FastQCFastQC Report
Thu 26 May 2016
SRR937170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193741
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37501.9355737814917853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27701.429743833261932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.9951430001909767No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.3055625809715032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.28594876665238644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.24981805606453977No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.17807278789724426No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.16516896268729903No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2730.140909771292602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.13832900625061292No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.12387672201547427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11200.066.647471
GTATCAA20650.042.8247571
CTATATG550.002562488734.5779531
GTATCAT550.002562488734.5779531
GCACCAT702.0481844E-433.925346
CGACCCG851.6176456E-533.5522655
TATCAAC26850.032.9274562
ATCAACG26900.032.8662533
CAACGCA27300.032.2105835
TCAACGC27300.032.2105834
AACGCAG27800.031.7775466
GTCTTGG1051.8770261E-631.696461
TATATAG902.3914896E-531.688255
TGGTATC3350.029.7964152
GCTTACA804.4621146E-429.7077352
TAAGCCA650.00581280729.2506944
ACGCAGA30500.028.8087277
GAGTACT21150.028.74430712-13
CGCAGAG30450.028.7000548
GCAGAGT30650.028.3578199
Produced by FastQC (version 0.11.5)