##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937174_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 607874 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.021649223358786 31.0 31.0 34.0 30.0 34.0 2 32.26513553795688 33.0 31.0 34.0 31.0 34.0 3 32.30495629028384 34.0 31.0 34.0 30.0 34.0 4 35.99987332901226 37.0 35.0 37.0 35.0 37.0 5 35.850653260379616 37.0 35.0 37.0 35.0 37.0 6 35.948953237019516 37.0 35.0 37.0 35.0 37.0 7 35.90619931104143 37.0 35.0 37.0 35.0 37.0 8 35.887060476348715 37.0 35.0 37.0 35.0 37.0 9 37.54816787689554 39.0 37.0 39.0 35.0 39.0 10-11 37.47763928050879 39.0 37.0 39.0 35.0 39.0 12-13 37.426418961824325 39.0 37.0 39.0 34.0 39.0 14-15 38.559744289112544 40.0 38.0 41.0 34.0 41.0 16-17 38.52364305760733 40.0 38.0 41.0 34.0 41.0 18-19 38.61221075420235 40.0 38.0 41.0 34.0 41.0 20-21 38.580050306477986 40.0 38.0 41.0 34.0 41.0 22-23 38.45187160497077 40.0 38.0 41.0 34.0 41.0 24-25 38.30027851166525 40.0 38.0 41.0 34.0 41.0 26-27 38.0787794839062 40.0 37.0 41.0 33.0 41.0 28-29 38.05660301312443 40.0 37.0 41.0 33.0 41.0 30-31 38.056675396546 40.0 37.0 41.0 34.0 41.0 32-33 37.93201798399011 40.0 37.0 41.0 33.0 41.0 34-35 37.80217199617026 40.0 37.0 41.0 33.0 41.0 36-37 37.60044515804262 40.0 36.0 41.0 33.0 41.0 38-39 37.44217140394227 40.0 36.0 41.0 33.0 41.0 40-41 37.282017325958996 40.0 36.0 41.0 33.0 41.0 42-43 37.03075061608162 40.0 36.0 41.0 32.0 41.0 44-45 36.6557181257958 40.0 36.0 41.0 31.0 41.0 46-47 36.211863807302166 39.0 35.5 41.0 30.0 41.0 48-49 35.82909862899219 39.0 35.0 41.0 28.5 41.0 50-51 35.63273638944913 39.0 35.0 41.0 28.0 41.0 52-53 35.45401760891237 39.0 35.0 40.5 27.0 41.0 54-55 35.26408844596084 39.0 35.0 40.0 26.5 41.0 56-57 35.051172611429344 39.0 35.0 40.0 26.0 41.0 58-59 34.83879060463188 38.5 35.0 40.0 26.0 41.0 60-61 34.55865113493915 38.0 34.5 40.0 24.5 41.0 62-63 34.21126746661315 38.0 34.0 40.0 23.0 41.0 64-65 33.899181409305214 38.0 34.0 40.0 22.0 41.0 66-67 33.522388685813176 37.0 33.5 39.5 21.5 41.0 68-69 33.07971388807549 36.5 33.0 39.0 20.0 40.5 70-71 32.722215459124754 36.0 32.5 39.0 18.5 40.0 72-73 32.28035086218526 36.0 32.0 38.5 17.5 40.0 74-75 31.784070383007005 35.0 31.5 37.5 13.0 39.5 76-77 30.390969181113192 34.0 30.0 36.0 9.0 39.0 78-79 30.98596913176086 35.0 31.0 36.5 7.5 39.0 80-81 30.758550291672286 35.0 31.0 36.0 4.5 38.5 82-83 30.3791221536042 34.5 31.0 36.0 2.0 37.0 84-85 29.983736761236703 34.0 31.0 35.0 2.0 37.0 86-87 29.63779089087541 34.0 30.0 35.0 2.0 36.5 88-89 29.226872674271313 34.0 30.0 35.0 2.0 36.0 90-91 28.778162086221815 34.0 29.0 35.0 2.0 36.0 92-93 28.36732776858362 34.0 29.0 35.0 2.0 35.0 94-95 27.930136837568313 34.0 29.0 35.0 2.0 35.0 96-97 27.49972198185808 33.5 28.0 35.0 2.0 35.0 98-99 26.887078572204107 33.0 26.5 35.0 2.0 35.0 100-101 25.253154436610217 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06999334793052725 1101 2 0.10307339294023166 1101 3 0.17243313541847272 1101 4 -0.04396838871465292 1101 5 0.01878455501307741 1101 6 0.03685038205784252 1101 7 -0.01098330593806196 1101 8 0.09299691079407069 1101 9 0.07954226176915569 1101 10-11 0.18896750643334315 1101 12-13 0.21609164559692573 1101 14-15 0.3418330135351937 1101 16-17 0.1564317774466204 1101 18-19 0.3118822660534164 1101 20-21 0.27848078449803637 1101 22-23 0.10085205855281743 1101 24-25 0.12274554032453011 1101 26-27 0.11757630481119463 1101 28-29 0.1728311247359997 1101 30-31 0.41090348441091606 1101 32-33 0.37031289011436996 1101 34-35 0.35636131708675833 1101 36-37 0.31360677568835627 1101 38-39 0.2567093982703028 1101 40-41 0.38329272277730553 1101 42-43 0.4810960644481028 1101 44-45 0.45799059972427614 1101 46-47 0.41885037217060983 1101 48-49 0.33957368460266224 1101 50-51 0.40207656274740344 1101 52-53 0.5589003244440747 1101 54-55 0.5113734548038025 1101 56-57 0.494947148179925 1101 58-59 0.35102051185974403 1101 60-61 0.5865306095531082 1101 62-63 0.6882197433901567 1101 64-65 0.5948113656369927 1101 66-67 0.46030177634797553 1101 68-69 0.4342334827142409 1101 70-71 0.31797385653784715 1101 72-73 0.30631952896301584 1101 74-75 0.2980616988932674 1101 76-77 0.3672340558956755 1101 78-79 0.19106061013638964 1101 80-81 0.18256795869858067 1101 82-83 0.2429888011035466 1101 84-85 0.09746342954018417 1101 86-87 0.20417017702419926 1101 88-89 0.5885592457037312 1101 90-91 0.7579581131766773 1101 92-93 0.8873901768336196 1101 94-95 0.6458569560343967 1101 96-97 0.4084091363913842 1101 98-99 0.3493231382212052 1101 100-101 1.0112440167912027 1102 1 0.17868380591660582 1102 2 0.11309422786645484 1102 3 0.051098362117762974 1102 4 0.02031404878056975 1102 5 -0.07307548780364925 1102 6 0.04234583278037718 1102 7 0.08818807566603226 1102 8 0.08133699795669713 1102 9 0.18804157364314733 1102 10-11 0.1651196396239527 1102 12-13 0.19011727829055047 1102 14-15 0.1639070444244055 1102 16-17 0.2520502306922765 1102 18-19 0.1479888108962939 1102 20-21 0.2351455715612687 1102 22-23 0.14979740987937618 1102 24-25 -0.09152925132621448 1102 26-27 0.02548449733356506 1102 28-29 0.07330134417173184 1102 30-31 0.11560902382524318 1102 32-33 0.23184664651777354 1102 34-35 0.2541531016268621 1102 36-37 0.22295095337778292 1102 38-39 0.26477815352682654 1102 40-41 0.3177463316159006 1102 42-43 0.3211880057280183 1102 44-45 0.31428695265645956 1102 46-47 0.3529049635002224 1102 48-49 0.5311450967901337 1102 50-51 0.5372909331121107 1102 52-53 0.6406176766881302 1102 54-55 0.5491941024068225 1102 56-57 0.5326077109140854 1102 58-59 0.3626111342314857 1102 60-61 0.524300603436437 1102 62-63 0.5824801035094325 1102 64-65 0.7744815430205776 1102 66-67 0.6284410645198548 1102 68-69 0.21019112473580037 1102 70-71 0.6150698692299486 1102 72-73 0.5670882230527852 1102 74-75 0.5435960478496149 1102 76-77 0.4184182060600641 1102 78-79 0.421826532607529 1102 80-81 0.48528366708606896 1102 82-83 0.4169604350205134 1102 84-85 0.4672833699025958 1102 86-87 0.5679451215918299 1102 88-89 0.7015504912117976 1102 90-91 0.46256569020856375 1102 92-93 0.5611324354160772 1102 94-95 0.6530607751337172 1102 96-97 0.42585838709862145 1102 98-99 0.4063157408666669 1102 100-101 0.645051073905659 1103 1 -0.036091908549842344 1103 2 0.06478134802426894 1103 3 -0.01172642198427809 1103 4 -0.060839218857928756 1103 5 -0.17720554670408006 1103 6 -0.028386228828445326 1103 7 -0.01958050233603359 1103 8 -0.005183846798260561 1103 9 0.03902629326866247 1103 10-11 0.035736664988156974 1103 12-13 -0.06978849039053614 1103 14-15 0.05925686238130368 1103 16-17 -0.017962795815257948 1103 18-19 -0.14756530768240594 1103 20-21 -0.1678385763973651 1103 22-23 -0.1562856879928063 1103 24-25 -0.29000191674646203 1103 26-27 -0.1570201533170632 1103 28-29 -0.19301341935966576 1103 30-31 -0.17910048037088444 1103 32-33 0.07475891439524673 1103 34-35 -0.09603737590241224 1103 36-37 -0.19038762804491682 1103 38-39 -0.00623880436194213 1103 40-41 0.08721580601037004 1103 42-43 -0.1573545717398659 1103 44-45 0.022338552620851715 1103 46-47 0.06304994196899827 1103 48-49 -0.021570086588418746 1103 50-51 -0.0730079736921283 1103 52-53 0.07029679350966234 1103 54-55 -0.011769249997691134 1103 56-57 -0.18221251859610987 1103 58-59 -0.2828968292755434 1103 60-61 -0.6037504342326656 1103 62-63 -0.33432596553105753 1103 64-65 -0.621530143349851 1103 66-67 -0.5837562917551296 1103 68-69 -0.4898067666568622 1103 70-71 -0.5387870078757331 1103 72-73 -0.37943163768541766 1103 74-75 -0.300755930258358 1103 76-77 -0.7243637967420398 1103 78-79 -0.22186685714276422 1103 80-81 -0.11627974810808794 1103 82-83 -0.1334786238846064 1103 84-85 -0.07736521897052029 1103 86-87 0.06447997687107687 1103 88-89 0.11628309637272949 1103 90-91 0.0063790362872566675 1103 92-93 -0.2754165241033455 1103 94-95 -0.22046495690446122 1103 96-97 0.11030881374017198 1103 98-99 0.32999938416527996 1103 100-101 0.7664096261827247 1104 1 0.08088381156488822 1104 2 0.18933190755240759 1104 3 0.0631668569448749 1104 4 0.20460335375953065 1104 5 -0.012074047491864803 1104 6 0.10783294999163218 1104 7 0.15562384999836354 1104 8 0.11138114869050497 1104 9 0.019129715076118714 1104 10-11 0.10781547025081295 1104 12-13 -0.041189263360820405 1104 14-15 0.2316534742397991 1104 16-17 0.23951104573929882 1104 18-19 0.1540277652111044 1104 20-21 0.11402288147306194 1104 22-23 0.15730256393624842 1104 24-25 -0.024896003658483323 1104 26-27 0.02811565513713532 1104 28-29 -0.03362533172045801 1104 30-31 -0.017443307502944094 1104 32-33 0.163307103840296 1104 34-35 0.25760326024943225 1104 36-37 0.2911162974051891 1104 38-39 0.16215829228627854 1104 40-41 0.23357516985853266 1104 42-43 0.10090031992309179 1104 44-45 0.24217722537431996 1104 46-47 0.2029454428510462 1104 48-49 -0.019637660889166852 1104 50-51 -0.039921075134380146 1104 52-53 0.15947116967955566 1104 54-55 0.23465542579918974 1104 56-57 0.2681975327108006 1104 58-59 0.18494208587278393 1104 60-61 0.09404040593485519 1104 62-63 -0.005338906723373782 1104 64-65 0.29857381711286024 1104 66-67 0.18237979949307004 1104 68-69 0.18610120409415742 1104 70-71 0.271804974240915 1104 72-73 0.2617162366463148 1104 74-75 0.40003791291216473 1104 76-77 0.5973345832523016 1104 78-79 0.18436336410987764 1104 80-81 0.22099586831550866 1104 82-83 0.31765187881540413 1104 84-85 0.4173290268448042 1104 86-87 0.3261351297818358 1104 88-89 0.378692366629533 1104 90-91 0.7234739338754252 1104 92-93 0.7006638163267702 1104 94-95 0.6645456142047621 1104 96-97 0.527167376338646 1104 98-99 0.7601829309407542 1104 100-101 1.15816685426282 1105 1 0.03128023497480825 1105 2 0.017755533577236804 1105 3 0.053619828657218704 1105 4 0.06938042468352279 1105 5 -0.01930691754332514 1105 6 -0.037659619039168035 1105 7 -0.08586126330084909 1105 8 0.02593288183294362 1105 9 0.018285542567781476 1105 10-11 -0.06545825324779742 1105 12-13 -0.008771913477197302 1105 14-15 0.10380073394327383 1105 16-17 0.19490834708862081 1105 18-19 0.04944717262451803 1105 20-21 -0.08565993477647282 1105 22-23 -0.05948431289560574 1105 24-25 -0.1190268661551599 1105 26-27 -0.07626008492076153 1105 28-29 -0.020197361021118354 1105 30-31 -0.004773966497772619 1105 32-33 -0.18613754320555387 1105 34-35 -0.275423019403533 1105 36-37 -0.12248996252790079 1105 38-39 -0.050064425183819594 1105 40-41 -0.02200424763993425 1105 42-43 -0.3355177323120486 1105 44-45 -0.20363590409698418 1105 46-47 -0.3650655966742349 1105 48-49 -0.5597639941189527 1105 50-51 -0.422297454512254 1105 52-53 -0.659198324402233 1105 54-55 -0.6513306352243546 1105 56-57 -0.7891859377161481 1105 58-59 -0.8187981611208386 1105 60-61 -0.6047712242860754 1105 62-63 -0.7582586180388873 1105 64-65 -0.5495269072997147 1105 66-67 -0.4745642510042032 1105 68-69 -0.4484227501449425 1105 70-71 -0.6658692066866401 1105 72-73 -0.6347994694645962 1105 74-75 -0.5266642765336549 1105 76-77 -0.47898536488173704 1105 78-79 -0.687584670881634 1105 80-81 -0.5794226161359539 1105 82-83 -0.43587451114277087 1105 84-85 -0.48569468652373615 1105 86-87 -0.3033237894072194 1105 88-89 -0.31848494561524276 1105 90-91 -0.5297676885566034 1105 92-93 -0.6066813849464694 1105 94-95 -0.23204212796019164 1105 96-97 -0.13544050890791937 1105 98-99 -0.023321713133608313 1105 100-101 0.329288500646836 1106 1 0.03553667605382316 1106 2 0.031049929598296444 1106 3 -0.061779997510171825 1106 4 -0.018896599248620305 1106 5 -0.0673968815032211 1106 6 -0.047484065981123535 1106 7 0.04332451638754975 1106 8 -0.05399883636161462 1106 9 -0.2428901289109362 1106 10-11 -0.023135152503087397 1106 12-13 -0.06561271811214198 1106 14-15 0.0023108678480099343 1106 16-17 0.04763325140248753 1106 18-19 0.150635401163008 1106 20-21 -0.1109282246255816 1106 22-23 -0.0699466674113367 1106 24-25 0.0854968034556407 1106 26-27 0.2925074687092746 1106 28-29 0.220893717880557 1106 30-31 0.2623663083722363 1106 32-33 0.03380203990899844 1106 34-35 0.12400766763744997 1106 36-37 0.30545776256633417 1106 38-39 0.41362958474117306 1106 40-41 0.3641901365390723 1106 42-43 0.036139955982399385 1106 44-45 0.04130272941785762 1106 46-47 0.11603513462926429 1106 48-49 -0.2019347120386854 1106 50-51 0.052887829149931065 1106 52-53 0.05699313277528262 1106 54-55 0.020402797612128154 1106 56-57 0.08433184884511746 1106 58-59 0.028498384347116712 1106 60-61 0.29294236303353216 1106 62-63 0.21226580678298035 1106 64-65 0.08931015702952294 1106 66-67 0.1087458078262884 1106 68-69 0.3356729069445521 1106 70-71 0.4519191420599995 1106 72-73 0.41241686275677125 1106 74-75 0.1630009504653671 1106 76-77 0.28432858172171294 1106 78-79 0.4608958578554869 1106 80-81 0.25840921310590304 1106 82-83 0.3198716843138989 1106 84-85 0.11765080913379933 1106 86-87 0.47778118716559703 1106 88-89 0.3867604197795558 1106 90-91 0.47897198035708044 1106 92-93 0.22153379211477642 1106 94-95 0.1598956587351381 1106 96-97 0.4045186471325408 1106 98-99 0.32651975957266544 1106 100-101 0.4483169149046837 1107 1 -0.0742284012396297 1107 2 -0.07903261081211355 1107 3 -0.03694953163304859 1107 4 -0.05269980738978575 1107 5 -0.07160547240790294 1107 6 -0.06440881840398305 1107 7 0.048071030907081536 1107 8 -0.04829331867627218 1107 9 0.0019246401787356149 1107 10-11 0.04903479608552175 1107 12-13 0.0730470143369999 1107 14-15 0.11733723492355352 1107 16-17 0.030191519112079845 1107 18-19 0.06371333835821957 1107 20-21 -0.031516148681348 1107 22-23 0.0693314567343748 1107 24-25 -0.086808507878672 1107 26-27 -0.009142016735921743 1107 28-29 -0.173322721417847 1107 30-31 -0.12024729492232211 1107 32-33 0.06301746508911776 1107 34-35 0.24978479089250527 1107 36-37 -0.07390404838438513 1107 38-39 0.09185426044378175 1107 40-41 0.23029896465924793 1107 42-43 0.23417190511775487 1107 44-45 0.17919856624180852 1107 46-47 0.08070633984271325 1107 48-49 -0.02132796065640008 1107 50-51 -0.0953211497652191 1107 52-53 0.14242157570688363 1107 54-55 -0.1699703538265993 1107 56-57 0.0932748289419365 1107 58-59 0.002327518642829318 1107 60-61 -0.0510388449136272 1107 62-63 -0.07630706422998657 1107 64-65 -0.257390431159088 1107 66-67 0.12126024798890711 1107 68-69 -0.022885149122963355 1107 70-71 -0.06145950325433347 1107 72-73 0.23322546777092867 1107 74-75 0.17059471231511836 1107 76-77 0.12979900033210612 1107 78-79 0.10759798926757824 1107 80-81 0.08003241965975505 1107 82-83 0.018064569612711523 1107 84-85 0.14710984316408116 1107 86-87 0.14567508393717432 1107 88-89 -0.08679975270873896 1107 90-91 0.015453783547584976 1107 92-93 0.17108912329053538 1107 94-95 0.2159653771629877 1107 96-97 0.25187951451232493 1107 98-99 0.33640190139049153 1107 100-101 0.42130499240095887 1108 1 -0.07346968233015261 1108 2 -0.15182300093774614 1108 3 -0.18005917711209918 1108 4 -0.050694924461389235 1108 5 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-1.1544911454787368 2316 78-79 -0.8992023031740359 2316 80-81 -1.087694461737403 2316 82-83 -1.0373370593144102 2316 84-85 -1.1777787830436068 2316 86-87 -1.1419016136590656 2316 88-89 -1.1281822909379606 2316 90-91 -1.300697466162024 2316 92-93 -1.6461604906591987 2316 94-95 -1.5661758694035548 2316 96-97 -1.3283659861231243 2316 98-99 -1.9032345280887135 2316 100-101 -1.9554271092156696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 13.0 6 29.0 7 72.0 8 189.0 9 368.0 10 699.0 11 1074.0 12 1634.0 13 2386.0 14 3153.0 15 4528.0 16 6371.0 17 9049.0 18 7522.0 19 3871.0 20 3031.0 21 2948.0 22 3187.0 23 3555.0 24 3855.0 25 4545.0 26 5577.0 27 6643.0 28 8356.0 29 10301.0 30 12909.0 31 16037.0 32 20435.0 33 26721.0 34 36308.0 35 51127.0 36 76273.0 37 132311.0 38 130031.0 39 12760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.181713296323665 25.6074418727133 17.423455658347994 19.78738917261504 2 14.613225767182014 24.191526533459236 40.79282877701629 20.40241892234246 3 15.839631239368684 29.676873825825744 33.870835074373964 20.612659860431602 4 11.064628524990376 21.61813138907076 42.0726992764948 25.24454080944406 5 10.147021079163677 40.572125224578976 37.704918032786885 11.57593566347046 6 24.889039504897397 43.62252703685303 18.78020774041989 12.708225717829682 7 23.05708090821453 36.69790120979018 24.99218588062658 15.252832001368704 8 23.220108114510573 37.32632091518966 22.033678031960573 17.419892938339196 9 24.646555042656868 17.781974553937165 24.933456604493696 32.638013798912276 10-11 21.251196794072456 29.726061650934238 30.696904292665916 18.32583726232739 12-13 22.32925773962484 29.084258183816296 30.325526531391173 18.26095754516769 14-15 20.72452514830376 30.398404932601164 28.782691807841758 20.09437811125332 16-17 17.32151728812221 33.041798135797876 30.734576573434623 18.902108002645285 18-19 17.054438913327434 31.096575935144454 34.563577320299935 17.285407831228184 20-21 17.546152656636078 30.366572677890485 35.06006178912077 17.027212876352664 22-23 18.325778270207223 29.989611274111837 34.31594393871721 17.368666516963728 24-25 18.045193799342293 30.68907712327122 34.430002418276246 16.83572665911024 26-27 17.41663883711404 30.639432639047687 34.87169068233676 17.07223784150151 28-29 17.11397361234495 29.77091764551048 35.67499095186392 17.440117790280656 30-31 18.092602036619674 29.80949874150723 35.03010512116077 17.067794100712323 32-33 17.706383230735316 30.567025403290813 34.53725607609472 17.18933528987915 34-35 18.110496583173486 30.078437307731537 34.892757380641385 16.918308728453592 36-37 18.62247541838509 29.660636350027065 33.94837408471835 17.768514146869492 38-39 17.524480873867464 31.117380700722602 33.9067814385418 17.451356986868134 40-41 17.630724155601456 30.291828809777034 34.94542861302557 17.13201842159594 42-43 17.788546965798187 30.28681943521267 34.095201742667044 17.829431856322103 44-45 17.810269891457768 30.668362193480885 33.67943027666917 17.841937638392167 46-47 18.520367707781546 30.771426315321925 32.907066267022444 17.801139709874086 48-49 18.044311887515978 31.031646412984294 32.735291746795795 18.188749952703937 50-51 18.106630650430848 30.826371912600308 32.376693196287384 18.690304240681456 52-53 18.233675647442517 31.563773653192072 32.399721981172036 17.80282871819337 54-55 18.952627479237044 31.11313455842375 31.786712202456595 18.14752575988261 56-57 18.540437656488024 31.7030338524102 31.577267657442164 18.179260833659608 58-59 18.11329321537029 30.812141989951865 32.35111223707545 18.7234525576024 60-61 18.774861237690704 31.501429572575894 31.503074650338718 18.220634539394677 62-63 18.45579840559063 30.929929557770198 32.60017701036728 18.014095026271892 64-65 18.566265377364388 30.583887450359775 32.262689307323555 18.587157864952275 66-67 18.253453840763054 31.22949821838078 31.494849261524593 19.022198679331574 68-69 18.26085669069577 30.36649042400234 32.235792285901354 19.136860599400535 70-71 18.434330140785754 30.864455462809726 32.48164915755567 18.21956523884884 72-73 18.740643620223928 30.113230702415304 32.227402389310946 18.918723288049826 74-75 18.174490108147413 31.083333059153706 31.545517656619626 19.196659176079255 76-77 18.06106199640057 30.957895879738235 32.360982703652404 18.620059420208793 78-79 18.212080135027982 30.502291593323616 31.70426766073232 19.58136061091608 80-81 17.983743341547758 30.86544250946742 31.31084731375252 19.8399668352323 82-83 18.207975047337346 30.80160099758008 31.40485399040257 19.585569964680005 84-85 17.696706384392794 30.54717021483106 31.415772702521743 20.340350698254404 86-87 17.41643827503726 30.878109608241182 31.72441986332694 19.981032253394616 88-89 18.28059762384968 30.089294820966188 31.584176984046035 20.045930571138097 90-91 17.873474575225497 29.886521147209915 31.9525025809344 20.287501696630184 92-93 18.078279988780245 30.224238780041013 31.503405820288176 20.194075410890562 94-95 17.91744670770587 29.418596617062086 31.689215199202465 20.97474147602957 96-97 18.72172522595143 29.270539618407764 30.837476187499384 21.17025896814142 98-99 17.90372000246766 29.737481749573302 30.950256020070327 21.40854222788871 100-101 17.879550938177903 29.80501003207467 31.11284047972978 21.202598550017644 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1243.0 1 779.5 2 353.5 3 428.0 4 405.0 5 422.5 6 676.5 7 1266.5 8 1848.0 9 2075.0 10 1869.0 11 1456.5 12 1333.5 13 1344.0 14 1317.5 15 1262.0 16 1257.5 17 1325.5 18 1399.5 19 1547.5 20 1788.5 21 2037.0 22 2290.0 23 2595.5 24 2911.5 25 3730.0 26 5886.0 27 8232.5 28 8691.0 29 9614.0 30 12537.0 31 15196.5 32 19036.5 33 24902.0 34 30305.0 35 33888.0 36 38516.0 37 42271.5 38 40378.0 39 36324.0 40 32452.5 41 28651.0 42 25092.0 43 23133.5 44 22204.0 45 20872.5 46 18153.0 47 14233.5 48 11731.5 49 9867.0 50 7810.0 51 5583.0 52 4046.0 53 3480.5 54 3029.0 55 2555.0 56 2132.0 57 1829.0 58 1559.0 59 1252.5 60 957.5 61 786.5 62 612.5 63 417.5 64 273.0 65 192.5 66 148.5 67 131.0 68 99.0 69 72.5 70 72.5 71 66.0 72 50.5 73 53.5 74 50.0 75 35.0 76 24.0 77 16.5 78 13.5 79 7.5 80 4.0 81 1.5 82 1.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046884716240536695 2 0.0 3 0.0 4 0.0 5 0.011515544339780942 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004606217735912376 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.75777216989047E-4 24-25 4.935233288477546E-4 26-27 0.001151554433978094 28-29 0.002303108867956188 30-31 6.580311051303395E-4 32-33 0.0 34-35 0.0 36-37 1.645077762825849E-4 38-39 2.467616644238773E-4 40-41 8.225388814129244E-5 42-43 0.011515544339780942 44-45 0.0 46-47 0.0 48-49 1.645077762825849E-4 50-51 0.0 52-53 2.467616644238773E-4 54-55 8.225388814129244E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.935233288477546E-4 84-85 1.645077762825849E-4 86-87 0.0 88-89 0.0 90-91 0.00847215047855312 92-93 0.0028788860849452355 94-95 0.0 96-97 0.0 98-99 0.001891839427249726 100-101 0.012091321556769988 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 607874.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.166163767449316 #Duplication Level Percentage of deduplicated Percentage of total 1 72.45499494951525 16.785042787701833 2 11.166397415341596 5.173651824324524 3 4.292312786670006 2.983092628713423 4 2.229103456558938 2.065591029169268 5 1.4168512276747913 1.6411503787212918 6 1.0035995361976433 1.394973072749447 7 0.7104635603953683 1.1521100633646955 8 0.578271375834309 1.0717063515684668 9 0.4741863207679126 0.9886570166874344 >10 4.24576128124997 21.101707995079053 >50 0.8115584358751149 13.325735162141832 >100 0.5830406657734378 25.070892544105362 >500 0.02776384122730656 3.891084322362991 >1k 0.004983253553619126 2.4424412483765323 >5k 7.118933648027323E-4 0.9121635749338689 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5531 0.9098925106189769 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4983 0.8197422492161205 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3469 0.570677475924287 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2039 0.33543135584019057 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1163 0.1913225438166462 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1137 0.187045341633299 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1018 0.1674689162556714 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1001 0.16467228405886747 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 839 0.13802202430108873 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 806 0.1325932676837634 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 801 0.13177072880235047 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 724 0.11910363002859145 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 723 0.11893912225230886 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 720 0.11844559892346111 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 701 0.115319951174092 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 698 0.11482642784524424 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 697 0.11466192006896166 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 692 0.11383938118754873 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 668 0.10989119455676671 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 649 0.10676554680739758 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 649 0.10676554680739758 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 622 0.10232383684776779 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 620 0.10199482129520261 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 620 0.10199482129520261 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 611 0.10051425130865935 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.225388814129244E-5 0.0 24-25 0.0 0.0 0.0 3.290155525651698E-4 0.0 26-27 0.0 0.0 0.0 0.0013160622102606789 0.0 28-29 0.0 0.0 0.0 0.0031256477493691124 0.0 30-31 0.0 0.0 0.0 0.008472150478553122 0.0 32-33 0.0 0.0 0.0 0.01554598485870427 0.0 34-35 0.0 0.0 0.0 0.02459391255424644 0.0 36-37 0.0 0.0 0.0 0.038165804097559694 0.0 38-39 0.0 0.0 0.0 0.047707255121949614 0.0 40-41 0.0 0.0 0.0 0.056919690593774365 0.0 42-43 0.0 0.0 0.0 0.06859974270983789 0.0 44-45 0.0 0.0 0.0 0.08513277422623768 0.0 46-47 0.0 0.0 0.0 0.10701230847182147 0.0 48-49 0.0 0.0 0.0 0.13991386372833842 0.0 50-51 0.0 0.0 0.0 0.17149935677459474 0.0 52-53 0.0 0.0 0.0 0.23014637901933624 0.0 54-55 0.0 0.0 0.0 0.3312364075449846 0.0 56-57 0.0 0.0 0.0 0.4965667227089824 0.0 58-59 0.0 0.0 0.0 0.6846813648881183 0.0 60-61 0.0 0.0 0.0 0.8653931571345378 0.0 62-63 0.0 0.0 0.0 1.0817208829461369 0.0 64-65 0.0 0.0 0.0 1.3627001648367918 0.0 66-67 0.0 0.0 0.0 1.7390939569713462 0.0 68-69 0.0 0.0 0.0 2.191737103412878 0.0 70-71 0.0 0.0 0.0 2.6398562860066397 0.0 72-73 0.0 0.0 0.0 3.067494250453219 0.0 74-75 0.0 0.0 0.0 3.560277294307702 0.0 76-77 0.0 0.0 0.0 4.138439874052846 0.0 78-79 0.0 0.0 0.0 4.714463852706317 0.0 80-81 0.0 0.0 0.0 5.3612261751613 0.0 82-83 0.0 0.0 0.0 6.014157539226879 0.0 84-85 0.0 0.0 0.0 6.747450952006501 0.0 86-87 0.0 0.0 0.0 7.560283874618753 0.0 88-89 0.0 0.0 0.0 8.538364858506862 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2200 0.0 58.959267 1 CGCAGAA 80 4.2145984E-9 47.505142 9 GGATAGC 60 8.272829E-5 39.59413 1 GTATCAA 3920 0.0 37.574024 1 GTGGTAC 135 1.1919838E-8 31.675304 1 GGATGAC 165 7.6397555E-11 31.670095 8 GTTGTGC 75 3.0748008E-4 31.670095 8 TAGGACG 140 1.702756E-8 30.536507 4 GGACGTG 140 1.7040293E-8 30.533995 6 TGGTACT 125 2.1505912E-7 30.400791 2 TCAACGC 5005 0.0 29.421343 4 GGTACTC 130 3.030018E-7 29.233934 8 CAACGCA 5060 0.0 29.195421 5 ATCAACG 5035 0.0 29.151701 3 AACGCAG 5120 0.0 29.036453 6 CTGCTCG 100 4.9456714E-5 28.503084 9 CGATATC 50 4.952465E-5 28.50074 80-81 GTTCCGA 50 4.953854E-5 28.499569 76-77 TATCAAC 5195 0.0 28.162426 2 GCAGATC 155 4.575122E-8 27.58363 8 >>END_MODULE