FastQCFastQC Report
Thu 26 May 2016
SRR937171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220500
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29021.3160997732426305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24741.1219954648526078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18790.8521541950113379No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10920.4952380952380952No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.24535147392290246No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.19909297052154196No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.1523809523809524No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.11247165532879819No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG200.002073359271.23695
GGTATCA9700.063.6769751
GTATCAA18600.044.4474751
ACCGTCT550.00257171834.554788
TATCAAC25700.032.3384132
ATCAACG26350.031.7209243
GTCTAAG753.0655842E-431.6752151
AGGTGTC600.003938880731.6752137
CATATAG600.00394764131.6608493
TAGGACC902.4072162E-531.6608474
TCAACGC26700.031.3051054
CAACGCA27000.030.9572725
AACGCAG27650.030.4012836
GTGGTAT3850.029.618381
GAGTTAC650.00582627629.2386571
TGGTATC3900.029.2253952
TCTATAG856.3975167E-427.9360393
ACGCAGA30950.026.8650827
ATAGGAT1606.204937E-826.7138376
CGCAGAG30800.026.6873938