FastQCFastQC Report
Thu 26 May 2016
SRR937172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677235
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60670.8958485606916358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55140.814193005382179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38000.5611050816924701No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20860.30801715800276125No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.18176851462195545No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11720.17305661993251975No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.16759322834761936No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT10840.1600626075143783No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG8580.12669162107687876No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8560.1263963026128301No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC8420.12432907336448944No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.12196652565210009No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8130.12004695563578374No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA8130.12004695563578374No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC7950.11738908945934572No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA7610.11236867557051836No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA7370.10882485400193433No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG7240.10690528398561798No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC7130.10528103243335031No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7040.10395209934513132No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT7000.10336146241703398No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT6910.10203252932881494No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG6820.10070359624059595No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCCG352.7875838E-454.2838975
GGTATCA23050.053.41471
GTATCAA41300.037.9835781
GACATAT2900.032.7841531
TCAACGC53100.029.4293374
ATCAACG53300.029.3189093
CAACGCA53800.029.0464275
CGCAGAA1004.94878E-528.5011529
AACGCAG55400.028.2932786
CCTGTAC856.4056914E-427.9402433
TATCAAC56100.027.8555762
CTGTACT1205.3787917E-627.7074094
CATATAG3300.027.347573
GCAGCTC700.00838752627.1439538
GGGATGC1457.8767516E-726.2079567
CATTCCG1658.396273E-825.9101379
GGTTACT1109.468393E-525.9101378
CCCTGGC1701.1209704E-725.1480757
ATGGCGG851.1234988E-725.1462230-31
ACGCAGA63300.024.613957
Produced by FastQC (version 0.11.5)