FastQCFastQC Report
Thu 26 May 2016
SRR937168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363473
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51971.42981734544244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42751.176153386909069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32070.8823213828812595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14630.40250582574221466No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9620.26466890250445013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.2404580257680763No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5640.15516970999221402No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.11857827128837631No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.11114993410789797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14050.065.6403351
GTATCAA29000.048.5220451
CGGGTCG405.380059E-447.5057755
TATCAAC40450.034.9980772
GTCGGGA550.002575777234.5496568
ATCAACG40600.034.5177463
GGCCCGC702.0491141E-433.93279
TCAACGC41350.033.891674
GTGGTAT5150.033.2307851
CAACGCA42300.033.130515
TGGTATC5200.032.888622
AACGCAG43300.032.3564646
CTAGACA753.0721427E-431.670524
GGTCGGG600.003945077331.6705197
TGCGGGA300.00395297431.66180846-47
ACGAATC404.496074E-429.68294172-73
TCTAGAC650.005835413529.2343253
ACGCAGA50850.027.2796237
CGCAGAG50750.027.2397678
CACTAGG700.00835108627.1648521