##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937171_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 220500 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13497959183673 33.0 31.0 34.0 31.0 34.0 2 32.258385487528344 33.0 31.0 34.0 31.0 34.0 3 32.239224489795916 34.0 31.0 34.0 31.0 34.0 4 35.882340136054424 37.0 35.0 37.0 35.0 37.0 5 35.90926077097506 37.0 35.0 37.0 35.0 37.0 6 35.996331065759634 37.0 37.0 37.0 35.0 37.0 7 35.95656235827664 37.0 37.0 37.0 35.0 37.0 8 35.95224943310657 37.0 37.0 37.0 35.0 37.0 9 37.62369614512472 39.0 38.0 39.0 35.0 39.0 10-11 37.55531519274376 39.0 38.0 39.0 35.0 39.0 12-13 37.43538775510204 39.0 37.0 39.0 35.0 39.0 14-15 38.56824489795919 40.0 38.0 41.0 34.5 41.0 16-17 38.54333106575964 40.0 38.0 41.0 35.5 41.0 18-19 38.60015192743764 40.0 38.0 41.0 35.0 41.0 20-21 38.58407482993197 40.0 38.0 41.0 35.0 41.0 22-23 38.583562358276644 40.0 38.0 41.0 35.0 41.0 24-25 38.54268480725624 40.0 38.0 41.0 35.0 41.0 26-27 38.43642176870748 40.0 38.0 41.0 35.0 41.0 28-29 38.26968480725624 40.0 38.0 41.0 34.5 41.0 30-31 37.90226077097506 40.0 37.5 41.0 34.0 41.0 32-33 37.512841269841275 40.0 36.5 41.0 33.5 41.0 34-35 37.441723356009064 40.0 36.0 41.0 33.5 41.0 36-37 37.26179138321996 40.0 36.0 41.0 33.0 41.0 38-39 37.08091836734694 40.0 35.0 41.0 33.0 41.0 40-41 36.76287301587301 40.0 35.0 41.0 32.5 41.0 42-43 36.37739229024943 39.0 35.0 41.0 31.0 41.0 44-45 35.90925623582766 39.0 35.0 41.0 30.5 41.0 46-47 35.20775963718821 39.0 35.0 41.0 25.5 41.0 48-49 34.62599319727891 39.0 35.0 41.0 21.0 41.0 50-51 33.55030158730159 38.0 34.0 39.5 12.5 40.5 52-53 33.564126984126986 38.0 34.5 39.5 7.5 40.5 54-55 33.82743083900227 39.0 35.0 40.0 2.0 41.0 56-57 33.585569160997736 38.0 34.5 40.0 2.0 41.0 58-59 33.32354421768707 38.0 34.0 40.0 2.0 41.0 60-61 33.0000566893424 38.0 34.0 40.0 2.0 41.0 62-63 32.61291156462585 37.5 33.5 40.0 2.0 41.0 64-65 32.19528344671202 37.0 33.0 40.0 2.0 41.0 66-67 31.752526077097507 36.5 32.5 39.5 2.0 41.0 68-69 31.247668934240366 36.0 31.5 39.0 2.0 41.0 70-71 30.784038548752832 35.5 31.0 39.0 2.0 40.0 72-73 30.297249433106575 35.0 31.0 38.5 2.0 40.0 74-75 29.778820861678007 35.0 30.0 37.5 2.0 39.5 76-77 29.27602947845805 35.0 29.0 37.0 2.0 39.0 78-79 28.76768707482993 34.5 29.0 36.5 2.0 39.0 80-81 28.24366893424036 34.0 28.0 36.0 2.0 38.5 82-83 27.71261678004535 34.0 26.5 35.5 2.0 37.0 84-85 27.22143537414966 34.0 26.0 35.0 2.0 37.0 86-87 26.682709750566893 34.0 25.0 35.0 2.0 36.0 88-89 26.17791836734694 33.5 23.5 35.0 2.0 36.0 90-91 25.632215419501136 33.0 19.5 35.0 2.0 35.5 92-93 25.014442176870748 32.5 14.5 35.0 2.0 35.0 94-95 24.294612244897962 32.0 3.5 35.0 2.0 35.0 96-97 23.539 31.5 2.0 34.0 2.0 35.0 98-99 22.47789342403628 31.0 2.0 34.0 2.0 35.0 100-101 19.663102040816327 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.019621938306947584 1101 2 0.08224589125308768 1101 3 -0.10263069678647696 1101 4 -0.0015465366682008153 1101 5 0.027522578937514197 1101 6 0.10379573102559192 1101 7 0.12530365359809537 1101 8 0.11261589472526623 1101 9 0.2090242654536354 1101 10-11 0.29320460704428086 1101 12-13 0.2311998302150684 1101 14-15 0.13796143325176757 1101 16-17 0.2324371104785783 1101 18-19 0.3025631255619601 1101 20-21 0.227745370974894 1101 22-23 0.22308606459156977 1101 24-25 0.26568040643603297 1101 26-27 0.2587835321071168 1101 28-29 0.22464330171609248 1101 30-31 0.12549369380958098 1101 32-33 0.07728330332034972 1101 34-35 0.06652235007570795 1101 36-37 0.10067077314131012 1101 38-39 0.2664362967287346 1101 40-41 0.5416794382184662 1101 42-43 0.6327810254724469 1101 44-45 0.6342310010345642 1101 46-47 1.0350418094916662 1101 48-49 1.0984577057292242 1101 50-51 1.3018204720291209 1101 52-53 1.1112123350090997 1101 54-55 1.1924602948212524 1101 56-57 1.4800705978136506 1101 58-59 1.3418944766353604 1101 60-61 1.4993713475757495 1101 62-63 1.5763868577855078 1101 64-65 1.534543717480215 1101 66-67 1.9080593524695892 1101 68-69 1.6220039144540017 1101 70-71 1.9440419339929171 1101 72-73 1.9104298801342523 1101 74-75 2.018691884296885 1101 76-77 1.9745583909341917 1101 78-79 1.8707070268720365 1101 80-81 2.163583169896768 1101 82-83 2.1137161221687 1101 84-85 2.1620677274693207 1101 86-87 2.0961705906266417 1101 88-89 2.451418231749795 1101 90-91 2.267708236114373 1101 92-93 2.2938710106872264 1101 94-95 2.4963359333871047 1101 96-97 2.5799123218764635 1101 98-99 3.3555087154791075 1101 100-101 3.405269883377759 1102 1 0.1927877047069444 1102 2 0.24533000587359766 1102 3 0.09310091057638914 1102 4 0.1357537790194101 1102 5 0.3433952674643592 1102 6 0.2226782920611754 1102 7 0.26320150195170555 1102 8 0.17301428508859829 1102 9 0.2245495389702512 1102 10-11 0.21618899187646434 1102 12-13 0.1976983416713054 1102 14-15 0.33879586382429494 1102 16-17 0.31704350566447914 1102 18-19 0.25116209653668164 1102 20-21 0.2953961157755671 1102 22-23 0.4591590587590204 1102 24-25 0.502739593897374 1102 26-27 0.5673642069488736 1102 28-29 0.5540741650714764 1102 30-31 0.682357123224044 1102 32-33 0.6254428498300726 1102 34-35 0.8860595530940358 1102 36-37 1.179722229693283 1102 38-39 1.2021407242871547 1102 40-41 1.0916765298736308 1102 42-43 1.1200477629969683 1102 44-45 1.3947631752602803 1102 46-47 1.7236585478093716 1102 48-49 1.87826427435823 1102 50-51 1.7908507241033007 1102 52-53 2.2361918443977657 1102 54-55 2.4804657519337425 1102 56-57 2.4353296784594036 1102 58-59 1.9470866653499215 1102 60-61 2.2359466922146147 1102 62-63 2.3172929922779417 1102 64-65 2.0489082970128685 1102 66-67 2.2584144349343838 1102 68-69 2.4505226151112858 1102 70-71 2.7844108178759264 1102 72-73 2.757041129612073 1102 74-75 2.553020929143564 1102 76-77 2.854414665820368 1102 78-79 2.957513188332765 1102 80-81 3.098420381904532 1102 82-83 2.87577385545762 1102 84-85 2.738345680629102 1102 86-87 3.004483697743627 1102 88-89 3.622116921937039 1102 90-91 3.411734760219261 1102 92-93 3.7683621825386773 1102 94-95 3.7724251085276315 1102 96-97 3.801353697576449 1102 98-99 3.6138358355302955 1102 100-101 3.140938353218221 1103 1 0.21276039673481506 1103 2 0.06769862363175605 1103 3 0.05305586238878135 1103 4 0.11648870232786379 1103 5 0.14085781866680946 1103 6 -0.04391329429281399 1103 7 0.1368373491466599 1103 8 0.10123617731180445 1103 9 0.41976167407494813 1103 10-11 0.14156547313462653 1103 12-13 0.14547433656849762 1103 14-15 0.3242199845374856 1103 16-17 0.08648050298351251 1103 18-19 -0.4000134418987926 1103 20-21 -0.05920388223115225 1103 22-23 0.0693975550265904 1103 24-25 0.020041287190970536 1103 26-27 -0.21810874918894285 1103 28-29 -0.04537568892056498 1103 30-31 -0.20472341536092387 1103 32-33 0.34561586676300493 1103 34-35 0.37467292602725166 1103 36-37 0.42952040982623885 1103 38-39 0.7812291961370192 1103 40-41 0.582029041908072 1103 42-43 0.9150486101420938 1103 44-45 1.0734609637131953 1103 46-47 1.34814901493516 1103 48-49 1.8875479882424386 1103 50-51 1.6160348535869389 1103 52-53 1.658927226161218 1103 54-55 1.8411701780178404 1103 56-57 1.1724406146046036 1103 58-59 1.5403722927854702 1103 60-61 1.596212596118459 1103 62-63 1.523099424601014 1103 64-65 1.890178217381223 1103 66-67 2.0608203270778596 1103 68-69 2.1208087508338984 1103 70-71 2.33631589775673 1103 72-73 2.015963561018232 1103 74-75 1.926601961065984 1103 76-77 2.1642704518215474 1103 78-79 1.9384574063181539 1103 80-81 1.646528258360874 1103 82-83 2.1819321021662113 1103 84-85 2.37159164174016 1103 86-87 2.2849734472876655 1103 88-89 2.7851475088591116 1103 90-91 2.319719850465134 1103 92-93 2.7384828758023794 1103 94-95 2.5173796118514282 1103 96-97 1.760715647804183 1103 98-99 1.4147866313483881 1103 100-101 2.3981465615561213 1104 1 -0.12118012371128373 1104 2 -0.27521959198162804 1104 3 -0.14716718593835054 1104 4 0.1459682562312068 1104 5 0.005727707142646921 1104 6 0.046458452919083015 1104 7 0.02966262795706598 1104 8 0.02867112154972773 1104 9 -0.050758772021708864 1104 10-11 -0.06887132983935373 1104 12-13 -0.08856841436086427 1104 14-15 0.27399693621035226 1104 16-17 -0.09351949701648721 1104 18-19 0.0092988257592026 1104 20-21 -0.07664812014936473 1104 22-23 0.11383064052845526 1104 24-25 -0.30508882433319684 1104 26-27 0.18844329542072558 1104 28-29 -0.2500411164298626 1104 30-31 -0.07029032985905559 1104 32-33 -0.0717354343522345 1104 34-35 0.21128073271869852 1104 36-37 0.2761096291570908 1104 38-39 -0.04877080386297905 1104 40-41 0.33605320547684414 1104 42-43 -0.008752505099550945 1104 44-45 0.4065929339734211 1104 46-47 0.8039668588013313 1104 48-49 0.3719438990231083 1104 50-51 0.7945571584196571 1104 52-53 0.5862320588749768 1104 54-55 0.6799898806200702 1104 56-57 0.7008792763146303 1104 58-59 1.4382998020791504 1104 60-61 1.5173743061556344 1104 62-63 1.7080436625192306 1104 64-65 1.9637005222139372 1104 66-67 1.510653040832505 1104 68-69 1.4929555909826 1104 70-71 1.86342556318424 1104 72-73 1.7009821483788272 1104 74-75 1.1234699908057593 1104 76-77 0.9723838347211746 1104 78-79 1.195455547582096 1104 80-81 1.264372124531878 1104 82-83 1.3388837750286626 1104 84-85 1.0373537235245465 1104 86-87 1.3057169417114558 1104 88-89 1.3533166538405261 1104 90-91 1.2551194787179227 1104 92-93 1.3098769278469895 1104 94-95 0.999610095122808 1104 96-97 1.311273268622024 1104 98-99 2.0157996425008022 1104 100-101 2.4527748143442274 1105 1 -0.2290942341407316 1105 2 -0.10886989873009156 1105 3 -0.0967377380855936 1105 4 -0.2718845045049889 1105 5 -0.04672628058741424 1105 6 -0.1459955348109787 1105 7 -0.5506441205091903 1105 8 -0.30850679256070634 1105 9 -0.2876299376658764 1105 10-11 -0.39615660591297086 1105 12-13 -0.5910070646676147 1105 14-15 -0.5679049042804465 1105 16-17 -0.8644397424152643 1105 18-19 -0.45287908835122437 1105 20-21 -0.6567248072659311 1105 22-23 -0.47185954352062964 1105 24-25 -0.41600906973197027 1105 26-27 -0.22589719537682384 1105 28-29 -0.5936914231783277 1105 30-31 -1.0864713114541544 1105 32-33 -0.4553397288307579 1105 34-35 -0.058857304091112894 1105 36-37 -0.3651633769778826 1105 38-39 -0.24331068116359233 1105 40-41 0.23343050609034321 1105 42-43 0.4134407464341976 1105 44-45 0.9661788235439701 1105 46-47 0.9014821962246415 1105 48-49 -0.026322972142537537 1105 50-51 0.2954313915484832 1105 52-53 0.5830725496725151 1105 54-55 1.3296984695771243 1105 56-57 1.2557872517747555 1105 58-59 1.0401556303000064 1105 60-61 1.3416227724133059 1105 62-63 1.240252251476285 1105 64-65 1.2045440804961451 1105 66-67 1.1969413843907937 1105 68-69 0.9644126461973244 1105 70-71 1.1692691214664457 1105 72-73 1.1715956453113456 1105 74-75 1.2210006656523866 1105 76-77 1.6467550003653457 1105 78-79 0.941912602796819 1105 80-81 0.34167273802881226 1105 82-83 0.9098040204274369 1105 84-85 1.2862494290460198 1105 86-87 1.0593365736132974 1105 88-89 1.1271663470920572 1105 90-91 1.0829875768774286 1105 92-93 1.4292941057610982 1105 94-95 1.328199052178018 1105 96-97 0.9296781765974842 1105 98-99 0.9335450412738098 1105 100-101 0.8621919922583352 1106 1 -0.3434878160189747 1106 2 -0.8779118996739363 1106 3 -0.5225518013229689 1106 4 -0.3471086668457133 1106 5 -0.24427229285735308 1106 6 -0.5589261624655322 1106 7 -0.3157219874275512 1106 8 -0.17594426306565936 1106 9 0.1946258433629069 1106 10-11 -0.4821405606085847 1106 12-13 -0.49029918359163105 1106 14-15 -0.7629261407127288 1106 16-17 -0.4289041124011064 1106 18-19 -0.10877809731772459 1106 20-21 -0.05184042784167531 1106 22-23 -0.3894385902407791 1106 24-25 -0.17662728587166043 1106 26-27 -0.3509797815023532 1106 28-29 -0.1319641933529354 1106 30-31 -0.498174945243683 1106 32-33 -0.021927742044546505 1106 34-35 -0.35083465189668317 1106 36-37 -0.9702365246890636 1106 38-39 -0.7380015730937544 1106 40-41 -0.5949083329846943 1106 42-43 -0.69779096663801 1106 44-45 -0.4828301429496591 1106 46-47 -0.9271869873525205 1106 48-49 -0.5390176394384341 1106 50-51 -1.4233274569649623 1106 52-53 -1.6303064026634857 1106 54-55 -1.1948178116876207 1106 56-57 -1.0914284159930574 1106 58-59 -1.4942001979208541 1106 60-61 -1.468971847690522 1106 62-63 -1.615033260557695 1106 64-65 -1.0453379393245221 1106 66-67 -0.9689623437828736 1106 68-69 -0.8745444090173997 1106 70-71 -0.24984366758499377 1106 72-73 0.005597532994215726 1106 74-75 0.021354606190378433 1106 76-77 0.07603768087501805 1106 78-79 0.16449400912055623 1106 80-81 -0.7317817216219709 1106 82-83 -0.4872700711251845 1106 84-85 -0.572646276475453 1106 86-87 -0.8027445967500846 1106 88-89 -0.48418334615947245 1106 90-91 -0.36661129051285 1106 92-93 -0.41589230292224144 1106 94-95 -0.46115913564642597 1106 96-97 -1.442572885224127 1106 98-99 -1.1751618959607342 1106 100-101 -0.540686724117311 1107 1 0.38006987628871514 1107 2 0.5360304080183695 1107 3 0.37408281406164434 1107 4 0.5134682562312065 1107 5 0.2869777071426469 1107 6 0.22520845291908387 1107 7 0.2746626279570634 1107 8 0.20867112154972745 1107 9 0.5729912279782923 1107 10-11 0.5923786701606488 1107 12-13 0.6101815856391397 1107 14-15 0.46712193621035425 1107 16-17 0.5583555029835097 1107 18-19 0.40492382575919805 1107 20-21 0.39960187985062845 1107 22-23 0.6975806405284501 1107 24-25 0.478661175666808 1107 26-27 0.6009432954207199 1107 28-29 0.8293338835701363 1107 30-31 0.8422096701409387 1107 32-33 0.6076395656477658 1107 34-35 0.602530732718698 1107 36-37 0.9836096291570939 1107 38-39 0.8093541961370221 1107 40-41 0.5123032054768402 1107 42-43 0.5887474949004599 1107 44-45 0.9472179339734197 1107 46-47 0.5470918588013305 1107 48-49 0.13069389902310746 1107 50-51 0.18893215841965372 1107 52-53 0.2906070588749756 1107 54-55 0.19748988062007555 1107 56-57 0.444629276314636 1107 58-59 -0.5785751979208484 1107 60-61 -0.4745006938443659 1107 62-63 -0.7669563374807709 1107 64-65 -0.4275494777860622 1107 66-67 -0.5218469591674904 1107 68-69 -0.13079440901740114 1107 70-71 -0.5440744368157588 1107 72-73 -0.3352678516211718 1107 74-75 -0.47215500919423903 1107 76-77 -0.8794911652788251 1107 78-79 -1.0970444524179044 1107 80-81 -1.2293778754681242 1107 82-83 -1.6973662249713364 1107 84-85 -2.0945212764754544 1107 86-87 -1.458033058288546 1107 88-89 -1.1341833461594746 1107 90-91 -2.088005521282078 1107 92-93 -2.33512307215301 1107 94-95 -2.0647649048771974 1107 96-97 -1.3074767313779745 1107 98-99 -1.1273253574991955 1107 100-101 -0.2772251856557766 1108 1 0.3301758084921076 1108 2 0.38910244191667687 1108 3 0.27588366151927346 1108 4 0.41325639182442586 1108 5 0.4269141478206109 1108 6 0.37001777495297716 1108 7 0.3369507635502842 1108 8 0.3035863757870132 1108 9 0.24195309238507434 1108 10-11 0.4215100260928537 1108 12-13 0.678931585639134 1108 14-15 0.6995901565493341 1108 16-17 0.539870333491983 1108 18-19 0.2227734020303842 1108 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2316 74-75 -0.18948455464878577 2316 76-77 0.10366005849739679 2316 78-79 -0.07711438248783509 2316 80-81 0.22311338327313734 2316 82-83 0.3574851736300637 2316 84-85 0.3818991780700003 2316 86-87 0.6430595990541121 2316 88-89 0.918963506987378 2316 90-91 1.1047173808158206 2316 92-93 1.2355062984763592 2316 94-95 0.7804492559619653 2316 96-97 1.3553292126779688 2316 98-99 1.3279849571861178 2316 100-101 1.4481244646938762 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1131.0 3 134.0 4 118.0 5 119.0 6 134.0 7 166.0 8 202.0 9 250.0 10 341.0 11 499.0 12 840.0 13 1337.0 14 1433.0 15 2237.0 16 3203.0 17 4968.0 18 4156.0 19 2301.0 20 2066.0 21 1920.0 22 1887.0 23 1991.0 24 2282.0 25 2343.0 26 2395.0 27 2685.0 28 2982.0 29 3614.0 30 4373.0 31 5433.0 32 7277.0 33 9853.0 34 13916.0 35 21417.0 36 29800.0 37 42030.0 38 34219.0 39 4448.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.988123947763576 20.71392819766119 16.505892524002366 19.79205533057287 2 14.456395679363274 21.7259806708357 42.52006822057987 21.29755542922115 3 17.051185847147373 28.824158082634106 32.415126088637244 21.70952998158128 4 10.87942223819031 18.68175347798602 40.04220426317632 30.396620020647347 5 11.103025782596633 38.80487727229315 34.94817557088736 15.14392137422286 6 26.395159053815064 39.60092199839059 18.836863568788445 15.167055379005898 7 24.15348245135479 36.777141298417895 22.585924713584287 16.483451536643027 8 25.38382851037712 34.1265258802937 20.900638767020528 19.589006842308653 9 26.97874275321132 16.277367284301466 21.899738547232012 34.8441514152552 10-11 23.0131753953187 28.514556595086795 28.018006752532198 20.454261257062306 12-13 25.193354339730732 27.100071385376456 28.98087144721347 18.72570282767934 14-15 22.10750283009861 27.038466609382745 29.558959251126353 21.29507130939229 16-17 18.232323002714118 31.026181914066587 29.944171921386065 20.797323161833233 18-19 17.98022223282855 27.347501869364915 36.021809636149996 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17.996081533202414 28.316150265473855 34.60569859626155 19.082069605062184 46-47 18.270863499871567 28.630952516258674 33.89032981071515 19.207854173154605 48-49 18.252784723800865 28.91907251648102 33.247101614003185 19.581041145714938 50-51 18.370687656021936 29.059298566303355 32.66377471910368 19.906239058571032 52-53 18.592905401564096 29.23230159434033 32.44485983858753 19.729933165508033 54-55 18.294676205349937 29.181641899280052 32.360824109499355 20.162857785870656 56-57 18.42563954558573 29.208092919036723 32.21987142048773 20.146396114889818 58-59 18.4587524510862 28.55961337026341 32.637448280092975 20.34418589855741 60-61 18.531420066578047 28.509265255575627 32.82843087130895 20.130883806537373 62-63 18.370491229585124 28.17054076839696 33.250995889187585 20.207972112830326 64-65 18.456714383094752 27.925698704839807 33.12110883890025 20.49647807316519 66-67 18.37174793838211 28.036867113416818 32.86954031999672 20.72184462820434 68-69 18.28008543901109 27.696782403199414 32.910607162334124 21.112524995455374 70-71 18.322293297581037 27.56706395646098 33.03057502868894 21.080067717269042 72-73 18.51670889815583 27.207608485688638 32.978058563523355 21.297624052632177 74-75 18.090396301969463 27.498647782590712 32.6282106641092 21.782745251330628 76-77 17.897878375982508 26.99763152750565 33.09451614516327 22.009973951348574 78-79 18.079822888562084 26.865841267965152 33.0222366683411 22.032099175131663 80-81 17.950944116657727 27.089602443703008 32.72202878260316 22.2374246570361 82-83 17.94267560793261 27.064448469175584 32.44329711832785 22.549578804563954 84-85 17.861070214660923 26.812394350837014 32.432929821691474 22.893605612810585 86-87 17.85726458244812 26.647519546649516 32.39301887521274 23.102196995689624 88-89 18.101696764193868 26.438476957551348 32.21465680637434 23.245169471880438 90-91 18.576931380458372 26.585612508095945 31.887236242571614 22.950219868874065 92-93 18.58561938807744 26.56792940369026 31.542442099617517 23.304009108614775 94-95 18.719661848922826 26.473047904735935 31.161939823652396 23.645350422688846 96-97 19.53129794241115 26.721835715633173 30.341992040565387 23.404874301390286 98-99 19.926130487652426 27.115338720125465 29.40824838339413 23.550282408827975 100-101 20.748813292038093 26.204829543517928 29.24811910480523 23.79823805963875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 559.0 1 356.5 2 229.0 3 287.5 4 217.0 5 211.5 6 408.5 7 865.0 8 1515.0 9 1718.5 10 1287.5 11 924.5 12 882.5 13 893.0 14 950.0 15 937.5 16 909.0 17 993.5 18 1034.0 19 1050.5 20 1102.0 21 1222.0 22 1370.0 23 1565.0 24 1779.0 25 1984.5 26 2358.5 27 2799.0 28 3012.5 29 3255.0 30 3743.0 31 4279.5 32 4829.0 33 5508.0 34 6189.5 35 6645.5 36 7161.0 37 7637.5 38 7737.5 39 7608.5 40 7518.5 41 7631.5 42 7661.0 43 7776.0 44 7815.0 45 7808.5 46 7541.5 47 7148.5 48 6972.5 49 6560.0 50 6114.5 51 5595.5 52 5071.5 53 4735.5 54 4456.0 55 4028.5 56 3537.5 57 2967.5 58 2483.5 59 2094.0 60 1686.0 61 1423.0 62 1143.5 63 819.0 64 575.5 65 422.5 66 301.0 67 234.5 68 182.5 69 116.0 70 83.5 71 63.0 72 47.5 73 37.5 74 34.0 75 25.5 76 20.0 77 13.5 78 4.5 79 4.0 80 3.0 81 0.0 82 0.0 83 1.5 84 2.0 85 1.0 86 0.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3310657596371882 2 0.2834467120181406 3 0.2789115646258503 4 0.2798185941043084 5 0.28253968253968254 6 0.24625850340136055 7 0.24489795918367346 8 0.24716553287981857 9 0.26077097505668934 10-11 0.264172335600907 12-13 0.2571428571428571 14-15 0.24625850340136055 16-17 0.24444444444444444 18-19 0.22925170068027212 20-21 0.23265306122448978 22-23 0.2342403628117914 24-25 0.23287981859410428 26-27 0.23333333333333336 28-29 0.24580498866213155 30-31 0.2630385487528345 32-33 0.2526077097505669 34-35 0.2496598639455782 36-37 0.23854875283446714 38-39 0.22517006802721087 40-41 0.22108843537414968 42-43 0.23786848072562355 44-45 0.23401360544217686 46-47 0.24512471655328796 48-49 0.2494331065759637 50-51 0.26258503401360545 52-53 0.28503401360544217 54-55 0.2510204081632653 56-57 0.21791383219954646 58-59 0.2015873015873016 60-61 0.20748299319727892 62-63 0.21337868480725622 64-65 0.20408163265306123 66-67 0.21156462585034014 68-69 0.20861678004535145 70-71 0.21201814058956917 72-73 0.21882086167800455 74-75 0.22267573696145124 76-77 0.23900226757369614 78-79 0.23832199546485264 80-81 0.22947845804988662 82-83 0.21383219954648527 84-85 0.19591836734693877 86-87 0.2038548752834467 88-89 0.22358276643990932 90-91 0.21882086167800455 92-93 0.22154195011337868 94-95 0.21768707482993196 96-97 0.23106575963718823 98-99 0.2346938775510204 100-101 0.20793650793650792 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 220500.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.29996407611859 #Duplication Level Percentage of deduplicated Percentage of total 1 78.83562646974472 44.38442938164932 2 12.019877969593399 13.534373957748752 3 3.5075499797794683 5.924248135703237 4 1.5410810827546322 3.4705123838988694 5 0.9367229823345384 2.636873512735459 6 0.6184727588613359 2.08919964635707 7 0.4543086451171143 1.7904292279694554 8 0.3586054580511351 1.6151579524623167 9 0.24626946442937603 1.2478465800376952 >10 1.3997279736885904 13.463872057046503 >50 0.0561465215880182 2.1140196017048654 >100 0.02068556058505934 2.269853680424453 >500 0.0019700533890532703 0.6604924831139808 >1k 0.0029550800835799057 4.798691399148006 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3895 1.7664399092970522 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2765 1.253968253968254 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1993 0.9038548752834467 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 633 0.2870748299319728 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 558 0.25306122448979596 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 488 0.22131519274376418 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 379 0.17188208616780046 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 372 0.16870748299319727 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.15374149659863945 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 296 0.13424036281179139 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 293 0.1328798185941043 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.5351473922902497E-4 0.0 18-19 0.0 0.0 0.0 4.5351473922902497E-4 0.0 20-21 0.0 0.0 0.0 4.5351473922902497E-4 0.0 22-23 0.0 0.0 0.0 0.0015873015873015873 0.0 24-25 0.0 0.0 0.0 0.0027210884353741495 0.0 26-27 0.0 0.0 0.0 0.0058956916099773245 0.0 28-29 0.0 0.0 0.0 0.011791383219954649 0.0 30-31 0.0 0.0 0.0 0.029705215419501135 0.0 32-33 0.0 0.0 0.0 0.05782312925170068 0.0 34-35 0.0 0.0 0.0 0.0981859410430839 0.0 36-37 0.0 0.0 0.0 0.1712018140589569 0.0 38-39 0.0 0.0 0.0 0.2868480725623583 0.0 40-41 0.0 0.0 0.0 0.42380952380952386 0.0 42-43 0.0 0.0 0.0 0.5893424036281179 0.0 44-45 0.0 0.0 0.0 0.7959183673469388 0.0 46-47 0.0 0.0 0.0 1.0662131519274376 0.0 48-49 0.0 0.0 0.0 1.3741496598639458 0.0 50-51 0.0 0.0 0.0 1.7401360544217686 0.0 52-53 0.0 0.0 0.0 2.1052154195011337 0.0 54-55 0.0 0.0 0.0 2.512471655328798 0.0 56-57 0.0 0.0 0.0 3.0172335600907028 0.0 58-59 0.0 0.0 0.0 3.5621315192743763 0.0 60-61 0.0 0.0 0.0 4.159863945578231 0.0 62-63 0.0 0.0 0.0 4.852607709750567 0.0 64-65 0.0 0.0 0.0 5.554648526077097 0.0 66-67 0.0 0.0 0.0 6.355102040816327 0.0 68-69 0.0 0.0 0.0 7.326077097505669 0.0 70-71 0.0 0.0 0.0 8.596145124716553 0.0 72-73 0.0 0.0 0.0 9.829478458049886 0.0 74-75 0.0 0.0 0.0 10.987301587301587 0.0 76-77 0.0 0.0 0.0 12.407936507936508 0.0 78-79 0.0 0.0 0.0 13.910884353741498 0.0 80-81 0.0 0.0 0.0 15.418367346938776 0.0 82-83 0.0 0.0 0.0 16.876190476190477 0.0 84-85 0.0 0.0 0.0 18.348299319727893 0.0 86-87 0.0 0.0 0.0 19.8156462585034 0.0 88-89 0.0 0.0 0.0 21.629478458049885 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 920 0.0 63.06646 1 GATAGGT 25 0.0050186473 56.99208 7 GTATCAA 1925 0.0 50.152405 1 GGTCGCG 25 0.0016158328 37.99472 16-17 ATGGTCG 25 0.0016158328 37.99472 14-15 GTCGCGT 25 0.0016167361 37.9904 18-19 TATCAAC 2640 0.0 36.364468 2 ATCAACG 2760 0.0 34.7834 3 GTGGTAT 335 0.0 34.07163 1 TCAACGC 2820 0.0 34.04333 4 TGCACTG 140 4.4929038E-10 33.947025 5 CAACGCA 2890 0.0 33.21875 5 AACGCAG 2930 0.0 32.742893 6 CCTAAGA 75 3.0606298E-4 31.683891 3 TCCTAAG 105 1.884413E-6 31.683891 2 CTCCTAA 95 3.4491182E-5 30.03683 1 TGGTATC 365 0.0 29.947786 2 CTAAGAG 80 4.4676944E-4 29.703648 4 ACTAATC 65 0.00581844 29.246668 3 CCGACCC 100 4.916876E-5 28.515501 4 >>END_MODULE