FastQCFastQC Report
Thu 26 May 2016
SRR937173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643478
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55820.8674733246513479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46490.7224800226270361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30440.47305424583280237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17720.2753784900183068No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.15975682152303575No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA10100.15695952309169856No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9880.15354060278673085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.1484122223292793No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT9340.14514870749271924No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC8410.13069599893081038No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8090.12572302394176646No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7950.12354734738405976No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG7520.116864912242532No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA7190.11173653178508046No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7160.1112703153798576No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA7060.10971626069578137No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC6980.10847301694852038No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG6940.1078513950748899No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC6940.1078513950748899No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG6800.10567571851718319No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA6690.10396625836469933No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGA200.002068863371.29162
GGTATCA19500.054.13381
GTATCAA36500.039.0821571
AACCGAT500.001614979638.0014657
CATTCCG2050.032.4402739
GAGTACC1950.031.6999721
GCCTATG753.0762525E-431.6678857
ATCAACG45800.030.7166123
GTACCAC1551.3460522E-930.6630543
TCAACGC46150.030.5890274
ACTGAGT1102.730154E-630.2284378
CAACGCA47350.029.7134195
AACGCAG48500.029.088756
TATCAAC48550.028.9767422
CATATAG2300.028.9299243
TAGACGC700.0083618327.1608224
AGTACCA1754.9221853E-927.1587052
GTGGTAC908.888504E-426.4166411
GGCATTC2355.456968E-1226.2776077
CGCAGAG53150.025.739478
Produced by FastQC (version 0.11.5)