##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937170_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 193741 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.137709622640536 33.0 31.0 34.0 31.0 34.0 2 32.25475764035491 33.0 31.0 34.0 31.0 34.0 3 32.24121378541455 34.0 31.0 34.0 31.0 34.0 4 35.884221718686284 37.0 35.0 37.0 35.0 37.0 5 35.909559669868536 37.0 35.0 37.0 35.0 37.0 6 35.990012439287504 37.0 37.0 37.0 35.0 37.0 7 35.953195245198486 37.0 37.0 37.0 35.0 37.0 8 35.95851162118498 37.0 37.0 37.0 35.0 37.0 9 37.639704554017996 39.0 38.0 39.0 35.0 39.0 10-11 37.57501251671045 39.0 38.0 39.0 35.0 39.0 12-13 37.45830515998162 39.0 37.0 39.0 35.0 39.0 14-15 38.61966233270191 40.0 38.0 41.0 35.5 41.0 16-17 38.63382040972226 40.0 38.0 41.0 35.5 41.0 18-19 38.741951884216554 40.0 38.5 41.0 36.0 41.0 20-21 38.75327111969072 40.0 38.5 41.0 35.0 41.0 22-23 38.6275904429109 40.0 38.0 41.0 35.0 41.0 24-25 38.49193252847874 40.0 38.0 41.0 35.0 41.0 26-27 38.32746295311782 40.0 38.0 41.0 34.5 41.0 28-29 38.28464289954114 40.0 38.0 41.0 35.0 41.0 30-31 37.953713978971926 40.0 37.5 41.0 34.5 41.0 32-33 37.5852736385174 40.0 36.5 41.0 34.0 41.0 34-35 37.38281004020832 40.0 36.0 41.0 33.5 41.0 36-37 37.3060296994441 40.0 36.0 41.0 33.0 41.0 38-39 37.14481704956617 40.0 35.0 41.0 33.0 41.0 40-41 36.86069804532856 40.0 35.0 41.0 33.0 41.0 42-43 36.47303874760634 40.0 35.0 41.0 31.5 41.0 44-45 35.98072684666643 40.0 35.0 41.0 30.5 41.0 46-47 35.28493194522584 39.0 35.0 41.0 25.5 41.0 48-49 34.672673827429406 39.0 35.0 41.0 17.5 41.0 50-51 33.66684387919955 38.0 34.0 40.0 9.0 40.5 52-53 33.640723956209584 38.5 34.5 40.0 4.0 40.5 54-55 33.87057721390929 39.0 35.0 40.5 2.0 41.0 56-57 33.66915366391213 39.0 35.0 41.0 2.0 41.0 58-59 33.41145395140936 38.5 34.5 40.0 2.0 41.0 60-61 33.122604404849774 38.0 34.0 40.0 2.0 41.0 62-63 32.80313150030195 38.0 34.0 40.0 2.0 41.0 64-65 32.411015221352216 37.5 33.5 40.0 2.0 41.0 66-67 31.99390423297082 37.0 33.0 40.0 2.0 41.0 68-69 31.51758275223107 36.0 32.5 39.5 2.0 41.0 70-71 31.0625138716121 36.0 32.0 39.0 2.0 41.0 72-73 30.61249038665022 35.0 31.5 39.0 2.0 40.0 74-75 30.14569709044549 35.0 31.0 38.0 2.0 40.0 76-77 29.706848318115423 35.0 30.5 37.0 2.0 39.5 78-79 29.24015309098229 35.0 30.0 37.0 2.0 39.0 80-81 28.76085856891417 35.0 29.0 36.0 2.0 39.0 82-83 28.27201779695573 34.5 29.0 36.0 2.0 37.5 84-85 27.80884015257483 34.0 27.5 35.0 2.0 37.0 86-87 27.3643575701581 34.0 26.5 35.0 2.0 36.5 88-89 26.942629593116585 34.0 25.5 35.0 2.0 36.0 90-91 26.512083141926592 34.0 25.0 35.0 2.0 36.0 92-93 26.082643838939617 34.0 23.5 35.0 2.0 35.0 94-95 25.54559437599682 33.5 19.0 35.0 2.0 35.0 96-97 24.9681069056111 33.0 7.5 35.0 2.0 35.0 98-99 24.282456991550575 32.5 2.0 35.0 2.0 35.0 100-101 22.654962036946234 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24177215877336522 1101 2 0.23917153683860448 1101 3 0.22640802668372118 1101 4 0.11384208793678852 1101 5 0.15426566711726508 1101 6 0.3194895791851664 1101 7 0.20358965875772128 1101 8 0.33021396668374337 1101 9 0.44038232949638 1101 10-11 0.356706796690176 1101 12-13 0.3901022865672914 1101 14-15 0.4890230671207618 1101 16-17 0.5607095447328803 1101 18-19 0.49608554912270364 1101 20-21 0.4087932098903977 1101 22-23 0.362737947805293 1101 24-25 0.41863148937667205 1101 26-27 0.574379171147605 1101 28-29 0.5647112818891387 1101 30-31 0.621668036583479 1101 32-33 0.5403064726560913 1101 34-35 0.7362315260433263 1101 36-37 1.0480021842216587 1101 38-39 1.0671228038312748 1101 40-41 1.1445745574006665 1101 42-43 1.3206167873670864 1101 44-45 1.5092841163463362 1101 46-47 1.5287865312044673 1101 48-49 1.8577570776523586 1101 50-51 1.8501497591240295 1101 52-53 1.6398215500279676 1101 54-55 1.8158308378065016 1101 56-57 1.8876866637885428 1101 58-59 2.1186219187366575 1101 60-61 2.2083692068046474 1101 62-63 2.200926048947256 1101 64-65 2.320489526890068 1101 66-67 2.329288751225885 1101 68-69 2.438142520453834 1101 70-71 2.4583206762422343 1101 72-73 2.467062665012879 1101 74-75 2.573626177331267 1101 76-77 2.8257556405329503 1101 78-79 2.827283370953129 1101 80-81 2.7704401978441098 1101 82-83 2.7274206271212265 1101 84-85 3.08120521754644 1101 86-87 3.082257477806536 1101 88-89 2.9541714357926985 1101 90-91 3.267551215519674 1101 92-93 3.2868295443724804 1101 94-95 3.151367498025383 1101 96-97 3.1337907440749646 1101 98-99 3.420052867315185 1101 100-101 3.716290813282747 1102 1 0.15419060992080347 1102 2 0.14170434825927458 1102 3 0.05069231557772724 1102 4 0.11142440430796086 1102 5 -0.1481366661393153 1102 6 -0.052795323723728416 1102 7 0.03555680896111113 1102 8 0.05477456227819033 1102 9 0.055011234247324126 1102 10-11 0.16155367672701715 1102 12-13 0.09602369266138311 1102 14-15 0.3311368133791106 1102 16-17 0.36266287960125254 1102 18-19 0.11507395959164768 1102 20-21 0.1138549951386878 1102 22-23 0.2595987966808835 1102 24-25 0.2857279664662471 1102 26-27 0.2643937540012331 1102 28-29 0.3364128706526657 1102 30-31 0.5396932111939634 1102 32-33 0.4666592242103178 1102 34-35 0.4177880537891241 1102 36-37 0.5881357324602021 1102 38-39 0.6214861471423561 1102 40-41 0.6284221282042566 1102 42-43 0.5103550635202794 1102 44-45 0.2604852829746278 1102 46-47 0.3817952041647956 1102 48-49 0.7233492182617667 1102 50-51 0.8222619703451528 1102 52-53 0.8299013719636505 1102 54-55 1.068110368085108 1102 56-57 1.3255675448999042 1102 58-59 1.4995222180689183 1102 60-61 1.5346989328005023 1102 62-63 1.3761467105483014 1102 64-65 1.6976136346496808 1102 66-67 1.6667321467282257 1102 68-69 1.1351683858310686 1102 70-71 1.219534123168323 1102 72-73 1.5112909250481863 1102 74-75 1.4705484276958387 1102 76-77 1.7772983529437276 1102 78-79 1.6466517031351806 1102 80-81 1.8287677747878526 1102 82-83 2.2698030048938875 1102 84-85 2.050611925659112 1102 86-87 2.0260981409426186 1102 88-89 2.295291245448084 1102 90-91 2.3499098002153502 1102 92-93 2.4462009466289842 1102 94-95 2.2742894413825105 1102 96-97 2.3082246990983712 1102 98-99 2.6913668591794853 1102 100-101 2.8459630828352864 1103 1 0.019379112015272426 1103 2 0.038107641629203215 1103 3 0.16354801397437768 1103 4 0.16559167283316611 1103 5 0.09128266074209535 1103 6 0.16358485827829838 1103 7 -0.05932980089010442 1103 8 -0.1549755460563489 1103 9 -0.16685789763192105 1103 10-11 0.23548116481021708 1103 12-13 0.23914806070991546 1103 14-15 0.10525208132652608 1103 16-17 -0.007956793950675944 1103 18-19 0.02839188534638737 1103 20-21 -0.18514832712045148 1103 22-23 -0.2070558328149943 1103 24-25 -0.051127443038303966 1103 26-27 -0.05293977192683741 1103 28-29 0.06337372577328182 1103 30-31 -0.25369838204596107 1103 32-33 -0.39736648718648127 1103 34-35 -0.502622172991174 1103 36-37 -0.29302850269796465 1103 38-39 -0.5440634700756206 1103 40-41 -0.38892584232875294 1103 42-43 -0.5318881836269114 1103 44-45 -0.23562189599402927 1103 46-47 -0.07991937044159414 1103 48-49 0.3057051333274927 1103 50-51 0.41170296413397267 1103 52-53 0.5357586592668397 1103 54-55 0.42710935696275953 1103 56-57 0.7035395223663201 1103 58-59 0.8451455027720769 1103 60-61 1.0903106618197143 1103 62-63 0.9173282792323967 1103 64-65 1.0584976444720091 1103 66-67 1.068747169117362 1103 68-69 1.2141659302482317 1103 70-71 1.4163490877790395 1103 72-73 1.2136598402583552 1103 74-75 1.0355852614384347 1103 76-77 1.647614689791915 1103 78-79 1.57430589929292 1103 80-81 1.3882578802334677 1103 82-83 1.5190591077394764 1103 84-85 1.5988496838564217 1103 86-87 1.4541134522238544 1103 88-89 1.5443667907319174 1103 90-91 1.6472736598137914 1103 92-93 1.5314024488678974 1103 94-95 1.4061181283679218 1103 96-97 1.4088097055984434 1103 98-99 2.1573281476382498 1103 100-101 2.4444030655017563 1104 1 -0.17794003303405148 1104 2 -0.21186952314975116 1104 3 -0.1908774518641323 1104 4 -0.1399435847481243 1104 5 -0.021683177767222617 1104 6 -0.2398678271436978 1104 7 -0.1920252703822527 1104 8 -0.06468508204465451 1104 9 -0.1495031296377647 1104 10-11 -0.0433368841484949 1104 12-13 -0.11283403647677659 1104 14-15 -0.057551627113369364 1104 16-17 -0.026282058839235845 1104 18-19 0.03391800883925811 1104 20-21 0.023097471198880726 1104 22-23 0.09768019202972056 1104 24-25 -0.19482948907410957 1104 26-27 -0.18899201891257889 1104 28-29 0.041269231801784656 1104 30-31 -0.25509133052970867 1104 32-33 -0.1779885186077408 1104 34-35 0.2630770414772243 1104 36-37 0.2821678802030192 1104 38-39 0.5203661061026779 1104 40-41 0.8391180926365394 1104 42-43 1.455815049840389 1104 44-45 1.293949715259167 1104 46-47 1.2333341918528902 1104 48-49 1.489448739465601 1104 50-51 1.2222619703451514 1104 52-53 1.2706759957666591 1104 54-55 1.35989388381698 1104 56-57 1.8895552329983971 1104 58-59 2.2729062809964162 1104 60-61 2.0975836797225256 1104 62-63 2.498598830931332 1104 64-65 2.2000760149506355 1104 66-67 2.265381257535342 1104 68-69 2.264426251768139 1104 70-71 2.3015792668071704 1104 72-73 2.3346065885228775 1104 74-75 2.1309451445221015 1104 76-77 2.170415658005286 1104 78-79 2.4855111422596714 1104 80-81 2.8002281031052227 1104 82-83 2.2029168899828058 1104 84-85 1.9983632936481683 1104 86-87 2.185708264061631 1104 88-89 2.4421106708926814 1104 90-91 2.4119224541141193 1104 92-93 2.374227622415578 1104 94-95 2.569629728660214 1104 96-97 2.8531716895224477 1104 98-99 2.838400032914098 1104 100-101 2.7539230691553946 1105 1 0.20313233973528355 1105 2 0.2231519573525631 1105 3 0.15252142454036033 1105 4 0.4917484178956286 1105 5 0.40177187841255346 1105 6 0.31185061304136497 1105 7 0.26051108123704836 1105 8 0.3023836555627426 1105 9 0.21401306335628334 1105 10-11 -0.05168123312404305 1105 12-13 0.061571118843772865 1105 14-15 -0.03055641891772609 1105 16-17 0.11757534367232836 1105 18-19 0.427093818488963 1105 20-21 0.48379228283139497 1105 22-23 0.22900041674882488 1105 24-25 0.2374656199807461 1105 26-27 0.5681956876307126 1105 28-29 0.49665588084341294 1105 30-31 0.410845549840424 1105 32-33 0.4357342176777834 1105 34-35 0.45691792052546987 1105 36-37 0.7545323878038204 1105 38-39 0.4152149164133263 1105 40-41 1.0111893087634414 1105 42-43 1.2374781364233343 1105 44-45 1.2731714601368935 1105 46-47 1.043396981013494 1105 48-49 0.8322073316665239 1105 50-51 1.1801271388844796 1105 52-53 1.193201607134803 1105 54-55 1.6199525421117613 1105 56-57 1.8020634947300636 1105 58-59 1.9771156332766466 1105 60-61 1.8668128601587455 1105 62-63 1.9731940060800461 1105 64-65 1.9371877135626683 1105 66-67 2.1005184022808727 1105 68-69 2.142772253090019 1105 70-71 2.540921632967123 1105 72-73 2.264361215092606 1105 74-75 1.8143555873509207 1105 76-77 1.5126785463066774 1105 78-79 1.7176864562054739 1105 80-81 1.348600541968409 1105 82-83 1.1898840082908038 1105 84-85 1.201787781420805 1105 86-87 1.4881702600960018 1105 88-89 0.7951344646798546 1105 90-91 1.1256741064604512 1105 92-93 0.49216384184716944 1105 94-95 0.6041431126655006 1105 96-97 0.8320423108046704 1105 98-99 1.2021847321870673 1105 100-101 0.900052447873172 1106 1 0.3825174833058185 1106 2 0.12881675136005555 1106 3 -0.26032189630857516 1106 4 -0.0639631925912596 1106 5 -0.014738733322779751 1106 6 0.015034133640611458 1106 7 0.1703113309249389 1106 8 0.08523321860893418 1106 9 0.02900994225765885 1106 10-11 -0.23247087107660036 1106 12-13 -0.23926377503887153 1106 14-15 -0.18867907809375595 1106 16-17 -0.5936840196235522 1106 18-19 -0.21792022645485787 1106 20-21 -0.053699914422033146 1106 22-23 0.09073574758527769 1106 24-25 0.06436168739647741 1106 26-27 0.22787889612009593 1106 28-29 -0.0034611603550800396 1106 30-31 0.3519348132611455 1106 32-33 0.46518958596742266 1106 34-35 0.5335018780785248 1106 36-37 0.2697087298762284 1106 38-39 0.3187729688477745 1106 40-41 -0.19601262631771021 1106 42-43 -0.5627715514668026 1106 44-45 -0.48607479454474856 1106 46-47 -0.1872131937680237 1106 48-49 0.08197324926951666 1106 50-51 0.21878974812292995 1106 52-53 0.03558612648561166 1106 54-55 -0.49876624690197247 1106 56-57 -0.8383859434721899 1106 58-59 -0.3492342418794081 1106 60-61 -0.44592122223825825 1106 62-63 -0.3843668553431776 1106 64-65 -0.2846053575983767 1106 66-67 -0.3385811607653153 1106 68-69 -0.49272244104231433 1106 70-71 -0.19249753057844643 1106 72-73 -0.05734602585620863 1106 74-75 -0.3958113914256103 1106 76-77 0.043577422711170044 1106 78-79 -0.4293172891128769 1106 80-81 -0.5453601321888932 1106 82-83 -0.9594605609975879 1106 84-85 -0.8304765756328791 1106 86-87 -1.008123435284773 1106 88-89 -0.8732488062315049 1106 90-91 -1.444082447846661 1106 92-93 -1.5659276063425907 1106 94-95 -1.6719960883332519 1106 96-97 -1.7949083758370286 1106 98-99 -1.4085362415957086 1106 100-101 -1.0598024210406827 1107 1 -0.40314458854046364 1107 2 -0.4502872586650106 1107 3 -0.15845263983405644 1107 4 -0.2610183304358742 1107 5 -0.24771701235368937 1107 6 -0.43546711949221617 1107 7 -0.5416769731686273 1107 8 -0.46765942967844865 1107 9 -0.4560046776253799 1107 10-11 -0.17707195712505808 1107 12-13 -0.3523851200681136 1107 14-15 -0.34759198536193026 1107 16-17 -0.5259365258892217 1107 18-19 -0.6744521813420761 1107 20-21 -0.42387013163172327 1107 22-23 0.12540575593948944 1107 24-25 -0.26435071861855874 1107 26-27 0.0238009930290346 1107 28-29 -0.21923496987889024 1107 30-31 -0.326271118234267 1107 32-33 -0.050030756555550226 1107 34-35 -0.17449206511277993 1107 36-37 -0.13006675049135907 1107 38-39 0.23919903401068865 1107 40-41 0.24224447476833433 1107 42-43 0.4950814142809037 1107 44-45 1.0786965087133922 1107 46-47 0.19590606270649147 1107 48-49 -0.014987903612684761 1107 50-51 -0.08036960860221853 1107 52-53 -0.18768037309667562 1107 54-55 0.004340478244230894 1107 56-57 -0.37973932693083157 1107 58-59 -0.010444559339724435 1107 60-61 0.4876627376614877 1107 62-63 0.3683743727270006 1107 64-65 0.2789128128276879 1107 66-67 0.7070276529356043 1107 68-69 0.05962196163103428 1107 70-71 -0.36388955229941544 1107 72-73 -0.11692204924802141 1107 74-75 0.07742900122267926 1107 76-77 0.015381933989363716 1107 78-79 -0.014530321694333992 1107 80-81 -0.15391276376783836 1107 82-83 -0.4090219645063584 1107 84-85 0.03624752628859085 1107 86-87 -0.22666457981275911 1107 88-89 -0.3142471353877312 1107 90-91 0.0938248203237606 1107 92-93 0.1215462867234045 1107 94-95 0.2732315641312404 1107 96-97 0.20253314463080585 1107 98-99 0.05590611429402159 1107 100-101 0.3096368020169571 1108 1 -0.042708614885395235 1108 2 0.028526053685631325 1108 3 -0.012098382096688454 1108 4 -0.05136629336645626 1108 5 0.03403387649634482 1108 6 0.16038297084991626 1108 7 0.07276611128669686 1108 8 -0.11421768578382796 1108 9 0.012375575332590927 1108 10-11 -0.33922151707142945 1108 12-13 -0.41521661741612803 1108 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0.37251448706225787 2316 70-71 0.9412851491601124 2316 72-73 0.7092389414640543 2316 74-75 0.2397686739338667 2316 76-77 0.031812716828817145 2316 78-79 -0.027356504799154635 2316 80-81 -0.3387424851300693 2316 82-83 0.10475512527692388 2316 84-85 0.3119662348246379 2316 86-87 -0.012821147703075297 2316 88-89 0.18181655324561774 2316 90-91 0.015231277643533048 2316 92-93 0.6381982106508737 2316 94-95 0.4413209051308016 2316 96-97 0.7697158071695078 2316 98-99 0.18582650350233365 2316 100-101 -0.993503401432843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 953.0 3 165.0 4 121.0 5 97.0 6 117.0 7 148.0 8 173.0 9 213.0 10 282.0 11 439.0 12 712.0 13 1140.0 14 1352.0 15 1971.0 16 2898.0 17 4613.0 18 3827.0 19 2028.0 20 1838.0 21 1653.0 22 1605.0 23 1737.0 24 1719.0 25 1842.0 26 1974.0 27 2087.0 28 2241.0 29 2565.0 30 3016.0 31 3835.0 32 4944.0 33 6788.0 34 10081.0 35 16288.0 36 23955.0 37 38096.0 38 39020.0 39 7206.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09812774418027 20.926186728522904 16.42780217049126 19.547883356805567 2 14.560237659017275 21.86649276982476 42.46188243331367 21.1113871378443 3 17.11797037543086 29.19914293699345 32.258381724269995 21.424504963305697 4 10.808726482233896 18.64599777438472 40.15631065448616 30.388965088895215 5 11.041236419724331 38.73180023084527 34.97668255668908 15.250280792741314 6 25.945179828577047 39.99524112495668 18.80479818748933 15.254780858976947 7 23.8459748118648 37.1434482401924 22.54001913677623 16.470557811166568 8 25.219046436810146 34.15727896223195 20.88880613226577 19.734868468692135 9 26.91858119640437 16.170492278092123 21.861326326895824 35.04960019860768 10-11 22.957226623626877 28.708107982277127 27.77049239690755 20.564172997188447 12-13 25.182719612680977 27.326733185398982 29.056944379787826 18.43360282213222 14-15 22.05001551670632 26.970104479155893 29.90948587979725 21.07039412434054 16-17 17.963379626037707 31.16171412315411 29.97594848320273 20.898957767605452 18-19 17.83704523590322 27.3189477713068 36.28983873136721 18.554168261422763 20-21 19.295119781538332 26.20376308494352 36.672752285986185 17.828364847531965 22-23 18.746799894493492 26.574193314817972 36.65316803980285 18.025838750885686 24-25 17.984009846459358 26.944598151720285 36.90793250210737 18.16345949971298 26-27 17.801049828045407 27.441884518915003 36.67778553512787 18.079280117911722 28-29 17.495804099956814 27.317701396736894 37.03305257223543 18.153441931070866 30-31 17.71914215660918 27.19271474534714 36.90526686047113 18.182876237572547 32-33 17.570538185015646 27.610882664804613 36.51563348591822 18.302945664261514 34-35 17.843573887427617 27.792907779407162 36.1790668021901 18.18445153097512 36-37 17.959240669340513 27.606546524647733 35.979767732325854 18.4544450736859 38-39 17.492222834224183 28.26078524161579 35.872969080109335 18.374022844050693 40-41 17.591615725944084 28.192974834765877 35.94682512592961 18.268584313360435 42-43 17.669831106329827 28.253679275290732 35.2119122921801 18.864577326199335 44-45 17.781013139418516 28.462964734845446 34.95431847509303 18.801703650643006 46-47 17.843612374674482 28.851569591381278 34.308611741096946 18.996206292847297 48-49 17.881323933317127 29.029705223629698 33.75290302224659 19.336067820806587 50-51 18.08675996306766 29.30120805994005 33.070737845044526 19.54129413194777 52-53 18.17911389785614 29.48974629583644 32.81599205363794 19.51514775266948 54-55 17.952133162294132 29.388138293470167 32.83616444591575 19.823564098319952 56-57 18.079351699100673 29.31058557044377 32.67413777945565 19.93592495099991 58-59 18.131818405136414 28.72088723628174 33.01485019225349 20.13244416632836 60-61 18.071762676892128 28.631896304302295 33.35927621933719 19.937064799468388 62-63 18.10045666823544 28.096215808064873 33.86326846196414 19.940059061735546 64-65 18.002042689627533 27.974505164901935 33.679039160170134 20.34441298530039 66-67 18.122889485305905 28.195183501657382 33.39616396986198 20.285763043174732 68-69 17.93284620197852 27.67754179659422 33.53914891636544 20.850463085061822 70-71 18.09185433426165 27.56897091184601 33.54433816627491 20.79483658761743 72-73 18.24300737725901 27.169154627659164 33.378929431203765 21.208908563878065 74-75 17.759923872372447 27.24536420501708 33.13982504092118 21.85488688168929 76-77 17.667532937693135 26.89189713354796 33.553650621258555 21.886919307500357 78-79 17.682865336329478 26.710867580853282 33.703283262523954 21.902983820293286 80-81 17.509742719789813 26.957918174083716 33.41229224797581 22.120046858150655 82-83 17.715656893444383 26.85830422224923 33.096652246875784 22.329386637430606 84-85 17.536089111598933 26.893789544177004 32.770438861179414 22.79968248304465 86-87 17.52900384994273 26.755421334732997 32.613332850689964 23.102241964634306 88-89 17.973010360939234 26.171261903961568 32.613816481831506 23.24191125326769 90-91 18.149307916872452 26.50938249067181 32.38012985770471 22.961179734751028 92-93 18.162002549470326 26.47079356788805 32.066958502586964 23.30024538005466 94-95 18.37569489334195 26.4672268907563 31.552165481577248 23.604912734324497 96-97 19.17576578696294 26.834540372686867 30.47996297435871 23.509730865991482 98-99 19.645646080489847 27.336243186496073 29.485276625673595 23.532834107340484 100-101 20.40350396367792 26.705829424814226 29.164188829305875 23.726477782201975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 484.0 1 307.5 2 196.0 3 254.0 4 190.5 5 184.0 6 367.0 7 821.5 8 1484.0 9 1733.0 10 1302.5 11 920.0 12 864.0 13 889.0 14 902.0 15 896.0 16 915.0 17 927.0 18 962.5 19 1011.0 20 1108.0 21 1210.5 22 1299.0 23 1444.5 24 1585.0 25 1788.5 26 2102.0 27 2424.5 28 2698.5 29 2979.5 30 3310.5 31 3756.5 32 4243.5 33 4753.5 34 5350.5 35 5865.5 36 6391.5 37 6860.5 38 6886.0 39 6704.5 40 6707.5 41 6742.0 42 6682.5 43 6752.0 44 6820.5 45 6708.5 46 6465.0 47 6184.5 48 5985.0 49 5661.0 50 5278.0 51 4836.0 52 4350.5 53 4071.5 54 3796.5 55 3370.5 56 2877.0 57 2406.0 58 2085.5 59 1785.5 60 1458.5 61 1190.5 62 922.5 63 657.0 64 471.0 65 343.5 66 249.5 67 204.5 68 150.0 69 88.5 70 68.0 71 55.5 72 40.5 73 35.5 74 24.5 75 27.0 76 25.5 77 9.0 78 4.5 79 4.0 80 1.0 81 1.5 82 2.5 83 1.5 84 0.5 85 0.5 86 0.5 87 0.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3122725700806747 2 0.2699480233920543 3 0.2699480233920543 4 0.27665801250122585 5 0.27769031851802145 6 0.21575195751028434 7 0.20439659132553256 8 0.2080096623843172 9 0.20388043831713476 10-11 0.22272002312365477 12-13 0.2126550394598975 14-15 0.2069773563675216 16-17 0.20955812140951063 18-19 0.20594505035072597 20-21 0.20078352026674787 22-23 0.20026736725835007 24-25 0.1920089191239851 26-27 0.19407353115757636 28-29 0.20517082083812924 30-31 0.21626811051868217 32-33 0.20955812140951063 34-35 0.2149777279976876 36-37 0.19949313774575336 38-39 0.19949313774575336 40-41 0.1956219901827698 42-43 0.20362236181293583 44-45 0.2020739027877424 46-47 0.20362236181293583 48-49 0.21059042742630624 50-51 0.213945421980892 52-53 0.22968808873702523 54-55 0.2131711924682953 56-57 0.19304122514078073 58-59 0.19433160766177526 60-61 0.18968623058619496 62-63 0.19820275522475883 64-65 0.19020238359459277 66-67 0.18787969505680266 68-69 0.18787969505680266 70-71 0.19639621969536647 72-73 0.19433160766177526 74-75 0.1969123727037643 76-77 0.1979446787205599 78-79 0.1969123727037643 80-81 0.2020739027877424 82-83 0.19226699562818403 84-85 0.18865392456939936 86-87 0.1865893125358081 88-89 0.1912346896113884 90-91 0.19381545465337746 92-93 0.18968623058619496 94-95 0.18891200107359826 96-97 0.1865893125358081 98-99 0.19355737814917853 100-101 0.1858150830232114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 193741.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.95303404461073 #Duplication Level Percentage of deduplicated Percentage of total 1 81.63105988971203 48.94029712665563 2 10.596883855416921 12.706306771011928 3 2.886477990252149 5.1915933965582 4 1.4114029831588981 3.3847156439996224 5 0.7914714317557254 2.3725556846693903 6 0.5395959470756359 1.9410248511215737 7 0.3835269280327253 1.6095522081359663 8 0.31793927685938994 1.5249139431735936 9 0.22156219022129833 1.1954992980002415 >10 1.1634471586670831 11.920415924132115 >50 0.03576779927252143 1.589825501528023 >100 0.01589679967667619 1.9085053978627893 >500 0.001987099959584524 0.6826302078497991 >1k 0.0029806499393767857 5.032164045301137 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3750 1.9355737814917853 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2770 1.429743833261932 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1928 0.9951430001909767 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.3055625809715032 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 554 0.28594876665238644 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 484 0.24981805606453977 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 345 0.17807278789724426 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 320 0.16516896268729903 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 273 0.140909771292602 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 268 0.13832900625061292 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 240 0.12387672201547427 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.002064612033591238 0.0 26-27 0.0 0.0 0.0 0.003871147562983571 0.0 28-29 0.0 0.0 0.0 0.011871519193149617 0.0 30-31 0.0 0.0 0.0 0.02787226245348171 0.0 32-33 0.0 0.0 0.0 0.053679912873372176 0.0 34-35 0.0 0.0 0.0 0.09781099509138488 0.0 36-37 0.0 0.0 0.0 0.1636205036621056 0.0 38-39 0.0 0.0 0.0 0.2733030179466401 0.0 40-41 0.0 0.0 0.0 0.4211808548526125 0.0 42-43 0.0 0.0 0.0 0.5780913694055465 0.0 44-45 0.0 0.0 0.0 0.7876494908150573 0.0 46-47 0.0 0.0 0.0 1.0666301918540733 0.0 48-49 0.0 0.0 0.0 1.3685797017667918 0.0 50-51 0.0 0.0 0.0 1.7489844689559773 0.0 52-53 0.0 0.0 0.0 2.1368734547669312 0.0 54-55 0.0 0.0 0.0 2.5608931511657316 0.0 56-57 0.0 0.0 0.0 3.097434203395254 0.0 58-59 0.0 0.0 0.0 3.673719037271409 0.0 60-61 0.0 0.0 0.0 4.283553816693421 0.0 62-63 0.0 0.0 0.0 5.000232268853779 0.0 64-65 0.0 0.0 0.0 5.7809136940554655 0.0 66-67 0.0 0.0 0.0 6.610887731559144 0.0 68-69 0.0 0.0 0.0 7.666420633732663 0.0 70-71 0.0 0.0 0.0 9.070098740070506 0.0 72-73 0.0 0.0 0.0 10.41983885703078 0.0 74-75 0.0 0.0 0.0 11.701446776882538 0.0 76-77 0.0 0.0 0.0 13.259970785739725 0.0 78-79 0.0 0.0 0.0 14.790622532143427 0.0 80-81 0.0 0.0 0.0 16.460377514310345 0.0 82-83 0.0 0.0 0.0 18.016578834629737 0.0 84-85 0.0 0.0 0.0 19.6042654884614 0.0 86-87 0.0 0.0 0.0 21.20150097294842 0.0 88-89 0.0 0.0 0.0 23.15668856875932 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1120 0.0 66.64747 1 GTATCAA 2065 0.0 42.824757 1 CTATATG 55 0.0025624887 34.577953 1 GTATCAT 55 0.0025624887 34.577953 1 GCACCAT 70 2.0481844E-4 33.92534 6 CGACCCG 85 1.6176456E-5 33.552265 5 TATCAAC 2685 0.0 32.927456 2 ATCAACG 2690 0.0 32.866253 3 CAACGCA 2730 0.0 32.210583 5 TCAACGC 2730 0.0 32.210583 4 AACGCAG 2780 0.0 31.777546 6 GTCTTGG 105 1.8770261E-6 31.69646 1 TATATAG 90 2.3914896E-5 31.68825 5 TGGTATC 335 0.0 29.796415 2 GCTTACA 80 4.4621146E-4 29.707735 2 TAAGCCA 65 0.005812807 29.250694 4 ACGCAGA 3050 0.0 28.808727 7 GAGTACT 2115 0.0 28.744307 12-13 CGCAGAG 3045 0.0 28.700054 8 GCAGAGT 3065 0.0 28.357819 9 >>END_MODULE