FastQCFastQC Report
Thu 26 May 2016
SRR937174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607874
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55310.9098925106189769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49830.8197422492161205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34690.570677475924287No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20390.33543135584019057No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11630.1913225438166462No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11370.187045341633299No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.1674689162556714No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT10010.16467228405886747No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8390.13802202430108873No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA8060.1325932676837634No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG8010.13177072880235047No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7240.11910363002859145No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC7230.11893912225230886No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT7200.11844559892346111No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG7010.115319951174092No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6980.11482642784524424No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC6970.11466192006896166No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC6920.11383938118754873No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG6680.10989119455676671No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA6490.10676554680739758No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG6490.10676554680739758No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT6220.10232383684776779No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT6200.10199482129520261No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA6200.10199482129520261No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG6110.10051425130865935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.058.9592671
CGCAGAA804.2145984E-947.5051429
GGATAGC608.272829E-539.594131
GTATCAA39200.037.5740241
GTGGTAC1351.1919838E-831.6753041
GGATGAC1657.6397555E-1131.6700958
GTTGTGC753.0748008E-431.6700958
TAGGACG1401.702756E-830.5365074
GGACGTG1401.7040293E-830.5339956
TGGTACT1252.1505912E-730.4007912
TCAACGC50050.029.4213434
GGTACTC1303.030018E-729.2339348
CAACGCA50600.029.1954215
ATCAACG50350.029.1517013
AACGCAG51200.029.0364536
CTGCTCG1004.9456714E-528.5030849
CGATATC504.952465E-528.5007480-81
GTTCCGA504.953854E-528.49956976-77
TATCAAC51950.028.1624262
GCAGATC1554.575122E-827.583638
Produced by FastQC (version 0.11.5)