FastQCFastQC Report
Thu 26 May 2016
SRR937171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220500
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38951.7664399092970522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27651.253968253968254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19930.9038548752834467No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.2870748299319728No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.25306122448979596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4880.22131519274376418No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.17188208616780046No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3720.16870748299319727No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.15374149659863945No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.13424036281179139No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.1328798185941043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9200.063.066461
GATAGGT250.005018647356.992087
GTATCAA19250.050.1524051
GGTCGCG250.001615832837.9947216-17
ATGGTCG250.001615832837.9947214-15
GTCGCGT250.001616736137.990418-19
TATCAAC26400.036.3644682
ATCAACG27600.034.78343
GTGGTAT3350.034.071631
TCAACGC28200.034.043334
TGCACTG1404.4929038E-1033.9470255
CAACGCA28900.033.218755
AACGCAG29300.032.7428936
CCTAAGA753.0606298E-431.6838913
TCCTAAG1051.884413E-631.6838912
CTCCTAA953.4491182E-530.036831
TGGTATC3650.029.9477862
CTAAGAG804.4676944E-429.7036484
ACTAATC650.0058184429.2466683
CCGACCC1004.916876E-528.5155014
Produced by FastQC (version 0.11.5)