.7453386570794045 1107 96-97 1.2310224316690181 1107 98-99 1.1184464715123497 1107 100-101 1.405541861030006 1108 1 -0.04883008441989389 1108 2 0.03701031091788565 1108 3 -2.1503704154923753E-4 1108 4 0.01245492449186969 1108 5 -0.03883615557838738 1108 6 -0.0011759321986559712 1108 7 -0.018136859318445886 1108 8 0.05101211077815293 1108 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44-45 -0.10648417577871783 2316 46-47 0.018674633686352138 2316 48-49 -0.0065105397382794195 2316 50-51 -0.16073509165847355 2316 52-53 0.26651815493517717 2316 54-55 -0.1284673345254319 2316 56-57 0.11901707108450665 2316 58-59 -0.6176500094968063 2316 60-61 -0.19131121348376467 2316 62-63 -0.4310848772959588 2316 64-65 -0.11626707094912803 2316 66-67 0.05073090491787724 2316 68-69 0.14251396447121323 2316 70-71 0.13195068983077363 2316 72-73 0.21492079815510223 2316 74-75 0.2610429442435205 2316 76-77 0.11086722358682621 2316 78-79 0.36816302821622315 2316 80-81 0.3053181855253797 2316 82-83 0.3208545302651018 2316 84-85 -0.006517733107830992 2316 86-87 -0.22607526521851895 2316 88-89 0.4417229093189903 2316 90-91 0.6052869693691179 2316 92-93 0.35121484751948984 2316 94-95 0.40416559509598216 2316 96-97 0.4677234415899285 2316 98-99 0.6455666049798303 2316 100-101 1.26368490556586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 0.0 5 4.0 6 34.0 7 76.0 8 168.0 9 379.0 10 625.0 11 1127.0 12 1724.0 13 2399.0 14 3055.0 15 4670.0 16 6705.0 17 9386.0 18 7705.0 19 4098.0 20 3324.0 21 3347.0 22 3698.0 23 3921.0 24 4734.0 25 5364.0 26 6448.0 27 7899.0 28 9637.0 29 12238.0 30 15267.0 31 18908.0 32 23663.0 33 31089.0 34 41734.0 35 58793.0 36 86590.0 37 134171.0 38 99715.0 39 7086.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.009841506071645 25.666363743484865 17.478721395146877 19.84507335529661 2 14.58846953132059 24.17455113394344 40.746453603190794 20.490525731545183 3 15.850844810450093 29.610961238108764 33.81758806293805 20.72060588850309 4 11.083538781252823 21.52798394279297 42.04690666425723 25.34157061169698 5 10.175578997641091 40.55484383117826 37.612661307758586 11.656915863422064 6 24.90206265408594 43.65230467388639 18.71910213880965 12.726530533218025 7 23.148225833516193 36.67648729234701 24.99257805945297 15.182708814683826 8 23.296180604855884 37.15697726950034 22.04558362268145 17.501258502962322 9 24.731680714571528 17.83669794638132 24.752010377809043 32.67961096123811 10-11 21.133491668065002 29.813531811082573 30.77241426045203 18.280562260400398 12-13 22.361545319225325 29.029798666381602 30.255307647934426 18.35334836645865 14-15 20.677929730357672 30.489654460263576 28.751145560388778 20.08127024898997 16-17 17.30578717746828 33.0119041472513 30.717556439017464 18.964752236262957 18-19 17.08095078285338 31.087927406967587 34.58003110761169 17.251090702567346 20-21 17.59929072006596 30.422720191808917 34.93784523143969 17.04014385668544 22-23 18.39220757637974 29.870284931060382 34.312818307742326 17.424689184817552 24-25 18.01651865811315 30.75789307242523 34.31695558452622 16.908632684935395 26-27 17.530688506815366 30.757644697439073 34.731177070425446 16.980489725320115 28-29 17.139342013972925 29.735063007244623 35.624425189989836 17.501169788792616 30-31 18.093414877937214 29.752244130410055 34.943653711336296 17.210687280316435 32-33 17.735144610981095 30.717742566557515 34.40725672755083 17.13985609491056 34-35 18.13397893459657 30.049420443251197 34.81729118531617 16.99930943683606 36-37 18.671827604455746 29.670336762484993 33.844290914253996 17.81354471880526 38-39 17.484397790197875 31.138267525460485 33.876398874446586 17.50093580989506 40-41 17.616701947775354 30.319837233616877 34.91345371936029 17.150007099247482 42-43 17.78530068827639 30.253986976723173 34.116434968732676 17.84427736626776 44-45 17.8786480451254 30.676493746208354 33.62679578691931 17.81806242174693 46-47 18.506286060950266 30.886728279529642 32.74414957469054 17.862836084829553 48-49 18.045157667832665 30.976759645295783 32.75810604984962 18.219976637021933 50-51 18.114859370361287 30.82856285415564 32.32779484465556 18.728782930827514 52-53 18.22128210899451 31.604019790781777 32.36154237729788 17.813155722925828 54-55 18.85681140526377 31.2287022575607 31.701124908032476 18.213361429143056 56-57 18.528148516257275 31.65094613607321 31.615369225407562 18.20553612226195 58-59 18.140352122674997 30.67713913234288 32.411291675809636 18.771217069172486 60-61 18.74419152478928 31.569708156389968 31.5080737805429 18.17802653827785 62-63 18.442150813831915 30.976114259161257 32.52318549688278 18.058549430124042 64-65 18.53565113007112 30.713684122210317 32.103523162908374 18.647141584810193 66-67 18.2686226169117 31.231041782298348 31.40755489009074 19.092780710699213 68-69 18.215136241012996 30.399945787564697 32.1987176177507 19.1862003536716 70-71 18.416335368450945 30.782982458404863 32.44549714093942 18.35518503220477 72-73 18.76850446043212 30.097792291818266 32.184329031289984 18.94937421645963 74-75 18.150839647361018 31.15795180256347 31.403521226749966 19.287687323325546 76-77 18.049352677707073 30.83033766602558 32.28269848850567 18.837611167761672 78-79 18.123249390110104 30.684077033289014 31.64408890839389 19.54858466820699 80-81 18.06927897461051 30.911575645708826 31.229831683296116 19.789313696384546 82-83 18.18529448305127 30.854688994983714 31.40399592759378 19.556020594371237 84-85 17.700521471996698 30.49659236120971 31.269200237502098 20.533685929291494 86-87 17.37258464239154 30.90423437842861 31.712499838653468 20.010681140526376 88-89 18.277012636660515 30.04353129477366 31.566481225717347 20.112974842848477 90-91 17.82043938134848 29.902942386665266 31.890243410489965 20.386374821496283 92-93 18.091066845978908 30.163986798445563 31.610720333411052 20.134226022164476 94-95 17.903818104371847 29.379428962348175 31.714839363391118 21.001913569888863 96-97 18.61253275334633 29.20961173570147 30.907138616033976 21.27071689491823 98-99 17.935133800754617 29.64260907236156 30.93265632273205 21.489600804151774 100-101 17.95543313477608 29.512820699008667 31.418357146487814 21.113389019727443 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1158.0 1 754.0 2 375.5 3 443.5 4 404.0 5 391.0 6 656.0 7 1291.0 8 1970.5 9 2172.0 10 1854.5 11 1467.5 12 1343.5 13 1287.5 14 1225.5 15 1210.5 16 1238.0 17 1331.0 18 1398.0 19 1525.0 20 1753.5 21 1995.0 22 2276.5 23 2599.5 24 2979.5 25 3814.0 26 5977.5 27 8376.0 28 8778.0 29 9740.0 30 12803.0 31 15594.5 32 19674.5 33 25450.5 34 30587.5 35 34366.0 36 39459.0 37 43131.0 38 40899.0 39 36906.0 40 33301.5 41 29442.5 42 25569.5 43 23587.5 44 22636.0 45 21528.5 46 18824.5 47 14615.0 48 12010.5 49 9967.5 50 7905.0 51 5816.5 52 4243.0 53 3568.5 54 3114.5 55 2607.5 56 2146.0 57 1861.5 58 1593.0 59 1264.5 60 962.0 61 796.5 62 625.0 63 428.5 64 280.5 65 186.0 66 133.0 67 113.5 68 90.0 69 76.0 70 76.5 71 70.5 72 57.5 73 51.0 74 44.0 75 34.0 76 26.0 77 16.0 78 12.5 79 10.0 80 8.0 81 5.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04275683141223394 2 0.0 3 0.0 4 0.0 5 9.680792017864288E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006695881145689466 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.067326681553573E-5 22-23 4.0336633407767866E-4 24-25 0.0 26-27 0.0012907722690485718 28-29 0.0022588514708350004 30-31 8.067326681553573E-5 32-33 8.067326681553573E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0076639603474758945 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.067326681553573E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.6134653363107147E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.840396008932144E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007905980147922502 92-93 0.0013714455358641074 94-95 0.0 96-97 0.0 98-99 8.067326681553573E-5 100-101 0.010245504885573038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 619784.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.310136306630692 #Duplication Level Percentage of deduplicated Percentage of total 1 72.64409054172208 16.933436523987634 2 11.266384631611128 5.2524192289156915 3 4.158341235365123 2.907945030175323 4 2.172111371571565 2.025288485780629 5 1.3361864587102033 1.5573344241804503 6 0.9966696068522407 1.393950263304105 7 0.7202812383305396 1.1752897691215538 8 0.5771190710972494 1.0762179369946376 9 0.46918542984524464 0.9843098690499987 >10 4.2301419617589024 20.969226685485523 >50 0.8193324677914421 13.477822086003114 >100 0.5788837537491698 25.28049413545188 >500 0.02640766223585649 3.8796870104899526 >1k 0.004169630879345761 2.1974093652315743 >5k 6.949384798909602E-4 0.8891691858279477 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5489 0.8856311231009513 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4830 0.7793037574380752 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3457 0.5577749667626141 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1947 0.3141417009796961 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1218 0.19652007796264503 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1089 0.17570637512423684 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1024 0.16521885043821719 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 974 0.1571515237566636 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 839 0.13536974171646896 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 799 0.12891588037122612 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 793 0.12794780116943966 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 766 0.12359144476140076 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 758 0.12230067249235219 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 748 0.12068720715604146 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 709 0.11439469234442969 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 708 0.1142333458107986 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 692 0.11165180127270145 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 671 0.10826352406644896 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 655 0.10568197952835182 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 652 0.1051979399274586 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 636 0.10261639538936146 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 630 0.10164831618757503 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 626 0.10100293005305074 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 625 0.10084158351941967 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.067326681553573E-5 0.0 18-19 0.0 0.0 0.0 1.6134653363107147E-4 0.0 20-21 0.0 0.0 0.0 1.6134653363107147E-4 0.0 22-23 0.0 0.0 0.0 2.4201980044660722E-4 0.0 24-25 0.0 0.0 0.0 4.8403960089321444E-4 0.0 26-27 0.0 0.0 0.0 0.0012100990022330359 0.0 28-29 0.0 0.0 0.0 0.0043563564080389295 0.0 30-31 0.0 0.0 0.0 0.010003485085126431 0.0 32-33 0.0 0.0 0.0 0.018958217701650897 0.0 34-35 0.0 0.0 0.0 0.0313819007912434 0.0 36-37 0.0 0.0 0.0 0.04437029674854465 0.0 38-39 0.0 0.0 0.0 0.055906573903166265 0.0 40-41 0.0 0.0 0.0 0.06784621739186555 0.0 42-43 0.0 0.0 0.0 0.08244807868547752 0.0 44-45 0.0 0.0 0.0 0.10140629638712842 0.0 46-47 0.0 0.0 0.0 0.1289158803712261 0.0 48-49 0.0 0.0 0.0 0.16852645437765415 0.0 50-51 0.0 0.0 0.0 0.2061201967136938 0.0 52-53 0.0 0.0 0.0 0.27219160223561756 0.0 54-55 0.0 0.0 0.0 0.3831980173737948 0.0 56-57 0.0 0.0 0.0 0.5639868083074103 0.0 58-59 0.0 0.0 0.0 0.7571186090638029 0.0 60-61 0.0 0.0 0.0 0.9458940534121565 0.0 62-63 0.0 0.0 0.0 1.1537890619957922 0.0 64-65 0.0 0.0 0.0 1.4312244265744194 0.0 66-67 0.0 0.0 0.0 1.8114375330760395 0.0 68-69 0.0 0.0 0.0 2.2603842629044957 0.0 70-71 0.0 0.0 0.0 2.73304893317672 0.0 72-73 0.0 0.0 0.0 3.1887722174176814 0.0 74-75 0.0 0.0 0.0 3.700321402294993 0.0 76-77 0.0 0.0 0.0 4.2871387451112 0.0 78-79 0.0 0.0 0.0 4.874924167129193 0.0 80-81 0.0 0.0 0.0 5.502723529487692 0.0 82-83 0.0 0.0 0.0 6.16343758470693 0.0 84-85 0.0 0.0 0.0 6.892320550385296 0.0 86-87 0.0 0.0 0.0 7.6832412582448075 0.0 88-89 0.0 0.0 0.0 8.614291430562906 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2220 0.0 57.129494 1 GTATCAA 3965 0.0 39.29459 1 GTGTAGC 105 4.650792E-8 36.19118 1 TTCCGCT 30 0.003952253 31.664732 80-81 TCAACGC 5135 0.0 29.783962 4 ATCAACG 5180 0.0 29.525223 3 CAACGCA 5215 0.0 29.327065 5 AACGCAG 5330 0.0 28.605192 6 GACATAT 250 0.0 28.500557 1 TATCAAC 5375 0.0 28.454073 2 TCGAGTA 35 0.008387719 27.145575 10-11 GGATATG 420 0.0 27.143387 1 CTCCGAC 55 9.4629686E-5 25.914825 94-95 GACTGGT 110 9.463268E-5 25.911688 7 CATATAG 275 0.0 25.907507 3 ACGCAGA 5915 0.0 25.53933 7 GAGTACC 205 9.549694E-10 25.488304 1 GATTGGC 450 0.0 25.335875 9 CGCAGAG 5870 0.0 25.249548 8 TATAGAC 95 0.0012263544 24.99847 3 >>END_MODULE