FastQCFastQC Report
Thu 26 May 2016
SRR937172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677235
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57540.8496312210680192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48050.709502609876926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31100.45922021159567944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17910.26445768455558266No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11110.16404940677903534No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.16109622213854866No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9980.14736391356028555No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT9680.14293413659955553No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.1387996781028742No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC8830.13038310187748714No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG8520.12580566568473275No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8350.12329545874031908No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8210.12122822949197842No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC7640.11281165326659136No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG7500.11074442401825069No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA7450.11000612785812901No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7440.10985846862610468No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA7260.10720060244966666No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT6990.10321380318500963No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC6860.10129423316869329No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG6850.10114657393666897No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGA250.00501767157.007786
GGTATCA21900.052.503521
CATTCCG2550.039.1229869
CACGCCG250.001616890937.99533584-85
GGCATTC2650.037.6466457
GTATCAA39200.036.6048051
TGGTACT1151.0347503E-733.0455362
CGTGCAG300.003949272431.66981742-43
GGTACTC1051.8992123E-631.6686423
TCTTAGA902.4104718E-531.668642
TAGGCAT3250.030.6942215
CGCAGAA1601.899025E-929.691559
ATCAACG49100.028.9274653
TCAACGC49200.028.8750844
CAACGCA49550.028.6647555
CCTTTAG3500.028.508111
AACGCAG50600.028.0720126
TACTCCC1205.3747553E-627.7100645
CTTTAGG3650.027.3304712
TATCAAC52000.027.3142032
Produced by FastQC (version 0.11.5)