FastQCFastQC Report
Thu 26 May 2016
SRR937174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607874
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54130.8904805930176319No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44210.7272888789453078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28820.47411141124640965No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15170.24955829662068127No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9940.16352072962488937No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA9570.15743394190243373No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8600.14147668760302298No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT8430.13868005540621905No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.13785751652480613No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC8120.13358031434145892No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8080.1329222832363286No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8020.13193523657863307No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG6960.11449741229267907No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG6840.11252331897728805No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG6830.11235881120100547No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC6830.11235881120100547No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA6820.11219430342472288No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6510.10709456235996274No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA6300.10363989905802846No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA6290.10347539128174588No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT6140.10100777463750713No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC200.002064395271.329971
GGTATCA20600.059.3262251
GTATCAA38100.039.0661131
GTATGGT1002.996967E-838.0426521
TACAGGG550.0025728434.5613942
CATTCCG2450.032.958079
TCAACGC45000.032.2119564
ATCAACG45250.032.031353
GATCCTC902.402148E-531.6838915
ATACTCG1201.5042679E-731.6655966
CGCAGAA600.00395014631.6655969
TACTCGC1201.5042679E-731.6655967
CAACGCA45950.031.5459885
TAGGCAT2500.030.4165365
AACGCAG47650.030.4029566
ACTCGCC1252.1517008E-730.398978
TATCAAC48500.030.0808812
ATGGGTA1951.6370905E-1129.2466685
GGCATTC2850.028.3323757
GCCTATG856.4049265E-427.940239
Produced by FastQC (version 0.11.5)