FastQCFastQC Report
Thu 26 May 2016
SRR937173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643478
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57060.8867436027338931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51800.8050003263514837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36830.5723583401452731No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21990.3417366250283615No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11980.18617575115233156No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11640.18089196522647238No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.16395276917004153No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT10490.1630203363595958No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA8710.13535816298303904No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.13458113564100094No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8410.13069599893081038No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG8270.12852032237310368No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC7590.11795275052138535No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT7420.11531085755845576No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC7200.11189193725348806No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC7150.11111490991144997No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT6940.1078513950748899No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG6940.1078513950748899No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6880.10691896226444415No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA6720.1044324747699222No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA6600.10256760914903074No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT6510.10116895993336214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.048.189061
GACTGTA608.287521E-539.5826537
CATTCCG1850.038.512859
GTATCAA43950.037.306341
TAATGCG600.00395000331.6661224
TCAACGC54600.030.0132224
ATCAACG54950.029.8220543
CAACGCA55700.029.4205025
TCACCGC650.00584267629.2302678
GCGGTGA650.00584267629.2302678
CACCGCA650.00584267629.2302679
GGCATTC2450.029.0811337
TAGGCAT2450.029.0811335
AACGCAG56400.029.0553536
TATCAAC57350.028.6568762
ACAGTAC856.404734E-427.9406978
GGATGCC1205.3775657E-627.7078578
GTGGTAC1405.772781E-727.1571621
GCGAAAA350.00838945927.14449990-91
CATATAG2500.026.6140171
Produced by FastQC (version 0.11.5)