##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937169_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 224560 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14132080513003 33.0 31.0 34.0 31.0 34.0 2 32.24114267901675 33.0 31.0 34.0 31.0 34.0 3 32.24527965799786 34.0 31.0 34.0 31.0 34.0 4 35.89115603847524 37.0 35.0 37.0 35.0 37.0 5 35.89263448521553 37.0 35.0 37.0 35.0 37.0 6 35.99792037762736 37.0 37.0 37.0 35.0 37.0 7 35.967193623085144 37.0 36.0 37.0 35.0 37.0 8 35.97318756679729 37.0 37.0 37.0 35.0 37.0 9 37.6580334877093 39.0 38.0 39.0 35.0 39.0 10-11 37.604562255076594 39.0 38.0 39.0 35.0 39.0 12-13 37.51179862842893 39.0 37.5 39.0 35.0 39.0 14-15 38.682835322408266 40.0 38.0 41.0 35.5 41.0 16-17 38.65837860705379 40.0 38.0 41.0 35.5 41.0 18-19 38.753270840755256 40.0 38.5 41.0 36.0 41.0 20-21 38.76542349483434 40.0 38.0 41.0 35.0 41.0 22-23 38.6386756323477 40.0 38.0 41.0 35.0 41.0 24-25 38.5083251692198 40.0 38.0 41.0 35.0 41.0 26-27 38.34065505878162 40.0 38.0 41.0 34.5 41.0 28-29 38.130978357677236 40.0 38.0 41.0 34.0 41.0 30-31 37.895889739935875 40.0 37.5 41.0 34.0 41.0 32-33 37.57208763804773 40.0 36.5 41.0 34.0 41.0 34-35 37.32718649804062 40.0 35.5 41.0 33.5 41.0 36-37 37.241706002850016 40.0 35.5 41.0 33.0 41.0 38-39 37.1561809761311 40.0 35.0 41.0 33.0 41.0 40-41 36.896557712860705 40.0 35.0 41.0 33.0 41.0 42-43 36.52219228713929 40.0 35.0 41.0 31.5 41.0 44-45 36.039831225507655 40.0 35.0 41.0 30.5 41.0 46-47 35.38092937299608 39.5 35.0 41.0 26.0 41.0 48-49 34.81103713929462 39.0 35.0 41.0 21.5 41.0 50-51 33.765790879943 38.0 34.0 40.0 14.0 40.5 52-53 33.804168151050945 38.5 34.5 40.0 8.0 40.5 54-55 34.091641432133954 39.0 35.0 40.5 3.5 41.0 56-57 33.855118899180624 39.0 35.0 41.0 2.0 41.0 58-59 33.598076237976485 38.5 35.0 40.5 2.0 41.0 60-61 33.302930174563585 38.0 34.0 40.0 2.0 41.0 62-63 32.98047737798361 38.0 34.0 40.0 2.0 41.0 64-65 32.608781617385105 37.5 34.0 40.0 2.0 41.0 66-67 32.19806510509441 37.0 33.0 40.0 2.0 41.0 68-69 31.701082116138227 36.0 33.0 39.5 2.0 41.0 70-71 31.252117474171712 36.0 32.0 39.0 2.0 41.0 72-73 30.829515496971858 35.0 32.0 39.0 2.0 40.0 74-75 30.369854381902385 35.0 31.0 38.0 2.0 40.0 76-77 29.92369077306733 35.0 31.0 37.0 2.0 39.5 78-79 29.45678660491628 35.0 30.5 37.0 2.0 39.0 80-81 28.991062522265764 35.0 29.5 36.0 2.0 39.0 82-83 28.53274180619879 35.0 29.0 36.0 2.0 37.5 84-85 28.062758282864266 34.0 29.0 35.0 2.0 37.0 86-87 27.61992117919487 34.0 27.5 35.0 2.0 36.5 88-89 27.233830602066263 34.0 26.5 35.0 2.0 36.0 90-91 26.778598147488424 34.0 25.5 35.0 2.0 36.0 92-93 26.331995903099394 34.0 24.5 35.0 2.0 35.5 94-95 25.81461970074813 34.0 21.5 35.0 2.0 35.0 96-97 25.25699367652298 33.0 14.0 35.0 2.0 35.0 98-99 24.653949946562165 33.0 2.0 35.0 2.0 35.0 100-101 23.09036115069469 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.157397648325599 1101 2 0.22262928410464866 1101 3 0.3566363204738039 1101 4 0.23368295548520024 1101 5 0.2366564492065919 1101 6 0.024528212727581433 1101 7 0.07868207373228131 1101 8 0.024538798778671378 1101 9 0.1422041211022318 1101 10-11 0.2084075553851008 1101 12-13 0.3187930339187588 1101 14-15 0.38512652003763037 1101 16-17 0.47717303566765423 1101 18-19 0.43546328609139096 1101 20-21 0.4327302571050282 1101 22-23 0.24249801871044951 1101 24-25 0.37387285653404234 1101 26-27 0.37539056289205064 1101 28-29 0.22924924935134072 1101 30-31 0.4975932218901633 1101 32-33 0.6437557266076723 1101 34-35 0.40514602529970034 1101 36-37 0.5432643972843607 1101 38-39 0.697733344904222 1101 40-41 0.8826276900978343 1101 42-43 0.9574510395355134 1101 44-45 0.9240974612372739 1101 46-47 1.288866142061842 1101 48-49 1.7541654319073956 1101 50-51 1.7089084395842846 1101 52-53 1.94789478561718 1101 54-55 2.284707872780288 1101 56-57 2.4021109024180873 1101 58-59 2.3313363399649845 1101 60-61 2.190093854410634 1101 62-63 2.421817887987082 1101 64-65 2.4422221205188883 1101 66-67 2.7093658660841378 1101 68-69 2.3013404744231316 1101 70-71 2.3895751774953773 1101 72-73 2.256217512882227 1101 74-75 2.509643596374257 1101 76-77 2.556126877233872 1101 78-79 2.710762733139088 1101 80-81 2.7742909753861262 1101 82-83 2.9084859999527133 1101 84-85 3.053803273946791 1101 86-87 3.211542194440913 1101 88-89 2.882339186604561 1101 90-91 3.1397389288454036 1101 92-93 3.2443074327718335 1101 94-95 2.856616604014107 1101 96-97 3.159032671780757 1101 98-99 3.080898840281865 1101 100-101 3.5110401755464054 1102 1 0.00448679734162738 1102 2 0.044437474568695734 1102 3 -0.10266983405870889 1102 4 -0.0861211701972735 1102 5 -0.003048818722000135 1102 6 0.19121612389722742 1102 7 0.15745669080011737 1102 8 0.10809342901809771 1102 9 0.22680327273841527 1102 10-11 0.22141273196232447 1102 12-13 0.3001616048010476 1102 14-15 0.4423308850748455 1102 16-17 0.32901210147603877 1102 18-19 0.3595867733057432 1102 20-21 0.44333303446972394 1102 22-23 0.48251487268282034 1102 24-25 0.5485841083309779 1102 26-27 0.5784897818024888 1102 28-29 0.47446923617975045 1102 30-31 0.8364731408777928 1102 32-33 0.9239831136046135 1102 34-35 0.915895743810033 1102 36-37 0.7863298374350549 1102 38-39 1.0408763274908068 1102 40-41 1.0946708447648987 1102 42-43 1.1953965544668534 1102 44-45 1.5883422545636634 1102 46-47 1.7545580401165566 1102 48-49 1.8770454075470369 1102 50-51 2.0337882311049 1102 52-53 2.221090242126138 1102 54-55 2.314437784332341 1102 56-57 2.316415888502938 1102 58-59 2.505642175829955 1102 60-61 2.5872828525906897 1102 62-63 2.7450565416521115 1102 64-65 2.717203885653827 1102 66-67 2.8736673114184903 1102 68-69 3.014674232646531 1102 70-71 2.6987499701844335 1102 72-73 2.693787000110735 1102 74-75 2.7588504682628177 1102 76-77 2.795175335945178 1102 78-79 2.6626651783813813 1102 80-81 2.538278710226404 1102 82-83 2.568887308614098 1102 84-85 2.383746338678794 1102 86-87 2.5017244014615123 1102 88-89 2.5657199659945604 1102 90-91 2.911295017874032 1102 92-93 2.863915754951801 1102 94-95 3.0295149915563293 1102 96-97 3.2343763016165354 1102 98-99 3.310348324394514 1102 100-101 3.0962872845236262 1103 1 -0.03788493439057561 1103 2 -0.16680188678833474 1103 3 -0.2587563469430414 1103 4 -0.15504601109685012 1103 5 -0.5510030732944742 1103 6 -0.2309912876169804 1103 7 -0.15581927397661133 1103 8 -0.18028678413813282 1103 9 0.16012559634189927 1103 10-11 -0.029199192754710168 1103 12-13 0.047759615971990854 1103 14-15 -0.22427778390779451 1103 16-17 -0.3522414742286344 1103 18-19 -0.20313919648435075 1103 20-21 -0.31692518301569095 1103 22-23 -0.32587749049670833 1103 24-25 -0.20689372377457715 1103 26-27 -0.4500244354941856 1103 28-29 -0.6083779979205346 1103 30-31 -0.384512780430164 1103 32-33 -0.09700221789638164 1103 34-35 -0.21296822901165768 1103 36-37 -0.20586111846508004 1103 38-39 -0.34466278555748886 1103 40-41 -0.11847359306060667 1103 42-43 0.16543436109274978 1103 44-45 0.3924939057282373 1103 46-47 0.36960873402443895 1103 48-49 0.3058734021443996 1103 50-51 0.3951953925409626 1103 52-53 0.5504885800501285 1103 54-55 0.817159743435127 1103 56-57 0.6516267739801194 1103 58-59 0.7686547918007491 1103 60-61 1.021183187011232 1103 62-63 0.5575657573858024 1103 64-65 0.7962203235737029 1103 66-67 0.6885003665595413 1103 68-69 0.816850030507883 1103 70-71 1.0031316460619202 1103 72-73 1.0091331873036644 1103 74-75 0.8586369581446824 1103 76-77 0.6331229256990518 1103 78-79 1.0200292131328048 1103 80-81 0.8373934099851716 1103 82-83 0.5925267714179228 1103 84-85 0.5148309346905187 1103 86-87 0.7797476044671718 1103 88-89 0.46951065530194924 1103 90-91 0.6480759694685752 1103 92-93 0.7183455000962162 1103 94-95 0.7880273804514459 1103 96-97 0.9108317977326585 1103 98-99 0.882434530579232 1103 100-101 1.0269466297199408 1104 1 -0.018622558478412543 1104 2 0.0920349199085777 1104 3 0.1406366362051159 1104 4 -0.09838745259752102 1104 5 -0.02622933499105784 1104 6 -0.05357582073993683 1104 7 -0.006683779926255795 1104 8 0.2682044393185734 1104 9 0.12231304840509694 1104 10-11 0.03122308936567464 1104 12-13 0.045705181681015006 1104 14-15 0.10884188825927765 1104 16-17 0.19171246261532104 1104 18-19 0.2160694902116873 1104 20-21 0.014978264051116241 1104 22-23 0.10714795161754864 1104 24-25 0.10674443282262303 1104 26-27 0.2163409141431316 1104 28-29 0.45916084458379913 1104 30-31 0.2925849339432034 1104 32-33 0.3609394032988007 1104 34-35 0.5897896435805379 1104 36-37 0.5881969097162809 1104 38-39 0.5569763790821298 1104 40-41 0.31535718729571727 1104 42-43 0.29700033308670015 1104 44-45 0.764949146453155 1104 46-47 1.2045856147905525 1104 48-49 0.9590355874050687 1104 50-51 0.2793362555129306 1104 52-53 0.602879393715682 1104 54-55 0.7247978562043897 1104 56-57 1.077223381698225 1104 58-59 0.9818612432722702 1104 60-61 0.718529405756442 1104 62-63 0.8946847283059682 1104 64-65 1.1088887871855597 1104 66-67 1.3148398577172564 1104 68-69 1.6244125400952392 1104 70-71 1.5632471326615338 1104 72-73 1.4473138898654092 1104 74-75 1.2866386236060805 1104 76-77 1.452902471203405 1104 78-79 1.6622782434398218 1104 80-81 1.6606079696240315 1104 82-83 1.8467999947431473 1104 84-85 1.8658050104690105 1104 86-87 1.5354233216017015 1104 88-89 1.6606168722940389 1104 90-91 1.5053557888974964 1104 92-93 1.5645971162511891 1104 94-95 1.6479971391786812 1104 96-97 1.262671475159081 1104 98-99 0.6613116589731582 1104 100-101 1.6516590089192036 1105 1 -0.09893614732858325 1105 2 -0.13647843665356163 1105 3 -0.18247602349291014 1105 4 -0.308550054223538 1105 5 -0.27175779027560765 1105 6 -0.2641449264309941 1105 7 0.028682073732284152 1105 8 -0.04120322618665284 1105 9 -0.027745023604190067 1105 10-11 -0.009253101110509476 1105 12-13 -0.005340114486230618 1105 14-15 0.1687141298388326 1105 16-17 0.05611432091961177 1105 18-19 0.020715250955007036 1105 20-21 -0.017396881128895814 1105 22-23 0.28092843942242496 1105 24-25 -0.14099075881500767 1105 26-27 -0.1765743007233027 1105 28-29 0.10570556003094822 1105 30-31 0.05524463196875473 1105 32-33 0.1341972430200613 1105 34-35 -0.30751000798739625 1105 36-37 -0.34885303221170716 1105 38-39 -0.30066009013970074 1105 40-41 0.11117600262672767 1105 42-43 0.0022268139229311146 1105 44-45 0.20664484912446568 1105 46-47 -0.28005433875184593 1105 48-49 0.19387879297998012 1105 50-51 0.08906331706926096 1105 52-53 -0.05490225552938455 1105 54-55 -0.013500633923356986 1105 56-57 0.17905264999090065 1105 58-59 0.5677787810190793 1105 60-61 0.5215201142349528 1105 62-63 0.5198299083292 1105 64-65 0.6053173586141334 1105 66-67 0.8824356765325838 1105 68-69 0.94656701163996 1105 70-71 1.026110082719601 1105 72-73 0.9040792789478722 1105 74-75 0.6566153948023619 1105 76-77 0.715939637289349 1105 78-79 1.2429402643457443 1105 80-81 0.668592870901616 1105 82-83 0.5219742107710204 1105 84-85 0.8065425249870124 1105 86-87 0.8468635074321291 1105 88-89 0.5604136202615209 1105 90-91 0.37280642768959993 1105 92-93 0.07777946236036826 1105 94-95 0.10574975241910067 1105 96-97 0.21783988398602716 1105 98-99 0.4688900561856997 1105 100-101 0.9460840960272208 1106 1 0.19746290816137702 1106 2 -0.006903466169497108 1106 3 -0.11411710967944799 1106 4 -0.21392195504998313 1106 5 -0.03704704647395829 1106 6 0.052383999188840846 1106 7 -0.45024354610242767 1106 8 -0.24923156148771852 1106 9 -0.11759154072343847 1106 10-11 -0.2917796654552589 1106 12-13 -0.691998910235931 1106 14-15 -0.02266721608796729 1106 16-17 0.10747205409553118 1106 18-19 0.08818868661025903 1106 20-21 -0.23183017510765325 1106 22-23 -0.3833277589246791 1106 24-25 -0.3530922936438188 1106 26-27 -0.44298516258870535 1106 28-29 -0.1891291507128514 1106 30-31 0.14963660363345355 1106 32-33 0.24438024183941565 1106 34-35 0.3238654347516743 1106 36-37 0.4154326820740053 1106 38-39 0.66861381777057 1106 40-41 0.7447061088841096 1106 42-43 0.39461170176237204 1106 44-45 0.9069400085105315 1106 46-47 1.1986056376354028 1106 48-49 1.5526155108076054 1106 50-51 1.8455863395013736 1106 52-53 1.8916148637150059 1106 54-55 2.0614403341994247 1106 56-57 1.1838047161066 1106 58-59 1.4413620159659501 1106 60-61 1.311219051661169 1106 62-63 1.6762643829454973 1106 64-65 1.832029283053327 1106 66-67 2.2228725124239723 1106 68-69 2.1914017223837625 1106 70-71 1.8591384180206667 1106 72-73 2.5676861266456363 1106 74-75 2.582412325144748 1106 76-77 2.6441273586588885 1106 78-79 2.6273558487613293 1106 80-81 2.552447652483668 1106 82-83 2.4818030183271027 1106 84-85 2.61135362297993 1106 86-87 1.5631563055431101 1106 88-89 2.283760727699537 1106 90-91 1.6586682930969197 1106 92-93 1.589054550908184 1106 94-95 1.9583176390778938 1106 96-97 2.0397997423095227 1106 98-99 1.929604341899985 1106 100-101 1.3026366343979419 1107 1 -0.5071779055703409 1107 2 -0.43373118390630694 1107 3 -0.004454045470929202 1107 4 -0.0431654388389191 1107 5 -0.17175779027560623 1107 6 -0.37183723412330494 1107 7 -0.29824100319079605 1107 8 -0.1269175119009418 1107 9 -0.27499777085694177 1107 10-11 -0.19826409012150492 1107 12-13 -0.17896648811260718 1107 14-15 -0.28678037565567394 1107 16-17 -0.07905051424521758 1107 18-19 -0.44906496882521907 1107 20-21 -0.13195732068934518 1107 22-23 -0.6825330990391123 1107 24-25 -0.5365951544194019 1107 26-27 -0.6463545205035217 1107 28-29 -0.11160213227673665 1107 30-31 -0.12442569770157519 1107 32-33 -0.577615943793127 1107 34-35 -0.24294956842695825 1107 36-37 -0.4153365486952296 1107 38-39 -0.4355502000298088 1107 40-41 -0.9794833380326153 1107 42-43 -0.645025933329805 1107 44-45 -0.4504980080183927 1107 46-47 -0.8220873057848124 1107 48-49 -0.5890882399870563 1107 50-51 -0.4634092104032632 1107 52-53 -0.7983088489359744 1107 54-55 -0.5555336009563234 1107 56-57 -0.8882550423167928 1107 58-59 -0.6506278123875191 1107 60-61 -0.8430403253254859 1107 62-63 -0.5428074543081607 1107 64-65 -0.2218804435836681 1107 66-67 -0.4384434443465359 1107 68-69 -0.2976637575908079 1107 70-71 -0.047791016181495394 1107 72-73 0.08402433389292696 1107 74-75 -0.023054934867968058 1107 76-77 0.07610447245418683 1107 78-79 0.8308523522578319 1107 80-81 0.8677686950774408 1107 82-83 0.27334783714464805 1107 84-85 -0.00527066182617375 1107 86-87 0.3364239469925714 1107 88-89 0.1354136202615166 1107 90-91 0.1280262079093788 1107 92-93 0.15030693488784053 1107 94-95 -0.03793156626221972 1107 96-97 -0.1612809951348524 1107 98-99 -0.7550110427153989 1107 100-101 0.09828189822501798 1108 1 -0.07246961868814594 1108 2 -0.022434268261562806 1108 3 -0.34168927401050553 1108 4 -0.0566176870737749 1108 5 -0.3500186598408277 1108 6 -0.3168019312619137 1108 7 -0.1532019842387342 1108 8 -0.4148402172149517 1108 9 0.10151653609214861 1108 10-11 -0.22843874638311235 1108 12-13 -0.2622069191791283 1108 14-15 0.05366927131571231 1108 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60-61 0.43509154280638285 2316 62-63 0.30554419404348465 2316 64-65 0.47746021575698805 2316 66-67 0.11529281938972957 2316 68-69 0.4515670116399626 2316 70-71 0.545395797005316 2316 72-73 -0.1852064353378431 2316 74-75 -0.22767031948334804 2316 76-77 0.10665392300363763 2316 78-79 0.15722597863146248 2316 80-81 0.29216429947304334 2316 82-83 0.41268849648530903 2316 84-85 0.40868538212987104 2316 86-87 0.6382920788607009 2316 88-89 0.6054136202615226 2316 90-91 1.1249492848324572 2316 92-93 0.5906366052175116 2316 94-95 -0.05067881900947313 2316 96-97 0.3521255982717406 2316 98-99 0.26389005618569783 2316 100-101 0.2803698103129335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 980.0 3 153.0 4 130.0 5 134.0 6 149.0 7 148.0 8 200.0 9 257.0 10 336.0 11 518.0 12 851.0 13 1224.0 14 1490.0 15 2138.0 16 3277.0 17 5164.0 18 4235.0 19 2236.0 20 2044.0 21 1824.0 22 1835.0 23 1912.0 24 2071.0 25 2001.0 26 2229.0 27 2378.0 28 2593.0 29 3080.0 30 3535.0 31 4503.0 32 5655.0 33 7956.0 34 11670.0 35 19156.0 36 27903.0 37 44252.0 38 45484.0 39 8856.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03333958506372 20.723146585215552 16.5117130634372 19.731800766283524 2 14.702902011900882 22.097430105750902 42.1829593289795 21.01670855336872 3 17.1321944011678 28.958845770966608 32.41314042613978 21.495819401725807 4 10.92124117796735 18.640882448786456 39.91241579729748 30.525460575948713 5 10.92274936729201 39.019447680517054 34.863884090574324 15.19391886161661 6 26.198804318729362 39.90318550905684 18.629874185776746 15.268135986437049 7 23.91624428897773 37.1319067675614 22.308234580239862 16.643614363221015 8 25.413708946999748 34.01269793112064 20.764195938982112 19.809397182897502 9 26.980338269214464 16.287069300128042 21.777363356101738 34.955229074555746 10-11 22.90578573611796 28.781432456612 27.667701121986155 20.645080685283887 12-13 25.32188649694233 27.3055238868673 28.83937914001477 18.5332104761756 14-15 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35.77659951774119 18.503168587597337 40-41 17.55993540270702 28.245924749507047 35.741307470623404 18.452832377162537 42-43 17.654852452434593 28.18838801775478 35.26041085807329 18.896348671737336 44-45 17.675508064336228 28.626943583108392 34.765654627791285 18.931893724764095 46-47 17.981575660242687 28.852159172019988 34.12183261955746 19.04443254817987 48-49 18.004987753681704 29.166982676701657 33.54442803670772 19.283601532908914 50-51 18.149757527359036 29.459199014940957 32.85203146121553 19.539011996484483 52-53 18.35075234515298 29.58227833982131 32.58875888146521 19.478210433560506 54-55 18.099903848513442 29.424139600357833 32.671204302946336 19.804752248182382 56-57 18.138445581986684 29.449407251274156 32.56292727536329 19.849219891375867 58-59 18.166651797662592 28.884155589258633 32.83321438130074 20.115978231778037 60-61 18.14947571750084 28.846355450374396 33.07095044465312 19.933218387471644 62-63 18.173971035310334 28.398823211899927 33.587976954938114 19.839228797851625 64-65 18.059080953039476 28.14888007601476 33.52522895876735 20.26681001217842 66-67 18.12084129848519 28.30493389574555 33.24217556272822 20.33204924304104 68-69 18.034037427787567 27.95307028305043 33.27504293712221 20.73784935203979 70-71 18.019999420048048 27.967736826725968 33.324485348406135 20.68777840481985 72-73 18.240852692772723 27.608560835560393 33.21651489936958 20.9340715722973 74-75 17.78166383019537 27.920914867942372 32.889832644476016 21.40758865738624 76-77 17.5917003488673 27.39076706193064 33.37192287445239 21.645609714749682 78-79 17.895572726137154 27.08582415817556 33.44679598137012 21.571807134317172 80-81 17.665899398676014 27.291990079046446 33.14575415306104 21.896356369216495 82-83 17.778794030383473 27.108885270038147 32.95045396747496 22.16186673210342 84-85 17.56960816896445 27.034936241303857 32.78559814065947 22.609857449072226 86-87 17.548539629080285 27.06241845007751 32.670376607300184 22.718665313542026 88-89 17.97454067714572 26.608048215237673 32.48059992460793 22.93681118300868 90-91 18.142460436950046 26.833729242642217 32.408578406771724 22.61523191363601 92-93 18.30785154385292 26.781085626843097 31.992174778158976 22.918888051145007 94-95 18.362416825969703 26.627511950516052 31.7133125291374 23.296758694376845 96-97 18.96800526368614 27.0136387460829 30.75710095794627 23.261255032284687 98-99 19.395008665356638 27.276802940645094 30.163693828526313 23.164494565471955 100-101 19.986394861272192 26.780934962976175 29.842537246855205 23.390132928896424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 605.0 1 399.0 2 250.5 3 310.5 4 245.5 5 238.0 6 476.0 7 1129.0 8 1873.0 9 2011.0 10 1539.0 11 1098.0 12 1011.0 13 1023.0 14 1012.0 15 1023.5 16 1054.5 17 1054.0 18 1084.5 19 1131.0 20 1194.5 21 1351.0 22 1506.0 23 1640.5 24 1820.0 25 2054.0 26 2388.5 27 2806.5 28 3157.5 29 3435.0 30 3840.5 31 4348.5 32 4878.0 33 5549.5 34 6327.5 35 6848.5 36 7353.0 37 7800.0 38 7844.0 39 7819.0 40 7641.0 41 7557.0 42 7634.5 43 7843.5 44 7992.5 45 7878.0 46 7493.5 47 7031.0 48 6846.5 49 6507.0 50 6042.0 51 5498.5 52 5036.0 53 4796.0 54 4428.5 55 3954.0 56 3443.5 57 2915.0 58 2555.0 59 2155.5 60 1680.5 61 1362.0 62 1096.5 63 804.5 64 560.0 65 414.5 66 295.5 67 221.5 68 171.5 69 119.0 70 88.0 71 65.0 72 52.5 73 42.0 74 32.5 75 23.0 76 14.0 77 12.0 78 10.5 79 6.5 80 4.0 81 1.5 82 2.5 83 2.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27698610616316355 2 0.24180619878874243 3 0.24447809048806554 4 0.2435874599216245 5 0.23289989312433204 6 0.18703241895261846 7 0.1923762023512647 8 0.19104025650160314 9 0.18658710366939793 10-11 0.1997239045244033 12-13 0.20105985037406482 14-15 0.18591913074456717 16-17 0.19504809405058782 18-19 0.18079800498753118 20-21 0.18436052725329533 22-23 0.1856964731029569 24-25 0.17879408621303883 26-27 0.1879230495190595 28-29 0.1834698966868543 30-31 0.18480584253651586 32-33 0.1868097613110082 34-35 0.18035268970431065 36-37 0.18391521197007482 38-39 0.18102066262914143 40-41 0.17901674385464908 42-43 0.17589953687210544 44-45 0.18925899536872104 46-47 0.17812611328820804 48-49 0.18302458140363378 50-51 0.18302458140363378 52-53 0.19037228357677236 54-55 0.193489490559316 56-57 0.17478624866405415 58-59 0.16921980762379765 60-61 0.17656750979693625 62-63 0.1738956180976131 64-65 0.1750089063056644 66-67 0.18102066262914143 68-69 0.17589953687210544 70-71 0.17968471677947986 72-73 0.18836836480228 74-75 0.17656750979693625 76-77 0.18079800498753118 78-79 0.17990737442109012 80-81 0.17278232988956183 82-83 0.18925899536872104 84-85 0.1756768792304952 86-87 0.17255967224795155 88-89 0.1774581403633773 90-91 0.17478624866405415 92-93 0.18391521197007482 94-95 0.18102066262914143 96-97 0.17033309583184894 98-99 0.17434093338083362 100-101 0.16921980762379765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 224560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.61196050903568 #Duplication Level Percentage of deduplicated Percentage of total 1 78.54714790582406 43.681608874360734 2 12.179701502162587 13.546741579002166 3 3.5270208374740215 5.884336305844538 4 1.6324515744214012 3.6313532995854434 5 0.9678652009480794 2.691244066659724 6 0.6238213223840522 2.0815156047069796 7 0.4292110236372891 1.6708486547591792 8 0.35229392014638833 1.5673404459803457 9 0.23319255357998728 1.1671465572622297 >10 1.4305524103564942 13.671645294728762 >50 0.04820956031045756 1.8296649348764613 >100 0.022628977288582118 2.192424459943176 >500 0.00295160573329332 1.050525321460169 >1k 0.00295160573329332 5.333604600830067 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4318 1.922871392946206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3134 1.39561809761311 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2296 1.022443890274314 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 678 0.3019237620235126 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 641 0.2854470965443534 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 601 0.2676344852155326 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 419 0.18658710366939793 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 374 0.16654791592447454 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 338 0.1505165657285358 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 307 0.1367117919486997 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 278 0.12379764873530459 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.2265764161026006E-4 0.0 20-21 0.0 0.0 0.0 8.906305664410402E-4 0.0 22-23 0.0 0.0 0.0 0.0020039187744923404 0.0 24-25 0.0 0.0 0.0 0.004453152832205202 0.0 26-27 0.0 0.0 0.0 0.005566441040256501 0.0 28-29 0.0 0.0 0.0 0.011800855005343783 0.0 30-31 0.0 0.0 0.0 0.027832205201282507 0.0 32-33 0.0 0.0 0.0 0.05766832917705736 0.0 34-35 0.0 0.0 0.0 0.09596544353402209 0.0 36-37 0.0 0.0 0.0 0.15808692554328463 0.0 38-39 0.0 0.0 0.0 0.26785714285714285 0.0 40-41 0.0 0.0 0.0 0.41013537584609905 0.0 42-43 0.0 0.0 0.0 0.5646597791236195 0.0 44-45 0.0 0.0 0.0 0.7574812967581047 0.0 46-47 0.0 0.0 0.0 1.0255610972568578 0.0 48-49 0.0 0.0 0.0 1.3241449946562165 0.0 50-51 0.0 0.0 0.0 1.6708229426433916 0.0 52-53 0.0 0.0 0.0 2.0126024225151404 0.0 54-55 0.0 0.0 0.0 2.4064837905236907 0.0 56-57 0.0 0.0 0.0 2.9030103313145705 0.0 58-59 0.0 0.0 0.0 3.398200926255789 0.0 60-61 0.0 0.0 0.0 3.9884663341645883 0.0 62-63 0.0 0.0 0.0 4.677146419665123 0.0 64-65 0.0 0.0 0.0 5.384084431777699 0.0 66-67 0.0 0.0 0.0 6.162272889205558 0.0 68-69 0.0 0.0 0.0 7.095431065194157 0.0 70-71 0.0 0.0 0.0 8.313591022443891 0.0 72-73 0.0 0.0 0.0 9.468293551834698 0.0 74-75 0.0 0.0 0.0 10.613421802636267 0.0 76-77 0.0 0.0 0.0 12.024625935162096 0.0 78-79 0.0 0.0 0.0 13.487931955824724 0.0 80-81 0.0 0.0 0.0 15.020929818311364 0.0 82-83 0.0 0.0 0.0 16.49848592803705 0.0 84-85 0.0 0.0 0.0 18.026140007125043 0.0 86-87 0.0 0.0 0.0 19.463840399002493 0.0 88-89 0.0 0.0 0.0 21.24933202707517 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 900 0.0 66.59302 1 GTATCAA 2070 0.0 51.93244 1 GTGGTAT 280 0.0 49.265244 1 TGGTATC 290 0.0 47.555805 2 TCCGGTG 60 1.474873E-6 47.555805 2 TATCAAC 2700 0.0 39.80597 2 ATCAACG 2735 0.0 39.47045 3 TCAACGC 2780 0.0 38.831535 4 CAACGCA 2835 0.0 38.07819 5 AACCCGA 50 0.0016125893 38.002144 6 AACGCAG 2900 0.0 37.183132 6 ACTATAC 55 0.0025604167 34.586037 3 TAAGGAC 55 0.0025604167 34.586037 4 TAGACCC 55 0.0025744953 34.547405 9 TATGAGG 70 2.0339867E-4 33.968433 2 CGCAGAG 3290 0.0 32.775406 8 ACGCAGA 3315 0.0 32.671528 7 TTCCGGT 90 2.3814771E-5 31.710962 1 CTTACAC 120 1.4812758E-7 31.703869 3 GCAGAGT 3385 0.0 31.434566 9 >>END_MODULE