FastQCFastQC Report
Thu 26 May 2016
SRR937170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193741
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26841.3853546745397205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22721.172699635079823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16100.8310063435204733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10390.536282975725324No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.262721881274485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.22039733458586466No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.15174898446895596No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.11871519193149618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.068.444881
GTATCAA18450.041.4881941
GCTAGTG500.001611315938.005683
GTGGTAT3100.035.2745251
TGGTATC3300.034.5506172
AGAGTGC702.0483648E-433.9248858
GTATAGG851.6149766E-533.560421
CTACCCT753.0663834E-431.67144
TGCTAGT753.0663834E-431.67142
TATCAAC25250.030.4798032
CTAGGAC1102.7148362E-630.2317923
CTATACA953.4724864E-530.0044844
ATCAACG26250.029.3186683
TCAACGC26250.029.3186684
CACCGTG653.030982E-729.2275964-65
CAACGCA26850.028.6635025
TATCCAG1004.9271344E-528.504265
TAGGACA856.382228E-427.9453534
AACGCAG27550.027.9279986
CGAGCGC350.00838927227.13990892-93
Produced by FastQC (version 0.11.5)