##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937170_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 193741 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.442802504374395 31.0 31.0 34.0 30.0 34.0 2 31.674957804491562 31.0 31.0 34.0 30.0 34.0 3 31.65552980525547 31.0 31.0 34.0 30.0 34.0 4 35.53028527776774 37.0 35.0 37.0 33.0 37.0 5 35.34610639978115 37.0 35.0 37.0 33.0 37.0 6 35.47353941602449 37.0 35.0 37.0 33.0 37.0 7 35.4033064761718 37.0 35.0 37.0 33.0 37.0 8 35.39335504616989 37.0 35.0 37.0 33.0 37.0 9 36.912119788790186 39.0 37.0 39.0 33.0 39.0 10-11 36.808329161096516 39.0 37.0 39.0 32.0 39.0 12-13 36.691038551468196 39.0 35.0 39.0 32.0 39.0 14-15 37.547026700595126 39.0 36.0 41.0 32.0 41.0 16-17 37.53148275274722 39.0 36.5 40.0 32.0 41.0 18-19 37.69204763059962 39.0 37.0 40.5 32.0 41.0 20-21 37.65492590623565 39.0 36.5 41.0 32.0 41.0 22-23 37.49441006291905 39.0 36.0 40.5 32.0 41.0 24-25 37.30765558142056 39.0 36.0 40.0 32.0 41.0 26-27 36.989844689559774 39.0 36.0 40.0 31.0 41.0 28-29 36.85305640003923 39.0 35.5 40.0 31.0 41.0 30-31 36.759766905301404 38.5 35.0 40.0 31.0 41.0 32-33 36.521843595315396 38.0 35.0 40.0 31.0 41.0 34-35 36.26928992830635 38.0 35.0 40.0 30.0 41.0 36-37 35.953928182470406 38.0 35.0 40.0 30.0 41.0 38-39 35.69819501292963 38.0 35.0 40.0 30.0 41.0 40-41 35.37062882921013 38.0 35.0 40.0 28.5 41.0 42-43 34.951553878631785 38.0 34.0 40.0 26.5 41.0 44-45 34.31931547788027 38.0 33.5 40.0 24.0 41.0 46-47 33.59035516488508 38.0 33.0 40.0 20.0 41.0 48-49 32.95335009110101 37.5 33.0 40.0 11.5 41.0 50-51 32.594226312448065 37.0 32.5 40.0 7.5 41.0 52-53 32.228611909714516 37.0 31.5 40.0 2.0 41.0 54-55 31.927049514558096 37.0 31.0 40.0 2.0 41.0 56-57 31.58087085335577 36.0 31.0 39.5 2.0 40.5 58-59 31.231969485034142 36.0 30.5 39.0 2.0 40.0 60-61 30.807149751472323 35.5 29.5 39.0 2.0 40.0 62-63 30.340978419642717 35.0 29.0 39.0 2.0 40.0 64-65 29.906731151382516 35.0 28.0 38.0 2.0 40.0 66-67 29.408615109863167 34.5 28.0 38.0 2.0 40.0 68-69 28.86656670503404 34.0 26.5 38.0 2.0 40.0 70-71 28.424027438693926 34.0 26.0 37.0 2.0 39.0 72-73 27.94345801869506 34.0 26.0 36.5 2.0 39.0 74-75 27.47152125776165 33.0 25.0 36.0 2.0 39.0 76-77 26.347512916729038 31.5 23.5 34.5 2.0 37.0 78-79 26.908351871828884 33.0 25.0 35.0 2.0 37.5 80-81 26.69257152590314 33.0 24.5 35.0 2.0 37.0 82-83 26.287249472233547 33.0 23.5 35.0 2.0 36.5 84-85 25.865198899561786 32.5 21.5 35.0 2.0 36.0 86-87 25.392232413376618 32.0 20.0 35.0 2.0 36.0 88-89 24.907975080132754 32.0 18.0 34.0 2.0 35.0 90-91 24.284841618449374 31.0 10.0 34.0 2.0 35.0 92-93 23.66204623698649 31.0 4.5 34.0 2.0 35.0 94-95 23.01582525123748 31.0 2.0 34.0 2.0 35.0 96-97 22.358545687283538 30.0 2.0 34.0 2.0 35.0 98-99 21.402514181303907 29.5 2.0 34.0 2.0 35.0 100-101 19.7959621350153 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3245559910094258 1101 2 0.40094610464045033 1101 3 0.45385654493437144 1101 4 0.2850301561917874 1101 5 -0.05268296688790031 1101 6 5.136230119404672E-4 1101 7 0.043641917504970706 1101 8 0.1006199875313385 1101 9 -0.05519445788477384 1101 10-11 0.15014984050684177 1101 12-13 0.1943104521721324 1101 14-15 0.3045302343559797 1101 16-17 0.5004729170408808 1101 18-19 0.3014609851694985 1101 20-21 0.15836235248202257 1101 22-23 0.21408007764130588 1101 24-25 0.36570295568478883 1101 26-27 0.2873467830619205 1101 28-29 0.4720844463809186 1101 30-31 0.5594945493602239 1101 32-33 0.7183068127170813 1101 34-35 0.7632071840245445 1101 36-37 1.0387813751127268 1101 38-39 0.8618075308074751 1101 40-41 1.0969196828290393 1101 42-43 0.7497445118945478 1101 44-45 0.9721258653268379 1101 46-47 1.3427236751626523 1101 48-49 1.704511086266372 1101 50-51 1.5774098275750603 1101 52-53 1.9058870524758866 1101 54-55 1.9124464417887168 1101 56-57 2.0836748864290584 1101 58-59 1.929147628866339 1101 60-61 1.9350672085245257 1101 62-63 2.148689775645707 1101 64-65 2.1284094925463144 1101 66-67 2.030808798537503 1101 68-69 2.2107284877266835 1101 70-71 2.086004810723427 1101 72-73 1.7237272329729194 1101 74-75 2.2351404025840402 1101 76-77 2.357140682855075 1101 78-79 2.405531444766048 1101 80-81 2.1086559099768714 1101 82-83 1.9329283949322082 1101 84-85 1.877098893818765 1101 86-87 2.1362672208890707 1101 88-89 2.2941738366990734 1101 90-91 2.350351834417552 1101 92-93 2.327574378208407 1101 94-95 1.9577753969762348 1101 96-97 2.013875540453732 1101 98-99 2.1360497054776744 1101 100-101 2.2022085786939662 1102 1 0.11265945251836484 1102 2 0.04228465710034257 1102 3 0.11500475277840749 1102 4 0.1618127181689175 1102 5 0.004144111168741915 1102 6 -0.06746550048180922 1102 7 0.034324241551331625 1102 8 0.1796053279258416 1102 9 0.014465531369964424 1102 10-11 0.1314914630411934 1102 12-13 0.1145000292693723 1102 14-15 0.12174951442352722 1102 16-17 0.253612068165296 1102 18-19 0.17380728956737812 1102 20-21 0.09001865764742689 1102 22-23 0.17306772059165354 1102 24-25 0.24557478007653089 1102 26-27 -0.10350439507914899 1102 28-29 -0.030279510945049992 1102 30-31 0.03352939470522642 1102 32-33 -0.1459805462513728 1102 34-35 0.11827664445896602 1102 36-37 -0.015228909636682886 1102 38-39 -0.1394895756473531 1102 40-41 0.1423030583758944 1102 42-43 0.44992104117537934 1102 44-45 0.45798049730166923 1102 46-47 0.7653117479234126 1102 48-49 0.9483632621816369 1102 50-51 0.44203873232600444 1102 52-53 0.47261128303847855 1102 54-55 0.6433083651903573 1102 56-57 0.4427162556822637 1102 58-59 0.785928655806245 1102 60-61 0.8718658883925166 1102 62-63 1.1100145127705794 1102 64-65 1.2321204450369159 1102 66-67 1.1927475505522374 1102 68-69 1.3457158236695825 1102 70-71 1.3696681770600598 1102 72-73 1.2637878669432894 1102 74-75 0.9827112061760239 1102 76-77 1.4036254476106187 1102 78-79 1.2872184506759403 1102 80-81 1.1232886523208734 1102 82-83 0.7999097442299252 1102 84-85 0.8862860916083122 1102 86-87 1.0938080912551733 1102 88-89 0.9523440723272891 1102 90-91 0.9802236588092903 1102 92-93 1.0411748080188303 1102 94-95 1.1683057523373535 1102 96-97 1.023269968268604 1102 98-99 1.1183622390566121 1102 100-101 1.148129217269453 1103 1 0.03423969229880797 1103 2 0.053407002904187095 1103 3 -0.31012885981728644 1103 4 -0.0018013219870880448 1103 5 -0.1312451709343918 1103 6 -0.25403201788755325 1103 7 0.018622955981491884 1103 8 -0.1285992076801108 1103 9 -0.35144520097151144 1103 10-11 -0.08803764283776871 1103 12-13 -0.029158789161940035 1103 14-15 -0.47905216115064775 1103 16-17 -0.32414324022701635 1103 18-19 -0.2779621745377767 1103 20-21 -0.31693641963121166 1103 22-23 -0.30036142862112314 1103 24-25 -0.4411867394959117 1103 26-27 -0.2609794781356243 1103 28-29 -0.27213997606132523 1103 30-31 -0.2957931533230891 1103 32-33 -0.11079348861739646 1103 34-35 0.07307947560152428 1103 36-37 -0.249751515443414 1103 38-39 0.17782950567576705 1103 40-41 0.34653532762333583 1103 42-43 0.6135495259363282 1103 44-45 -0.35148071893407007 1103 46-47 -0.03752661694729653 1103 48-49 0.1642306607082915 1103 50-51 -0.18666269768988997 1103 52-53 -0.07042641738041766 1103 54-55 0.21617417481046886 1103 56-57 0.18009167096465362 1103 58-59 0.3236651862121356 1103 60-61 0.21689694429313633 1103 62-63 -0.11173906226914454 1103 64-65 0.32503536631381635 1103 66-67 1.0211384215619184 1103 68-69 1.3074015090660858 1103 70-71 1.1156309099793162 1103 72-73 1.0876142427919113 1103 74-75 0.7518214185117209 1103 76-77 0.7737612268970544 1103 78-79 1.3964196640227549 1103 80-81 1.3019250816145345 1103 82-83 0.950993089600427 1103 84-85 0.998462893572782 1103 86-87 1.6387885910385087 1103 88-89 1.9016651758951042 1103 90-91 1.8415987851995865 1103 92-93 1.3515171451559098 1103 94-95 1.1394165424572407 1103 96-97 1.1702004897188445 1103 98-99 0.9609016006050766 1103 100-101 1.114754957555455 1104 1 0.012043857443128303 1104 2 -0.11682956998583904 1104 3 -0.21659579441721277 1104 4 -1.5718607185277733E-4 1104 5 -0.4779523320597079 1104 6 -0.21274593823830656 1104 7 -0.10772432206015736 1104 8 0.06406155227604415 1104 9 -0.07799342895835792 1104 10-11 -0.02852905679465323 1104 12-13 -0.24422090096318527 1104 14-15 -0.17336847463256078 1104 16-17 -0.12417520953648165 1104 18-19 0.11518040858242529 1104 20-21 -0.21142628079306292 1104 22-23 -0.4183395981498066 1104 24-25 -0.248388968213483 1104 26-27 -0.23366855376588092 1104 28-29 -0.10486056429662227 1104 30-31 -0.40664673388574357 1104 32-33 -0.1134309566480951 1104 34-35 -0.04680543488439781 1104 36-37 0.3335057004864339 1104 38-39 0.025404405200795566 1104 40-41 0.2840010747917674 1104 42-43 0.15957391395239995 1104 44-45 0.5340919884097346 1104 46-47 0.6498363717264297 1104 48-49 0.33049390513649257 1104 50-51 0.4967155449115026 1104 52-53 0.32675799986474274 1104 54-55 0.5614769698415216 1104 56-57 0.12441940205708946 1104 58-59 0.2703677802932489 1104 60-61 0.5964982413336912 1104 62-63 0.6251991912931523 1104 64-65 0.746014083887804 1104 66-67 0.7200560320843863 1104 68-69 0.7021197224383897 1104 70-71 1.0009181770600613 1104 72-73 0.923645938078721 1104 74-75 0.887131520813508 1104 76-77 0.9755218224396174 1104 78-79 0.57376940826828 1104 80-81 1.40679583426342 1104 82-83 1.211794149154688 1104 84-85 1.2289844500214464 1104 86-87 0.7403368190253516 1104 88-89 0.9994798452411047 1104 90-91 1.388833439931041 1104 92-93 0.7726214564456448 1104 94-95 0.40530985630451966 1104 96-97 0.2198670954915869 1104 98-99 0.3998516371414276 1104 100-101 0.18159364955399226 1105 1 -0.5872621570975198 1105 2 -0.20477570349542162 1105 3 -0.07085884795323594 1105 4 -0.07186901687158809 1105 5 -0.6759447312665827 1105 6 -0.5759316619659955 1105 7 -0.5282051548427091 1105 8 -1.1007186856618247 1105 9 -0.41767452611632194 1105 10-11 -0.31760704754150737 1105 12-13 -0.490007417817111 1105 14-15 -0.35803883153937477 1105 16-17 -0.48938505752061445 1105 18-19 -0.37823086041955634 1105 20-21 -0.18203517041633432 1105 22-23 -0.29530175941880543 1105 24-25 -0.3086583006655701 1105 26-27 -0.4844947269317714 1105 28-29 -1.0558478412298626 1105 30-31 -1.5147721469723336 1105 32-33 -0.491838920957413 1105 34-35 -0.33373537539993237 1105 36-37 -0.11469324201191 1105 38-39 -0.43398918369543793 1105 40-41 -0.2540648538268755 1105 42-43 -0.2358119089160695 1105 44-45 0.220104876711126 1105 46-47 0.3241176010720963 1105 48-49 0.4325675997828924 1105 50-51 0.10665853896305322 1105 52-53 0.12990158876096913 1105 54-55 -0.05474494027083665 1105 56-57 0.657218476743143 1105 58-59 0.8543895912648267 1105 60-61 0.6269220681677936 1105 62-63 -0.12281799310869701 1105 64-65 0.6786190078798704 1105 66-67 0.4393636989713663 1105 68-69 0.8572130800061366 1105 70-71 0.604499637734218 1105 72-73 0.9976917080721108 1105 74-75 1.0240490687315535 1105 76-77 1.2833745983814566 1105 78-79 1.4447831227428338 1105 80-81 1.3258102096765043 1105 82-83 1.3280780222544912 1105 84-85 1.318582929862817 1105 86-87 1.6656920734866887 1105 88-89 1.1878679813944437 1105 90-91 1.4428445106514687 1105 92-93 1.2818332872453801 1105 94-95 1.090372645465127 1105 96-97 0.35507033409039934 1105 98-99 -0.7017056992762853 1105 100-101 -0.12289247073681864 1106 1 -0.5447371883085061 1106 2 -0.04411715168518526 1106 3 -0.1336709578159052 1106 4 -0.17907875469930445 1106 5 -0.9718248810793142 1106 6 -0.3839060689572591 1106 7 -0.24293674036081114 1106 8 -0.6352848529527151 1106 9 -0.6793006185008394 1106 10-11 -0.7313476842456268 1106 12-13 -0.20255423429652097 1106 14-15 -0.4828047491423604 1106 16-17 -0.1580804382946539 1106 18-19 -0.622768937822805 1106 20-21 -0.7163282415773864 1106 22-23 -0.6260601863850965 1106 24-25 -0.27514550416119476 1106 26-27 -0.6305231289292834 1106 28-29 -0.49088997606132523 1106 30-31 -0.6813607861733288 1106 32-33 -0.5529734403082216 1106 34-35 -0.37196883357722044 1106 36-37 -0.6840596589906838 1106 38-39 -1.0624223921848213 1106 40-41 -1.011750559195164 1106 42-43 -1.027517589315572 1106 44-45 -1.2351073579954956 1106 46-47 -1.258864608665732 1106 48-49 -1.2507969445367095 1106 50-51 -1.4416014485525537 1106 52-53 -0.41527631386075115 1106 54-55 -1.1989396968251427 1106 56-57 -0.4721900750670933 1106 58-59 -0.33236914781132754 1106 60-61 -0.3698007782741506 1106 62-63 0.002650171685310454 1106 64-65 -0.21619996839977773 1106 66-67 -0.6451400463469881 1106 68-69 -0.17778223834591955 1106 70-71 -0.2525540451621602 1106 72-73 -0.21442595734611558 1106 74-75 -0.9041266491211353 1106 76-77 -0.5888164128544986 1106 78-79 -0.2814185002284546 1106 80-81 -0.7241273683509633 1106 82-83 -0.17484800770806075 1106 84-85 -0.3451577068413023 1106 86-87 0.4978940085678367 1106 88-89 -0.1048910697915737 1106 90-91 0.6261700412382325 1106 92-93 0.10432080285087153 1106 94-95 -0.06384864042750849 1106 96-97 0.05973637653733732 1106 98-99 0.12023562406953303 1106 100-101 -0.3970419713610376 1107 1 -0.490278123981021 1107 2 -0.39494213079963814 1107 3 -0.0500765551425566 1107 4 -0.0872867747494297 1107 5 -0.06455671065324964 1107 6 -0.16298919343511642 1107 7 -0.5570553702689196 1107 8 -0.449455696728819 1107 9 -0.9518047956102791 1107 10-11 -0.884621493769437 1107 12-13 -0.7658374423165668 1107 14-15 -0.6010691601699278 1107 16-17 -0.2866309813188792 1107 18-19 0.404695556746951 1107 20-21 -0.009483003482145591 1107 22-23 -0.3633722164602844 1107 24-25 -0.3578261641444911 1107 26-27 -0.39443812475217044 1107 28-29 -0.9262753144071922 1107 30-31 -1.395907569799057 1107 32-33 -1.2914080685454792 1107 34-35 -0.8518142805279254 1107 36-37 -1.0447008452897677 1107 38-39 -1.2373388499960143 1107 40-41 -1.3663610437398646 1107 42-43 -1.5151690095327837 1107 44-45 -1.0253120363580663 1107 46-47 -0.7143000723248747 1107 48-49 -1.4762564265751372 1107 50-51 -1.441157630674521 1107 52-53 -0.9276510005775407 1107 54-55 -0.960844458729909 1107 56-57 -0.49284065986241643 1107 58-59 -1.1498555722056452 1107 60-61 -1.4484348634871829 1107 62-63 -0.6088786503698316 1107 64-65 -1.1332843460104733 1107 66-67 -0.832326763138969 1107 68-69 -0.41031669949880367 1107 70-71 -1.0322115221880601 1107 72-73 -0.754082390094652 1107 74-75 -0.8280249365062602 1107 76-77 -0.6837412248845745 1107 78-79 -1.025205049936055 1107 80-81 -0.8866169255773606 1107 82-83 -0.6575391104649491 1107 84-85 -0.543544298319997 1107 86-87 -0.884911964698663 1107 88-89 -0.8653338433922464 1107 90-91 -0.6100193906748856 1107 92-93 -0.546733917282797 1107 94-95 -1.097343836751648 1107 96-97 -0.338707650196163 1107 98-99 0.17714874019317506 1107 100-101 0.009573525714984044 1108 1 -0.1989684544583774 1108 2 -0.37244402507019814 1108 3 -0.28887121621383827 1108 4 -0.36997022756752074 1108 5 -0.6229876717769898 1108 6 -0.31010115939653815 1108 7 -0.5168385491463425 1108 8 0.27572935893359585 1108 9 -0.21886780196336986 1108 10-11 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1.2088376837268804 2316 98-99 0.9288957547884884 2316 100-101 0.20585835543634445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 3.0 6 22.0 7 55.0 8 157.0 9 285.0 10 435.0 11 779.0 12 1099.0 13 1528.0 14 1880.0 15 2793.0 16 3803.0 17 4820.0 18 3904.0 19 2420.0 20 2130.0 21 2255.0 22 2229.0 23 2469.0 24 2703.0 25 2968.0 26 3316.0 27 3964.0 28 4693.0 29 5551.0 30 6686.0 31 7858.0 32 9504.0 33 11902.0 34 15075.0 35 19786.0 36 25032.0 37 27662.0 38 13416.0 39 556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.8935772521441 23.430973145179195 17.520434138306715 18.15501546436999 2 15.896480352635736 24.53791401923186 39.25137167661982 20.314233951512588 3 18.194393546022784 29.464078331380556 32.967724952384884 19.37380317021178 4 12.394382190656598 21.176725628545327 38.23351794405934 28.19537423673874 5 11.262930767483637 40.48543288389668 34.1217402077182 14.129896140901488 6 26.536974620756578 41.06771411317171 17.243639704554017 15.151671561517697 7 23.916981950129294 38.156611145808064 21.52151583815506 16.404891065907577 8 26.078114596290924 35.66823749232223 19.78982249498041 18.46382541640644 9 27.044353028011624 16.820394237667816 21.169499486427757 34.965753247892806 10-11 23.632581642502103 29.76189861722609 26.788599212350512 19.816920527921297 12-13 25.332686371473113 28.317314144719862 28.294859750369078 18.055139733437947 14-15 22.271486159357078 28.43770807418151 28.68778420675025 20.60302155971116 16-17 18.516989176271416 32.239174981031375 29.190517236929715 20.053318605767494 18-19 18.50873072813705 28.693719966346826 34.766518186651254 18.031031118864878 20-21 19.962991829297877 27.28797724797539 35.52345657346664 17.225574349260096 22-23 19.465047977412677 27.862772727976587 35.30688510712977 17.365294187480966 24-25 18.81330955582338 28.40139258441059 35.382896193619814 17.40240166614621 26-27 18.510384056530974 28.839210362635466 35.34218221328674 17.308223367546823 28-29 18.082127436633968 28.811446534063194 35.56085487688111 17.54557115242173 30-31 18.330941787362313 28.578544219512953 35.4200002064644 17.670513786660337 32-33 18.16032744746853 29.157999597400654 34.95672057024584 17.724952384884975 34-35 18.45169582070909 29.13012733494717 34.89116913817932 17.527007706164415 36-37 18.712707752658204 28.87245793331269 34.53958914008465 17.875245173944464 38-39 18.19685711999876 29.40159337667332 34.64755509330828 17.75399441001964 40-41 18.168327819098693 29.5242101568589 34.37527420628571 17.932187817756695 42-43 18.455081845353323 29.683975242490412 33.87122584774854 17.989717064407724 44-45 18.5206022473302 30.0432020068029 33.19896150014711 18.2372342457198 46-47 18.91107199818314 30.47109285076468 32.37956859931558 18.238266551736597 48-49 18.924750052905683 30.74233125667773 31.620565600466605 18.712353089949985 50-51 19.042432938820383 30.870595279264585 31.017182733649562 19.069789048265466 52-53 19.19139261021831 31.2149498943174 30.627824332031768 18.96583316343253 54-55 18.883715888738056 31.20583665821896 30.599098796847336 19.311348656195644 56-57 18.985656107896627 31.221321248470897 30.33198961500147 19.461033028631007 58-59 19.01017337579552 30.61122839254469 30.703103628039496 19.675494603620297 60-61 19.069014818752873 30.507739714360927 30.90879060188602 19.51445486500018 62-63 18.875457440603693 30.133270706768318 31.355779107158526 19.635492745469467 64-65 18.64551127536247 30.09120423658389 31.363521402284494 19.899763085769145 66-67 18.691965046118273 30.103333832281244 31.045571149111446 20.159129972489044 68-69 18.60215442265705 29.803448934402116 30.752654316845685 20.841742326095147 70-71 18.670286619765562 29.88629149224996 30.748008939770106 20.69541294821437 72-73 18.74177381142866 29.501757500993598 30.661037157855077 21.09543152972267 74-75 18.17787664975405 29.57634161070708 30.3247634728839 21.92101826665497 76-77 17.788697281422106 29.22897063605535 31.094347608405037 21.88798447411751 78-79 17.82740875705194 28.995669476259543 30.97769702850713 22.199224738181385 80-81 17.42687402253524 29.31413588244099 30.66026292834248 22.59872716668129 82-83 17.22901827191081 29.082017136368325 30.44337772272117 23.24558686899969 84-85 16.865815702406824 29.06405974987225 30.35031304679959 23.719811500921335 86-87 16.78632813911356 28.841081650244398 30.27366432505252 24.098925885589527 88-89 16.692646368089356 28.279249100603383 30.286568150262465 24.741536381044796 90-91 16.748010705925168 28.682226564012296 29.976590467390547 24.59317226267199 92-93 16.704174753272493 28.72646281537763 29.55103852665483 25.018323904695052 94-95 16.137523807557514 28.62300700419632 29.00805714846109 26.231412039785074 96-97 16.46811980943631 29.157225367888056 28.129822804672216 26.244832018003418 98-99 17.014517065617138 29.57197238531518 27.4448674108007 25.968643138266984 100-101 16.99239620274728 28.84642187911356 26.976961475126345 27.18422044301282 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 490.0 1 314.0 2 166.0 3 208.5 4 215.5 5 236.0 6 357.0 7 654.5 8 936.5 9 1035.0 10 975.5 11 895.0 12 935.5 13 991.5 14 966.0 15 912.0 16 933.0 17 953.5 18 995.0 19 1075.5 20 1142.0 21 1203.0 22 1298.0 23 1422.5 24 1573.0 25 1793.0 26 2116.0 27 2473.5 28 2756.5 29 3070.5 30 3383.0 31 3799.0 32 4377.5 33 4911.0 34 5420.0 35 5827.5 36 6401.0 37 6793.0 38 6869.5 39 6817.5 40 6760.5 41 6813.5 42 6756.0 43 6891.0 44 6974.0 45 6868.0 46 6543.0 47 6229.5 48 6069.0 49 5608.5 50 5203.5 51 4798.0 52 4322.5 53 4056.5 54 3733.5 55 3268.0 56 2778.0 57 2419.5 58 2119.0 59 1860.5 60 1554.5 61 1213.0 62 988.0 63 738.0 64 492.0 65 345.0 66 253.5 67 198.5 68 128.5 69 72.5 70 47.0 71 34.0 72 35.5 73 28.5 74 19.0 75 20.5 76 18.5 77 15.0 78 9.0 79 3.0 80 1.5 81 3.0 82 3.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03613071058784666 2 0.0 3 0.0 4 0.0 5 0.008774601142762762 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007742295125967142 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002064612033591238 24-25 2.5807650419890473E-4 26-27 0.0033549945545857615 28-29 0.002838841546187952 30-31 0.0015484590251934284 32-33 0.0 34-35 0.0 36-37 5.161530083978095E-4 38-39 7.742295125967142E-4 40-41 0.0 42-43 0.011355366184751808 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.5807650419890473E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.161530083978095E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.008516524638563856 92-93 0.0025807650419890473 94-95 0.0 96-97 0.0 98-99 0.001806535529392333 100-101 0.011355366184751808 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 193741.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.56201438734481 #Duplication Level Percentage of deduplicated Percentage of total 1 81.68646946731893 49.470971391311714 2 10.40989012383782 12.608878309010896 3 3.04601093604286 5.5341767439791205 4 1.4085322495341193 3.41214201445298 5 0.8111497189126784 2.4562430473540164 6 0.5254939083350894 1.909498178223104 7 0.3913222652054927 1.658948525882439 8 0.2840443797862345 1.3761839852206696 9 0.24133591645929464 1.3154210320311766 >10 1.129210080715692 11.893484150435633 >50 0.04767113111770587 1.9914697419651437 >100 0.01390407990933088 1.492248997226071 >500 9.931485649522058E-4 0.3061486253881782 >1k 0.003972594259808823 4.574185257518852 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2684 1.3853546745397205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2272 1.172699635079823 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1610 0.8310063435204733 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1039 0.536282975725324 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 509 0.262721881274485 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 427 0.22039733458586466 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 294 0.15174898446895596 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 230 0.11871519193149618 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.002064612033591238 0.0 26-27 0.0 0.0 0.0 0.003871147562983571 0.0 28-29 0.0 0.0 0.0 0.011871519193149617 0.0 30-31 0.0 0.0 0.0 0.02787226245348171 0.0 32-33 0.0 0.0 0.0 0.053421836369173276 0.0 34-35 0.0 0.0 0.0 0.09729484208298708 0.0 36-37 0.0 0.0 0.0 0.1589751265865253 0.0 38-39 0.0 0.0 0.0 0.26581879932487185 0.0 40-41 0.0 0.0 0.0 0.41292240671824754 0.0 42-43 0.0 0.0 0.0 0.5685425387501871 0.0 44-45 0.0 0.0 0.0 0.7760360481261065 0.0 46-47 0.0 0.0 0.0 1.0524359841231334 0.0 48-49 0.0 0.0 0.0 1.348707810943476 0.0 50-51 0.0 0.0 0.0 1.723176818536087 0.0 52-53 0.0 0.0 0.0 2.1056461977588636 0.0 54-55 0.0 0.0 0.0 2.5255366700904816 0.0 56-57 0.0 0.0 0.0 3.0540773506898384 0.0 58-59 0.0 0.0 0.0 3.625200654482015 0.0 60-61 0.0 0.0 0.0 4.227293138778059 0.0 62-63 0.0 0.0 0.0 4.932616224753666 0.0 64-65 0.0 0.0 0.0 5.700909977753805 0.0 66-67 0.0 0.0 0.0 6.524690179156709 0.0 68-69 0.0 0.0 0.0 7.562673879044704 0.0 70-71 0.0 0.0 0.0 8.935898957887076 0.0 72-73 0.0 0.0 0.0 10.262412189469448 0.0 74-75 0.0 0.0 0.0 11.532664743136454 0.0 76-77 0.0 0.0 0.0 13.07234916718712 0.0 78-79 0.0 0.0 0.0 14.577193263170933 0.0 80-81 0.0 0.0 0.0 16.230689425573317 0.0 82-83 0.0 0.0 0.0 17.76185732498542 0.0 84-85 0.0 0.0 0.0 19.322962098884595 0.0 86-87 0.0 0.0 0.0 20.886647637825757 0.0 88-89 0.0 0.0 0.0 22.81628565972097 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1035 0.0 68.44488 1 GTATCAA 1845 0.0 41.488194 1 GCTAGTG 50 0.0016113159 38.00568 3 GTGGTAT 310 0.0 35.274525 1 TGGTATC 330 0.0 34.550617 2 AGAGTGC 70 2.0483648E-4 33.924885 8 GTATAGG 85 1.6149766E-5 33.56042 1 CTACCCT 75 3.0663834E-4 31.6714 4 TGCTAGT 75 3.0663834E-4 31.6714 2 TATCAAC 2525 0.0 30.479803 2 CTAGGAC 110 2.7148362E-6 30.231792 3 CTATACA 95 3.4724864E-5 30.004484 4 ATCAACG 2625 0.0 29.318668 3 TCAACGC 2625 0.0 29.318668 4 CACCGTG 65 3.030982E-7 29.22759 64-65 CAACGCA 2685 0.0 28.663502 5 TATCCAG 100 4.9271344E-5 28.50426 5 TAGGACA 85 6.382228E-4 27.945353 4 AACGCAG 2755 0.0 27.927998 6 CGAGCGC 35 0.008389272 27.139908 92-93 >>END_MODULE