##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937172_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 677235 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04276654337121 31.0 31.0 34.0 30.0 34.0 2 32.26858476009066 33.0 31.0 34.0 30.0 34.0 3 32.2860203622081 34.0 31.0 34.0 30.0 34.0 4 36.0082497212932 37.0 35.0 37.0 35.0 37.0 5 35.92832916196003 37.0 35.0 37.0 35.0 37.0 6 35.971636138120445 37.0 35.0 37.0 35.0 37.0 7 35.906639497367976 37.0 35.0 37.0 35.0 37.0 8 35.89968622413195 37.0 35.0 37.0 35.0 37.0 9 37.56293753276189 39.0 37.0 39.0 35.0 39.0 10-11 37.49428411112834 39.0 37.0 39.0 35.0 39.0 12-13 37.42674773158505 39.0 37.0 39.0 34.0 39.0 14-15 38.54736834333724 40.0 38.0 41.0 34.0 41.0 16-17 38.50757491860285 40.0 38.0 41.0 34.0 41.0 18-19 38.58357881680658 40.0 38.0 41.0 34.0 41.0 20-21 38.54533433741611 40.0 38.0 41.0 34.0 41.0 22-23 38.397829409289244 40.0 38.0 41.0 34.0 41.0 24-25 38.32215257628445 40.0 38.0 41.0 34.0 41.0 26-27 38.23038310187749 40.0 38.0 41.0 34.0 41.0 28-29 38.12349332211123 40.0 37.0 41.0 33.0 41.0 30-31 38.08601224095034 40.0 37.0 41.0 34.0 41.0 32-33 37.95094095845607 40.0 37.0 41.0 33.0 41.0 34-35 37.81198254667878 40.0 37.0 41.0 33.0 41.0 36-37 37.59876113904332 40.0 36.0 41.0 33.0 41.0 38-39 37.462381595753314 40.0 36.0 41.0 33.0 41.0 40-41 37.29281268688122 40.0 36.0 41.0 33.0 41.0 42-43 37.03653901526058 40.0 36.0 41.0 32.0 41.0 44-45 36.671310549513834 40.0 36.0 41.0 31.0 41.0 46-47 36.235900389082076 39.0 35.5 41.0 30.0 41.0 48-49 35.841294380827925 39.0 35.0 41.0 29.0 41.0 50-51 35.614742297725314 39.0 35.0 41.0 27.5 41.0 52-53 35.431498667375436 39.0 35.0 40.5 27.0 41.0 54-55 35.22980944576108 39.0 35.0 40.0 26.5 41.0 56-57 35.00226287773077 39.0 35.0 40.0 26.0 41.0 58-59 34.78883770035512 38.0 35.0 40.0 26.0 41.0 60-61 34.51272970239282 38.0 34.0 40.0 24.5 41.0 62-63 34.172749488729906 38.0 34.0 40.0 23.0 41.0 64-65 33.81036346319963 37.5 34.0 40.0 22.0 41.0 66-67 33.408586384342215 37.0 33.0 39.5 21.5 41.0 68-69 32.957492598581 36.5 33.0 39.0 20.0 40.0 70-71 32.59064800253974 36.0 32.0 39.0 18.5 40.0 72-73 32.10599644141251 35.5 32.0 38.5 16.0 40.0 74-75 31.58853130744867 35.0 31.0 37.5 12.0 39.5 76-77 30.24714611619305 34.0 29.5 36.0 8.0 38.5 78-79 30.824610364201497 34.5 31.0 36.0 7.0 39.0 80-81 30.593579776591582 34.5 31.0 36.0 4.0 38.5 82-83 30.224691576779108 34.0 31.0 36.0 2.0 37.0 84-85 29.802165422637636 34.0 30.0 35.0 2.0 37.0 86-87 29.384845733017343 34.0 30.0 35.0 2.0 36.0 88-89 28.876843340937782 34.0 29.0 35.0 2.0 36.0 90-91 28.373499597628594 34.0 29.0 35.0 2.0 35.5 92-93 28.03095749629006 34.0 29.0 35.0 2.0 35.0 94-95 27.570947307803053 33.0 27.0 35.0 2.0 35.0 96-97 27.0808382614602 33.0 26.5 35.0 2.0 35.0 98-99 26.35383581769991 33.0 25.0 35.0 2.0 35.0 100-101 23.88931094819376 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13924853372098767 1101 2 -0.1106702492159144 1101 3 -0.13751542254640015 1101 4 0.025976529335594023 1101 5 0.01996343157398428 1101 6 0.008149192133622307 1101 7 -0.024712994271524735 1101 8 0.06089340970062551 1101 9 0.08520152223782418 1101 10-11 0.10673883947358576 1101 12-13 0.002850988709695912 1101 14-15 -0.13717930782004117 1101 16-17 -0.00391019723046071 1101 18-19 0.05726010953642913 1101 20-21 -0.043310000056898446 1101 22-23 -0.059642371543581874 1101 24-25 0.0839825091369022 1101 26-27 0.0271679557905955 1101 28-29 0.003221641914457507 1101 30-31 0.12237382464630997 1101 32-33 0.3107125413543841 1101 34-35 0.2095831853604011 1101 36-37 0.3976219287079914 1101 38-39 0.2060477974857875 1101 40-41 0.022311347246521507 1101 42-43 0.1860124604389597 1101 44-45 0.22704783740528 1101 46-47 0.15088597485297583 1101 48-49 0.2099052799298562 1101 50-51 0.21722481443808306 1101 52-53 0.1670985840762782 1101 54-55 0.22550325263659232 1101 56-57 0.32241018374216424 1101 58-59 0.2879501183719597 1101 60-61 0.1563022135204548 1101 62-63 0.17434050167118897 1101 64-65 0.18520301408821638 1101 66-67 0.06657009873222819 1101 68-69 0.15138257370332298 1101 70-71 0.19817107168185544 1101 72-73 0.34474318827360406 1101 74-75 0.09955184269207606 1101 76-77 0.25103640858339915 1101 78-79 -0.006491808691635015 1101 80-81 0.15155868642450088 1101 82-83 0.2695120985809645 1101 84-85 0.0872465139773766 1101 86-87 0.19652156908752616 1101 88-89 -0.14572239732299508 1101 90-91 0.04771618303346514 1101 92-93 -0.07089608571179085 1101 94-95 0.24269753526247229 1101 96-97 0.062070177565104245 1101 98-99 -0.1018351225161247 1101 100-101 0.5166039640256272 1102 1 -0.11261726256063298 1102 2 -0.10968533077947029 1102 3 -0.05620578881294591 1102 4 -0.05724523120611025 1102 5 -0.11979495562207632 1102 6 -0.13305194667832154 1102 7 -0.16840720787570973 1102 8 -0.16199440193372538 1102 9 -0.15066335926417906 1102 10-11 -0.07701000632511779 1102 12-13 -0.1901526277876897 1102 14-15 -0.21311421086712556 1102 16-17 -0.07799260720276635 1102 18-19 -0.360739659623313 1102 20-21 -0.09668603883805815 1102 22-23 -0.03362090339954449 1102 24-25 -0.1927834188409392 1102 26-27 -0.164702619771127 1102 28-29 -0.040753427337619996 1102 30-31 0.16338452024495353 1102 32-33 0.08977340623589214 1102 34-35 -0.04672837204186919 1102 36-37 -0.11065177089188438 1102 38-39 0.10028063835991219 1102 40-41 0.15780688433485324 1102 42-43 -0.02933841675402249 1102 44-45 -0.04966999885203904 1102 46-47 -0.035817626255052915 1102 48-49 0.19861719128719812 1102 50-51 0.2190080554353102 1102 52-53 0.1016784548057359 1102 54-55 0.16668323109150407 1102 56-57 -0.0968111151190243 1102 58-59 -0.011472780981691244 1102 60-61 -0.029037506393358115 1102 62-63 -0.2559822899570108 1102 64-65 -0.07791255993456758 1102 66-67 -0.23179670643859396 1102 68-69 -0.1002390791129244 1102 70-71 0.06934489439653646 1102 72-73 0.27018947944011273 1102 74-75 0.5756637232707114 1102 76-77 0.32520538365264784 1102 78-79 0.10763369146225443 1102 80-81 0.14054760609208827 1102 82-83 0.29176702317314707 1102 84-85 -0.006551577743149295 1102 86-87 -0.04325605479674621 1102 88-89 0.2435896987065469 1102 90-91 0.2578250626887417 1102 92-93 0.5089227046852507 1102 94-95 0.6421108162720124 1102 96-97 0.5748683462323854 1102 98-99 0.6468914087242581 1102 100-101 1.2053774327852445 1103 1 -0.021261284176361528 1103 2 0.039967273552754534 1103 3 0.05952465031334242 1103 4 -0.018445146438537563 1103 5 -0.021612160411443426 1103 6 -0.11300290258404999 1103 7 -0.0759197337979387 1103 8 -0.03685249193871698 1103 9 -0.037430536049974705 1103 10-11 -0.07563820970673163 1103 12-13 -0.14823052313001028 1103 14-15 -0.26347712202768747 1103 16-17 -0.25107549777691673 1103 18-19 -0.09543797789526565 1103 20-21 -0.24785234978367754 1103 22-23 -0.1686246119807393 1103 24-25 -0.04709672583577884 1103 26-27 -0.13769998592161414 1103 28-29 -0.3690461176483879 1103 30-31 -0.21347544675624164 1103 32-33 -0.2454463839643708 1103 34-35 -0.0033152663700164453 1103 36-37 -0.23672461045412518 1103 38-39 -0.4642846251007313 1103 40-41 -0.26735167643289515 1103 42-43 -0.3411733319107739 1103 44-45 -0.4201402318114802 1103 46-47 -0.3017096535623267 1103 48-49 -0.6403679441138621 1103 50-51 -0.5550087921192954 1103 52-53 -0.4229287383280962 1103 54-55 -0.33980867814665316 1103 56-57 -0.47344591826147564 1103 58-59 -0.49907173955153894 1103 60-61 -0.36267547318264093 1103 62-63 -0.377788369002765 1103 64-65 -0.3814864212487592 1103 66-67 -0.20395585755192513 1103 68-69 -0.4272034918704364 1103 70-71 -0.11750515782633641 1103 72-73 -0.23239706673550486 1103 74-75 -0.022693148200456648 1103 76-77 0.05062657251782454 1103 78-79 -0.22453597991203722 1103 80-81 -0.40267628625309726 1103 82-83 -0.3993289761002714 1103 84-85 -0.5286460178987618 1103 86-87 -0.5872443689816897 1103 88-89 -0.3629355120770974 1103 90-91 -0.183579354307156 1103 92-93 -0.2740723152199891 1103 94-95 -0.08959982357177054 1103 96-97 0.4294768260168347 1103 98-99 0.5989617809447125 1103 100-101 1.3004382081057706 1104 1 -0.010124187768390414 1104 2 0.006658159525237295 1104 3 0.021356538056856778 1104 4 -0.1832840123695476 1104 5 -0.12395008027581156 1104 6 -0.0748802015259642 1104 7 -0.10608033806961714 1104 8 -0.0525761193852432 1104 9 -0.21576031217276892 1104 10-11 -0.17257787336112784 1104 12-13 -0.047493348009297165 1104 14-15 -0.1632527150222458 1104 16-17 -0.37162141606703614 1104 18-19 -0.16267871779506038 1104 20-21 -0.14793257623417588 1104 22-23 -0.3341749200200397 1104 24-25 -0.2675756626082517 1104 26-27 -0.2173341987184898 1104 28-29 -0.4001717098860951 1104 30-31 -0.12539664318995136 1104 32-33 0.010826037814837264 1104 34-35 -0.019027541016939153 1104 36-37 -0.40566562130739925 1104 38-39 -0.19750329515809284 1104 40-41 -0.414215276329962 1104 42-43 -0.3638259513800577 1104 44-45 -0.4153209683811241 1104 46-47 -0.7657345237619779 1104 48-49 -0.4551224209011693 1104 50-51 -0.5607703379164946 1104 52-53 -0.47657639283969644 1104 54-55 -0.590934497440351 1104 56-57 -0.7263125001605744 1104 58-59 -0.7496999277960938 1104 60-61 -0.7049377834016681 1104 62-63 -0.7670626223669785 1104 64-65 -0.592455496222648 1104 66-67 -0.6985557092086765 1104 68-69 -0.6431753672015645 1104 70-71 -0.7409044130826885 1104 72-73 -0.8316110745765073 1104 74-75 -0.5005135620478427 1104 76-77 -0.422717054020481 1104 78-79 -0.6437513500889906 1104 80-81 -0.7583443606669107 1104 82-83 -0.9076789602063506 1104 84-85 -0.7683244309287467 1104 86-87 -0.7115386032731976 1104 88-89 -0.8007316309333383 1104 90-91 -0.7698757683361919 1104 92-93 -0.5838750792482656 1104 94-95 -0.6369750563041627 1104 96-97 -0.2596191883936534 1104 98-99 0.06378891564946798 1104 100-101 0.7870948843087895 1105 1 -0.05828145553168795 1105 2 -0.08639041382497226 1105 3 -0.06800239099633387 1105 4 -0.015707284107477903 1105 5 -0.09879514181421456 1105 6 -0.021703345589287437 1105 7 -0.14935291196700007 1105 8 -0.16909630223352679 1105 9 -0.05584786047823087 1105 10-11 -0.10553825243312787 1105 12-13 -0.11487020470594445 1105 14-15 0.03399696104141725 1105 16-17 -0.18353125347189092 1105 18-19 -0.11877727043613362 1105 20-21 -0.01267728400751622 1105 22-23 -0.1195960752472871 1105 24-25 -0.3141141986820273 1105 26-27 0.06504518487152211 1105 28-29 -0.09777287111709398 1105 30-31 -0.02063886396823733 1105 32-33 0.022878179214465888 1105 34-35 -0.039739516971557975 1105 36-37 -0.1673729272590876 1105 38-39 0.00558483452282843 1105 40-41 0.20609049676641433 1105 42-43 0.33803578005487367 1105 44-45 0.10900085523792313 1105 46-47 0.15212054275421139 1105 48-49 0.07386275592436675 1105 50-51 0.05027522596071776 1105 52-53 0.1663441259144065 1105 54-55 0.4650694391935275 1105 56-57 0.44305490253503166 1105 58-59 0.3293424366161233 1105 60-61 0.31142052627766503 1105 62-63 0.375823697830306 1105 64-65 0.391152945501112 1105 66-67 0.6068393031218093 1105 68-69 0.6626737259667053 1105 70-71 0.7052955572785606 1105 72-73 0.6681656848442472 1105 74-75 0.7866403200583285 1105 76-77 0.7122504003529464 1105 78-79 0.5234035959722867 1105 80-81 0.49377404993616025 1105 82-83 0.5922915910363855 1105 84-85 0.5930764179554266 1105 86-87 0.4769913907610537 1105 88-89 0.8283859703038878 1105 90-91 0.9268571295355201 1105 92-93 0.9785980843842275 1105 94-95 1.2491361498029399 1105 96-97 1.227133620637808 1105 98-99 1.1205414206937512 1105 100-101 1.5239084907745983 1106 1 -0.18213930584904858 1106 2 -0.10539717953606953 1106 3 0.0443584004291111 1106 4 -0.02979089062674234 1106 5 -0.24919852699475342 1106 6 -0.3821285856441534 1106 7 -0.08900207148433026 1106 8 -0.13606986713553226 1106 9 -0.21738793162281667 1106 10-11 -0.2011707650461787 1106 12-13 -0.16156756119614357 1106 14-15 -0.20999004228331586 1106 16-17 -0.05203872830391276 1106 18-19 -0.02992209385340061 1106 20-21 0.06435242932163732 1106 22-23 0.03820367365415933 1106 24-25 -0.11594686939417187 1106 26-27 -0.06014372029227388 1106 28-29 -0.05892525074090571 1106 30-31 -0.04310404729343276 1106 32-33 -0.07967116862678125 1106 34-35 0.06317311003083859 1106 36-37 0.12197456524283012 1106 38-39 -0.018269528314583283 1106 40-41 -0.103347786463452 1106 42-43 -0.40452426272488395 1106 44-45 0.04024228184972145 1106 46-47 -0.15377504209617143 1106 48-49 -0.2874111042509355 1106 50-51 -0.02038111776531082 1106 52-53 0.3505731603474658 1106 54-55 0.2534811245763251 1106 56-57 0.22504671858209235 1106 58-59 0.2895037906883431 1106 60-61 0.3842565449705546 1106 62-63 0.4351573566617688 1106 64-65 0.16163898395638654 1106 66-67 0.1746562569243153 1106 68-69 0.09350827991801935 1106 70-71 0.08528265360275356 1106 72-73 -0.16236603959833928 1106 74-75 0.1150650253662775 1106 76-77 0.18493471366814518 1106 78-79 0.33758775816335174 1106 80-81 0.20127620054879358 1106 82-83 0.14887885940959222 1106 84-85 -0.05846911690774448 1106 86-87 0.2384942621195414 1106 88-89 0.4503228004171156 1106 90-91 0.3673607216398658 1106 92-93 0.587613801293859 1106 94-95 0.4174739006108723 1106 96-97 0.5678022867929755 1106 98-99 0.7018607343576981 1106 100-101 1.7796453952873996 1107 1 0.058089590216575004 1107 2 0.04808419368317374 1107 3 0.06157668068338751 1107 4 0.027427232472668095 1107 5 0.040760062697458466 1107 6 0.06513613387369332 1107 7 -0.08191242248972941 1107 8 0.1428996683932553 1107 9 -0.01125574866312462 1107 10-11 -0.028054855473094165 1107 12-13 0.011090634823126777 1107 14-15 0.10122472555966056 1107 16-17 0.2610324314204391 1107 18-19 0.09988586289757961 1107 20-21 0.13682734347578673 1107 22-23 0.13209093860940158 1107 24-25 0.10750476226624528 1107 26-27 -0.03296378582584936 1107 28-29 0.03955682967523444 1107 30-31 0.15602492184921601 1107 32-33 0.09201856051680579 1107 34-35 -0.120697103621211 1107 36-37 0.11387617843755748 1107 38-39 0.022017122165131298 1107 40-41 -0.018945800025925053 1107 42-43 -0.18221887938486958 1107 44-45 -0.07421066205385074 1107 46-47 -0.09125588884660374 1107 48-49 0.2134866429340292 1107 50-51 0.21215701193460745 1107 52-53 0.013575171929772978 1107 54-55 0.05788715775172193 1107 56-57 0.030809194714194632 1107 58-59 -0.04502391964380337 1107 60-61 0.17668835940208538 1107 62-63 0.15813261262776024 1107 64-65 -0.0644705294135619 1107 66-67 -0.21025825082734428 1107 68-69 0.11325380689758191 1107 70-71 0.05457753899757023 1107 72-73 0.298030933598902 1107 74-75 0.36606590543354045 1107 76-77 0.10079301498117488 1107 78-79 0.3068610347637808 1107 80-81 0.29582155151490497 1107 82-83 0.1575037150235552 1107 84-85 0.28176822623204956 1107 86-87 0.36628474324917093 1107 88-89 0.41159100091529766 1107 90-91 0.5135029625111329 1107 92-93 0.6626719485951149 1107 94-95 0.9468864164245794 1107 96-97 1.1331826408628913 1107 98-99 1.048665186555116 1107 100-101 1.257706575677613 1108 1 -0.011735666630464436 1108 2 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-0.42528103032430664 2316 60-61 -0.3840005231485506 2316 62-63 -0.38571793149770883 2316 64-65 -0.5289061003115947 2316 66-67 -0.2839099928815827 2316 68-69 -0.4287486807011831 2316 70-71 -0.30649330463949553 2316 72-73 -0.646312719642772 2316 74-75 -0.5071459491801313 2316 76-77 -0.8127585174208285 2316 78-79 -0.7860446040874542 2316 80-81 -0.5153017786917786 2316 82-83 -1.1626334851634539 2316 84-85 -0.7981774935396508 2316 86-87 -1.1327566785046734 2316 88-89 -0.9069098532778952 2316 90-91 -0.9650290013686984 2316 92-93 -0.862549538847805 2316 94-95 -0.6166767306524896 2316 96-97 -0.657642942002667 2316 98-99 -0.49971802127111786 2316 100-101 -1.1310998179804805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 0.0 5 11.0 6 35.0 7 92.0 8 185.0 9 382.0 10 716.0 11 1220.0 12 1896.0 13 2598.0 14 3351.0 15 4848.0 16 7092.0 17 9901.0 18 8502.0 19 4415.0 20 3414.0 21 3471.0 22 3648.0 23 4091.0 24 4763.0 25 5477.0 26 6479.0 27 7855.0 28 9944.0 29 12311.0 30 15208.0 31 18871.0 32 24108.0 33 31075.0 34 41588.0 35 58168.0 36 86905.0 37 148881.0 38 133610.0 39 12122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.93521431452894 25.60647457882581 17.430796806449752 20.027514300195495 2 14.54472967286097 24.14021720673031 40.73283276853677 20.58222035187195 3 15.826707125296242 29.474406963609383 33.83995215840883 20.85893375268555 4 11.024238262936795 21.57980612342835 41.98749326304754 25.408462350587314 5 10.085111912419192 40.504794558980315 37.78531834276888 11.624775185831613 6 24.885305691525097 43.61071120069104 18.74770205320162 12.756281054582235 7 23.009885785584032 36.632335895221004 25.014950497242467 15.342827821952499 8 23.208044474960683 37.212636677076645 22.16438902301269 17.414929824949983 9 24.497109570533123 17.751297555501413 24.77736679291531 32.97422608105015 10-11 21.173315889843177 29.732168104253404 30.74075523323162 18.3537607726718 12-13 22.290625754495967 29.08657165155152 30.26755396007475 18.355248633877764 14-15 20.703743899827977 30.364275325404034 28.753017785554498 20.178962989213492 16-17 17.328475344599735 33.03757189158859 30.65700975289227 18.9769430109194 18-19 17.08247506404719 31.047568421596637 34.50131785864582 17.368638655710352 20-21 17.538446772538336 30.33762283402364 34.94326194009465 17.180668453343372 22-23 18.321860160181444 29.96860750820989 34.18296831809483 17.52656401351383 24-25 17.925701920244407 30.779356837359163 34.39953693825524 16.8954043041412 26-27 17.543063064659385 30.691851481062294 34.658282556902456 17.106802897375868 28-29 17.180262219459287 29.709665558704053 35.6253294724454 17.48474274939126 30-31 18.086819390948616 29.669309034476292 34.970213375445844 17.27365819912925 32-33 17.71106041477478 30.5709982502381 34.495854467060916 17.2220868679262 34-35 18.090618470693336 30.1711370499162 34.75056664230289 16.98767783708757 36-37 18.686940279223606 29.589212016508306 33.8039971354109 17.91985056885719 38-39 17.54027048298001 31.080366727551667 33.82284974299834 17.556513046469973 40-41 17.568273937407252 30.346482387945102 34.83960515921357 17.24563851543408 42-43 17.804147948620255 30.224882375353317 34.012277857031044 17.958691818995383 44-45 17.913292958347515 30.537502150289153 33.614870476917524 17.93433441444581 46-47 18.482063094789844 30.798984104483672 32.72674920817737 17.992203592549117 48-49 18.088625071061003 30.867719476991002 32.63689856549056 18.406756886457433 50-51 18.071570429762197 30.710683883733118 32.31817611316604 18.89956957333865 52-53 18.21147755210525 31.463967455905262 32.343352012226184 17.981202979763303 54-55 18.91145614151661 31.055172872045894 31.717498357291046 18.315872629146458 56-57 18.526803842093216 31.487297614565108 31.61886199029879 18.367036553042887 58-59 18.097410795366454 30.626222803015203 32.43150457374471 18.844861827873633 60-61 18.83053888236727 31.382238070979795 31.53949515308571 18.247727893567227 62-63 18.45548443302546 30.988061751090832 32.467090448662574 18.089363367221125 64-65 18.55928887313857 30.575944834510913 32.01864936100468 18.84611693134584 66-67 18.182905490708542 31.222544611545477 31.340598167548933 19.253951730197052 68-69 18.281541857700798 30.3470730248732 32.09690875397757 19.274476363448436 70-71 18.38276226125348 30.783184566657066 32.43918285380998 18.394870318279473 72-73 18.76815285683699 30.187084246974834 32.013924265579895 19.030838630608283 74-75 18.068395756273674 31.149305632461406 31.437905601452965 19.344393009811956 76-77 18.018708424697483 30.867867136223026 32.25638072456385 18.85704371451564 78-79 18.108706726616315 30.561843377852593 31.630527069628712 19.698922825902383 80-81 18.0399846729604 30.8481774038387 31.19569366297789 19.91614426022301 82-83 18.159628960786023 30.88560362534017 31.338032848369764 19.616734565504043 84-85 17.728154321958378 30.52211681790697 31.27569737764006 20.47403148249459 86-87 17.419433431526723 30.839738052522385 31.632003661948954 20.108824854001934 88-89 18.361425502225963 30.02598802483628 31.433032846796166 20.17955362614159 90-91 17.936188687269635 29.873195468292224 31.75532794631887 20.43528789811927 92-93 18.1471240775674 30.185797029757794 31.47201600667437 20.195062886000436 94-95 17.961933449984127 29.32689539081707 31.71225645455418 20.998914704644623 96-97 18.66213352824352 29.244132391267435 30.819656396967076 21.27407768352197 98-99 18.000485799232024 29.519021845461214 30.909456030370574 21.571036324936195 100-101 17.949997784898915 29.510241150671174 31.296794010366675 21.242967054063232 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1307.0 1 856.5 2 417.5 3 488.5 4 437.5 5 422.5 6 707.5 7 1385.5 8 2098.0 9 2345.5 10 2016.5 11 1526.0 12 1389.0 13 1371.5 14 1332.0 15 1319.5 16 1346.5 17 1368.5 18 1481.0 19 1642.5 20 1856.5 21 2179.5 22 2535.0 23 2837.0 24 3193.5 25 4099.0 26 6385.0 27 8891.0 28 9479.0 29 10600.0 30 13710.5 31 16753.0 32 21263.5 33 27409.0 34 33269.5 35 37565.5 36 42828.5 37 47136.0 38 45135.0 39 40572.5 40 36506.0 41 32287.0 42 27829.0 43 25835.0 44 25012.5 45 23573.5 46 20542.5 47 16022.0 48 13304.0 49 11303.5 50 8944.5 51 6394.5 52 4634.0 53 3975.5 54 3475.0 55 2944.5 56 2441.5 57 2047.5 58 1788.0 59 1465.5 60 1087.5 61 858.5 62 648.0 63 463.0 64 332.0 65 235.5 66 164.5 67 133.0 68 114.0 69 96.5 70 89.0 71 84.0 72 71.0 73 56.5 74 45.0 75 31.5 76 26.5 77 23.0 78 15.5 79 12.0 80 9.0 81 4.5 82 2.0 83 0.5 84 0.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07323897908406977 2 0.0 3 0.0 4 0.0 5 0.001328933088219008 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.382961601216713E-5 12-13 0.006570835825082874 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.429776960730027E-4 24-25 5.168073120851699E-4 26-27 2.2148884803650134E-4 28-29 0.002362547712389348 30-31 2.2148884803650134E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.953184640486685E-4 40-41 0.0 42-43 0.011960397793971074 44-45 7.382961601216713E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.382961601216713E-5 82-83 2.953184640486685E-4 84-85 2.2148884803650134E-4 86-87 0.0 88-89 0.0 90-91 0.007530620833241046 92-93 0.0025840365604258494 94-95 0.0 96-97 0.0 98-99 7.382961601216713E-5 100-101 0.009597850081581726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 677235.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.54917559949067 #Duplication Level Percentage of deduplicated Percentage of total 1 71.94212585584611 16.222356289241322 2 11.635099508252768 5.247238038582784 3 4.26941256541734 2.888152009328026 4 2.2777921231558023 2.0544933825670744 5 1.4763876657621755 1.6645662364096716 6 0.9495052115965494 1.2846335849353285 7 0.742408167122429 1.1718484488857766 8 0.5841876541245801 1.0538359996727742 9 0.4656660353033977 0.9450346680697441 >10 4.176084385695354 20.04563582259186 >50 0.8166983236032788 12.971514910932457 >100 0.6272715053678474 26.596305605857463 >500 0.03211734980462332 4.589631882425432 >1k 0.003932736710770203 1.5530818864691476 >5k 0.001310912236923401 1.7116712340311382 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6067 0.8958485606916358 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5514 0.814193005382179 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3800 0.5611050816924701 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2086 0.30801715800276125 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1231 0.18176851462195545 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1172 0.17305661993251975 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1135 0.16759322834761936 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1084 0.1600626075143783 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 858 0.12669162107687876 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 856 0.1263963026128301 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 842 0.12432907336448944 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 826 0.12196652565210009 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 813 0.12004695563578374 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 813 0.12004695563578374 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 795 0.11738908945934572 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 761 0.11236867557051836 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 737 0.10882485400193433 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 724 0.10690528398561798 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 713 0.10528103243335031 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 704 0.10395209934513132 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 700 0.10336146241703398 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 691 0.10203252932881494 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 682 0.10070359624059595 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.4765923202433425E-4 0.0 24-25 0.0 0.0 0.0 2.953184640486685E-4 0.0 26-27 0.0 0.0 0.0 8.859553921460055E-4 0.0 28-29 0.0 0.0 0.0 0.002879355024474518 0.0 30-31 0.0 0.0 0.0 0.007899768913301882 0.0 32-33 0.0 0.0 0.0 0.017645278226907944 0.0 34-35 0.0 0.0 0.0 0.0321158829652927 0.0 36-37 0.0 0.0 0.0 0.04643882847165312 0.0 38-39 0.0 0.0 0.0 0.06297666245837855 0.0 40-41 0.0 0.0 0.0 0.07796407450884849 0.0 42-43 0.0 0.0 0.0 0.09435424926354957 0.0 44-45 0.0 0.0 0.0 0.11761057830738222 0.0 46-47 0.0 0.0 0.0 0.15718325248990378 0.0 48-49 0.0 0.0 0.0 0.2014071924811919 0.0 50-51 0.0 0.0 0.0 0.24718155440873552 0.0 52-53 0.0 0.0 0.0 0.32329988851727987 0.0 54-55 0.0 0.0 0.0 0.44733364341772064 0.0 56-57 0.0 0.0 0.0 0.624229403382873 0.0 58-59 0.0 0.0 0.0 0.8170723604066534 0.0 60-61 0.0 0.0 0.0 1.013902116695091 0.0 62-63 0.0 0.0 0.0 1.235612453579629 0.0 64-65 0.0 0.0 0.0 1.5130641505533529 0.0 66-67 0.0 0.0 0.0 1.8747554393969597 0.0 68-69 0.0 0.0 0.0 2.3024504049554437 0.0 70-71 0.0 0.0 0.0 2.753697018021809 0.0 72-73 0.0 0.0 0.0 3.185231123612926 0.0 74-75 0.0 0.0 0.0 3.683950179775115 0.0 76-77 0.0 0.0 0.0 4.253102689612911 0.0 78-79 0.0 0.0 0.0 4.839752818445591 0.0 80-81 0.0 0.0 0.0 5.461176696420003 0.0 82-83 0.0 0.0 0.0 6.101648615325551 0.0 84-85 0.0 0.0 0.0 6.807902722097943 0.0 86-87 0.0 0.0 0.0 7.594335791859546 0.0 88-89 0.0 0.0 0.0 8.49830561031252 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACCCG 35 2.7875838E-4 54.283897 5 GGTATCA 2305 0.0 53.4147 1 GTATCAA 4130 0.0 37.983578 1 GACATAT 290 0.0 32.784153 1 TCAACGC 5310 0.0 29.429337 4 ATCAACG 5330 0.0 29.318909 3 CAACGCA 5380 0.0 29.046427 5 CGCAGAA 100 4.94878E-5 28.501152 9 AACGCAG 5540 0.0 28.293278 6 CCTGTAC 85 6.4056914E-4 27.940243 3 TATCAAC 5610 0.0 27.855576 2 CTGTACT 120 5.3787917E-6 27.707409 4 CATATAG 330 0.0 27.34757 3 GCAGCTC 70 0.008387526 27.143953 8 GGGATGC 145 7.8767516E-7 26.207956 7 CATTCCG 165 8.396273E-8 25.910137 9 GGTTACT 110 9.468393E-5 25.910137 8 CCCTGGC 170 1.1209704E-7 25.148075 7 ATGGCGG 85 1.1234988E-7 25.14622 30-31 ACGCAGA 6330 0.0 24.61395 7 >>END_MODULE