FastQCFastQC Report
Thu 26 May 2016
SRR937169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224560
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31141.3867117919486998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25851.1511400071250444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20150.8973102956893482No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12910.5749020306376915No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.2524937655860349No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.23512646954043462No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.1861417883861774No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.12424296401852511No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.11711791948699679No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.11311008193801211No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGT200.002072128771.2488
GGTATCA11050.064.477821
ATCACGC301.2991368E-463.3315543
TATCACG250.005016588656.9983942
CACGCAG451.512282E-552.776295
TCACGCA405.3796126E-447.4986654
GTATCAA20550.042.0669441
GGTACTC500.00161327137.9989283
GTATCAC500.00161327137.9989281
GTATAGT500.00161327137.9989281
TAAGGTG500.00161327137.9989285
GTGGTAC500.00161327137.9989281
TGGTATC3900.037.755352
GTGGTAT4300.034.243221
TATCAAC27700.031.0370352
ATCAACG28450.030.2188343
TCAACGC28700.029.9556054
CAACGCA29250.029.3923365
AACGCAG30150.028.5149546
GCGCTTG350.00838737327.14209668-69