FastQCFastQC Report
Thu 26 May 2016
SRR937169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224560
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43181.922871392946206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31341.39561809761311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22961.022443890274314No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6780.3019237620235126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.2854470965443534No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.2676344852155326No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.18658710366939793No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.16654791592447454No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.1505165657285358No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.1367117919486997No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2780.12379764873530459No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9000.066.593021
GTATCAA20700.051.932441
GTGGTAT2800.049.2652441
TGGTATC2900.047.5558052
TCCGGTG601.474873E-647.5558052
TATCAAC27000.039.805972
ATCAACG27350.039.470453
TCAACGC27800.038.8315354
CAACGCA28350.038.078195
AACCCGA500.001612589338.0021446
AACGCAG29000.037.1831326
ACTATAC550.002560416734.5860373
TAAGGAC550.002560416734.5860374
TAGACCC550.002574495334.5474059
TATGAGG702.0339867E-433.9684332
CGCAGAG32900.032.7754068
ACGCAGA33150.032.6715287
TTCCGGT902.3814771E-531.7109621
CTTACAC1201.4812758E-731.7038693
GCAGAGT33850.031.4345669
Produced by FastQC (version 0.11.5)