##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937168_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 363473 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26416267508178 33.0 31.0 34.0 31.0 34.0 2 32.37221471746182 34.0 31.0 34.0 31.0 34.0 3 32.38135707466579 34.0 31.0 34.0 31.0 34.0 4 35.992733985743094 37.0 35.0 37.0 35.0 37.0 5 36.0277407125151 37.0 35.0 37.0 35.0 37.0 6 36.09072475809757 37.0 37.0 37.0 35.0 37.0 7 35.97747012845521 37.0 37.0 37.0 35.0 37.0 8 36.01953927802065 37.0 37.0 37.0 35.0 37.0 9 37.7312400095743 39.0 38.0 39.0 35.0 39.0 10-11 37.658394158575746 39.0 38.0 39.0 35.0 39.0 12-13 37.54671048468525 39.0 38.0 39.0 35.0 39.0 14-15 38.756173085758775 40.0 38.0 41.0 35.5 41.0 16-17 38.73918282788543 40.0 38.0 41.0 35.5 41.0 18-19 38.820271381918324 40.0 38.5 41.0 36.0 41.0 20-21 38.79014672341549 40.0 38.5 41.0 35.5 41.0 22-23 38.642457624087626 40.0 38.0 41.0 35.0 41.0 24-25 38.57980785367826 40.0 38.0 41.0 35.0 41.0 26-27 38.539913556165104 40.0 38.0 41.0 35.0 41.0 28-29 38.42137655341662 40.0 38.0 41.0 35.0 41.0 30-31 38.09825489100979 40.0 37.5 41.0 34.5 41.0 32-33 37.730340355404664 40.0 36.5 41.0 34.0 41.0 34-35 37.7047951292118 40.0 37.0 41.0 34.0 41.0 36-37 37.570721896812145 40.0 36.5 41.0 33.5 41.0 38-39 37.42448957694244 40.0 36.0 41.0 33.0 41.0 40-41 37.15662511383239 40.0 36.0 41.0 33.0 41.0 42-43 36.79497101572882 40.0 35.5 41.0 32.5 41.0 44-45 36.374267139512426 40.0 35.0 41.0 31.0 41.0 46-47 35.77376448869654 40.0 35.0 41.0 29.0 41.0 48-49 35.24273604917009 39.0 35.0 41.0 25.0 41.0 50-51 34.17333061878048 38.0 34.0 40.0 22.0 40.5 52-53 34.23723632842054 38.5 35.0 40.0 22.0 40.5 54-55 34.56459764549224 39.0 35.0 40.5 20.0 41.0 56-57 34.35851631345382 39.0 35.0 41.0 16.5 41.0 58-59 34.10344647332814 38.5 35.0 40.0 11.0 41.0 60-61 33.8302501148641 38.0 34.5 40.0 7.5 41.0 62-63 33.52681354598553 38.0 34.0 40.0 2.0 41.0 64-65 33.115985231365194 37.5 34.0 40.0 2.0 41.0 66-67 32.767971761313774 37.0 33.5 40.0 2.0 41.0 68-69 32.30396343057119 36.0 33.0 39.0 2.0 41.0 70-71 31.838323616884885 36.0 33.0 39.0 2.0 40.5 72-73 31.385636622252548 35.0 32.0 38.5 2.0 40.0 74-75 30.901361311569225 35.0 31.5 38.0 2.0 40.0 76-77 30.43658676160265 35.0 31.0 37.0 2.0 39.0 78-79 29.944035733053074 35.0 31.0 36.5 2.0 39.0 80-81 29.45623608906301 35.0 30.0 36.0 2.0 38.5 82-83 28.988118787365224 34.0 29.5 36.0 2.0 37.0 84-85 28.484949363501556 34.0 29.0 35.0 2.0 37.0 86-87 28.043259059132314 34.0 29.0 35.0 2.0 36.5 88-89 27.60571899425817 34.0 27.0 35.0 2.0 36.0 90-91 27.14385525197195 34.0 26.5 35.0 2.0 36.0 92-93 26.69157406464854 34.0 25.0 35.0 2.0 35.0 94-95 26.207659165880273 33.0 24.5 35.0 2.0 35.0 96-97 25.603167222874877 33.0 21.5 35.0 2.0 35.0 98-99 24.8245055341112 33.0 9.5 35.0 2.0 35.0 100-101 22.575189904064402 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17672646564943761 1101 2 0.1332469997204555 1101 3 0.05650251589595712 1101 4 0.09778838114911537 1101 5 0.1244445510894181 1101 6 0.0840713162686697 1101 7 0.029584603419472444 1101 8 0.028042283363042486 1101 9 -0.056458935518385545 1101 10-11 0.10980212157048896 1101 12-13 0.1457490323157984 1101 14-15 0.12721715839344938 1101 16-17 0.15856210821589656 1101 18-19 0.1595755628275981 1101 20-21 0.21470508635859886 1101 22-23 0.049722857923491404 1101 24-25 0.12691793336578172 1101 26-27 0.20609132180761236 1101 28-29 0.08524797780172122 1101 30-31 0.16545015028169274 1101 32-33 0.08087734632150756 1101 34-35 0.21578107885453335 1101 36-37 0.34111510181680416 1101 38-39 0.3563426412521764 1101 40-41 0.1815847387369942 1101 42-43 0.4364435686692758 1101 44-45 0.4230250219629781 1101 46-47 0.639009714911758 1101 48-49 0.6607884675999927 1101 50-51 0.7898099193897394 1101 52-53 1.003518060645078 1101 54-55 1.1325720316753447 1101 56-57 1.2188405202280777 1101 58-59 1.4052596900335104 1101 60-61 1.6218013504586324 1101 62-63 1.412798138080639 1101 64-65 1.4964145988397703 1101 66-67 1.6588581054435743 1101 68-69 1.5611393961613231 1101 70-71 1.4599074421802776 1101 72-73 1.4900247999591905 1101 74-75 1.7399353430868807 1101 76-77 1.8341957119312617 1101 78-79 1.711042641671252 1101 80-81 2.0245785267488223 1101 82-83 1.9981184582248765 1101 84-85 1.8778010602441988 1101 86-87 1.9280339948319138 1101 88-89 1.8540225771246561 1101 90-91 1.966063638983158 1101 92-93 2.395378576253801 1101 94-95 2.187452151392634 1101 96-97 2.1439365786393516 1101 98-99 2.2564167934408204 1101 100-101 2.2451617431413986 1102 1 0.2051086249548959 1102 2 0.07863000763399697 1102 3 0.21316853040271155 1102 4 0.10484731199895947 1102 5 0.12182070772302467 1102 6 0.16279021814341377 1102 7 0.20405818434151968 1102 8 0.2282225675687286 1102 9 0.22281594268174842 1102 10-11 0.17218021667941485 1102 12-13 0.12320690498617637 1102 14-15 0.22833415228035392 1102 16-17 0.2215157444475011 1102 18-19 0.2604873063871125 1102 20-21 0.19051795567416008 1102 22-23 0.08856558215946819 1102 24-25 0.28924873687881103 1102 26-27 0.15295969393245912 1102 28-29 0.11055138219647631 1102 30-31 0.19665900269230008 1102 32-33 0.24163456399128336 1102 34-35 0.48321385337084877 1102 36-37 0.5818485296533069 1102 38-39 0.6988118146935065 1102 40-41 0.6643587149159558 1102 42-43 0.633105790246006 1102 44-45 0.804126267988508 1102 46-47 0.8093278769250816 1102 48-49 1.032415790619595 1102 50-51 1.1472449624623025 1102 52-53 1.3520205134465044 1102 54-55 1.58051226662095 1102 56-57 1.5541253320639399 1102 58-59 1.3839940757048694 1102 60-61 1.6211345869682177 1102 62-63 1.6956720987758018 1102 64-65 1.7277803297169854 1102 66-67 1.3906007287348068 1102 68-69 1.631747309221602 1102 70-71 1.5825469133786179 1102 72-73 1.6585635256547313 1102 74-75 1.5259570756156968 1102 76-77 1.569431791818051 1102 78-79 1.4759211898912774 1102 80-81 1.5371426074614156 1102 82-83 1.6163906585684025 1102 84-85 1.5468901208818657 1102 86-87 1.5633011623413857 1102 88-89 1.5051678379426114 1102 90-91 1.6577002282748516 1102 92-93 1.8564236244996302 1102 94-95 2.1336543601742264 1102 96-97 2.052043246394284 1102 98-99 2.2049673780441665 1102 100-101 2.2975449875129215 1103 1 -0.08840395116637723 1103 2 -0.04483459409146917 1103 3 -0.18862958878671066 1103 4 -0.09094714466505849 1103 5 -0.04440906720240889 1103 6 -0.18476804245833023 1103 7 -0.11590224431456164 1103 8 -0.08863442320130588 1103 9 -0.013627858979447183 1103 10-11 -0.04625644740942647 1103 12-13 0.015381688465303966 1103 14-15 0.09315716927728346 1103 16-17 -0.08115063430583547 1103 18-19 0.0017243628415641865 1103 20-21 -0.07932714025789522 1103 22-23 -0.0023907587968707844 1103 24-25 -0.08363836426916293 1103 26-27 -0.004401680820215859 1103 28-29 0.008121192809760203 1103 30-31 -0.10781185612638211 1103 32-33 0.08032442442027588 1103 34-35 0.3255469891822429 1103 36-37 0.547460462656538 1103 38-39 0.5249037929593996 1103 40-41 0.6505112554163262 1103 42-43 0.6843468306174856 1103 44-45 0.7749030648522606 1103 46-47 1.0974052519589819 1103 48-49 1.0783019778101277 1103 50-51 1.121124360254747 1103 52-53 1.1564620787576345 1103 54-55 1.235131769066541 1103 56-57 1.1372787195651526 1103 58-59 1.1383228191640526 1103 60-61 1.519541354940209 1103 62-63 1.4569969687963251 1103 64-65 1.3879371420477113 1103 66-67 1.3805405012832743 1103 68-69 1.3776646312258833 1103 70-71 1.4406759454206899 1103 72-73 1.5908486535920296 1103 74-75 1.775147737849835 1103 76-77 1.9092657729563811 1103 78-79 1.578887031987552 1103 80-81 1.6743425164004542 1103 82-83 1.73191420887456 1103 84-85 1.4504935570939956 1103 86-87 1.874583180145141 1103 88-89 2.0176234373547324 1103 90-91 2.0855427865521925 1103 92-93 1.887975243007773 1103 94-95 1.773611206652813 1103 96-97 2.1983710122872644 1103 98-99 2.616637409279413 1103 100-101 2.822153207773315 1104 1 -0.08299084575326532 1104 2 -0.15936450862138685 1104 3 -0.03164953180665009 1104 4 -0.07271352643144269 1104 5 0.10772768493434626 1104 6 -0.15342901111930018 1104 7 -0.07744070585302865 1104 8 -0.1595745941414819 1104 9 -0.1264483717999596 1104 10-11 -0.04953280068578181 1104 12-13 -0.0011425280589136833 1104 14-15 0.08489506101517463 1104 16-17 -0.017760320915520822 1104 18-19 -0.02177991066271545 1104 20-21 0.09873553780478517 1104 22-23 -0.15922836563447618 1104 24-25 0.09285736222656027 1104 26-27 -0.02064099705953737 1104 28-29 -0.030910146221579282 1104 30-31 -0.15781185612638637 1104 32-33 -0.05870691461106503 1104 34-35 0.10489171852697154 1104 36-37 0.23364279883887917 1104 38-39 0.3966986647542754 1104 40-41 0.6475198024248741 1104 42-43 0.5212414175120728 1104 44-45 0.403393093342288 1104 46-47 0.3324479870017214 1104 48-49 0.7274472769554308 1104 50-51 0.6916371807675645 1104 52-53 0.7618751841707407 1104 54-55 0.9223112562460258 1104 56-57 0.7854268677133049 1104 58-59 0.9079809388221705 1104 60-61 0.7594273948262469 1104 62-63 1.0282220400213973 1104 64-65 1.148905803016369 1104 66-67 1.2158681366109114 1104 68-69 1.2456133491745973 1104 70-71 1.2913881961329459 1104 72-73 1.2278856906290692 1104 74-75 1.3731534358555315 1104 76-77 1.3661033797939837 1104 78-79 1.154100707201227 1104 80-81 1.1126616047195412 1104 82-83 1.2202332971936478 1104 84-85 1.436675893276334 1104 86-87 1.5020760576380212 1104 88-89 1.3233214430527376 1104 90-91 1.6487906498000555 1104 92-93 1.7691718242043528 1104 94-95 1.3649217479633542 1104 96-97 1.4087698726861255 1104 98-99 1.7025348451768494 1104 100-101 1.7885349741550804 1105 1 0.1457255594868414 1105 2 0.07976265465927668 1105 3 0.12106602452064408 1105 4 0.07880969326333087 1105 5 0.05283003680870735 1105 6 0.1124736340158563 1105 7 0.14202254972761352 1105 8 -0.060321251547279076 1105 9 -0.015344978078054794 1105 10-11 -0.1063925950173612 1105 12-13 0.06888892030210769 1105 14-15 -0.19305828736706587 1105 16-17 0.015916436237539244 1105 18-19 0.013015867925389557 1105 20-21 0.13625169608844345 1105 22-23 0.01892370551985323 1105 24-25 0.01815882274247116 1105 26-27 0.2552016453745267 1105 28-29 0.1532113254845413 1105 30-31 0.06872824535729194 1105 32-33 0.3272648011574901 1105 34-35 0.5276059243111604 1105 36-37 0.27620227096945626 1105 38-39 0.38873046039780235 1105 40-41 0.5610040107172054 1105 42-43 0.7208806295666363 1105 44-45 0.3810879091860855 1105 46-47 0.3531788237596416 1105 48-49 0.7137759219748219 1105 50-51 1.025472144399366 1105 52-53 1.0761851531082485 1105 54-55 1.3250393533307872 1105 56-57 1.3065146337220597 1105 58-59 1.2179710991509296 1105 60-61 1.537924819147598 1105 62-63 1.4526012725270405 1105 64-65 1.2100545363998734 1105 66-67 0.9700557849294889 1105 68-69 1.2801505952242636 1105 70-71 1.3349123419144284 1105 72-73 1.5017449640368739 1105 74-75 1.4181380975444853 1105 76-77 1.4062125808512675 1105 78-79 1.238212532170838 1105 80-81 1.364429851521649 1105 82-83 1.5601283407004338 1105 84-85 1.4545435786383756 1105 86-87 1.596421140696421 1105 88-89 1.9655278446270863 1105 90-91 1.9475172805808647 1105 92-93 2.186773259638745 1105 94-95 1.9991213582351968 1105 96-97 2.277051403044407 1105 98-99 2.8459355127310175 1105 100-101 2.8728050056034427 1106 1 -0.20253713539301543 1106 2 -0.07774457092668285 1106 3 -0.04989380051821257 1106 4 0.2705567028200946 1106 5 0.13843301283593945 1106 6 0.030850883440969312 1106 7 -0.20308971935250497 1106 8 -0.20841236073625424 1106 9 -0.3392129696112818 1106 10-11 -0.6298027903015608 1106 12-13 -0.2293396684055864 1106 14-15 -0.35622643431192813 1106 16-17 -0.17631931125021083 1106 18-19 -0.41462278948689857 1106 20-21 -0.39271745364820987 1106 22-23 -0.0850051848692388 1106 24-25 -0.12767143400316883 1106 26-27 -0.48974396055502467 1106 28-29 -0.18960493015000424 1106 30-31 -0.4078158500556128 1106 32-33 -0.25215687067784387 1106 34-35 -0.041541702672802217 1106 36-37 -0.4664051283118553 1106 38-39 -0.09375584439182916 1106 40-41 -0.15799980776763078 1106 42-43 -0.07181713349040564 1106 44-45 0.17442911858392307 1106 46-47 0.05151500513415641 1106 48-49 0.38661334453271223 1106 50-51 0.22330903954222237 1106 52-53 0.0671311950342286 1106 54-55 -0.008497913815475044 1106 56-57 -0.15722030857873648 1106 58-59 0.03517959686195127 1106 60-61 -0.08770895791758448 1106 62-63 -0.12442593505648603 1106 64-65 0.13353716334867016 1106 66-67 0.12286550061762469 1106 68-69 -0.22448010876932756 1106 70-71 0.13966681263585912 1106 72-73 -0.055156085870653015 1106 74-75 0.18391308119462124 1106 76-77 0.10187300995613668 1106 78-79 -0.06317703063725943 1106 80-81 0.05627531188465085 1106 82-83 0.5324706963469374 1106 84-85 0.42931907563913896 1106 86-87 0.22373912976838284 1106 88-89 0.48150500404003793 1106 90-91 0.6557161230806692 1106 92-93 0.8541466624502227 1106 94-95 1.040998111890186 1106 96-97 1.3057744257654456 1106 98-99 1.7647522746839996 1106 100-101 1.7870332567655147 1107 1 -6.953634577868684E-4 1107 2 -0.0692546185114935 1107 3 0.06554216538504676 1107 4 -0.1418221955401151 1107 5 -0.12499514778848919 1107 6 -0.17907003676032218 1107 7 -0.03958966800198738 1107 8 -0.026973861540746213 1107 9 0.05474820939662095 1107 10-11 0.1056564545034746 1107 12-13 -0.02837085528724259 1107 14-15 0.01969359581370611 1107 16-17 -0.1838164869716863 1107 18-19 -0.10663949552230179 1107 20-21 0.22449866356790693 1107 22-23 -0.12968013608624318 1107 24-25 -0.06623909686990004 1107 26-27 0.0499328735143294 1107 28-29 -0.09891991423133817 1107 30-31 0.24902575071122612 1107 32-33 0.27487061896646736 1107 34-35 0.3073337209689768 1107 36-37 0.32411898931506755 1107 38-39 0.5273457954014091 1107 40-41 0.8443451992502631 1107 42-43 0.6323525286231799 1107 44-45 0.4743332642824569 1107 46-47 0.47469462924835426 1107 48-49 0.5798282293363783 1107 50-51 0.5353490244794088 1107 52-53 0.5097384320339913 1107 54-55 0.6185261524609231 1107 56-57 0.6907992730857089 1107 58-59 0.21494064578187988 1107 60-61 0.7645555999544555 1107 62-63 0.7846322964316599 1107 64-65 0.8559876100981754 1107 66-67 0.6971868179295946 1107 68-69 0.26685877042002204 1107 70-71 0.1596421643869128 1107 72-73 0.3525499152932916 1107 74-75 0.38707284977494893 1107 76-77 0.1775807912713958 1107 78-79 0.21747067057119196 1107 80-81 0.5108301028880398 1107 82-83 0.6822601592205118 1107 84-85 0.8022436588440982 1107 86-87 0.6779002334621964 1107 88-89 0.6918196115509083 1107 90-91 0.6942118952213043 1107 92-93 1.1156919707245017 1107 94-95 1.2895859726275773 1107 96-97 1.279465843382095 1107 98-99 1.5449035875455905 1107 100-101 1.85471324033335 1108 1 0.3031109751311618 1108 2 0.40730215804528314 1108 3 0.010230810073686314 1108 4 0.3134254553597131 1108 5 0.14555748798154156 1108 6 0.21390655186844043 1108 7 0.2945399704754763 1108 8 0.1792213924805921 1108 9 0.10201688275225251 1108 10-11 0.1668923200871646 1108 12-13 0.2708567287229542 1108 14-15 0.29648926391372754 1108 16-17 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-0.04397269948757909 2316 82-83 0.11045817479717002 2316 84-85 0.278274100893956 2316 86-87 0.04775691399848725 2316 88-89 -0.21840123556314595 2316 90-91 -0.29815981074263576 2316 92-93 -0.5589586214018176 2316 94-95 -0.13103709362655636 2316 96-97 -0.4383050588548265 2316 98-99 -0.9245832363101627 2316 100-101 -0.9900892064108469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1491.0 3 298.0 4 172.0 5 185.0 6 167.0 7 216.0 8 259.0 9 319.0 10 414.0 11 646.0 12 1187.0 13 1862.0 14 2068.0 15 2999.0 16 4679.0 17 7133.0 18 6023.0 19 3107.0 20 2750.0 21 2492.0 22 2502.0 23 2689.0 24 2796.0 25 3014.0 26 3336.0 27 3710.0 28 4141.0 29 5004.0 30 6089.0 31 7816.0 32 10398.0 33 14384.0 34 20835.0 35 33290.0 36 49026.0 37 74702.0 38 70450.0 39 10824.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.981788499509136 20.784385099881973 16.139128804173975 20.094697596434912 2 14.52119828627198 21.80068262396683 42.0026025727976 21.67551651696359 3 17.268053315690086 28.67331045224607 31.974527560683125 22.084108671380722 4 11.071503402035708 18.23397404086854 39.7663237061788 30.92819885091695 5 11.415622044986504 38.962757525549236 34.70919221130811 14.912428218156155 6 26.782434601320382 39.499533949953396 18.712323025078454 15.005708423647764 7 24.740707239790304 36.465896900620756 22.110412800141194 16.682983059447743 8 25.782755796735795 33.97029181522137 20.773945916434357 19.47300647160847 9 27.272326216847404 16.1227972945773 21.60036836982566 35.00450811874964 10-11 23.04279123382264 28.52041111794408 27.845188226826355 20.591609421406922 12-13 25.190591291470582 26.858308180844215 28.773666205671756 19.177434322013447 14-15 22.154573266989562 26.71122673935253 29.357946155659693 21.776253837998215 16-17 18.686755177819727 30.796117789969625 29.54966308494782 20.967463947262832 18-19 18.445808165690753 27.303209830925834 35.267053907125714 18.983928096257703 20-21 19.822707501843926 26.23942759062804 35.482901475829074 18.45496343169896 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28.39446764681956 33.367711761696825 19.487329389006163 48-49 18.72180305988506 28.62920127513595 32.75795029617129 19.891045368807703 50-51 18.84600906988833 28.889207549875422 32.22788611485308 20.036897265383168 52-53 19.109300016548072 29.08075459209002 31.938606652325003 19.871338739036904 54-55 18.752231998201992 29.010392321512533 31.912851759469536 20.324523920815942 56-57 18.98600853907862 29.291769315288313 31.639395564476665 20.0828265811564 58-59 18.991433317022405 28.695286601377013 31.981364190851636 20.33191589074895 60-61 18.977532736044108 28.72074431426602 32.241075120606475 20.060647829083393 62-63 18.96269380913522 28.46223616594252 32.56655124501003 20.008518779912222 64-65 18.954506922859565 28.360498693995133 32.43013390857277 20.254860474572535 66-67 18.874621294614183 28.576370989306653 32.15818651464236 20.390821201436808 68-69 18.75639240768054 28.327980481618813 32.16232235654128 20.75330475415937 70-71 18.850314555569646 28.32543957831358 32.24870014942079 20.57554571669598 72-73 19.038068417562997 28.20318778370048 31.93132053312602 20.827423265610502 74-75 18.661207835352826 28.475524861116853 31.794521869787573 21.068745433742745 76-77 18.45610658608006 28.17006457590012 32.13037052559599 21.24345831242383 78-79 18.680709687202746 28.009484553136932 31.92641199906257 21.383393760597748 80-81 18.422685102218058 28.483891868047035 31.668435781144456 21.42498724859045 82-83 18.68211091628897 28.129790143469968 31.545748282440645 21.64235065780042 84-85 18.562393859861935 28.06179837233409 31.408934518427028 21.966873249376945 86-87 18.515950356144895 28.028265511636913 31.495146862382086 21.960637269836106 88-89 18.76887660507144 27.76223920819922 31.399918669487963 22.06896551724138 90-91 18.787007332880226 27.921054699509074 31.26131330106443 22.030624666546267 92-93 18.9384561517657 27.962709219584415 30.966428372532857 22.132406256117026 94-95 18.911262864499907 27.672356040284185 30.83096467552375 22.585416419692162 96-97 19.360104112671088 27.785069095962328 30.36555238168764 22.48927440967895 98-99 19.60800262489626 27.935982309522693 29.960488693921107 22.495526371659942 100-101 19.957821900909035 27.347672968111425 30.158030034665988 22.536475096313552 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 924.0 1 608.5 2 388.5 3 483.5 4 393.5 5 378.0 6 723.0 7 1614.0 8 2669.5 9 2967.0 10 2219.5 11 1536.0 12 1454.5 13 1473.0 14 1483.0 15 1456.0 16 1490.0 17 1537.0 18 1549.5 19 1616.0 20 1696.5 21 1846.0 22 2121.0 23 2426.0 24 2688.5 25 3056.0 26 3660.5 27 4303.5 28 4764.5 29 5224.0 30 6052.5 31 6847.5 32 7665.5 33 8887.5 34 10231.5 35 11058.0 36 11915.0 37 12802.0 38 12712.0 39 12354.5 40 12223.5 41 12422.0 42 12562.0 43 12596.0 44 12643.0 45 12626.0 46 12116.5 47 11473.0 48 11407.0 49 10879.0 50 9967.5 51 9065.5 52 8273.5 53 7871.0 54 7528.5 55 6917.5 56 6136.5 57 5233.0 58 4491.5 59 3943.5 60 3308.5 61 2759.0 62 2208.5 63 1581.5 64 1113.5 65 860.5 66 627.0 67 494.0 68 394.0 69 240.0 70 163.5 71 118.0 72 95.0 73 87.5 74 75.5 75 55.0 76 33.0 77 20.0 78 11.5 79 6.5 80 7.5 81 9.0 82 5.5 83 2.5 84 2.5 85 1.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23247944138904403 2 0.20771831745411626 3 0.20028998027363792 4 0.20606757585845442 5 0.20414171066351558 6 0.23413018298470586 7 0.23440530658398284 8 0.22340036261290383 9 0.2184481378259183 10-11 0.23440530658398284 12-13 0.23426774478434437 14-15 0.22463841880965024 16-17 0.23234187958940553 18-19 0.22601403680603513 20-21 0.21886082322483377 22-23 0.21789789062736437 24-25 0.21129492424471694 26-27 0.21638471083134098 28-29 0.2240881716110963 30-31 0.2240881716110963 32-33 0.2205115648204956 34-35 0.2316540705912131 36-37 0.21487153103531761 38-39 0.2151466546345946 40-41 0.21404616023748668 42-43 0.21638471083134098 44-45 0.2294530817969973 46-47 0.2317916323908516 48-49 0.23247944138904403 50-51 0.23234187958940553 52-53 0.24568537415433886 54-55 0.2337174975857904 56-57 0.21665983443061795 58-59 0.20097778927183038 60-61 0.19891436227725307 62-63 0.2049670814613465 64-65 0.1996021712754455 66-67 0.19918948587653004 68-69 0.20304121626640767 70-71 0.20331633986568465 72-73 0.204829519661708 74-75 0.2085436882519472 76-77 0.21982375582230315 78-79 0.21404616023748668 80-81 0.21129492424471694 82-83 0.20606757585845442 84-85 0.2049670814613465 86-87 0.21170760964363242 88-89 0.20785587925375476 90-91 0.21776032882772586 92-93 0.20799344105339324 94-95 0.2063426994577314 96-97 0.21707251982953343 98-99 0.21789789062736437 100-101 0.1996021712754455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 363473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.46403010714619 #Duplication Level Percentage of deduplicated Percentage of total 1 81.6064854621252 47.71044022996076 2 10.304253811381967 12.048564101206223 3 3.126242780180037 5.483182560680824 4 1.4129700565210468 3.3043169569977016 5 0.833572321455015 2.4366998649014873 6 0.5031075705932463 1.7648217692578037 7 0.3703441145884183 1.5156266625711179 8 0.26320802325123255 1.2310561436641994 9 0.21028529165060306 1.1064713059935787 >10 1.2716941771085315 13.608699931149381 >50 0.0724365588283019 2.8222804533789803 >100 0.021166526930342502 1.991518566049537 >500 0.0028222035907123338 1.1753314486738085 >1k 9.407345302374447E-4 2.087767046872146 >5k 4.7036726511872235E-4 1.7132229586424488 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6230 1.7140200234955554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4355 1.1981632748512268 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3237 0.8905750908595687 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 954 0.2624679137102343 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 944 0.25971667771746454 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 747 0.20551732865990044 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 578 0.15902144038209165 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 538 0.14801649641101267 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 513 0.14113840642908826 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 463 0.1273822264652395 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 381 0.10482209132452755 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.3756179963848758E-4 0.0 12-13 0.0 0.0 0.0 2.7512359927697516E-4 0.0 14-15 0.0 0.0 0.0 2.7512359927697516E-4 0.0 16-17 0.0 0.0 0.0 5.502471985539503E-4 0.0 18-19 0.0 0.0 0.0 8.253707978309255E-4 0.0 20-21 0.0 0.0 0.0 8.253707978309255E-4 0.0 22-23 0.0 0.0 0.0 0.0015131797960233635 0.0 24-25 0.0 0.0 0.0 0.002888797792408239 0.0 26-27 0.0 0.0 0.0 0.004677101187708578 0.0 28-29 0.0 0.0 0.0 0.009766887774332619 0.0 30-31 0.0 0.0 0.0 0.021322078943965577 0.0 32-33 0.0 0.0 0.0 0.042506596088292666 0.0 34-35 0.0 0.0 0.0 0.06671747282466649 0.0 36-37 0.0 0.0 0.0 0.09973230473790351 0.0 38-39 0.0 0.0 0.0 0.16686246296148544 0.0 40-41 0.0 0.0 0.0 0.2551771383293945 0.0 42-43 0.0 0.0 0.0 0.3506450272785049 0.0 44-45 0.0 0.0 0.0 0.4650964445777266 0.0 46-47 0.0 0.0 0.0 0.6100865813966925 0.0 48-49 0.0 0.0 0.0 0.7730973139683003 0.0 50-51 0.0 0.0 0.0 0.969948249250976 0.0 52-53 0.0 0.0 0.0 1.1773914431058152 0.0 54-55 0.0 0.0 0.0 1.4195002104695535 0.0 56-57 0.0 0.0 0.0 1.7284640124575965 0.0 58-59 0.0 0.0 0.0 2.0388034324420246 0.0 60-61 0.0 0.0 0.0 2.400866089090524 0.0 62-63 0.0 0.0 0.0 2.804609971029485 0.0 64-65 0.0 0.0 0.0 3.230088617311327 0.0 66-67 0.0 0.0 0.0 3.6900952753024296 0.0 68-69 0.0 0.0 0.0 4.270468507977209 0.0 70-71 0.0 0.0 0.0 5.016328585617089 0.0 72-73 0.0 0.0 0.0 5.729173831343731 0.0 74-75 0.0 0.0 0.0 6.426199470111948 0.0 76-77 0.0 0.0 0.0 7.2928388078344195 0.0 78-79 0.0 0.0 0.0 8.19235541567049 0.0 80-81 0.0 0.0 0.0 9.145796248964848 0.0 82-83 0.0 0.0 0.0 10.07158716053187 0.0 84-85 0.0 0.0 0.0 11.03204364560779 0.0 86-87 0.0 0.0 0.0 11.939263714223616 0.0 88-89 0.0 0.0 0.0 13.024626313371282 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1400 0.0 61.77277 1 GATACCG 25 0.005019406 56.997437 7 ATACCGT 35 2.7860116E-4 54.28327 6 GTATCAA 3045 0.0 49.312103 1 GCGCAAG 50 0.0016114351 38.014015 1 TGCGTTC 50 0.0016147144 37.99829 6 GGTATGG 65 1.3267435E-4 36.53682 8 CCCTATA 80 1.07489195E-5 35.628307 2 TATCAAC 4230 0.0 35.37563 2 ATCAACG 4270 0.0 35.15549 3 TCAACGC 4330 0.0 34.67313 4 CCTTTAG 110 6.967457E-8 34.558197 1 CAACGCA 4425 0.0 33.928734 5 AACGCAG 4480 0.0 33.502956 6 TGGGATA 200 0.0 30.873611 6 TGGTATC 545 0.0 30.507421 2 GTAAGGT 95 3.4788398E-5 30.006924 4 GTGGTAT 550 0.0 29.374466 1 ACGCAGA 5115 0.0 29.250883 7 CGCAGAG 5135 0.0 29.044458 8 >>END_MODULE