##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937174_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 607874 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.925731319319464 33.0 31.0 34.0 30.0 34.0 2 32.087865248390294 33.0 31.0 34.0 30.0 34.0 3 32.074536828355875 34.0 31.0 34.0 30.0 34.0 4 35.7289356017859 37.0 35.0 37.0 35.0 37.0 5 35.748133659278075 37.0 35.0 37.0 35.0 37.0 6 35.8084142437413 37.0 35.0 37.0 35.0 37.0 7 35.772635447477604 37.0 35.0 37.0 35.0 37.0 8 35.777147895781034 37.0 35.0 37.0 35.0 37.0 9 37.4273007235052 39.0 37.0 39.0 35.0 39.0 10-11 37.335763661548285 39.0 37.0 39.0 35.0 39.0 12-13 37.26185443035892 39.0 37.0 39.0 34.0 39.0 14-15 38.457862155644094 40.0 38.0 41.0 34.0 41.0 16-17 38.410590846129296 40.0 38.0 41.0 34.0 41.0 18-19 38.4654780431471 40.0 38.0 41.0 34.0 41.0 20-21 38.46480438380322 40.0 38.0 41.0 34.0 41.0 22-23 38.32903447095944 40.0 38.0 41.0 34.0 41.0 24-25 38.19447698042686 40.0 38.0 41.0 34.0 41.0 26-27 38.035376574750686 40.0 38.0 41.0 34.0 41.0 28-29 38.088062657721835 40.0 38.0 41.0 34.0 41.0 30-31 37.90294205707104 40.0 37.5 41.0 34.0 41.0 32-33 37.67219686974603 40.0 37.0 41.0 33.0 41.0 34-35 37.55629949627719 40.0 37.0 41.0 33.0 41.0 36-37 37.49513468251644 40.0 37.0 41.0 33.0 41.0 38-39 37.401438455995816 40.0 37.0 41.0 33.0 41.0 40-41 37.26397575813409 40.0 37.0 41.0 33.0 41.0 42-43 37.00894017510208 40.0 36.0 41.0 32.0 41.0 44-45 36.72162816636342 40.0 36.0 41.0 31.0 41.0 46-47 36.29711667220509 40.0 36.0 41.0 30.0 41.0 48-49 35.93886068494457 40.0 36.0 41.0 29.5 41.0 50-51 35.06296206121664 38.5 35.0 40.0 27.0 40.5 52-53 35.17206197336948 38.5 35.0 40.0 27.5 40.5 54-55 35.54769902973314 39.0 36.0 40.5 27.5 41.0 56-57 35.4170469538095 39.0 35.0 41.0 27.0 41.0 58-59 35.25576928771423 39.0 35.0 41.0 26.5 41.0 60-61 35.021927241500705 39.0 35.0 40.5 26.0 41.0 62-63 34.738917111111846 38.5 35.0 40.0 25.5 41.0 64-65 34.431917634246574 38.0 35.0 40.0 23.5 41.0 66-67 34.08660347374621 38.0 34.0 40.0 22.5 41.0 68-69 33.6318126782853 37.0 34.0 39.5 21.5 41.0 70-71 33.24517992215492 37.0 34.0 39.0 20.0 41.0 72-73 32.85156956869351 36.0 33.0 39.0 18.5 40.0 74-75 32.36758275563686 36.0 33.0 38.5 15.0 40.0 76-77 31.93856621602503 35.0 32.5 37.5 10.5 39.5 78-79 31.464219558658538 35.0 32.0 37.0 7.0 39.0 80-81 30.963678328074565 35.0 31.5 36.5 2.0 39.0 82-83 30.47544474677318 35.0 31.0 36.0 2.0 37.5 84-85 30.029210823295617 34.5 31.0 35.5 2.0 37.0 86-87 29.621305566614133 34.0 30.5 35.0 2.0 36.5 88-89 29.229896327199388 34.0 30.0 35.0 2.0 36.0 90-91 28.83954816294166 34.0 29.0 35.0 2.0 36.0 92-93 28.445142414381927 34.0 29.0 35.0 2.0 35.5 94-95 27.956258204825343 34.0 29.0 35.0 2.0 35.0 96-97 27.41074959613341 34.0 27.5 35.0 2.0 35.0 98-99 26.77684191131715 33.5 26.0 35.0 2.0 35.0 100-101 24.87891158365056 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04355073011197064 1101 2 5.687264403491099E-4 1101 3 0.1395320251461527 1101 4 0.07656723330541126 1101 5 0.06384092482115733 1101 6 -0.013809015167140615 1101 7 0.04444294022228945 1101 8 0.11844788153053543 1101 9 0.010464265756141344 1101 10-11 0.014350409145968968 1101 12-13 -0.11546002135485622 1101 14-15 0.007410945659913182 1101 16-17 0.08183510948413186 1101 18-19 0.06902830504207458 1101 20-21 0.17025512541026444 1101 22-23 0.3134037128199836 1101 24-25 0.20803895917320858 1101 26-27 -0.0016725622372533167 1101 28-29 0.029722856445047796 1101 30-31 -0.011236132486288852 1101 32-33 0.01630147567129825 1101 34-35 -0.09666902913782849 1101 36-37 0.19012113228797034 1101 38-39 0.16325017626835603 1101 40-41 0.20632158888249563 1101 42-43 0.2571967683586962 1101 44-45 0.3640504869100951 1101 46-47 0.13188812228762714 1101 48-49 0.21436009624901686 1101 50-51 0.29762882104503063 1101 52-53 0.2876286237224619 1101 54-55 0.3568810985858448 1101 56-57 0.42196396178938755 1101 58-59 0.5426743536096481 1101 60-61 0.45950974870478944 1101 62-63 0.3568125810086755 1101 64-65 0.5226787270482518 1101 66-67 0.3366301794261375 1101 68-69 0.5100018048814619 1101 70-71 0.7885327776384585 1101 72-73 0.5814013870377366 1101 74-75 0.29531858908845976 1101 76-77 0.46216093363634414 1101 78-79 0.548224262979609 1101 80-81 0.5275331973300545 1101 82-83 0.5578611521682895 1101 84-85 0.3323344080193458 1101 86-87 0.6090771629981866 1101 88-89 0.8885908123360586 1101 90-91 1.0811060514306057 1101 92-93 0.7467884534805904 1101 94-95 1.0853175009405298 1101 96-97 1.0520087609984827 1101 98-99 1.2212609518661033 1101 100-101 2.113866399549103 1102 1 0.015747572510555585 1102 2 -0.09306419656599729 1102 3 0.032270384347931724 1102 4 -0.24085000042805405 1102 5 -0.079466094064081 1102 6 -0.25908292754576223 1102 7 0.007546961936483854 1102 8 -0.08379426236425758 1102 9 -0.10440976895599619 1102 10-11 0.09695420148483436 1102 12-13 0.07470646690877203 1102 14-15 0.1520177581073483 1102 16-17 0.1465380684259543 1102 18-19 0.05160533692525604 1102 20-21 -0.07884887718932276 1102 22-23 -0.12344400107251374 1102 24-25 -0.023565616864715366 1102 26-27 0.08754010692705805 1102 28-29 0.09487023009746309 1102 30-31 0.1784238185803062 1102 32-33 0.20017686752082398 1102 34-35 0.13475395809361146 1102 36-37 0.05389129820279237 1102 38-39 0.002097278493295107 1102 40-41 0.1312107241374818 1102 42-43 0.31679851543416504 1102 44-45 0.2664106635291077 1102 46-47 0.47688057201619216 1102 48-49 0.42137611429321 1102 50-51 0.22803968961295595 1102 52-53 0.1961222012130932 1102 54-55 0.2531126529860259 1102 56-57 0.18525339523230855 1102 58-59 0.4558534001728347 1102 60-61 0.38212185589951986 1102 62-63 0.24427052886401412 1102 64-65 0.49880696697484694 1102 66-67 0.40790617529737716 1102 68-69 0.45015156786028143 1102 70-71 0.4670943075719478 1102 72-73 0.3702781169100504 1102 74-75 0.36479064352687374 1102 76-77 0.5343162016231879 1102 78-79 0.7675477013158769 1102 80-81 0.6412818593072664 1102 82-83 1.006476298586211 1102 84-85 0.9174230998153554 1102 86-87 0.790759153211507 1102 88-89 0.7872021358317376 1102 90-91 1.0208472395948398 1102 92-93 0.9840796263531324 1102 94-95 0.7897291532075208 1102 96-97 0.8745295959247024 1102 98-99 1.0027522738326162 1102 100-101 1.2780585205063595 1103 1 -0.06512689038029862 1103 2 -0.1667131649884297 1103 3 -0.28584348981975793 1103 4 -0.3860088722762711 1103 5 -0.31960132608117675 1103 6 -0.07432206704653765 1103 7 -0.016010867791131034 1103 8 -0.21119866045058444 1103 9 -0.2923288546638503 1103 10-11 -0.16573727177568287 1103 12-13 0.029470982327374884 1103 14-15 -0.06428552522856279 1103 16-17 -0.10178441729409116 1103 18-19 0.04693945747198569 1103 20-21 -0.10253439480556636 1103 22-23 -0.1793537245638035 1103 24-25 -0.14975639428480747 1103 26-27 0.016353523292664818 1103 28-29 -0.10873689418384913 1103 30-31 -0.13260630638981752 1103 32-33 -0.057736267228548854 1103 34-35 0.1340300029085455 1103 36-37 0.022697967024924992 1103 38-39 -0.08977240020180233 1103 40-41 -0.06664224501593452 1103 42-43 -0.08891159322940467 1103 44-45 0.09850694722582176 1103 46-47 -0.15422443194329816 1103 48-49 -0.23170292099550238 1103 50-51 -0.05508067437953201 1103 52-53 -0.3781698924465573 1103 54-55 -0.2377746489806043 1103 56-57 -0.12717426782160857 1103 58-59 -0.41712526540672457 1103 60-61 -0.37275605573577053 1103 62-63 -0.4821739843054118 1103 64-65 -0.23371619256238318 1103 66-67 -0.2940925501666314 1103 68-69 -0.08824617171265459 1103 70-71 -0.3816623169694324 1103 72-73 -0.13786813609471693 1103 74-75 -0.1368107265993146 1103 76-77 0.05388524914182824 1103 78-79 -0.16179638122907392 1103 80-81 -0.11932209269145844 1103 82-83 -0.26682029812154795 1103 84-85 -0.4452960196302236 1103 86-87 -0.329668097492533 1103 88-89 -0.2960199098922409 1103 90-91 -0.20109071111997778 1103 92-93 -0.30275422944624 1103 94-95 0.10176906680148079 1103 96-97 0.360222695155759 1103 98-99 0.39555184836251556 1103 100-101 0.46170650491204057 1104 1 0.07687609915632265 1104 2 0.0851014298754933 1104 3 0.15607600815355482 1104 4 0.09301050584624448 1104 5 0.06820351912277545 1104 6 0.0416504076703319 1104 7 0.20054874114516252 1104 8 0.10512663345318884 1104 9 0.1857142108231571 1104 10-11 0.1668352110210165 1104 12-13 0.15996021595562837 1104 14-15 0.1904613741371648 1104 16-17 0.23817437557950427 1104 18-19 0.1805697245103275 1104 20-21 0.20080930764127203 1104 22-23 0.38881983015683375 1104 24-25 0.2392065597005626 1104 26-27 0.20935706495090756 1104 28-29 0.23262240233240306 1104 30-31 0.17177767400312405 1104 32-33 0.20689832251814266 1104 34-35 0.37935043544870695 1104 36-37 0.38057378005769493 1104 38-39 0.3421920754846397 1104 40-41 0.3813472242739877 1104 42-43 0.4824837931884005 1104 44-45 0.46523017269343825 1104 46-47 0.6650836653916983 1104 48-49 0.5419575107366725 1104 50-51 0.37303578288491934 1104 52-53 0.5655574672000867 1104 54-55 0.5243854932243863 1104 56-57 0.6949213704175321 1104 58-59 0.6402650845845201 1104 60-61 0.7437719072047457 1104 62-63 0.393100393211121 1104 64-65 0.4829141148751006 1104 66-67 0.8515833948366733 1104 68-69 1.0022310768363454 1104 70-71 0.9264855169631545 1104 72-73 1.0165003579598704 1104 74-75 0.5198571520416593 1104 76-77 0.507510398955322 1104 78-79 0.5859883990669168 1104 80-81 0.5355648570385405 1104 82-83 0.6997393108147456 1104 84-85 0.6700166024088539 1104 86-87 0.7175080023741494 1104 88-89 0.6654393071723597 1104 90-91 0.4466758895614227 1104 92-93 0.48027880358679553 1104 94-95 0.5930630513690112 1104 96-97 0.8036201284635105 1104 98-99 0.6568703417437867 1104 100-101 1.0366603825565015 1105 1 0.2357966388864554 1105 2 0.22280059830929133 1105 3 0.34615889463823635 1105 4 0.3376562010166779 1105 5 0.1209564219506376 1105 6 0.04185751132570914 1105 7 0.14363800732288468 1105 8 0.14279698746536695 1105 9 0.22528901801415913 1105 10-11 0.10462082271967432 1105 12-13 0.18779764859065295 1105 14-15 0.36940906245516913 1105 16-17 0.14500474417898346 1105 18-19 0.15488324342654636 1105 20-21 0.2470677099716383 1105 22-23 0.30305865668951526 1105 24-25 0.1838761168138987 1105 26-27 0.27126642558590675 1105 28-29 0.36936934417679623 1105 30-31 0.539027607594889 1105 32-33 0.4747441293444581 1105 34-35 0.5287160679477765 1105 36-37 0.41084918289681127 1105 38-39 0.5480970059023136 1105 40-41 0.4629539434369292 1105 42-43 0.5425719923320784 1105 44-45 0.6749563906655425 1105 46-47 0.7095028972172273 1105 48-49 0.7258261619966291 1105 50-51 0.5907219848289884 1105 52-53 0.6275996443097256 1105 54-55 0.6578761803132664 1105 56-57 0.5644955923155948 1105 58-59 0.7057852524285337 1105 60-61 0.6805749021036931 1105 62-63 0.5272540371098913 1105 64-65 0.38425060879031037 1105 66-67 0.5520045806401654 1105 68-69 0.6693828613044772 1105 70-71 0.7622421881770975 1105 72-73 0.653295699028078 1105 74-75 0.533694827798378 1105 76-77 0.5317606612115817 1105 78-79 0.7495226229459675 1105 80-81 0.9233112991328092 1105 82-83 1.0130184820399428 1105 84-85 0.8521034868945385 1105 86-87 1.0178274372513059 1105 88-89 0.9072103135610554 1105 90-91 1.0821802094735382 1105 92-93 1.12003437625091 1105 94-95 1.2364035432852134 1105 96-97 1.0779165388168899 1105 98-99 1.2141806505533488 1105 100-101 1.3819156828342862 1106 1 0.06197296949909514 1106 2 0.05414275137582791 1106 3 -0.024361508349535654 1106 4 -0.01445090489385592 1106 5 0.011468691896141081 1106 6 0.04566037889341601 1106 7 0.04287765724456705 1106 8 0.11053740935576428 1106 9 0.05304950438795686 1106 10-11 -0.35922288880757236 1106 12-13 -0.2896108155826127 1106 14-15 -0.07363618094399271 1106 16-17 0.03963380818647266 1106 18-19 0.11027998618779833 1106 20-21 0.08795054724152607 1106 22-23 -0.09480686658461224 1106 24-25 -0.05889631217279856 1106 26-27 -0.11706958524622735 1106 28-29 0.16414408795244384 1106 30-31 0.08799008611718051 1106 32-33 0.13575937433001428 1106 34-35 0.08968483840606467 1106 36-37 0.09811248120294636 1106 38-39 0.06230078329826938 1106 40-41 -0.05954933547503316 1106 42-43 -0.2531434137502728 1106 44-45 -0.011763960038393861 1106 46-47 0.278553319844967 1106 48-49 0.3544790486680469 1106 50-51 0.3901497672120158 1106 52-53 0.2822648601369835 1106 54-55 0.39948451176601907 1106 56-57 0.44980964169924675 1106 58-59 0.3735136088166584 1106 60-61 0.3380163006294623 1106 62-63 0.38475822093452194 1106 64-65 0.578975526510277 1106 66-67 0.7404129844859355 1106 68-69 0.4425458327248748 1106 70-71 0.302414724859581 1106 72-73 0.37598241826160717 1106 74-75 0.2749222021886695 1106 76-77 0.3038958305866508 1106 78-79 0.4516912852616066 1106 80-81 0.5953776504578911 1106 82-83 0.6547110485733647 1106 84-85 0.3552025334964277 1106 86-87 0.4053847763164953 1106 88-89 0.3864621183591055 1106 90-91 0.5948232918071845 1106 92-93 0.5675885425358764 1106 94-95 0.5916331564964885 1106 96-97 0.4746760577161595 1106 98-99 0.6958120405215524 1106 100-101 0.5380410478715909 1107 1 -0.01188129729338172 1107 2 -0.016298786909338503 1107 3 0.019763660302743347 1107 4 0.0764229123355733 1107 5 -0.014841063921807063 1107 6 0.008190912672375816 1107 7 0.007361471034812439 1107 8 0.028590037685823688 1107 9 -0.09392489573902196 1107 10-11 0.012293835325792202 1107 12-13 0.0500129906237845 1107 14-15 0.13658723372456194 1107 16-17 0.05926691782588733 1107 18-19 -0.11799363174533539 1107 20-21 -0.04394876403987524 1107 22-23 0.19129464224702986 1107 24-25 -0.13143553209537373 1107 26-27 -0.008194066061754768 1107 28-29 0.15289178414024462 1107 30-31 0.15780882349581304 1107 32-33 0.09451893129259759 1107 34-35 0.011404827118482785 1107 36-37 -0.041856786603645446 1107 38-39 -0.01670454302735891 1107 40-41 -0.10339121238752824 1107 42-43 -0.12877613537921917 1107 44-45 -0.18214575352863704 1107 46-47 -0.036430718814990826 1107 48-49 0.15202245734008812 1107 50-51 0.22801650325025236 1107 52-53 0.15922806087067443 1107 54-55 0.39625366316947463 1107 56-57 0.4930871397371419 1107 58-59 0.2401460306193144 1107 60-61 0.22968311426980392 1107 62-63 0.2692909328631927 1107 64-65 0.4125212367899138 1107 66-67 0.3121001245841697 1107 68-69 0.22176125790498702 1107 70-71 0.5093213113374091 1107 72-73 0.2575135605115406 1107 74-75 0.42118451490748043 1107 76-77 0.3932341404482891 1107 78-79 0.5464518210687999 1107 80-81 0.4501636274065426 1107 82-83 0.503840009146213 1107 84-85 0.5812880809880276 1107 86-87 0.4780562725378026 1107 88-89 0.46442246192474457 1107 90-91 0.4648915904694313 1107 92-93 0.41692032676677826 1107 94-95 0.593182993796642 1107 96-97 0.6920181418615208 1107 98-99 0.8525208739327823 1107 100-101 0.8226853128122009 1108 1 -0.07928435929639832 1108 2 -0.1798684110255806 1108 3 -0.1517619157302157 1108 4 -0.14521625960114903 1108 5 -0.08228651245608631 1108 6 -0.05516847424883764 1108 7 -0.006761721179628921 1108 8 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-2.5102799115052328 2316 92-93 -2.4101445296771864 2316 94-95 -2.4662653301302733 2316 96-97 -2.2279720873088458 2316 98-99 -2.801911188053907 2316 100-101 -3.393989667031196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2202.0 3 319.0 4 284.0 5 275.0 6 313.0 7 400.0 8 470.0 9 489.0 10 729.0 11 1032.0 12 1732.0 13 2479.0 14 2923.0 15 4120.0 16 5842.0 17 8463.0 18 6730.0 19 3334.0 20 3024.0 21 2975.0 22 3049.0 23 3406.0 24 3930.0 25 4247.0 26 4979.0 27 5862.0 28 7169.0 29 8576.0 30 10887.0 31 13682.0 32 18229.0 33 24408.0 34 33965.0 35 49832.0 36 75880.0 37 131402.0 38 139432.0 39 20803.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.31717019657557 25.01390670212257 16.832804300926178 20.83611880037569 2 14.008872786546625 23.406470303882216 41.50807674349926 21.076580166071903 3 15.270827868270962 29.479825052010895 33.72285823174504 21.526488847973102 4 10.440253025048424 20.814840968411467 42.68989252704972 26.055013479490384 5 9.987774314841916 40.2385081034349 37.86943100242369 11.904286579299491 6 24.10571350847835 43.599897781697976 19.4110906857694 12.883298024054273 7 22.743008047792216 36.350403708380455 25.397856339820578 15.508731904006753 8 22.434829054545276 37.003644924059955 22.621114182892434 17.940411838502335 9 24.060056179034405 17.635073652560365 25.107478669110762 33.19739149929447 10-11 20.664277523172135 29.50921980202591 31.16612131869509 18.660381356106868 12-13 21.859074864520426 29.119658922857017 30.391196754095713 18.630069458526847 14-15 20.3676211782375 30.414268298010715 28.807048751556895 20.411061772194888 16-17 17.13762175716077 32.97715989393104 30.646824484224428 19.23839386468377 18-19 16.884680817392336 31.20404805707767 34.293584696642995 17.617686428887 20-21 17.270376814365417 30.645930983439424 34.544874610876256 17.538817591318903 22-23 18.139460127756028 30.08390686173501 33.82468576138471 17.951947249124252 24-25 17.6078491163151 31.045051653020273 33.96358122112932 17.383518009535308 26-27 17.20755245677364 30.924195223260643 34.3235425011126 17.54470981885312 28-29 17.105018058813542 29.82366487589614 35.129873448186615 17.9414436171037 30-31 17.857410761168918 30.00681712706317 34.430365973386 17.70540613838191 32-33 17.3054462280286 30.82255970656721 34.09128562302953 17.780708442374664 34-35 17.83689051156265 30.505561431290584 34.13003354252416 17.52751451462261 36-37 18.401500010302268 29.892980034203532 33.305008962973645 18.40051099252055 38-39 17.148595833903396 31.42613890175535 33.350448934244774 18.07481633009648 40-41 17.304042321205024 30.542110637414115 34.462129559654095 17.69171748172677 42-43 17.536389562006494 30.43049305177786 33.611839177093124 18.42127820912253 44-45 17.573585593040825 30.900138224120894 33.164145470790366 18.362130712047918 46-47 18.18130864077454 30.863088344750672 32.49248071031221 18.46312230416258 48-49 17.74024171306012 31.02761846198484 32.45330018612334 18.778839638831695 50-51 17.591180587268433 30.9820284847116 32.225988383882516 19.200802544137456 52-53 17.820663864084377 31.684624574068227 32.25366035439832 18.241051207449075 54-55 18.411239604025685 31.258561996686474 31.729708789080206 18.60048961020763 56-57 18.07927530271951 31.85483638353509 31.360941869118363 18.704946444627033 58-59 17.789291597887296 30.764095890873815 32.34285544753222 19.10375706370666 60-61 18.522907541626665 31.537127152410662 31.48323190203261 18.456733403930066 62-63 18.003669222705003 31.12946455062736 32.44517594466825 18.421690281999382 64-65 18.114099136666457 30.70503924393211 32.15249918413497 19.028362435266466 66-67 17.94979982891924 31.02453970540487 31.55937309944605 19.466287366229846 68-69 17.894110560184455 30.457490702597667 32.18742969779425 19.460969039423624 70-71 18.134728690731585 30.668879810311395 32.57911727545974 18.617274223497287 72-73 18.314573034948715 30.153258094344874 32.25606529869505 19.276103572011362 74-75 17.682256424119647 30.999128010958383 31.753505676170633 19.56510988875134 76-77 17.55307443979155 31.028241807963617 32.321198539901744 19.097485212343088 78-79 17.747611575206534 30.576287837329975 31.881762258602613 19.794338328860874 80-81 17.68639193219909 30.848821866008635 31.413472324215352 20.051313877576924 82-83 17.94486781477999 30.787507283187644 31.74571517812184 19.52190972391053 84-85 17.62303997995893 30.20837110262149 31.721504926213917 20.447083991205663 86-87 17.39902198796521 30.621962254075463 31.96594593080079 20.013069827158542 88-89 18.426367953798724 29.79810840455453 31.789417646704955 19.98610599494179 90-91 17.928027790025425 29.57972943459825 32.27005443449523 20.22218834088109 92-93 18.199925667161946 29.85468836658121 32.037041157482605 19.908344808774242 94-95 18.089499399661964 29.008729417717433 32.269096476835685 20.63267470578492 96-97 18.53449909765886 28.806190307455232 31.544610262791405 21.1147003320945 98-99 17.983578469548963 29.168943314155925 31.674697772470406 21.172780443824703 100-101 18.251929079744087 29.050902654400545 32.05082354647426 20.646344719381112 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1393.0 1 859.0 2 337.5 3 432.5 4 410.0 5 409.5 6 683.0 7 1252.5 8 1978.0 9 2232.5 10 1826.0 11 1418.5 12 1334.5 13 1291.0 14 1272.5 15 1252.0 16 1272.0 17 1378.5 18 1470.5 19 1565.0 20 1723.5 21 2023.5 22 2325.5 23 2611.0 24 2945.0 25 3702.5 26 5735.0 27 8032.5 28 8572.0 29 9488.5 30 12616.5 31 15655.5 32 19363.5 33 24702.0 34 30405.5 35 34170.0 36 38286.5 37 42473.0 38 40680.5 39 36175.0 40 32153.5 41 28336.5 42 24975.5 43 23065.0 44 21887.5 45 20893.5 46 18234.5 47 13929.5 48 11643.5 49 9880.5 50 7754.0 51 5492.5 52 3949.0 53 3430.5 54 3038.5 55 2639.5 56 2268.0 57 1896.0 58 1603.0 59 1285.0 60 1007.0 61 852.0 62 662.0 63 457.0 64 298.5 65 222.5 66 163.5 67 142.0 68 115.0 69 86.5 70 84.5 71 66.5 72 56.0 73 56.0 74 48.5 75 36.5 76 26.5 77 17.5 78 13.5 79 10.5 80 6.0 81 5.5 82 4.0 83 0.5 84 0.0 85 1.0 86 2.5 87 2.5 88 1.5 89 1.0 90 1.5 91 1.5 92 0.5 93 0.5 94 1.0 95 1.0 96 1.5 97 2.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3374054491555816 2 0.28805311627080615 3 0.28805311627080615 4 0.2908497484676101 5 0.291672287349023 6 0.2186308346795553 7 0.20530570480066596 8 0.21007643031286088 9 0.2043186581429704 10-11 0.22710298515810842 12-13 0.21797280357442497 14-15 0.21361334750293645 16-17 0.21180376196382805 18-19 0.2076088136686221 20-21 0.20111075650546 22-23 0.2054702125769485 24-25 0.19436593767787402 26-27 0.19477720711858051 28-29 0.20481218147181818 30-31 0.21608096414717526 32-33 0.20752655978048085 34-35 0.21887759634397919 36-37 0.19930117096635158 38-39 0.19987694818334062 40-41 0.19420142990159145 42-43 0.20464767369553558 44-45 0.2057169742413724 46-47 0.20851360643817635 48-49 0.21172150807568674 50-51 0.2143536324962081 52-53 0.2318737106703034 54-55 0.2087603681026002 56-57 0.19173381325735267 58-59 0.1914047977047875 60-61 0.18778562662657064 62-63 0.19839637819679734 64-65 0.19017098938266813 66-67 0.18803238829099453 68-69 0.18400194777207118 70-71 0.19666904654583023 72-73 0.19494171489486306 74-75 0.19987694818334062 76-77 0.1978206009798083 78-79 0.19708031598653666 80-81 0.19995920207148193 82-83 0.19222733658620042 84-85 0.18424870943649507 86-87 0.18638731052816868 88-89 0.1871275955214403 90-91 0.19436593767787402 92-93 0.18811464217913582 94-95 0.18630505664002736 96-97 0.18408420166021247 98-99 0.1925563521387656 100-101 0.18227461612110404 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 607874.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.033059439604866 #Duplication Level Percentage of deduplicated Percentage of total 1 73.6012974194881 17.688643557145937 2 10.753994808100892 5.169027928725817 3 3.986625835654217 2.8743244701522666 4 2.1567509429478853 2.0733329443316144 5 1.3797852251809006 1.6580230165330585 6 0.9378164664401784 1.3523159328838206 7 0.7145044995919259 1.202021037599052 8 0.5458095572054276 1.0493978824817962 9 0.4468948943212797 0.9666226402631324 >10 4.104664489632795 21.12954107059729 >50 0.7804914216663716 13.261242112410002 >100 0.561213761496715 24.997009084338735 >500 0.02740970752120708 4.234539812932609 >1k 0.002055728064090531 1.4525197818249762 >5k 6.85242688030177E-4 0.8914387277798861 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5413 0.8904805930176319 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4421 0.7272888789453078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2882 0.47411141124640965 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1517 0.24955829662068127 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 994 0.16352072962488937 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 957 0.15743394190243373 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 860 0.14147668760302298 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 843 0.13868005540621905 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 838 0.13785751652480613 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 812 0.13358031434145892 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 808 0.1329222832363286 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 802 0.13193523657863307 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 696 0.11449741229267907 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 684 0.11252331897728805 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 683 0.11235881120100547 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 683 0.11235881120100547 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 682 0.11219430342472288 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 651 0.10709456235996274 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 630 0.10363989905802846 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 629 0.10347539128174588 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 614 0.10100777463750713 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.225388814129244E-5 0.0 24-25 0.0 0.0 0.0 3.290155525651698E-4 0.0 26-27 0.0 0.0 0.0 0.0011515544339780943 0.0 28-29 0.0 0.0 0.0 0.002961139973086528 0.0 30-31 0.0 0.0 0.0 0.008307642702270536 0.0 32-33 0.0 0.0 0.0 0.01513471541799781 0.0 34-35 0.0 0.0 0.0 0.024758420330529024 0.0 36-37 0.0 0.0 0.0 0.03857707353826616 0.0 38-39 0.0 0.0 0.0 0.04820077845079737 0.0 40-41 0.0 0.0 0.0 0.057248706146339534 0.0 42-43 8.225388814129244E-5 0.0 0.0 0.06859974270983789 0.0 44-45 1.645077762825849E-4 0.0 0.0 0.0848037586736725 0.0 46-47 1.645077762825849E-4 0.0 0.0 0.10676554680739758 0.0 48-49 1.645077762825849E-4 0.0 0.0 0.13933808651134938 0.0 50-51 1.645077762825849E-4 0.0 0.0 0.17158161066273603 0.0 52-53 1.645077762825849E-4 0.0 0.0 0.2294060940260646 0.0 54-55 1.645077762825849E-4 0.0 0.0 0.3298380914465827 0.0 56-57 1.645077762825849E-4 0.0 0.0 0.494757137169874 0.0 58-59 1.645077762825849E-4 0.0 0.0 0.6814734632506079 0.0 60-61 1.645077762825849E-4 0.0 0.0 0.8600466544053538 0.0 62-63 1.645077762825849E-4 0.0 0.0 1.0757986029999638 0.0 64-65 1.645077762825849E-4 0.0 0.0 1.356284361561771 0.0 66-67 1.645077762825849E-4 0.0 0.0 1.7312798375979233 0.0 68-69 1.645077762825849E-4 0.0 0.0 2.180550574625663 0.0 70-71 1.645077762825849E-4 0.0 0.0 2.6271891872328803 0.0 72-73 1.645077762825849E-4 0.0 0.0 3.0553206750083075 0.0 74-75 1.645077762825849E-4 0.0 0.0 3.545965117771117 0.0 76-77 1.645077762825849E-4 0.0 0.0 4.121331065319458 0.0 78-79 1.645077762825849E-4 0.0 0.0 4.6921730490200275 0.0 80-81 1.645077762825849E-4 0.0 0.0 5.335974231501924 0.0 82-83 1.645077762825849E-4 0.0 0.0 5.9847929011604375 0.0 84-85 1.645077762825849E-4 0.0 0.0 6.711588256776897 0.0 86-87 1.645077762825849E-4 0.0 0.0 7.5201439772058025 0.0 88-89 1.645077762825849E-4 0.0 0.0 8.492960712252868 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 20 0.0020643952 71.32997 1 GGTATCA 2060 0.0 59.326225 1 GTATCAA 3810 0.0 39.066113 1 GTATGGT 100 2.996967E-8 38.042652 1 TACAGGG 55 0.00257284 34.561394 2 CATTCCG 245 0.0 32.95807 9 TCAACGC 4500 0.0 32.211956 4 ATCAACG 4525 0.0 32.03135 3 GATCCTC 90 2.402148E-5 31.683891 5 ATACTCG 120 1.5042679E-7 31.665596 6 CGCAGAA 60 0.003950146 31.665596 9 TACTCGC 120 1.5042679E-7 31.665596 7 CAACGCA 4595 0.0 31.545988 5 TAGGCAT 250 0.0 30.416536 5 AACGCAG 4765 0.0 30.402956 6 ACTCGCC 125 2.1517008E-7 30.39897 8 TATCAAC 4850 0.0 30.080881 2 ATGGGTA 195 1.6370905E-11 29.246668 5 GGCATTC 285 0.0 28.332375 7 GCCTATG 85 6.4049265E-4 27.94023 9 >>END_MODULE