FastQCFastQC Report
Thu 26 May 2016
SRR937168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363473
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62301.7140200234955554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43551.1981632748512268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.8905750908595687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.2624679137102343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9440.25971667771746454No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7470.20551732865990044No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.15902144038209165No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.14801649641101267No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5130.14113840642908826No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.1273822264652395No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.10482209132452755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14000.061.772771
GATACCG250.00501940656.9974377
ATACCGT352.7860116E-454.283276
GTATCAA30450.049.3121031
GCGCAAG500.001611435138.0140151
TGCGTTC500.001614714437.998296
GGTATGG651.3267435E-436.536828
CCCTATA801.07489195E-535.6283072
TATCAAC42300.035.375632
ATCAACG42700.035.155493
TCAACGC43300.034.673134
CCTTTAG1106.967457E-834.5581971
CAACGCA44250.033.9287345
AACGCAG44800.033.5029566
TGGGATA2000.030.8736116
TGGTATC5450.030.5074212
GTAAGGT953.4788398E-530.0069244
GTGGTAT5500.029.3744661
ACGCAGA51150.029.2508837
CGCAGAG51350.029.0444588
Produced by FastQC (version 0.11.5)