##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937172_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 677235 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.972130796547727 33.0 31.0 34.0 30.0 34.0 2 32.11383788492916 33.0 31.0 34.0 30.0 34.0 3 32.112348003278036 34.0 31.0 34.0 30.0 34.0 4 35.74981210362725 37.0 35.0 37.0 35.0 37.0 5 35.77988142963668 37.0 35.0 37.0 35.0 37.0 6 35.82724460490081 37.0 35.0 37.0 35.0 37.0 7 35.74335939518779 37.0 35.0 37.0 35.0 37.0 8 35.76182861192939 37.0 35.0 37.0 35.0 37.0 9 37.4207520284687 39.0 37.0 39.0 35.0 39.0 10-11 37.340267410869195 39.0 37.0 39.0 35.0 39.0 12-13 37.24995533308231 39.0 37.0 39.0 34.0 39.0 14-15 38.45443162270113 40.0 38.0 41.0 34.0 41.0 16-17 38.3873625846272 40.0 38.0 41.0 34.0 41.0 18-19 38.43296418525327 40.0 38.0 41.0 34.0 41.0 20-21 38.38446034242176 40.0 38.0 41.0 34.0 41.0 22-23 38.25278300737558 40.0 38.0 41.0 34.0 41.0 24-25 38.19859280751881 40.0 38.0 41.0 34.0 41.0 26-27 38.19896195559887 40.0 38.0 41.0 34.0 41.0 28-29 38.14231249123273 40.0 38.0 41.0 34.0 41.0 30-31 37.904105664946435 40.0 37.5 41.0 34.0 41.0 32-33 37.66103937333422 40.0 37.0 41.0 33.0 41.0 34-35 37.646980737853184 40.0 37.0 41.0 33.0 41.0 36-37 37.50939334204523 40.0 37.0 41.0 33.0 41.0 38-39 37.401714323683805 40.0 37.0 41.0 33.0 41.0 40-41 37.227054862787654 40.0 36.5 41.0 33.0 41.0 42-43 36.954217516814694 40.0 36.0 41.0 32.0 41.0 44-45 36.66495677275982 40.0 36.0 41.0 31.0 41.0 46-47 36.2642945211042 40.0 36.0 41.0 30.0 41.0 48-49 35.90553279142395 39.5 36.0 41.0 29.0 41.0 50-51 34.93695910577569 38.0 34.5 40.0 27.0 40.5 52-53 35.07588429422579 38.5 35.0 40.0 27.5 40.5 54-55 35.507961785790755 39.0 36.0 40.5 27.5 41.0 56-57 35.368589928163786 39.0 35.0 41.0 26.5 41.0 58-59 35.172625454975005 39.0 35.0 41.0 26.0 41.0 60-61 34.9455270327139 39.0 35.0 40.0 26.0 41.0 62-63 34.67828818652315 38.5 35.0 40.0 25.5 41.0 64-65 34.32433940951073 38.0 34.5 40.0 23.0 41.0 66-67 34.00148692846649 38.0 34.0 40.0 22.5 41.0 68-69 33.54906568620937 37.0 34.0 39.5 21.5 41.0 70-71 33.13566782579164 36.5 33.5 39.0 19.5 40.5 72-73 32.68463236542706 36.0 33.0 39.0 18.0 40.0 74-75 32.175185866058314 35.5 32.5 38.0 13.5 40.0 76-77 31.71673865054228 35.0 32.0 37.0 8.0 39.0 78-79 31.22502085686652 35.0 31.5 37.0 6.0 39.0 80-81 30.714840491114607 35.0 31.0 36.0 2.0 38.5 82-83 30.231466920640543 34.5 31.0 36.0 2.0 37.5 84-85 29.70850221857996 34.0 30.0 35.5 2.0 37.0 86-87 29.298040561991037 34.0 29.5 35.0 2.0 36.5 88-89 28.85777684260264 34.0 29.0 35.0 2.0 36.0 90-91 28.397884781501254 34.0 29.0 35.0 2.0 36.0 92-93 27.954210872149254 34.0 29.0 35.0 2.0 35.0 94-95 27.477431762977403 34.0 27.0 35.0 2.0 35.0 96-97 26.8435498755971 33.5 25.5 35.0 2.0 35.0 98-99 26.055904523540573 33.0 24.5 35.0 2.0 35.0 100-101 23.50438547919112 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09601599453821308 1101 2 0.2725212933463297 1101 3 0.21767658772262166 1101 4 0.13667192918302362 1101 5 0.11407991529288353 1101 6 0.10714397597189418 1101 7 0.12602339115884575 1101 8 0.1872310292926187 1101 9 0.24707087270467554 1101 10-11 0.1786471490108923 1101 12-13 0.2099701063271553 1101 14-15 0.23085284983152832 1101 16-17 0.11763519414787282 1101 18-19 0.4238813964029262 1101 20-21 0.3301141433096646 1101 22-23 0.16934581821766415 1101 24-25 0.38691554235484205 1101 26-27 0.3463966394755076 1101 28-29 0.3016071184861673 1101 30-31 0.2592805768623663 1101 32-33 0.2292330852519413 1101 34-35 0.2989052091525153 1101 36-37 0.43087261084703243 1101 38-39 0.3538503025244708 1101 40-41 0.34040075924870195 1101 42-43 0.24580016401168336 1101 44-45 0.29980018779927065 1101 46-47 0.027258815778864687 1101 48-49 0.0011099127549414334 1101 50-51 0.4275424519927782 1101 52-53 0.3150673675775195 1101 54-55 0.24322263200017602 1101 56-57 0.3835880856951377 1101 58-59 0.23378754557840864 1101 60-61 0.33890169131323233 1101 62-63 0.25474378024109967 1101 64-65 0.42288990064382403 1101 66-67 0.09196414061585045 1101 68-69 0.2393112108254556 1101 70-71 0.08513180132955966 1101 72-73 0.2159727223293757 1101 74-75 0.30034770160326474 1101 76-77 0.4105925501978014 1101 78-79 0.46348672081670017 1101 80-81 0.5555058756125 1101 82-83 0.5806835359891913 1101 84-85 0.6384824497704038 1101 86-87 0.4400442331031087 1101 88-89 0.2155841388029458 1101 90-91 0.36963204053462917 1101 92-93 0.3977752760825801 1101 94-95 0.31384602800807926 1101 96-97 0.28021938510508804 1101 98-99 0.40526512453081764 1101 100-101 0.8295942175895696 1102 1 0.003056622423720512 1102 2 0.14126552233986445 1102 3 0.12539049969553417 1102 4 0.08300926374750617 1102 5 0.12962315936798774 1102 6 0.16134911601498203 1102 7 0.17488073187907105 1102 8 0.08905466733509115 1102 9 -0.05114011529163065 1102 10-11 -0.018036445941405077 1102 12-13 9.250155299938001E-4 1102 14-15 0.08075281905282594 1102 16-17 0.14604778263663576 1102 18-19 0.1514764256426986 1102 20-21 0.14185044678766445 1102 22-23 -0.14129014669461526 1102 24-25 0.034949552819604435 1102 26-27 -0.15090830050602477 1102 28-29 0.009567260068187977 1102 30-31 0.03870309148225459 1102 32-33 -0.013472362578156094 1102 34-35 -0.0048863420940250535 1102 36-37 -0.14503151349892107 1102 38-39 0.02089016094245011 1102 40-41 0.24898609319760823 1102 42-43 0.20529154597536348 1102 44-45 0.27659304714060085 1102 46-47 0.15764124114052436 1102 48-49 0.010653233776793058 1102 50-51 0.22047874008141832 1102 52-53 0.12658437280990853 1102 54-55 0.3362493325234155 1102 56-57 0.20169750397768382 1102 58-59 0.12904185459656503 1102 60-61 0.45468539399097807 1102 62-63 0.4927170027711085 1102 64-65 0.3275644158792872 1102 66-67 0.04482463615293142 1102 68-69 -0.057691713151150736 1102 70-71 -0.30443229531556426 1102 72-73 -0.1657735842264927 1102 74-75 -0.13971462526653866 1102 76-77 -0.025766714191245654 1102 78-79 -0.007034978167606454 1102 80-81 -0.019053604227448062 1102 82-83 -0.03832423563284593 1102 84-85 0.2963502552490134 1102 86-87 0.21807978250292237 1102 88-89 3.679184274467673E-4 1102 90-91 0.015440366173283593 1102 92-93 0.15272872329710552 1102 94-95 0.19776608033187415 1102 96-97 0.28923562086993826 1102 98-99 0.4091971036013007 1102 100-101 0.5207857380574055 1103 1 -0.010131546445389716 1103 2 -0.05616723124383327 1103 3 0.018519028524075054 1103 4 0.04860271242528569 1103 5 0.03530031602148398 1103 6 -0.10758735371845773 1103 7 0.042211915749014395 1103 8 -0.02082907999700012 1103 9 -0.0792269415029736 1103 10-11 0.14444860620579192 1103 12-13 0.19756118100840325 1103 14-15 0.005124252381612848 1103 16-17 0.1389239008873986 1103 18-19 0.09934815414427334 1103 20-21 0.1271656005045756 1103 22-23 0.027030244447175278 1103 24-25 0.18238457696322996 1103 26-27 -0.026315109158375094 1103 28-29 -0.1036069070147434 1103 30-31 -0.2032080388024795 1103 32-33 -0.165439045895603 1103 34-35 -0.14101965423547114 1103 36-37 -0.16723758951727064 1103 38-39 -0.2648427757997496 1103 40-41 -0.16780734639792882 1103 42-43 -0.22822078316500694 1103 44-45 -0.20912513096211427 1103 46-47 -0.17176213139781282 1103 48-49 -0.2511555699408632 1103 50-51 -0.49527175565749815 1103 52-53 -0.503933087795879 1103 54-55 -0.35856470861912726 1103 56-57 -0.27071519299338576 1103 58-59 -0.3738058059698659 1103 60-61 -0.24228000267584093 1103 62-63 -0.22421796839277874 1103 64-65 -0.3304570938916953 1103 66-67 -0.14397620546794343 1103 68-69 -0.2424055469158901 1103 70-71 -0.2940917870128814 1103 72-73 -0.3164384798564228 1103 74-75 -0.29849109621093817 1103 76-77 -0.294063642880527 1103 78-79 -0.3053657381996935 1103 80-81 -0.3147013192873196 1103 82-83 -0.28549360426582027 1103 84-85 -0.2469228325611077 1103 86-87 -0.2868000291919728 1103 88-89 -0.3005588484466024 1103 90-91 -0.3234804366967019 1103 92-93 -0.49640732865330506 1103 94-95 -0.6354139902068567 1103 96-97 -0.42735119777287167 1103 98-99 -0.24769480260943766 1103 100-101 0.32719212287189947 1104 1 -0.04680487342115569 1104 2 -0.15374832807564687 1104 3 -0.10314135626014576 1104 4 -0.05272480827465387 1104 5 -0.0905984472802146 1104 6 -0.10319382027310553 1104 7 -0.082736996652784 1104 8 0.003182091157803768 1104 9 -0.06637557235534786 1104 10-11 -3.090577519770932E-5 1104 12-13 -0.16389492906834136 1104 14-15 -0.17963499258152638 1104 16-17 -0.13442313149078444 1104 18-19 -0.0071108319750337046 1104 20-21 -0.025739580913175075 1104 22-23 -0.2347804514037577 1104 24-25 -0.18596457460422045 1104 26-27 -0.1682213198966096 1104 28-29 -0.1760283077988518 1104 30-31 -0.1468924763847852 1104 32-33 -0.19283524346458591 1104 34-35 -0.20640988780039748 1104 36-37 -0.17896503150446108 1104 38-39 -0.12038407728469736 1104 40-41 -0.05226044419851661 1104 42-43 -0.07794945125454689 1104 44-45 -0.12299144039402421 1104 46-47 -0.09789892506447018 1104 48-49 0.1041435384859426 1104 50-51 0.00856738274070068 1104 52-53 -0.027847760154081413 1104 54-55 0.17696955413006776 1104 56-57 -0.04345485059295129 1104 58-59 -0.08148446119291464 1104 60-61 -0.13811238994254182 1104 62-63 0.24271700277110142 1104 64-65 0.12950347405103457 1104 66-67 0.026126575211108616 1104 68-69 0.17014762202891376 1104 70-71 0.1457062088395631 1104 72-73 0.11885245455467697 1104 74-75 0.1760748484176773 1104 76-77 0.026172343980501722 1104 78-79 0.188255880558156 1104 80-81 0.3195890550523295 1104 82-83 0.15696662309291298 1104 84-85 0.35590704195261225 1104 86-87 0.34411856366635973 1104 88-89 0.19496625637758314 1104 90-91 0.5833074021843672 1104 92-93 0.4068838479508443 1104 94-95 0.6548297922432305 1104 96-97 0.43535750452644706 1104 98-99 0.9507483501386993 1104 100-101 0.9563813059244417 1105 1 -0.05696754455643216 1105 2 0.059283981960874144 1105 3 -0.009809694856258488 1105 4 0.036740516288652714 1105 5 0.023544935869018957 1105 6 0.011962220141988666 1105 7 0.016609810911937473 1105 8 -0.12069078140697087 1105 9 -0.131375629352938 1105 10-11 -0.08613337225111195 1105 12-13 -0.10098668379630027 1105 14-15 -0.24469584701346747 1105 16-17 -0.10518716524855876 1105 18-19 -0.017193706477726778 1105 20-21 -0.12519278398800537 1105 22-23 -0.06454478534886476 1105 24-25 -0.187895843104684 1105 26-27 -0.13816268837086199 1105 28-29 -0.11312196244661976 1105 30-31 0.045760343666209735 1105 32-33 -0.07707692846548042 1105 34-35 0.053680586381595674 1105 36-37 0.20095148601849644 1105 38-39 0.36785921884820993 1105 40-41 0.20733320094965535 1105 42-43 0.04125626826408535 1105 44-45 -0.33037813867534993 1105 46-47 -0.3391849427945175 1105 48-49 -0.11650840000226026 1105 50-51 -0.13452887859706664 1105 52-53 0.026135611747626797 1105 54-55 0.14101927123200397 1105 56-57 0.10380413507785136 1105 58-59 0.13046964434383312 1105 60-61 0.21432178733518015 1105 62-63 0.06739810122875411 1105 64-65 0.10126781559581843 1105 66-67 0.035508379298974546 1105 68-69 -0.07202280837892516 1105 70-71 -0.03790489277195519 1105 72-73 0.3623461791194984 1105 74-75 0.41795744097226617 1105 76-77 0.38146875047214834 1105 78-79 0.5218200541500302 1105 80-81 0.5651166437880839 1105 82-83 0.6007023974432357 1105 84-85 0.38630480916683396 1105 86-87 0.49812036479034916 1105 88-89 0.5969455928495826 1105 90-91 0.7591810803151482 1105 92-93 0.840290708181346 1105 94-95 0.864060362713154 1105 96-97 0.836520825434306 1105 98-99 0.8058481149286223 1105 100-101 0.8466466867253679 1106 1 -0.16994445743918618 1106 2 -0.1305860512864392 1106 3 -0.06652774372747672 1106 4 -0.10984407834992993 1106 5 -0.02057168546041055 1106 6 0.0198322998890319 1106 7 -0.267550055168833 1106 8 -0.21488761477517926 1106 9 -0.19946867531792378 1106 10-11 -0.5649498378064379 1106 12-13 -0.3365223607161525 1106 14-15 -0.2765859448954089 1106 16-17 -0.2885559546280234 1106 18-19 -0.3225051384369948 1106 20-21 -0.40144188305567496 1106 22-23 -0.32263864505917184 1106 24-25 -0.3578349284549489 1106 26-27 -0.47941550741715133 1106 28-29 -0.10077047096769576 1106 30-31 -0.3649543572242635 1106 32-33 -0.4107386661604906 1106 34-35 -0.19399733322036639 1106 36-37 -0.43591175826784934 1106 38-39 -0.5248455543493478 1106 40-41 -0.6207456625968746 1106 42-43 -0.5896753538790875 1106 44-45 -0.7785425617299211 1106 46-47 -0.6949398659537138 1106 48-49 -0.534683401745994 1106 50-51 -0.3874292994196509 1106 52-53 -0.21316944974827123 1106 54-55 -0.40436446781149726 1106 56-57 -0.4943073945308569 1106 58-59 -0.6782769553632164 1106 60-61 -0.6626166702686902 1106 62-63 -0.7917251463125723 1106 64-65 -0.547519703875949 1106 66-67 -0.5306229591958314 1106 68-69 -0.5952697684589126 1106 70-71 -0.3815466355819339 1106 72-73 -0.6940202155237358 1106 74-75 -0.7669545582837358 1106 76-77 -0.4738589563935385 1106 78-79 -0.5452703413301911 1106 80-81 -0.7312290584741312 1106 82-83 -0.7653028105268156 1106 84-85 -0.4326804504179229 1106 86-87 -0.41299249006073424 1106 88-89 -0.10741491957746874 1106 90-91 0.06081612716362983 1106 92-93 0.10334260188295374 1106 94-95 0.16604706378962675 1106 96-97 0.39807484649868385 1106 98-99 0.3282406424215871 1106 100-101 0.5947896036535987 1107 1 -0.12643416772418448 1107 2 -0.1664448633527904 1107 3 0.0011470714191119669 1107 4 -0.1527964682825882 1107 5 -0.08913442561269136 1107 6 -0.240655823131803 1107 7 -0.08000929312488125 1107 8 -0.15221032443942306 1107 9 -0.2165533739341754 1107 10-11 -0.12732951611042154 1107 12-13 -0.05363868674964323 1107 14-15 0.11636899880385698 1107 16-17 0.04877991675643756 1107 18-19 0.18582603401689823 1107 20-21 -0.12716931365216055 1107 22-23 -0.0011832731128862406 1107 24-25 -0.0427649552498508 1107 26-27 -0.32046416256436316 1107 28-29 -0.21031723632761867 1107 30-31 -0.15222363423328744 1107 32-33 -0.15899130046896914 1107 34-35 -0.1664372804163321 1107 36-37 -0.07413241156063322 1107 38-39 0.009898440370250228 1107 40-41 -0.2374842276157736 1107 42-43 -0.5294103907711971 1107 44-45 -0.5849316928992323 1107 46-47 -0.15816268012594037 1107 48-49 -0.24950919557452522 1107 50-51 -0.02760358416855979 1107 52-53 -0.18661224144735655 1107 54-55 0.014665415186705388 1107 56-57 -0.0668117752228028 1107 58-59 -0.029337357836830336 1107 60-61 -0.040511266498540976 1107 62-63 0.045900479360241775 1107 64-65 0.06219796740322181 1107 66-67 0.12258633965772248 1107 68-69 0.15546984163986366 1107 70-71 0.17224256473551947 1107 72-73 -0.03602947980321858 1107 74-75 0.048417938042767616 1107 76-77 0.035802139133050304 1107 78-79 0.28630776856820717 1107 80-81 0.2497561846837435 1107 82-83 0.34384320991903294 1107 84-85 0.18334993547585654 1107 86-87 0.3679012103863748 1107 88-89 0.3615411006326461 1107 90-91 0.45880199108323083 1107 92-93 0.4696971196925297 1107 94-95 0.4795690615423105 1107 96-97 0.4909219426696083 1107 98-99 0.4342366899766539 1107 100-101 0.6977859576606527 1108 1 -0.007276466860510311 1108 2 0.07311166573506966 1108 3 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0.21912592815673548 2316 8 0.13774195247795973 2316 9 0.28846445973779566 2316 10-11 -0.18670990995081382 2316 12-13 0.19500945328511676 2316 14-15 0.23818782751533973 2316 16-17 0.02838513542106824 2316 18-19 0.05608864329041552 2316 20-21 0.15286050224788994 2316 22-23 0.33261660750589783 2316 24-25 0.3123941657985796 2316 26-27 0.44087778817176826 2316 28-29 0.4079877856226446 2316 30-31 0.13080835464014484 2316 32-33 0.09618164057258838 2316 34-35 -0.11033104877560618 2316 36-37 -0.06142536895094963 2316 38-39 -0.07626186566908899 2316 40-41 0.12828982431799574 2316 42-43 0.14861611421135734 2316 44-45 -0.11286131090874107 2316 46-47 -0.4104995821539461 2316 48-49 -0.34497741400098647 2316 50-51 -0.5518444401848086 2316 52-53 -0.49103581541519503 2316 54-55 -0.6313266397874671 2316 56-57 -0.5146413011970381 2316 58-59 -0.5889206409379213 2316 60-61 -0.6010251938171436 2316 62-63 -0.5869228864255689 2316 64-65 -0.5831618963963408 2316 66-67 -0.5131348140470635 2316 68-69 -0.49565766396899846 2316 70-71 -0.4449700896734967 2316 72-73 -0.5648456356692151 2316 74-75 -0.4913277740838353 2316 76-77 -0.5844465031261379 2316 78-79 -0.5137683884151549 2316 80-81 -0.5438701521065532 2316 82-83 -0.23365901745220086 2316 84-85 -0.4045378697034039 2316 86-87 -0.4565642940286736 2316 88-89 -0.5985073575559916 2316 90-91 -0.534260078328419 2316 92-93 -0.2833748883524727 2316 94-95 -0.2913322078387601 2316 96-97 0.004953646321908423 2316 98-99 -0.17687605470338497 2316 100-101 0.11981767307465319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2604.0 3 483.0 4 241.0 5 274.0 6 331.0 7 372.0 8 516.0 9 590.0 10 815.0 11 1097.0 12 1829.0 13 2685.0 14 3127.0 15 4627.0 16 6529.0 17 9519.0 18 7554.0 19 3958.0 20 3531.0 21 3317.0 22 3615.0 23 3980.0 24 4483.0 25 5133.0 26 5964.0 27 7059.0 28 8556.0 29 10525.0 30 12875.0 31 16370.0 32 21428.0 33 28489.0 34 39625.0 35 58357.0 36 87303.0 37 147003.0 38 143497.0 39 18974.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.38776549520861 25.078203300453154 16.708636634822785 20.825394569515442 2 14.075873082120113 23.367091867316088 41.476525164981695 21.080509885582103 3 15.414542467942788 29.497961509149164 33.746013658998585 21.341482363909464 4 10.528333160675437 20.79042784626541 42.66016486362493 26.021074129434226 5 10.074528572739354 40.240989531073794 37.84988915428717 11.83459274189968 6 24.392139447096703 43.401354502053955 19.322748973020985 12.883757077828356 7 23.014974204102177 36.28257796134686 25.20758850916735 15.494859325383608 8 22.51991953735459 37.02702662697808 22.59777706400155 17.855276771665782 9 24.284889658540916 17.698888444858873 25.008806595325844 33.00741530127437 10-11 20.78669980416508 29.547314930081157 31.12872260272452 18.53726266302924 12-13 21.955160229794203 29.085160333411785 30.28734636842565 18.672333068368363 14-15 20.37601780186618 30.403553861079434 28.774996873425152 20.44543146362924 16-17 17.300488716879496 32.96244732952455 30.549948967092472 19.187114986503477 18-19 17.04693884045294 31.156416672031995 34.178242822117575 17.61840166539749 20-21 17.47924344003907 30.622030812946765 34.40601459249064 17.492711154523523 22-23 18.244138222945104 30.141144398845032 33.752780486938555 17.861936891271306 24-25 17.824733846549996 31.12522900887622 33.760643918204984 17.289393226368798 26-27 17.362839847854723 30.911317655068306 34.18739917415306 17.538443322923914 28-29 17.228812580858126 29.917284173567054 34.95032313645151 17.90358010912331 30-31 17.93944911349683 30.04733197887639 34.392770308527524 17.62044859909925 32-33 17.47692603749565 30.83014454995596 33.96938767957723 17.723541732971157 34-35 17.922541648871167 30.462465867799853 34.03693716566358 17.5780553176654 36-37 18.492323539074917 29.853396911656322 33.20783422277868 18.44644532649008 38-39 17.234321537241158 31.40672543006997 33.26227164557262 18.096681387116252 40-41 17.347793465859063 30.509676015881777 34.42129626204045 17.72123425621871 42-43 17.62513800625727 30.441886416040397 33.534854534745016 18.398121042957317 44-45 17.65194376104662 30.84386555467725 33.13475322183834 18.36943746243779 46-47 18.24468010300419 30.818430318583168 32.515379200644254 18.421510377768385 48-49 17.90062274557294 31.02772635317439 32.28654142668176 18.785109474570913 50-51 17.767353791126798 30.96131297818414 32.13108759504465 19.14024563564441 52-53 17.956385637793783 31.619968370924944 32.21708562357847 18.206560367702807 54-55 18.568113431708916 31.22962074695861 31.60276695202247 18.59949886931 56-57 18.231563026752166 31.80188272970649 31.405417324605867 18.56113691893548 58-59 17.862287184184027 30.75452560744013 32.27602043949199 19.107166768883854 60-61 18.652021036325316 31.428133902765065 31.387742027598154 18.53210303331146 62-63 18.097444191094546 31.177820556261683 32.33572494980269 18.389010302841086 64-65 18.295810730135386 30.74000397998762 31.942507673312555 19.021677616564443 66-67 17.979716678589476 31.24710098235548 31.3587339680163 19.41444837103875 68-69 18.030703308621444 30.49091114890683 32.121881082568684 19.35650445990304 70-71 18.256081402497443 30.79589229290754 32.30983433490937 18.63819196968565 72-73 18.49719236204103 30.155289382763545 32.07772607219218 19.269792183003247 74-75 17.77964062641039 31.23565561338114 31.498164362439645 19.486539397768823 76-77 17.71238792672206 30.98025083102206 32.16876777854409 19.13859346371179 78-79 17.89913758976503 30.547525056513585 31.692077739012685 19.8612596147087 80-81 17.861624424372284 30.877321747777398 31.257398813822167 20.00365501402815 82-83 18.10050228205775 30.888385957409977 31.4568843123604 19.554227448171876 84-85 17.806210474293678 30.276654050006957 31.46963863479653 20.447496840902836 86-87 17.591861589606346 30.6854445782036 31.712068734614512 20.010625097575538 88-89 18.58220467838421 29.837333106430837 31.617679157402506 19.962783057782445 90-91 18.0293611192715 29.709578142031212 32.11260497610219 20.1484557625951 92-93 18.412723075557132 29.811107165053713 31.912604693835274 19.86356506555388 94-95 18.292656760854012 28.918753149052566 32.112974503494755 20.675615586598667 96-97 18.761085520839114 28.820837264369093 31.41794772184916 21.000129492942634 98-99 18.260851547514743 29.09138269909304 31.50101233749319 21.146753415899028 100-101 18.49179018076768 28.682101151483092 32.05857657011175 20.76753209763748 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1586.0 1 1005.5 2 446.5 3 499.0 4 463.0 5 477.5 6 745.5 7 1377.0 8 2116.0 9 2307.5 10 1908.0 11 1515.0 12 1456.0 13 1426.0 14 1404.5 15 1393.5 16 1373.0 17 1442.0 18 1490.0 19 1608.0 20 1896.5 21 2176.5 22 2432.5 23 2866.5 24 3167.0 25 3961.0 26 6300.0 27 8816.0 28 9543.0 29 10669.5 30 13939.0 31 17219.0 32 21330.5 33 27357.5 34 33447.5 35 37583.5 36 42736.5 37 47379.0 38 45137.5 39 40200.5 40 35939.0 41 31657.5 42 27869.0 43 26021.0 44 24695.0 45 23319.5 46 20453.5 47 15822.0 48 13211.0 49 11260.0 50 8940.0 51 6356.0 52 4563.0 53 3984.0 54 3548.0 55 3085.0 56 2583.0 57 2102.0 58 1814.5 59 1478.0 60 1184.0 61 995.0 62 758.0 63 519.5 64 342.5 65 241.0 66 178.5 67 163.5 68 136.5 69 99.0 70 90.0 71 80.0 72 59.0 73 58.0 74 57.5 75 48.5 76 34.0 77 19.0 78 16.5 79 17.5 80 11.5 81 4.5 82 2.0 83 2.5 84 2.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2586989745066336 2 0.22946244656581544 3 0.22001225571625802 4 0.2251803288371097 5 0.22458969190901237 6 0.25249728676161154 7 0.2569270637223416 8 0.24245645898395685 9 0.23699306739905646 10-11 0.24600028055254083 12-13 0.24718155440873552 14-15 0.2326371200543386 16-17 0.24961793173713703 18-19 0.23632860085494695 20-21 0.22813351347759642 22-23 0.22798585424557205 24-25 0.22104587034042833 26-27 0.2310866981180831 28-29 0.24644325824861388 30-31 0.24917495404106405 32-33 0.24954410212112485 34-35 0.2564102564102564 36-37 0.22636160269330438 38-39 0.22606628422925573 40-41 0.2203075741803067 42-43 0.22754287654949906 44-45 0.2493964428891005 46-47 0.25522898255406173 48-49 0.2596587595147917 50-51 0.26032322605890124 52-53 0.28158615547040544 54-55 0.2602493964428891 56-57 0.22355607728484203 58-59 0.20472952520173943 60-61 0.20029974824100938 62-63 0.2070182432981166 64-65 0.1998567705449364 66-67 0.2007427259370824 68-69 0.20369591057756908 70-71 0.2042865475056664 72-73 0.20672292483406796 74-75 0.21381056797123596 76-77 0.23049606118998575 78-79 0.2233345884368055 80-81 0.21425354566730898 82-83 0.20960227985854246 84-85 0.20805185792228692 86-87 0.2180188560839295 88-89 0.2166899229957105 90-91 0.22820734309360857 92-93 0.2148441825954063 94-95 0.21211248680295614 96-97 0.2248111807570489 98-99 0.23197265351022908 100-101 0.20192399979327708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 677235.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.37889431087748 #Duplication Level Percentage of deduplicated Percentage of total 1 73.38130434022044 17.155737585643486 2 10.848159498643426 5.0723594877265255 3 4.202374288683528 2.9474059304964326 4 2.1991177356478566 2.056517644755498 5 1.315769203568715 1.538061457387021 6 0.916818714102861 1.286052469154723 7 0.7219968431180535 1.181564152163091 8 0.5287828723291126 0.9889887108467634 9 0.41799740977123967 0.8795085538736122 >10 4.053531058731346 20.243168817931007 >50 0.776990508816684 12.900087653483826 >100 0.6023574628611427 26.422347479735357 >500 0.031003692941382343 4.715550290891299 >1k 0.0031636421368757494 1.761489831982357 >5k 6.327284273751499E-4 0.8511599339289959 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5754 0.8496312210680192 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4805 0.709502609876926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3110 0.45922021159567944 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1791 0.26445768455558266 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1111 0.16404940677903534 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1091 0.16109622213854866 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 998 0.14736391356028555 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 968 0.14293413659955553 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 940 0.1387996781028742 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 883 0.13038310187748714 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 852 0.12580566568473275 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 835 0.12329545874031908 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 821 0.12122822949197842 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 764 0.11281165326659136 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 750 0.11074442401825069 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 745 0.11000612785812901 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 744 0.10985846862610468 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 726 0.10720060244966666 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 699 0.10321380318500963 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 686 0.10129423316869329 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 685 0.10114657393666897 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.4765923202433425E-4 0.0 24-25 0.0 0.0 0.0 2.953184640486685E-4 0.0 26-27 0.0 0.0 0.0 8.859553921460055E-4 0.0 28-29 0.0 0.0 0.0 0.0028055254084623504 0.0 30-31 0.0 0.0 0.0 0.007604450449253213 0.0 32-33 0.0 0.0 0.0 0.01742378937887144 0.0 34-35 0.0 0.0 0.0 0.03182056450124403 0.0 36-37 0.0 0.0 0.0 0.04614351000760445 0.0 38-39 0.0 0.0 0.0 0.06245985514629338 0.0 40-41 0.0 0.0 0.0 0.07729960796473898 0.0 42-43 0.0 0.0 0.0 0.09368978271944008 0.0 44-45 0.0 0.0 0.0 0.11724143022732139 0.0 46-47 0.0 0.0 0.0 0.15592814901769697 0.0 48-49 0.0 0.0 0.0 0.1996352816968999 0.0 50-51 0.0 0.0 0.0 0.24452368823229748 0.0 52-53 0.0 0.0 0.0 0.3205681927248296 0.0 54-55 0.0 0.0 0.0 0.44438045877723387 0.0 56-57 0.0 0.0 0.0 0.6219406852864958 0.0 58-59 0.0 0.0 0.0 0.8158172569344466 0.0 60-61 0.0 0.0 0.0 1.0127208428388963 0.0 62-63 0.0 0.0 0.0 1.235169475883556 0.0 64-65 0.0 0.0 0.0 1.5126950024732921 0.0 66-67 0.0 0.0 0.0 1.8727620397646312 0.0 68-69 0.0 0.0 0.0 2.2983897760747745 0.0 70-71 0.0 0.0 0.0 2.748528944900958 0.0 72-73 0.0 0.0 0.0 3.1793247543319527 0.0 74-75 0.0 0.0 0.0 3.6762718997098496 0.0 76-77 0.0 0.0 0.0 4.241511439899002 0.0 78-79 0.0 0.0 0.0 4.826315828331377 0.0 80-81 0.0 0.0 0.0 5.445155669745362 0.0 82-83 0.0 0.0 0.0 6.085701418266924 0.0 84-85 0.0 0.0 0.0 6.791143399263181 0.0 86-87 0.0 0.0 0.0 7.576985832096687 0.0 88-89 0.0 0.0 0.0 8.478076295525186 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTGA 25 0.005017671 57.00778 6 GGTATCA 2190 0.0 52.50352 1 CATTCCG 255 0.0 39.122986 9 CACGCCG 25 0.0016168909 37.995335 84-85 GGCATTC 265 0.0 37.646645 7 GTATCAA 3920 0.0 36.604805 1 TGGTACT 115 1.0347503E-7 33.045536 2 CGTGCAG 30 0.0039492724 31.669817 42-43 GGTACTC 105 1.8992123E-6 31.668642 3 TCTTAGA 90 2.4104718E-5 31.66864 2 TAGGCAT 325 0.0 30.694221 5 CGCAGAA 160 1.899025E-9 29.69155 9 ATCAACG 4910 0.0 28.927465 3 TCAACGC 4920 0.0 28.875084 4 CAACGCA 4955 0.0 28.664755 5 CCTTTAG 350 0.0 28.50811 1 AACGCAG 5060 0.0 28.072012 6 TACTCCC 120 5.3747553E-6 27.710064 5 CTTTAGG 365 0.0 27.330471 2 TATCAAC 5200 0.0 27.314203 2 >>END_MODULE