##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937171_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 220500 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.494503401360543 31.0 31.0 34.0 30.0 34.0 2 31.7224716553288 31.0 31.0 34.0 30.0 34.0 3 31.71968707482993 31.0 31.0 34.0 30.0 34.0 4 35.586185941043084 37.0 35.0 37.0 33.0 37.0 5 35.488249433106574 37.0 35.0 37.0 33.0 37.0 6 35.551102040816325 37.0 35.0 37.0 33.0 37.0 7 35.460253968253966 37.0 35.0 37.0 33.0 37.0 8 35.44114285714286 37.0 35.0 37.0 33.0 37.0 9 36.97472562358276 39.0 37.0 39.0 33.0 39.0 10-11 36.87296825396825 39.0 37.0 39.0 33.0 39.0 12-13 36.71471428571429 38.5 36.0 39.0 32.0 39.0 14-15 37.603537414965984 39.0 36.5 41.0 32.0 41.0 16-17 37.56464852607709 39.0 36.5 40.0 32.0 41.0 18-19 37.66892743764173 39.0 37.0 40.5 32.0 41.0 20-21 37.61986167800454 39.0 36.5 40.5 32.0 41.0 22-23 37.524360544217686 39.0 36.0 40.5 32.0 41.0 24-25 37.504435374149665 39.0 36.0 41.0 32.0 41.0 26-27 37.23113605442177 39.0 36.0 40.0 31.5 41.0 28-29 37.010229024943314 39.0 36.0 40.0 31.0 41.0 30-31 36.846911564625856 38.5 35.5 40.0 31.0 41.0 32-33 36.545748299319726 38.0 35.0 40.0 31.0 41.0 34-35 36.27751927437642 38.0 35.0 40.0 30.0 41.0 36-37 35.995759637188215 38.0 35.0 40.0 30.0 41.0 38-39 35.742879818594105 38.0 35.0 40.0 30.0 41.0 40-41 35.389369614512475 38.0 35.0 40.0 29.0 41.0 42-43 34.96932653061225 38.0 34.0 40.0 26.5 41.0 44-45 34.33204535147392 38.0 33.5 40.0 24.5 41.0 46-47 33.60098412698413 38.0 33.0 40.0 21.0 41.0 48-49 32.94420181405896 37.0 33.0 40.0 13.5 41.0 50-51 32.52446031746032 37.0 32.0 40.0 8.5 41.0 52-53 32.159671201814064 37.0 31.0 40.0 4.5 41.0 54-55 31.8373514739229 36.0 31.0 39.0 2.0 40.5 56-57 31.41434240362812 36.0 31.0 39.0 2.0 40.0 58-59 31.044176870748302 35.5 30.0 39.0 2.0 40.0 60-61 30.578965986394557 35.0 29.0 39.0 2.0 40.0 62-63 30.08347619047619 35.0 28.0 38.0 2.0 40.0 64-65 29.54054648526077 34.5 28.0 38.0 2.0 40.0 66-67 28.982031746031744 34.0 26.5 38.0 2.0 40.0 68-69 28.38477097505669 34.0 26.0 37.0 2.0 39.0 70-71 27.884290249433107 33.5 25.5 36.5 2.0 39.0 72-73 27.298242630385488 33.0 24.5 36.0 2.0 39.0 74-75 26.741979591836735 32.5 22.5 35.5 2.0 38.0 76-77 25.675403628117913 30.5 21.5 34.5 2.0 36.5 78-79 26.279544217687075 32.0 23.0 35.0 2.0 37.0 80-81 26.02536507936508 32.0 22.0 35.0 2.0 37.0 82-83 25.518292517006802 32.0 20.0 35.0 2.0 36.0 84-85 24.970124716553286 31.5 18.0 34.5 2.0 36.0 86-87 24.456655328798185 31.0 14.5 34.0 2.0 35.0 88-89 23.824340136054424 31.0 7.0 34.0 2.0 35.0 90-91 23.026460317460316 30.0 2.0 34.0 2.0 35.0 92-93 22.233707482993196 29.5 2.0 34.0 2.0 35.0 94-95 21.461058956916098 29.0 2.0 34.0 2.0 35.0 96-97 20.47528344671202 29.0 2.0 34.0 2.0 35.0 98-99 19.13528798185941 26.5 2.0 33.0 2.0 34.5 100-101 16.567038548752834 20.5 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13609985389501844 1101 2 0.27196758429704104 1101 3 0.13907178690746846 1101 4 0.17828058293716964 1101 5 0.00465063996607995 1101 6 0.2229683010470751 1101 7 0.04824680822304117 1101 8 -0.09355949242306139 1101 9 -0.018431054477915154 1101 10-11 -0.05242122882249589 1101 12-13 0.1271422194316898 1101 14-15 0.2954179277573843 1101 16-17 0.04119978154194115 1101 18-19 -0.15323781185332308 1101 20-21 -0.289736936207035 1101 22-23 0.02757451255393306 1101 24-25 0.015911708287205784 1101 26-27 -0.07131999448261439 1101 28-29 -0.43379580279919594 1101 30-31 0.334668185936259 1101 32-33 0.21647116532866306 1101 34-35 0.23136440823631688 1101 36-37 0.19622650580824796 1101 38-39 0.5241055810336519 1101 40-41 0.4710350754126651 1101 42-43 0.3222985558773175 1101 44-45 0.5320745593588896 1101 46-47 0.499196107802085 1101 48-49 0.5977983041856589 1101 50-51 0.6863325185839777 1101 52-53 0.7211154627541774 1101 54-55 0.48179741690931976 1101 56-57 0.6706931712383515 1101 58-59 1.1698411960552164 1101 60-61 1.0478027486993504 1101 62-63 0.9536431955664852 1101 64-65 0.8508317985951628 1101 66-67 0.8120698407704339 1101 68-69 1.0309356732814834 1101 70-71 0.8914108075682101 1101 72-73 0.7050543407748329 1101 74-75 1.0907758226594986 1101 76-77 1.2702541706689665 1101 78-79 1.435415853643267 1101 80-81 1.1631034762300096 1101 82-83 1.0599072523680135 1101 84-85 1.1100746877537766 1101 86-87 1.2844550318594372 1101 88-89 1.50514303367612 1101 90-91 1.3589989477222382 1101 92-93 1.0620998876753802 1101 94-95 1.2478194886832732 1101 96-97 1.393992916642297 1101 98-99 1.8587107447385556 1101 100-101 1.494165111612201 1102 1 0.28017396909720205 1102 2 0.21564616460172914 1102 3 0.28814825522501764 1102 4 0.3199064005786667 1102 5 0.21572773225579311 1102 6 0.26382790202215745 1102 7 0.2936212179612383 1102 8 0.3982310432940608 1102 9 0.2725091869675822 1102 10-11 0.2749103383523419 1102 12-13 0.2751743275586662 1102 14-15 0.1326986782161086 1102 16-17 0.38510098242396396 1102 18-19 0.36012735463619805 1102 20-21 0.4540537475712725 1102 22-23 0.5501406245199192 1102 24-25 0.31871535270767737 1102 26-27 0.5149309375097104 1102 28-29 0.5779618421818 1102 30-31 0.8732354823917774 1102 32-33 1.049236049444339 1102 34-35 1.0236424883056898 1102 36-37 1.2442802943239926 1102 38-39 1.204264323782617 1102 40-41 1.391936027763407 1102 42-43 1.666339886101369 1102 44-45 2.063228220436244 1102 46-47 2.2113572195301145 1102 48-49 2.5276516707270034 1102 50-51 2.645504645007076 1102 52-53 2.9652287084141307 1102 54-55 2.6927238040576107 1102 56-57 3.1768271348893258 1102 58-59 3.3031993825171355 1102 60-61 3.2956651152459777 1102 62-63 2.968354132529427 1102 64-65 3.345557118645292 1102 66-67 3.264558061973858 1102 68-69 3.063548794675796 1102 70-71 3.2809624465527207 1102 72-73 4.014774558001751 1102 74-75 3.399872067721919 1102 76-77 2.8780506499853438 1102 78-79 3.384686387879775 1102 80-81 3.252521860142103 1102 82-83 3.1947645583946347 1102 84-85 3.3488868139653682 1102 86-87 3.762356899016819 1102 88-89 3.9058257015272737 1102 90-91 4.141193003065393 1102 92-93 3.8736602939975953 1102 94-95 3.91572616782938 1102 96-97 4.024675425856461 1102 98-99 3.9329898718120724 1102 100-101 4.158232775576746 1103 1 -0.049883736435152315 1103 2 -0.106767302015772 1103 3 -0.08391788189289429 1103 4 -0.038645280256922376 1103 5 -0.021034379427831595 1103 6 -0.06390762923749094 1103 7 0.19508329077584818 1103 8 -0.10908928719220512 1103 9 -0.029363502070360425 1103 10-11 0.08905168202416291 1103 12-13 -0.025901048424273654 1103 14-15 -0.022099651413043375 1103 16-17 0.27327782645956233 1103 18-19 -0.31829137424745824 1103 20-21 0.2154030005886014 1103 22-23 0.030412109615149063 1103 24-25 0.19696066600187834 1103 26-27 0.12248348542323839 1103 28-29 -0.23957804838698848 1103 30-31 -0.04174602991176357 1103 32-33 0.04649030261116849 1103 34-35 0.5332202113789393 1103 36-37 0.6508102882444788 1103 38-39 0.18846257567373925 1103 40-41 0.15527358021165583 1103 42-43 0.41897849399277476 1103 44-45 0.20462172432813475 1103 46-47 0.5258673204898869 1103 48-49 0.9189380356771224 1103 50-51 1.4483739753637757 1103 52-53 1.014044200206797 1103 54-55 0.4969386001946283 1103 56-57 1.3012079714700526 1103 58-59 1.2164965709919784 1103 60-61 1.169445475432152 1103 62-63 1.573793800847202 1103 64-65 1.5381132046355042 1103 66-67 1.1057831334828734 1103 68-69 1.6001305142807283 1103 70-71 1.1739680177776926 1103 72-73 1.191518929469904 1103 74-75 0.6815129379891225 1103 76-77 0.9224908863886689 1103 78-79 1.0931737853502987 1103 80-81 1.2574004193974133 1103 82-83 1.711014795096947 1103 84-85 1.1010669668501478 1103 86-87 0.8233015156587342 1103 88-89 1.61830963068601 1103 90-91 1.691005136176809 1103 92-93 1.6564921506407089 1103 94-95 2.0104768580034964 1103 96-97 2.3503877752390423 1103 98-99 2.7722116942703927 1103 100-101 2.9123513958265086 1104 1 -0.029196004093144268 1104 2 0.09650407344891221 1104 3 -0.20910375549884108 1104 4 -0.05191665572160531 1104 5 -0.05110872887021145 1104 6 0.27960538191491935 1104 7 -0.24342972037656807 1104 8 -0.04033464035205725 1104 9 -0.3078393385015872 1104 10-11 0.16814090135501658 1104 12-13 -0.2120441711008496 1104 14-15 -0.14787102687772347 1104 16-17 0.12048972237033695 1104 18-19 -0.09723189469355731 1104 20-21 0.2682561604399112 1104 22-23 0.1352076486486098 1104 24-25 0.15210007120634117 1104 26-27 -0.0649886335358687 1104 28-29 0.00948328990297398 1104 30-31 -0.2170527213615685 1104 32-33 0.07172264461860323 1104 34-35 0.1225231853566342 1104 36-37 0.5923902138950368 1104 38-39 0.8720592299488317 1104 40-41 0.9933033199886054 1104 42-43 0.6190342560745563 1104 44-45 0.787893099792818 1104 46-47 0.5409416699322591 1104 48-49 0.9924975152310296 1104 50-51 1.1978907039883104 1104 52-53 1.199927099835044 1104 54-55 1.0283605332801322 1104 56-57 0.7426113171949602 1104 58-59 0.49534415463509873 1104 60-61 0.6975216836105922 1104 62-63 0.72601499043828 1104 64-65 1.01461878084368 1104 66-67 1.1746585981668858 1104 68-69 1.4687364622361194 1104 70-71 1.112323036365055 1104 72-73 0.7228107510312434 1104 74-75 1.2023586628961844 1104 76-77 0.5606042692882944 1104 78-79 0.8981180232685126 1104 80-81 1.0000955866836598 1104 82-83 1.0963865423088421 1104 84-85 1.2808439185230114 1104 86-87 1.2739520732795526 1104 88-89 1.2162278462993932 1104 90-91 1.222928927998371 1104 92-93 1.570181741718777 1104 94-95 1.086034478821336 1104 96-97 1.2733617529342105 1104 98-99 1.157694965645856 1104 100-101 1.0682528828153544 1105 1 -0.11358250716062912 1105 2 0.2476697176206919 1105 3 -0.16784608678718627 1105 4 -0.1047387416111718 1105 5 -0.16503510923831044 1105 6 -0.16478112115256494 1105 7 -0.4831843216035594 1105 8 -0.1867456832968415 1105 9 -0.3043424059862474 1105 10-11 -0.5031781170498846 1105 12-13 -0.24758098091680836 1105 14-15 -0.15261029068140886 1105 16-17 0.14012162421083474 1105 18-19 0.29200123414079826 1105 20-21 -0.2324953733024202 1105 22-23 -0.10178621638207375 1105 24-25 0.08199270924314561 1105 26-27 -0.03363894028434089 1105 28-29 0.2016151917434712 1105 30-31 0.08050862833167827 1105 32-33 0.1606030127167628 1105 34-35 -0.02997681464336921 1105 36-37 0.0789239562263262 1105 38-39 -0.40531807066466286 1105 40-41 -0.3631997474960613 1105 42-43 -0.33805163349599354 1105 44-45 0.1296108912038605 1105 46-47 0.1299907496868613 1105 48-49 0.6304576379304123 1105 50-51 0.08905634816009211 1105 52-53 -0.5443520412692493 1105 54-55 0.3690507173292126 1105 56-57 0.31523401658146 1105 58-59 0.15626440003387287 1105 60-61 0.7302364075369709 1105 62-63 0.07060088000883269 1105 64-65 0.47866786059828215 1105 66-67 0.5243978619705665 1105 68-69 0.6853929039539111 1105 70-71 0.6520623001687333 1105 72-73 1.152120566982159 1105 74-75 0.7597052886630564 1105 76-77 0.5583496680613003 1105 78-79 1.285786734924958 1105 80-81 1.2381630713462357 1105 82-83 0.9739632294254044 1105 84-85 1.4880831823266938 1105 86-87 1.285761889230475 1105 88-89 1.5770867420049157 1105 90-91 2.213036289961561 1105 92-93 1.9819762202463806 1105 94-95 1.9831203683918908 1105 96-97 3.0459997897440267 1105 98-99 3.5149649042961606 1105 100-101 3.715108711036212 1106 1 -0.39111908101622106 1106 2 0.045734842679681975 1106 3 -0.14179606319115123 1106 4 -0.09883973264468437 1106 5 -0.07957026733175354 1106 6 -0.5807792334696913 1106 7 -0.8957374126842552 1106 8 -0.5107192557366673 1106 9 -0.31014703080927575 1106 10-11 -0.7107052524911381 1106 12-13 -0.9633903249469995 1106 14-15 -0.6515248730315761 1106 16-17 -0.1756641237835055 1106 18-19 -0.43223189469355816 1106 20-21 -0.5007823010985604 1106 22-23 -0.028638505197548625 1106 24-25 0.07498468659095181 1106 26-27 -0.32960401815125095 1106 28-29 -0.3449397870200954 1106 30-31 -0.05339887520772635 1106 32-33 0.23806879846475937 1106 34-35 -0.6338229684895254 1106 36-37 0.3081594446642626 1106 38-39 0.4730207684103789 1106 40-41 0.034841781527070737 1106 42-43 -0.723658051617754 1106 44-45 -0.11979920789949006 1106 46-47 -0.6232890992985105 1106 48-49 -0.3680794078458973 1106 50-51 0.1371214732190822 1106 52-53 -0.1648805924726453 1106 54-55 -0.5056779282583328 1106 56-57 -0.6448886828050426 1106 58-59 -0.36004046074951646 1106 60-61 -0.7519013933124832 1106 62-63 -0.48225424033095265 1106 64-65 -1.401535065310167 1106 66-67 -0.5151490941408028 1106 68-69 -0.17241738391772543 1106 70-71 -0.6182538867118694 1106 72-73 -0.165458479737989 1106 74-75 -0.16898749094997 1106 76-77 0.04579657698060302 1106 78-79 -0.09495889980840744 1106 80-81 -0.42259672100864876 1106 82-83 -0.6501519192296179 1106 84-85 -0.30530992763083376 1106 86-87 0.2604905348180182 1106 88-89 -0.8553106152390662 1106 90-91 -1.1568787643093223 1106 92-93 -1.6069336428966103 1106 94-95 -1.1928116750248172 1106 96-97 -0.5274074778350197 1106 98-99 0.08346419641508618 1106 100-101 -0.026939424876953666 1107 1 0.0045539959068534586 1107 2 -0.20474592655108736 1107 3 0.05339624450115821 1107 4 0.0968333442783944 1107 5 0.14639127112978656 1107 6 0.26585538191492475 1107 7 0.18407027962343392 1107 8 0.16091535964794446 1107 9 0.38216066149841055 1107 10-11 0.1837659013550237 1107 12-13 0.16233082889915096 1107 14-15 -0.04912102687772801 1107 16-17 -0.07326027762965737 1107 18-19 -0.07285689469355816 1107 20-21 0.2670061604399123 1107 22-23 0.13458264864860325 1107 24-25 0.01897507120633435 1107 26-27 0.026886366464133005 1107 28-29 -0.21176671009702375 1107 30-31 -0.1776777213615759 1107 32-33 -0.19390235538138967 1107 34-35 -0.5956018146433664 1107 36-37 -0.8538597861049695 1107 38-39 0.11893422994883451 1107 40-41 -0.5204466800113963 1107 42-43 -0.9409657439254389 1107 44-45 -1.0839819002071849 1107 46-47 -1.2290583300677405 1107 48-49 -1.716877484768979 1107 50-51 -1.8821092960116879 1107 52-53 -2.1631979001649526 1107 54-55 -1.9503894667198693 1107 56-57 -1.454263682805042 1107 58-59 -2.1227808453649004 1107 60-61 -1.75310331638941 1107 62-63 -2.6514850095617177 1107 64-65 -3.4772562191563203 1107 66-67 -2.754091401833115 1107 68-69 -2.4981385377638823 1107 70-71 -1.898301963634946 1107 72-73 -2.253439248968757 1107 74-75 -2.237016337103814 1107 76-77 -2.6212707307117036 1107 78-79 -2.612506976731485 1107 80-81 -2.9992794133163407 1107 82-83 -3.0198634576911587 1107 84-85 -2.359156081476989 1107 86-87 -1.9041729267204452 1107 88-89 -1.8718971537006048 1107 90-91 -1.8114460720016297 1107 92-93 -1.6723182582812264 1107 94-95 -1.3358405211786604 1107 96-97 -0.8935132470657905 1107 98-99 -0.4254300343541466 1107 100-101 0.36825288281535506 1108 1 -0.31250108883890704 1108 2 -0.02180101129684786 1108 3 -0.1849512131259594 1108 4 -0.1744590286029606 1108 5 -0.08517652548037802 1108 6 -0.5670895333393133 1108 7 -0.47122633054605956 1108 8 -0.5563515895045938 1108 9 -0.37546645714565585 1108 10-11 -1.1161811325432822 1108 12-13 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0.3242567361564568 2316 46-47 0.6941934181840068 2316 48-49 0.7494031096366172 2316 50-51 0.018590004687613515 2316 52-53 -0.22571975331180738 2316 54-55 0.45830808572768333 2316 56-57 0.4669994290830708 2316 58-59 -0.028921579630633687 2316 60-61 -0.14665663806772855 2316 62-63 0.3778856198089109 2316 64-65 1.3225908088157077 2316 66-67 0.28974601075429973 2316 68-69 0.5576525461522053 2316 70-71 0.7425852741272898 2316 72-73 1.0838422195627082 2316 74-75 0.8540894321269512 2316 76-77 1.5262161574001816 2316 78-79 1.3428033379538284 2316 80-81 1.6232074747955458 2316 82-83 1.2064914374137352 2316 84-85 1.2464383241174168 2316 86-87 1.409791234118714 2316 88-89 1.7688152588868071 2316 90-91 1.2343799769494197 2316 92-93 0.7203390843761142 2316 94-95 0.7939016116884723 2316 96-97 1.030984130556586 2316 98-99 1.1075900705409616 2316 100-101 1.4821514842139543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 6.0 5 8.0 6 17.0 7 76.0 8 166.0 9 313.0 10 503.0 11 820.0 12 1218.0 13 1609.0 14 2234.0 15 2993.0 16 4117.0 17 5546.0 18 4597.0 19 2828.0 20 2629.0 21 2764.0 22 2869.0 23 3079.0 24 3404.0 25 3929.0 26 4412.0 27 5158.0 28 6067.0 29 7098.0 30 8705.0 31 10179.0 32 12298.0 33 15175.0 34 19074.0 35 23692.0 36 27275.0 37 25845.0 38 9502.0 39 291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.015031283035476 23.068686566216137 17.240378027921075 18.675904122827312 2 15.925623582766441 24.43219954648526 39.153741496598634 20.48843537414966 3 18.08344671201814 29.537414965986397 32.66167800453515 19.71746031746032 4 12.426757369614512 20.904308390022674 38.459863945578235 28.20907029478458 5 11.43673469387755 40.250340136054426 34.25578231292517 14.057142857142857 6 26.74195011337869 40.93877551020408 17.401360544217688 14.917913832199547 7 24.07936507936508 37.782766439909295 21.476190476190478 16.661678004535148 8 26.265759637188207 35.43174603174603 19.902947845804988 18.39954648526077 9 26.88072562358277 16.993650793650794 21.218594104308387 34.90702947845805 10-11 23.774149659863948 29.595691609977326 26.840362811791387 19.789795918367346 12-13 25.527919886796564 28.148243895359386 28.14552265882951 18.17831355901455 14-15 22.363492063492064 28.27619047619048 28.633333333333333 20.726984126984128 16-17 18.639455782312925 32.0766439909297 28.977551020408164 20.306349206349207 18-19 18.536961451247166 28.481632653061222 34.7265306122449 18.25487528344671 20-21 19.872562358276642 27.40612244897959 35.182539682539684 17.538775510204083 22-23 19.661131481620057 27.78953144805974 34.988900151248195 17.56043691907201 24-25 19.09501133786848 28.171201814058954 35.05532879818594 17.678458049886622 26-27 18.68355888542196 28.601879399172773 35.07229156084464 17.642270154560627 28-29 18.341296603270422 28.500871915697427 35.382543091230275 17.775288389801876 30-31 18.586848072562358 28.341950113378683 35.16462585034014 17.906575963718822 32-33 18.446938775510205 28.934467120181406 34.64126984126984 17.97732426303855 34-35 18.71950113378685 28.976190476190478 34.5655328798186 17.738775510204082 36-37 19.07687074829932 28.8140589569161 34.160770975056685 17.94829931972789 38-39 18.42562358276644 29.189795918367345 34.40748299319728 17.977097505668933 40-41 18.53174603174603 29.204761904761906 34.25555555555555 18.00793650793651 42-43 18.678422888347644 29.431147637643495 33.59889150440631 18.291537969602548 44-45 18.829931972789115 29.723809523809525 33.07573696145125 18.37052154195011 46-47 19.153514739229024 29.979365079365078 32.22562358276644 18.641496598639456 48-49 19.124036281179137 30.291836734693877 31.62290249433107 18.96122448979592 50-51 19.23106575963719 30.66190476190476 30.822902494331068 19.284126984126985 52-53 19.41609977324263 30.88956916099773 30.531519274376418 19.16281179138322 54-55 18.96530612244898 30.820181405895692 30.54467120181406 19.66984126984127 56-57 19.239002267573696 30.88480725623583 30.413605442176873 19.462585034013603 58-59 19.307709750566893 30.186848072562356 30.660997732426303 19.844444444444445 60-61 18.981405895691612 30.4421768707483 30.89818594104308 19.678231292517008 62-63 19.019274376417233 29.897278911564623 31.271428571428572 19.81201814058957 64-65 18.9453514739229 29.8875283446712 31.0 20.167120181405895 66-67 18.910884353741498 30.07573696145125 30.65691609977324 20.356462585034013 68-69 18.887074829931976 29.470748299319727 30.75691609977324 20.885260770975055 70-71 18.782086167800454 29.641496598639456 30.66984126984127 20.906575963718822 72-73 18.88140589569161 29.322902494331064 30.549206349206347 21.246485260770974 74-75 18.420408163265307 29.49750566893424 30.09501133786848 21.987074829931974 76-77 18.186621315192745 29.00408163265306 30.933333333333334 21.875963718820863 78-79 18.181179138321994 28.73560090702948 30.77845804988662 22.304761904761904 80-81 17.719727891156463 29.24557823129252 30.46167800453515 22.573015873015873 82-83 17.728692031918584 28.968677791458898 30.17616899888208 23.12646117774044 84-85 17.29342403628118 28.87868480725624 30.194104308390024 23.633786848072564 86-87 17.130385487528343 28.774149659863944 30.387301587301586 23.708163265306123 88-89 16.960317460317462 28.207709750566895 30.17369614512472 24.658276643990927 90-91 16.994067327666755 28.594981721200686 29.790360768163136 24.62059018296942 92-93 16.98379332004508 28.695143438301464 29.49062452521707 24.83043871643639 94-95 16.555102040816326 28.373242630385487 29.115873015873017 25.95578231292517 96-97 16.959410430839004 28.675963718820864 28.382312925170066 25.98231292517007 98-99 17.365532879818595 29.236507936507937 27.57097505668934 25.826984126984126 100-101 17.357137511311294 28.5749924591376 27.33829102512638 26.729579004424732 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 497.0 1 322.5 2 190.5 3 256.0 4 251.5 5 271.5 6 404.5 7 704.5 8 1010.0 9 1166.0 10 1092.0 11 958.5 12 974.0 13 950.5 14 965.5 15 1027.0 16 1048.5 17 1063.0 18 1046.0 19 1097.5 20 1177.5 21 1275.0 22 1408.0 23 1565.0 24 1773.0 25 1986.0 26 2332.0 27 2728.5 28 2979.0 29 3298.0 30 3750.5 31 4269.5 32 4919.0 33 5492.5 34 6040.5 35 6576.0 36 7403.0 37 7851.5 38 7726.0 39 7697.5 40 7633.5 41 7711.0 42 7793.5 43 7937.0 44 7954.0 45 7885.5 46 7625.0 47 7136.5 48 6850.5 49 6519.5 50 6105.0 51 5585.5 52 5079.0 53 4678.5 54 4277.0 55 3848.5 56 3388.5 57 2947.5 58 2564.0 59 2242.5 60 1839.5 61 1439.5 62 1140.5 63 844.0 64 575.0 65 410.5 66 316.0 67 250.5 68 183.0 69 108.5 70 69.0 71 58.5 72 46.0 73 40.5 74 42.0 75 32.0 76 18.5 77 13.5 78 7.5 79 1.5 80 2.0 81 2.0 82 1.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04217687074829932 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0054421768707483 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.802721088435375E-4 24-25 0.0 26-27 0.0018140589569160996 28-29 0.003854875283446712 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009523809523809525 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.802721088435375E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.011791383219954649 92-93 0.0020408163265306124 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.015646258503401362 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 220500.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.78927039446747 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9018443910433 44.80778175745155 2 11.87126553284856 13.483210165389176 3 3.5419362882251226 6.034319327759789 4 1.6677797368843692 3.7884797774536105 5 0.936617859062853 2.659492242730379 6 0.6320151532818062 2.1535007659872782 7 0.45208872171182934 1.7971652061707564 8 0.33377511787638675 1.5163876336021904 9 0.24020966908692926 1.2277198664228863 >10 1.3387933013477804 13.393396391214168 >50 0.06004856407642531 2.3350896347049996 >100 0.01870365110577182 1.8347532154900186 >500 9.844026897774642E-4 0.302437991950061 >1k 0.003937610759109857 4.666266023673122 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2902 1.3160997732426305 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2474 1.1219954648526078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1879 0.8521541950113379 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1092 0.4952380952380952 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.24535147392290246 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 439 0.19909297052154196 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 336 0.1523809523809524 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 248 0.11247165532879819 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.5351473922902497E-4 0.0 18-19 0.0 0.0 0.0 4.5351473922902497E-4 0.0 20-21 0.0 0.0 0.0 4.5351473922902497E-4 0.0 22-23 0.0 0.0 0.0 0.0015873015873015873 0.0 24-25 0.0 0.0 0.0 0.0027210884353741495 0.0 26-27 0.0 0.0 0.0 0.0058956916099773245 0.0 28-29 0.0 0.0 0.0 0.011791383219954649 0.0 30-31 0.0 0.0 0.0 0.029705215419501135 0.0 32-33 0.0 0.0 0.0 0.05782312925170068 0.0 34-35 0.0 0.0 0.0 0.09931972789115645 0.0 36-37 0.0 0.0 0.0 0.1709750566893424 0.0 38-39 0.0 0.0 0.0 0.2841269841269841 0.0 40-41 0.0 0.0 0.0 0.42108843537414964 0.0 42-43 0.0 0.0 0.0 0.5839002267573696 0.0 44-45 0.0 0.0 0.0 0.7857142857142857 0.0 46-47 0.0 0.0 0.0 1.0537414965986396 0.0 48-49 0.0 0.0 0.0 1.3591836734693876 0.0 50-51 0.0 0.0 0.0 1.7231292517006802 0.0 52-53 0.0 0.0 0.0 2.0904761904761906 0.0 54-55 0.0 0.0 0.0 2.4959183673469387 0.0 56-57 0.0 0.0 0.0 2.9961451247165534 0.0 58-59 0.0 0.0 0.0 3.533333333333333 0.0 60-61 0.0 0.0 0.0 4.122902494331066 0.0 62-63 0.0 0.0 0.0 4.804308390022676 0.0 64-65 0.0 0.0 0.0 5.486167800453515 0.0 66-67 0.0 0.0 0.0 6.269387755102041 0.0 68-69 0.0 0.0 0.0 7.216326530612244 0.0 70-71 0.0 0.0 0.0 8.455555555555556 0.0 72-73 0.0 0.0 0.0 9.658503401360544 0.0 74-75 0.0 0.0 0.0 10.80090702947846 0.0 76-77 0.0 0.0 0.0 12.194104308390022 0.0 78-79 0.0 0.0 0.0 13.668934240362812 0.0 80-81 0.0 0.0 0.0 15.160997732426303 0.0 82-83 0.0 0.0 0.0 16.590249433106578 0.0 84-85 0.0 0.0 0.0 18.031972789115645 0.0 86-87 0.0 0.0 0.0 19.452380952380953 0.0 88-89 0.0 0.0 0.0 21.208843537414968 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCACCG 20 0.0020733592 71.2369 5 GGTATCA 970 0.0 63.676975 1 GTATCAA 1860 0.0 44.447475 1 ACCGTCT 55 0.002571718 34.55478 8 TATCAAC 2570 0.0 32.338413 2 ATCAACG 2635 0.0 31.720924 3 GTCTAAG 75 3.0655842E-4 31.675215 1 AGGTGTC 60 0.0039388807 31.675213 7 CATATAG 60 0.003947641 31.660849 3 TAGGACC 90 2.4072162E-5 31.660847 4 TCAACGC 2670 0.0 31.305105 4 CAACGCA 2700 0.0 30.957272 5 AACGCAG 2765 0.0 30.401283 6 GTGGTAT 385 0.0 29.61838 1 GAGTTAC 65 0.005826276 29.238657 1 TGGTATC 390 0.0 29.225395 2 TCTATAG 85 6.3975167E-4 27.936039 3 ACGCAGA 3095 0.0 26.865082 7 ATAGGAT 160 6.204937E-8 26.713837 6 CGCAGAG 3080 0.0 26.687393 8 >>END_MODULE