##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937169_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 224560 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.405780192376202 31.0 31.0 34.0 30.0 34.0 2 31.638216957605984 31.0 31.0 34.0 30.0 34.0 3 31.62992518703242 31.0 31.0 34.0 30.0 34.0 4 35.494050587816176 37.0 35.0 37.0 33.0 37.0 5 35.34808069112932 37.0 35.0 37.0 33.0 37.0 6 35.449902030637695 37.0 35.0 37.0 33.0 37.0 7 35.38005432846455 37.0 35.0 37.0 33.0 37.0 8 35.36389383683648 37.0 35.0 37.0 33.0 37.0 9 36.867358389739934 39.0 37.0 39.0 33.0 39.0 10-11 36.78495057000356 39.0 37.0 39.0 32.0 39.0 12-13 36.67809271464196 38.0 35.0 39.0 32.0 39.0 14-15 37.52767634485215 39.0 36.0 41.0 32.0 41.0 16-17 37.517792572141076 39.0 36.0 40.0 32.0 41.0 18-19 37.65341779479872 39.0 37.0 40.5 32.0 41.0 20-21 37.6015073922337 39.0 36.0 41.0 32.0 41.0 22-23 37.435602956893476 39.0 36.0 40.0 32.0 41.0 24-25 37.253063769148554 39.0 36.0 40.0 32.0 41.0 26-27 37.05596277164233 39.0 36.0 40.0 31.0 41.0 28-29 36.87451237976487 39.0 35.5 40.0 31.0 41.0 30-31 36.730354916280724 38.5 35.0 40.0 31.0 41.0 32-33 36.45627894549341 38.0 35.0 40.0 30.5 41.0 34-35 36.2061676166726 38.0 35.0 40.0 30.0 41.0 36-37 35.89223592803705 38.0 35.0 40.0 30.0 41.0 38-39 35.62948209832561 38.0 35.0 40.0 29.5 41.0 40-41 35.31481786604917 38.0 34.5 40.0 28.5 41.0 42-43 34.89256991449946 38.0 34.0 40.0 26.5 41.0 44-45 34.243921446384036 38.0 33.0 40.0 23.5 41.0 46-47 33.47324991093694 38.0 33.0 40.0 19.5 41.0 48-49 32.86953598147488 37.0 33.0 40.0 11.5 41.0 50-51 32.47534957249732 37.0 32.0 40.0 7.0 41.0 52-53 32.14391031350196 37.0 31.5 40.0 2.0 41.0 54-55 31.832545867474174 36.5 31.0 40.0 2.0 41.0 56-57 31.496306109725687 36.0 31.0 39.0 2.0 40.0 58-59 31.16567064481653 36.0 30.5 39.0 2.0 40.0 60-61 30.727426968293553 35.0 29.5 39.0 2.0 40.0 62-63 30.25291013537585 35.0 28.5 39.0 2.0 40.0 64-65 29.81162050231564 35.0 28.0 38.0 2.0 40.0 66-67 29.288439615247597 34.5 27.5 38.0 2.0 40.0 68-69 28.782240826505166 34.0 26.0 37.5 2.0 39.5 70-71 28.30780860349127 34.0 26.0 37.0 2.0 39.0 72-73 27.78582116138226 33.5 25.5 36.0 2.0 39.0 74-75 27.26927101888137 33.0 24.5 36.0 2.0 39.0 76-77 26.163274848592803 31.5 23.0 34.5 2.0 37.0 78-79 26.764189971499825 33.0 25.0 35.0 2.0 37.0 80-81 26.581775472034202 33.0 24.0 35.0 2.0 37.0 82-83 26.19548450302814 33.0 22.5 35.0 2.0 36.5 84-85 25.732292037762736 32.0 21.0 35.0 2.0 36.0 86-87 25.207361061631637 32.0 19.0 34.5 2.0 35.5 88-89 24.679508817242606 31.5 16.5 34.0 2.0 35.0 90-91 24.049165033843963 31.0 8.5 34.0 2.0 35.0 92-93 23.450438635553972 31.0 2.0 34.0 2.0 35.0 94-95 22.800115781973638 30.0 2.0 34.0 2.0 35.0 96-97 22.055931599572496 30.0 2.0 34.0 2.0 35.0 98-99 21.229978624866405 29.0 2.0 34.0 2.0 35.0 100-101 19.367017723548273 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26638020971853393 1101 2 0.27791675384106185 1101 3 0.11476041635119572 1101 4 0.1529444694676485 1101 5 0.13874854309879936 1101 6 0.12526300972001536 1101 7 0.19976880920660278 1101 8 0.08589419018628064 1101 9 0.34826171482869483 1101 10-11 0.17160551873653418 1101 12-13 0.12670153924660355 1101 14-15 0.3586740538561486 1101 16-17 0.08818800220029033 1101 18-19 0.08316525524518426 1101 20-21 0.18485797635662493 1101 22-23 0.2717409862900837 1101 24-25 0.39221741121376397 1101 26-27 0.25583158687194185 1101 28-29 0.15243511091676254 1101 30-31 0.2461464921182568 1101 32-33 0.40546240257017274 1101 34-35 0.4683377017141126 1101 36-37 0.47554391263119555 1101 38-39 0.1807582951012776 1101 40-41 0.19626235693399252 1101 42-43 0.4144070319790174 1101 44-45 0.1491824922434546 1101 46-47 0.5643537061066937 1101 48-49 0.5194044531090398 1101 50-51 0.6296791644574498 1101 52-53 0.7894702105505651 1101 54-55 0.9912005484559181 1101 56-57 0.9701941281630049 1101 58-59 0.9201195283907921 1101 60-61 0.7344891170857331 1101 62-63 0.9274752510741777 1101 64-65 1.2322247011039273 1101 66-67 1.0918654668575591 1101 68-69 1.0775092278699425 1101 70-71 0.8558329927701678 1101 72-73 0.7110627624478418 1101 74-75 0.5448491964795323 1101 76-77 0.9629468269994916 1101 78-79 1.224112181699617 1101 80-81 0.8657555775871053 1101 82-83 1.0327786753174841 1101 84-85 1.121031766431095 1101 86-87 0.9737334300690961 1101 88-89 0.9294543124293178 1101 90-91 0.5259850446011143 1101 92-93 0.6843704167350566 1101 94-95 0.7316694170094067 1101 96-97 0.6501830471785723 1101 98-99 0.9889949983841042 1101 100-101 1.1491589579550379 1102 1 0.051821351823015505 1102 2 0.04011995043061134 1102 3 0.10605725162839974 1102 4 0.20497579802805177 1102 5 0.05786717948496545 1102 6 0.12014308450066125 1102 7 0.17105686342257798 1102 8 0.19387645810778764 1102 9 0.28614986960197797 1102 10-11 0.1587047942989841 1102 12-13 -0.05204439774220049 1102 14-15 0.2551832279406909 1102 16-17 0.11244391348324712 1102 18-19 0.3750240076263438 1102 20-21 0.2330847685074886 1102 22-23 -0.06545707621124564 1102 24-25 0.3583572142064071 1102 26-27 0.2571505164322545 1102 28-29 0.09003469452450474 1102 30-31 0.5603880987692094 1102 32-33 0.24884615619058792 1102 34-35 0.22109240560114785 1102 36-37 0.3584530640549275 1102 38-39 0.17773626455168312 1102 40-41 0.22503626585172753 1102 42-43 0.4725408440218217 1102 44-45 0.4277811340115676 1102 46-47 0.7988965598099682 1102 48-49 0.790112532393529 1102 50-51 0.8181055133063495 1102 52-53 0.9564254483827774 1102 54-55 0.9377900975767481 1102 56-57 1.0897617317122545 1102 58-59 1.024382237914704 1102 60-61 1.009996683669545 1102 62-63 1.2282053538267554 1102 64-65 1.527995982784791 1102 66-67 1.2339823399499785 1102 68-69 1.572722133197356 1102 70-71 1.8203617542864556 1102 72-73 1.47934188689106 1102 74-75 1.214776894354376 1102 76-77 1.263868130278933 1102 78-79 1.3675904733583124 1102 80-81 1.645214621726101 1102 82-83 1.8548311775658597 1102 84-85 1.277465664160058 1102 86-87 1.0659611286520878 1102 88-89 1.164657516100366 1102 90-91 1.1823215716913964 1102 92-93 1.4515058787675912 1102 94-95 1.320301483462213 1102 96-97 1.1228537644638052 1102 98-99 1.6631999228598957 1102 100-101 1.7535509048721067 1103 1 -0.02615112778440931 1103 2 -0.2222969605144378 1103 3 -0.07698933722306833 1103 4 -0.1325648249260567 1103 5 -0.22805943673354534 1103 6 -0.2599462122725029 1103 7 -0.2830119198646557 1103 8 -0.21135630920746706 1103 9 -0.23565824260289503 1103 10-11 -0.3817205744778178 1103 12-13 -0.34190207732367384 1103 14-15 -0.309713231448157 1103 16-17 -0.17602182168162983 1103 18-19 -0.2573493165846443 1103 20-21 -0.26323778512070817 1103 22-23 -0.22295322772085768 1103 24-25 -0.1482574708071951 1103 26-27 -0.08741765640477439 1103 28-29 -0.4133213070561723 1103 30-31 -0.562156092516986 1103 32-33 -0.8076042675857096 1103 34-35 -0.8494838947757373 1103 36-37 -0.8105738134480589 1103 38-39 -0.6042297979560587 1103 40-41 -0.2297815722698573 1103 42-43 -0.25189544797980545 1103 44-45 -0.7877608690908176 1103 46-47 -0.6093088345644517 1103 48-49 -0.17165618070667676 1103 50-51 0.14709688443058155 1103 52-53 -0.29518030105543147 1103 54-55 -0.21574255118731145 1103 56-57 -0.6472964291516945 1103 58-59 -0.3713192489886339 1103 60-61 0.05716944402508162 1103 62-63 -0.27473778288459627 1103 64-65 -0.31463762323595645 1103 66-67 -0.18104122283791924 1103 68-69 0.1647526616054087 1103 70-71 -0.2155781325885684 1103 72-73 -0.14355913760362427 1103 74-75 -0.44850821243017336 1103 76-77 -0.24648887987164514 1103 78-79 -0.0733433090147173 1103 80-81 -0.002611644736887797 1103 82-83 -0.17540527604286993 1103 84-85 9.124880620348108E-5 1103 86-87 -0.07242232844240704 1103 88-89 0.10273958184025034 1103 90-91 -0.06379897305431115 1103 92-93 -0.07284135238365508 1103 94-95 0.09470032272212237 1103 96-97 -0.20790407849419523 1103 98-99 -0.09795185216413671 1103 100-101 0.07064292065534516 1104 1 -0.20038906962474456 1104 2 -0.19575914796650196 1104 3 -0.3046096996800607 1104 4 -0.12126817557142289 1104 5 -0.013631281670328121 1104 6 -0.24272183517697954 1104 7 0.027695243810754278 1104 8 -0.12272527464914873 1104 9 0.32308214264462265 1104 10-11 -0.06740308494173775 1104 12-13 -0.4561779303247846 1104 14-15 -0.0891710799349994 1104 16-17 -0.3299783882014822 1104 18-19 -0.26445491998569537 1104 20-21 -0.5307470437712496 1104 22-23 -0.30879023164345654 1104 24-25 -0.1622659734259031 1104 26-27 -0.044685423152529324 1104 28-29 -0.311492586612637 1104 30-31 0.007453619752645579 1104 32-33 -0.03239772845832789 1104 34-35 -0.011455494276098932 1104 36-37 0.0015522795109816911 1104 38-39 -0.25467465146119395 1104 40-41 -0.31238078774988765 1104 42-43 -0.25758760058866415 1104 44-45 -0.2872568478780977 1104 46-47 0.05448710258629319 1104 48-49 0.28282303389678987 1104 50-51 0.27085052762738826 1104 52-53 -0.19657841207158455 1104 54-55 -0.4370297775366616 1104 56-57 -0.3833262759730758 1104 58-59 -0.2801133234522908 1104 60-61 0.1418614432361771 1104 62-63 -0.1634751001769068 1104 64-65 -0.305110526617284 1104 66-67 -0.11345855240205083 1104 68-69 0.36733083967829216 1104 70-71 0.30486211898375615 1104 72-73 0.15573084990541375 1104 74-75 -0.476640265987907 1104 76-77 -0.385817874239752 1104 78-79 -0.007042605576415895 1104 80-81 0.22545247552695713 1104 82-83 0.05073589372462095 1104 84-85 -0.339482086280249 1104 86-87 -0.20820673884715646 1104 88-89 -0.0796229628094558 1104 90-91 -0.5477579501103875 1104 92-93 -0.3580954448167617 1104 94-95 -0.1203907298056528 1104 96-97 0.2969389079410618 1104 98-99 -0.23643715885008731 1104 100-101 -0.04077315981273699 1105 1 0.399320570328797 1105 2 0.22433376724836407 1105 3 0.44039610752086844 1105 4 0.46891765485830916 1105 5 0.3528030969591782 1105 6 0.40501335646064973 1105 7 0.11294495345070743 1105 8 0.6076463862103196 1105 9 0.5410263935157005 1105 10-11 0.22670260611517534 1105 12-13 0.20984994423967152 1105 14-15 0.15383124294537254 1105 16-17 0.13253612980084029 1105 18-19 0.10006308233718642 1105 20-21 0.18507757876069064 1105 22-23 -0.10031171828689622 1105 24-25 -0.5101173090821192 1105 26-27 0.03562816569764493 1105 28-29 -0.004291657460491649 1105 30-31 0.141280565164962 1105 32-33 -0.018925022302688888 1105 34-35 0.10927621304097812 1105 36-37 -0.06926653582002729 1105 38-39 -0.22929718340080285 1105 40-41 -0.31879774477659595 1105 42-43 -0.9516932916455758 1105 44-45 -0.8307527828374504 1105 46-47 -1.5975163817342626 1105 48-49 -0.7087855607605817 1105 50-51 -1.0104502853807382 1105 52-53 -0.3229431042899371 1105 54-55 -0.16324928973178388 1105 56-57 -0.6497896906072214 1105 58-59 -0.5380982247298753 1105 60-61 -0.3970758389937892 1105 62-63 -0.7062741710247593 1105 64-65 -0.45095837795294 1105 66-67 0.16067036745857521 1105 68-69 0.5105073785865386 1105 70-71 0.5096240237456655 1105 72-73 0.3517238812643022 1105 74-75 0.7204270975428706 1105 76-77 0.7061391524733729 1105 78-79 0.6620340494758494 1105 80-81 0.36241530944101186 1105 82-83 0.13726318756898692 1105 84-85 0.6085144293991931 1105 86-87 0.35837281980557023 1105 88-89 0.9700576411394408 1105 90-91 0.9409180080777659 1105 92-93 0.3886815586675567 1105 94-95 0.09685665695392842 1105 96-97 0.08427920991550764 1105 98-99 -0.3009841971776126 1105 100-101 0.05905262415938495 1106 1 -0.5610690400608149 1106 2 -0.3623250285013384 1106 3 -0.2173961002713405 1106 4 -0.07836687878985771 1106 5 -0.5379879301954915 1106 6 -0.07420742276012504 1106 7 -0.6807976675646401 1106 8 -0.18981524306948927 1106 9 -0.32739155217497284 1106 10-11 -0.3020164021492917 1106 12-13 -0.2321807523364825 1106 14-15 -0.14023605339464496 1106 16-17 -0.18632455496893385 1106 18-19 -0.48458862958725746 1106 20-21 -0.5741018781460383 1106 22-23 -0.2827202188772162 1106 24-25 -0.4696804731907349 1106 26-27 -0.9632502286352889 1106 28-29 -0.565389650376666 1106 30-31 -1.1303546178338593 1106 32-33 -0.3456074308032768 1106 34-35 -0.3551865968763863 1106 36-37 0.21476534139367232 1106 38-39 0.4617299712627201 1106 40-41 -0.7061354071142603 1106 42-43 -0.13867676271996032 1106 44-45 -0.16174451837463977 1106 46-47 0.2715804305443683 1106 48-49 0.3250692208214687 1106 50-51 0.5221943427184357 1106 52-53 0.7710356442342707 1106 54-55 -0.08659639716980294 1106 56-57 0.43430121848368586 1106 58-59 0.6358982333574907 1106 60-61 1.0255038540404477 1106 62-63 1.421470811265678 1106 64-65 1.3067452820234493 1106 66-67 2.349956081744292 1106 68-69 1.0008556666621011 1106 70-71 1.1154681795898185 1106 72-73 1.4576861008628832 1106 74-75 1.5569383135995416 1106 76-77 0.9142560355902525 1106 78-79 0.8243421958749053 1106 80-81 1.5115003153442004 1106 82-83 0.5994827861522225 1106 84-85 0.6317434730827856 1106 86-87 1.1440575895812515 1106 88-89 1.4314803093802908 1106 90-91 1.6791116326350277 1106 92-93 1.7590180403676747 1106 94-95 0.8606110843447219 1106 96-97 1.5431930233747764 1106 98-99 1.8017607851128226 1106 100-101 2.0143064612314063 1107 1 -0.26386624285801474 1107 2 -0.0954464525318528 1107 3 -0.17334015621539578 1107 4 0.21781875375940984 1107 5 -0.22082327666719692 1107 6 -0.15322840178110653 1107 7 -0.28156054105478034 1107 8 -0.1599360313720979 1107 9 -0.3463362438469346 1107 10-11 -0.24445124003867136 1107 12-13 -0.186853352463622 1107 14-15 -0.3343555702414349 1107 16-17 -0.44795837569365915 1107 18-19 0.08632681860092362 1107 20-21 -0.3404718717887576 1107 22-23 -0.3629490809242597 1107 24-25 -0.5502271991920082 1107 26-27 -0.45613007606058886 1107 28-29 -0.760335613504445 1107 30-31 -0.6474556985713065 1107 32-33 -0.9985953519730231 1107 34-35 -0.6561084023436408 1107 36-37 -0.949761041314531 1107 38-39 -1.0067697108733284 1107 40-41 -0.6229186238974762 1107 42-43 -0.42669329164557723 1107 44-45 -0.6576758597605306 1107 46-47 -2.0065823158001983 1107 48-49 -1.1096097365847584 1107 50-51 -1.2104502853807375 1107 52-53 -1.1949211262679533 1107 54-55 -1.9036339051164006 1107 56-57 -1.8209435367610673 1107 58-59 -1.8641971258287775 1107 60-61 -1.3742736411915892 1107 62-63 -1.543087357837944 1107 64-65 -1.2435407955353561 1107 66-67 -1.269274687486476 1107 68-69 -1.0359211928420322 1107 70-71 -1.3810353169136746 1107 72-73 -1.0394849099444876 1107 74-75 -0.7446827925670156 1107 76-77 -0.4249048035705876 1107 78-79 -0.6058230933812894 1107 80-81 -0.9669802949545918 1107 82-83 -1.0292203289145299 1107 84-85 -0.5890130431282756 1107 86-87 -1.0836601472273948 1107 88-89 -0.9456016995199015 1107 90-91 -1.3349061677464107 1107 92-93 -1.4126920677060681 1107 94-95 -1.7921543320570628 1107 96-97 -2.0668196911833903 1107 98-99 -2.3858743070677235 1107 100-101 -1.8557825406757829 1108 1 0.04103899682983325 1108 2 0.08195971617866249 1108 3 -0.035138744100940755 1108 4 0.38827583291214296 1108 5 0.09641938405372485 1108 6 0.07423350828949538 1108 7 0.03765164772262608 1108 8 -0.08189812724723566 1108 9 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6 28.0 7 69.0 8 178.0 9 314.0 10 560.0 11 900.0 12 1306.0 13 1766.0 14 2248.0 15 3241.0 16 4360.0 17 5669.0 18 4622.0 19 2805.0 20 2460.0 21 2494.0 22 2613.0 23 2750.0 24 3174.0 25 3504.0 26 4088.0 27 4697.0 28 5581.0 29 6669.0 30 7962.0 31 9432.0 32 11331.0 33 14440.0 34 17696.0 35 23535.0 36 28651.0 37 30988.0 38 13887.0 39 525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.97255013765023 23.436622980907153 17.491825625217167 18.099001256225446 2 15.997506234413967 24.608567866049164 39.09467402921268 20.299251870324188 3 18.310028500178127 29.653099394371214 32.68346989668686 19.353402208763804 4 12.463929462059138 21.091913074456713 38.19246526540791 28.25169219807624 5 11.2457633535089 40.629495521736615 34.02812108903527 14.096620035719218 6 26.601353758460988 41.079889561809765 17.358389739935877 14.960366939793374 7 23.794531528322054 37.98004987531172 21.669932312076952 16.555486284289277 8 26.111952262201637 35.46980762379765 19.78001425008906 18.63822586391165 9 26.785268970431066 16.964285714285715 21.27627360171001 34.97417171357321 10-11 23.614178838617743 29.733256145350907 26.944469184182402 19.708095831848947 12-13 25.388268772168892 28.302706024595363 28.294690153169945 18.014335050065796 14-15 22.232365514784465 28.394415746348418 28.824367652297827 20.548851086569293 16-17 18.4244745279658 31.971633416458857 29.42732454577841 20.176567509796936 18-19 18.438501959387246 28.557623797648734 35.18124332027075 17.822630922693268 20-21 19.82988956180976 27.36395618097613 35.58892946205914 17.21722479515497 22-23 19.321554612961044 27.690680560658183 35.532547343108114 17.45521748327266 24-25 18.82525828286427 28.190684004275024 35.65661738510866 17.327440327752047 26-27 18.54726660610271 28.6814870232815 35.454630470795706 17.31661589982009 28-29 18.078440628451318 28.61809291389077 35.773228472692296 17.53023798496562 30-31 18.388403990024937 28.50507659422871 35.56310117563235 17.543418240114 32-33 18.191129319558247 29.071517634485218 35.04920733879587 17.68814570716067 34-35 18.523557178482367 28.86734057712861 35.11466868542929 17.49443355895974 36-37 18.742916688004737 28.656547596516734 34.80191218808378 17.798623527394746 38-39 18.194469184182402 29.343159957249732 34.8848859992875 17.57748485928037 40-41 18.284422871392948 29.272354827217672 34.74951015318845 17.693712148200927 42-43 18.591921900121587 29.38988923677143 33.9765201551665 18.04166870794048 44-45 18.51799073744211 29.75863911649448 33.51175632347702 18.211613822586393 46-47 18.87691485571785 30.132035981474885 32.64361417883862 18.34743498396865 48-49 18.99692732454578 30.370502315639474 31.83336302101888 18.799207338795867 50-51 19.028099394371214 30.79533309583185 30.914677591734947 19.26188991806199 52-53 19.146998574991095 31.110393658710368 30.71940684004275 19.02320092625579 54-55 18.87736017100107 31.01064303526897 30.74122728892056 19.370769504809402 56-57 19.053705023156393 31.01197898111863 30.6185429283933 19.31577306733167 58-59 19.00627894549341 30.429061275382974 30.899091556822228 19.66556822230139 60-61 18.952173138582115 30.613199144994656 30.889517278232987 19.54511043819024 62-63 18.891610260064127 30.180575347345922 31.42077841111507 19.507035981474885 64-65 18.810785536159603 30.06234413965087 31.296980762379768 19.82988956180976 66-67 18.772265764161027 30.328197363733523 30.911560384752406 19.987976487353045 68-69 18.51197898111863 29.85727645172782 31.07721767011044 20.553526897043106 70-71 18.70034734592091 29.91227288920556 30.83786070537941 20.549519059494123 72-73 18.776050944068402 29.749510153188456 30.67353936587104 20.800899536872105 74-75 18.27952440327752 29.84614356964731 30.26006412540078 21.614267901674385 76-77 18.04862842892768 29.380343783398644 31.068979337370862 21.502048450302812 78-79 17.993631991449945 29.34071072319202 30.84721232632704 21.818444959030995 80-81 17.571250445315282 29.529301745635912 30.53526897043107 22.36417883861774 82-83 17.539633060206626 29.2122372639829 30.512112575703597 22.736017100106874 84-85 17.135363089433067 29.020880926616165 30.463708869383993 23.380047114566775 86-87 17.031083006768792 29.020306376914856 30.382748485928033 23.565862130388314 88-89 16.836480228001424 28.50129141432134 30.2885643035269 24.37366405415034 90-91 16.81578701250117 28.859797184453477 30.017947884331896 24.306467918713455 92-93 16.830173051060303 28.91457886158587 29.731739684179587 24.523508403174237 94-95 16.46330602066263 28.508193801211256 29.40817598859993 25.620324189526183 96-97 16.658576772354827 28.919442465265412 28.648468115425725 25.773512646954046 98-99 17.076505165657284 29.17728001425009 28.198254364089774 25.547960456002848 100-101 16.97289689865342 28.847400030730654 27.75935670496122 26.420346365654705 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 723.0 1 477.5 2 279.0 3 342.5 4 302.5 5 319.0 6 516.5 7 847.0 8 1180.5 9 1296.5 10 1150.0 11 1006.5 12 996.5 13 1034.0 14 1089.5 15 1115.5 16 1089.0 17 1094.0 18 1144.5 19 1228.0 20 1272.0 21 1317.5 22 1501.0 23 1683.0 24 1884.5 25 2097.0 26 2412.0 27 2861.5 28 3197.0 29 3510.0 30 3920.5 31 4387.0 32 4961.5 33 5626.5 34 6244.0 35 6753.5 36 7483.0 37 7898.0 38 7986.5 39 7937.0 40 7765.5 41 7832.5 42 7878.0 43 7969.0 44 8016.0 45 7882.0 46 7518.5 47 7181.5 48 6898.0 49 6387.5 50 5936.0 51 5554.5 52 5088.5 53 4697.5 54 4268.5 55 3775.0 56 3309.0 57 2858.5 58 2538.5 59 2244.0 60 1772.5 61 1354.0 62 1107.0 63 839.5 64 584.0 65 412.5 66 293.0 67 227.0 68 154.5 69 99.5 70 73.5 71 52.5 72 44.5 73 31.0 74 29.5 75 28.0 76 19.5 77 15.5 78 9.0 79 4.5 80 3.5 81 2.0 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03473459209120057 2 0.0 3 0.0 4 0.0 5 0.013804773779836126 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0024492340577128608 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011132882080513002 24-25 0.0 26-27 0.0017812611328820805 28-29 0.003562522265764161 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.2265764161026006E-4 38-39 0.0 40-41 0.0 42-43 0.012023512646954044 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 4.453152832205201E-4 86-87 0.0 88-89 0.0 90-91 0.009351620947630923 92-93 8.906305664410402E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.012691485571784825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 224560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.0731958713804 #Duplication Level Percentage of deduplicated Percentage of total 1 78.82262819640128 44.198366699538006 2 11.908685600763821 13.355161205246343 3 3.5553955193118485 5.980871680638046 4 1.5873470717282068 3.5603049307551142 5 0.956233975453379 2.6809547502233038 6 0.649528506584919 2.1852683504268815 7 0.4607505194346032 1.808502788686774 8 0.31885578164843703 1.4303410159275929 9 0.24304522228292816 1.226549011920644 >10 1.4138966047217008 13.860753180181412 >50 0.05706722466816385 2.2046494655085307 >100 0.020662271000542083 1.935964708325684 >500 0.00196783533338496 0.6041269181010613 >1k 0.00393567066676992 4.9681852945206 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3114 1.3867117919486998 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2585 1.1511400071250444 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2015 0.8973102956893482 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1291 0.5749020306376915 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.2524937655860349 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 528 0.23512646954043462 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 418 0.1861417883861774 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 279 0.12424296401852511 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 263 0.11711791948699679 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 254 0.11311008193801211 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.2265764161026006E-4 0.0 20-21 0.0 0.0 0.0 8.906305664410402E-4 0.0 22-23 0.0 0.0 0.0 0.0020039187744923404 0.0 24-25 0.0 0.0 0.0 0.004453152832205202 0.0 26-27 0.0 0.0 0.0 0.005566441040256501 0.0 28-29 0.0 0.0 0.0 0.011800855005343783 0.0 30-31 0.0 0.0 0.0 0.026273601710010686 0.0 32-33 0.0 0.0 0.0 0.054996437477734236 0.0 34-35 0.0 0.0 0.0 0.09262557890986818 0.0 36-37 0.0 0.0 0.0 0.15363377271107945 0.0 38-39 0.0 0.0 0.0 0.2636266476665479 0.0 40-41 0.0 0.0 0.0 0.40523690773067333 0.0 42-43 0.0 0.0 0.0 0.5573120769504809 0.0 44-45 0.0 0.0 0.0 0.7465710723192021 0.0 46-47 0.0 0.0 0.0 1.0124242964018526 0.0 48-49 0.0 0.0 0.0 1.30967224795155 0.0 50-51 0.0 0.0 0.0 1.651451727823299 0.0 52-53 0.0 0.0 0.0 1.9874421090131813 0.0 54-55 0.0 0.0 0.0 2.3788742429640184 0.0 56-57 0.0 0.0 0.0 2.8711702885643033 0.0 58-59 0.0 0.0 0.0 3.3610171001068756 0.0 60-61 0.0 0.0 0.0 3.9394816530103314 0.0 62-63 0.0 0.0 0.0 4.615915568222301 0.0 64-65 0.0 0.0 0.0 5.305263626647667 0.0 66-67 0.0 0.0 0.0 6.0654168151050945 0.0 68-69 0.0 0.0 0.0 6.976309226932669 0.0 70-71 0.0 0.0 0.0 8.168418240114 0.0 72-73 0.0 0.0 0.0 9.303081581759887 0.0 74-75 0.0 0.0 0.0 10.432846455290345 0.0 76-77 0.0 0.0 0.0 11.818222301389383 0.0 78-79 0.0 0.0 0.0 13.264161026006413 0.0 80-81 0.0 0.0 0.0 14.771330602066264 0.0 82-83 0.0 0.0 0.0 16.22350374064838 0.0 84-85 0.0 0.0 0.0 17.717536515853226 0.0 86-87 0.0 0.0 0.0 19.115826505165657 0.0 88-89 0.0 0.0 0.0 20.85411471321696 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCAGT 20 0.0020721287 71.248 8 GGTATCA 1105 0.0 64.47782 1 ATCACGC 30 1.2991368E-4 63.331554 3 TATCACG 25 0.0050165886 56.998394 2 CACGCAG 45 1.512282E-5 52.77629 5 TCACGCA 40 5.3796126E-4 47.498665 4 GTATCAA 2055 0.0 42.066944 1 GGTACTC 50 0.001613271 37.998928 3 GTATCAC 50 0.001613271 37.998928 1 GTATAGT 50 0.001613271 37.998928 1 TAAGGTG 50 0.001613271 37.998928 5 GTGGTAC 50 0.001613271 37.998928 1 TGGTATC 390 0.0 37.75535 2 GTGGTAT 430 0.0 34.24322 1 TATCAAC 2770 0.0 31.037035 2 ATCAACG 2845 0.0 30.218834 3 TCAACGC 2870 0.0 29.955605 4 CAACGCA 2925 0.0 29.392336 5 AACGCAG 3015 0.0 28.514954 6 GCGCTTG 35 0.008387373 27.142096 68-69 >>END_MODULE