##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937168_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 363473 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.444484184519897 31.0 31.0 34.0 30.0 34.0 2 31.688084121791714 31.0 31.0 34.0 30.0 34.0 3 31.665111301252086 31.0 31.0 34.0 30.0 34.0 4 35.54281335890148 37.0 35.0 37.0 33.0 37.0 5 35.422490803993696 37.0 35.0 37.0 33.0 37.0 6 35.50408420983127 37.0 35.0 37.0 33.0 37.0 7 35.40758460738487 37.0 35.0 37.0 33.0 37.0 8 35.38610295675332 37.0 35.0 37.0 33.0 37.0 9 36.922417896239885 39.0 37.0 39.0 33.0 39.0 10-11 36.80411062169679 39.0 37.0 39.0 32.0 39.0 12-13 36.67454941632528 38.0 35.0 39.0 32.0 39.0 14-15 37.49675079029254 39.0 36.0 40.5 32.0 41.0 16-17 37.47162100073458 39.0 36.0 40.0 32.0 41.0 18-19 37.60407650637049 39.0 36.5 40.0 32.0 41.0 20-21 37.55392147422229 39.0 36.0 40.0 32.0 41.0 22-23 37.35646251578522 39.0 36.0 40.0 32.0 41.0 24-25 37.23147661586968 39.0 36.0 40.0 31.5 41.0 26-27 37.06132917713283 39.0 36.0 40.0 31.0 41.0 28-29 36.84641225070363 39.0 35.5 40.0 31.0 41.0 30-31 36.73980873407378 38.5 35.0 40.0 31.0 41.0 32-33 36.50506640108068 38.0 35.0 40.0 30.5 41.0 34-35 36.26146233695488 38.0 35.0 40.0 30.0 41.0 36-37 35.930576686576444 38.0 35.0 40.0 30.0 41.0 38-39 35.70647475878539 38.0 35.0 40.0 30.0 41.0 40-41 35.370929890253194 38.0 34.5 40.0 28.5 41.0 42-43 34.977892443180096 38.0 34.0 40.0 26.5 41.0 44-45 34.39410492663829 38.0 33.0 40.0 24.5 41.0 46-47 33.68485141950021 38.0 33.0 40.0 21.5 41.0 48-49 33.068807036561175 37.0 33.0 40.0 16.5 41.0 50-51 32.66680193576964 37.0 32.0 40.0 10.5 41.0 52-53 32.309453521994755 37.0 31.5 40.0 8.0 41.0 54-55 31.99167888673987 36.5 31.0 39.5 6.5 40.5 56-57 31.629325974694133 36.0 31.0 39.0 2.0 40.0 58-59 31.253735490669186 35.5 30.0 39.0 2.0 40.0 60-61 30.827215501564076 35.0 29.5 39.0 2.0 40.0 62-63 30.3446720939382 35.0 28.5 38.5 2.0 40.0 64-65 29.853210554841763 35.0 28.0 38.0 2.0 40.0 66-67 29.3365298110176 34.0 27.5 38.0 2.0 40.0 68-69 28.787183642251282 34.0 26.0 37.0 2.0 39.0 70-71 28.323429800838028 34.0 26.0 36.5 2.0 39.0 72-73 27.759423671084235 33.0 25.5 36.0 2.0 39.0 74-75 27.1725272578706 32.5 24.5 35.5 2.0 38.5 76-77 26.20070404129055 31.5 23.0 34.5 2.0 36.5 78-79 26.743689352441585 32.0 24.5 35.0 2.0 37.0 80-81 26.469744657787512 32.0 24.0 35.0 2.0 37.0 82-83 26.01853370126529 32.0 22.5 35.0 2.0 36.0 84-85 25.497759118283888 32.0 20.5 35.0 2.0 36.0 86-87 24.914472326692767 31.0 18.5 34.0 2.0 35.5 88-89 24.23518665760593 31.0 13.0 34.0 2.0 35.0 90-91 23.459576089558233 30.5 4.5 34.0 2.0 35.0 92-93 22.810361429872373 30.0 2.0 34.0 2.0 35.0 94-95 22.081337540890246 29.0 2.0 34.0 2.0 35.0 96-97 21.290084820605657 29.0 2.0 34.0 2.0 35.0 98-99 20.124504433616803 28.5 2.0 34.0 2.0 35.0 100-101 17.756274881490512 22.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.061028339199811654 1101 2 -0.04577188118520681 1101 3 -0.03275801508060994 1101 4 0.03085535259920391 1101 5 0.057472428265683106 1101 6 0.01551968790904823 1101 7 -0.04126992242152028 1101 8 -0.018877308893230804 1101 9 0.20079428166368984 1101 10-11 -0.014893513206274633 1101 12-13 -0.017552854964293374 1101 14-15 -0.29593375266595245 1101 16-17 -0.1551484043701734 1101 18-19 0.04642056698438779 1101 20-21 -0.054153537036874866 1101 22-23 -0.10822444160912426 1101 24-25 -0.129416976642041 1101 26-27 -0.2224543960801313 1101 28-29 -0.15791602681347428 1101 30-31 -0.0726332639552183 1101 32-33 0.06423640987434709 1101 34-35 0.16716121179076282 1101 36-37 0.21960128776491672 1101 38-39 0.2143418804892505 1101 40-41 0.6454093276444368 1101 42-43 0.8373308664348045 1101 44-45 0.40732823580239597 1101 46-47 0.6859518936775046 1101 48-49 0.7192881971717995 1101 50-51 1.0630906414997483 1101 52-53 1.0991897470102288 1101 54-55 0.9664955627154868 1101 56-57 1.1661862882696816 1101 58-59 1.3688114013446828 1101 60-61 1.3369211953468572 1101 62-63 1.5011164869987965 1101 64-65 1.2564312903343549 1101 66-67 1.6312798825966333 1101 68-69 1.5223257509663455 1101 70-71 1.6478632570697407 1101 72-73 1.7899083552269666 1101 74-75 1.642565257479589 1101 76-77 1.7832303672982661 1101 78-79 1.4519338788831568 1101 80-81 1.6321840130957135 1101 82-83 1.492516578849461 1101 84-85 1.378137426836986 1101 86-87 1.2932131853935154 1101 88-89 1.3535715754445832 1101 90-91 1.6013973230955756 1101 92-93 1.8521891502319328 1101 94-95 1.8681743708314649 1101 96-97 1.6497163173850247 1101 98-99 2.0307822473088635 1101 100-101 2.0033016910896606 1102 1 -0.011511397645740828 1102 2 -0.030817414349936456 1102 3 -0.08043610574843996 1102 4 -0.11313435367677016 1102 5 -0.0970810682516614 1102 6 -0.10662946306276666 1102 7 -0.1185004593491783 1102 8 -0.11220619313564839 1102 9 -0.17541636518691917 1102 10-11 -0.06278885714461069 1102 12-13 -0.29077704953679984 1102 14-15 -0.1855786936054713 1102 16-17 -0.2986946018978074 1102 18-19 -0.12097727896744459 1102 20-21 0.05919445589099581 1102 22-23 -0.16232950817143887 1102 24-25 -0.23916708736919645 1102 26-27 -0.1763859000849166 1102 28-29 -0.09874151722372204 1102 30-31 -0.19713859266182254 1102 32-33 -0.13563479854595784 1102 34-35 -0.3526856546608883 1102 36-37 -0.19713189521154817 1102 38-39 -0.26700134486961247 1102 40-41 0.14828763340500473 1102 42-43 0.2239822733597876 1102 44-45 0.26484909991827976 1102 46-47 0.22730383317306035 1102 48-49 0.6305432369075419 1102 50-51 0.5487141163757911 1102 52-53 0.6787566568165175 1102 54-55 0.7238890756048448 1102 56-57 0.663290578241579 1102 58-59 0.8642106732461521 1102 60-61 1.1086552245308177 1102 62-63 1.13939999411474 1102 64-65 1.0534189116991115 1102 66-67 1.0332980574531128 1102 68-69 0.8243071968168714 1102 70-71 1.0499768553240365 1102 72-73 1.1359213976249656 1102 74-75 1.3688117331154146 1102 76-77 1.3809403257800845 1102 78-79 1.265464556605334 1102 80-81 1.2919833959043814 1102 82-83 1.4837820371398323 1102 84-85 1.4542912997666306 1102 86-87 1.133653261300129 1102 88-89 1.0167431851232784 1102 90-91 1.148339033202891 1102 92-93 0.944534393285533 1102 94-95 0.774209330603604 1102 96-97 1.1693381286531448 1102 98-99 1.5239885815020102 1102 100-101 1.5863308498393849 1103 1 0.1125658568662935 1103 2 0.1345184901164025 1103 3 0.07157543756745 1103 4 -0.0019110902795986817 1103 5 0.05280460337313997 1103 6 -0.17019804837048724 1103 7 0.04513139993485282 1103 8 -0.20267291403715149 1103 9 0.10104571562298759 1103 10-11 0.1540743271098819 1103 12-13 0.1441578853981369 1103 14-15 -6.872804531923293E-4 1103 16-17 -0.010853248839062246 1103 18-19 -0.031706570131518674 1103 20-21 -0.40301524544914 1103 22-23 -0.04352675893390767 1103 24-25 0.05404251018823203 1103 26-27 -0.032989158862086754 1103 28-29 -0.279877502707528 1103 30-31 -0.0416310784586571 1103 32-33 0.007372757505073935 1103 34-35 -0.009715594299521513 1103 36-37 0.09576133246428498 1103 38-39 0.0993290323303313 1103 40-41 0.39385678114806666 1103 42-43 0.1775939117540375 1103 44-45 0.33363611435746776 1103 46-47 0.44239852652862766 1103 48-49 0.338149672774847 1103 50-51 0.7031988686866271 1103 52-53 0.8221416806363209 1103 54-55 0.4607597255189759 1103 56-57 0.5166682033583321 1103 58-59 0.6245370531377468 1103 60-61 0.9700291402525565 1103 62-63 1.0463127597231612 1103 64-65 0.7853331001350412 1103 66-67 0.9957245307491469 1103 68-69 0.8400193973116785 1103 70-71 0.9813471888682805 1103 72-73 0.9515237893143116 1103 74-75 1.485212414733489 1103 76-77 1.4787195431245195 1103 78-79 1.1155194610080663 1103 80-81 1.3405009770306577 1103 82-83 1.3454371705340975 1103 84-85 1.493227393050553 1103 86-87 1.534281314102092 1103 88-89 1.7139587953823678 1103 90-91 1.8161623749392781 1103 92-93 1.5887873418863059 1103 94-95 1.8262061434699781 1103 96-97 1.7146300761190076 1103 98-99 2.228104068226191 1103 100-101 2.8255307520827664 1104 1 0.04931799361843048 1104 2 0.1977663533642655 1104 3 -0.050361884369870324 1104 4 0.014898026529522213 1104 5 0.017476968045507135 1104 6 0.17396149578905806 1104 7 0.14370689851035223 1104 8 -0.0172028285670649 1104 9 0.23694315152042833 1104 10-11 -0.024415701380149812 1104 12-13 -0.06467402343376705 1104 14-15 -0.04271007247598391 1104 16-17 -0.13222077020658674 1104 18-19 0.2957863073613609 1104 20-21 -0.12039416282805604 1104 22-23 -0.046660662067807834 1104 24-25 0.2993416554873747 1104 26-27 0.30960343373050137 1104 28-29 0.2563048334748075 1104 30-31 0.2576566708290926 1104 32-33 0.1717602218925407 1104 34-35 0.40908782450389225 1104 36-37 -0.021332684629733478 1104 38-39 0.01400139700270131 1104 40-41 0.42704766433895003 1104 42-43 0.19340587756600058 1104 44-45 0.19018882091017986 1104 46-47 0.01875180288190137 1104 48-49 -0.1067221220969472 1104 50-51 0.1739965894843536 1104 52-53 0.26160037009501025 1104 54-55 0.3829819477412002 1104 56-57 0.38376222045235053 1104 58-59 -0.056517077916385716 1104 60-61 0.3248724450958598 1104 62-63 0.36924723265763504 1104 64-65 0.2502903650923045 1104 66-67 0.5403114253360428 1104 68-69 0.4316148389071195 1104 70-71 0.365535223056316 1104 72-73 0.8539454417359664 1104 74-75 0.7387736682947406 1104 76-77 0.895671110076087 1104 78-79 0.5489952444838515 1104 80-81 0.5682787548084356 1104 82-83 0.31737449247141925 1104 84-85 0.537244487067646 1104 86-87 0.5243098041305849 1104 88-89 0.5999986814222495 1104 90-91 0.3551937139706176 1104 92-93 1.012149165248129 1104 94-95 0.9139554312192679 1104 96-97 1.063205574694507 1104 98-99 1.2526054927276178 1104 100-101 1.4256732022252159 1105 1 -0.06310121496330368 1105 2 -0.23402543147492594 1105 3 -0.00178477800495358 1105 4 -0.04700315230168428 1105 5 -0.014928349235660221 1105 6 -0.37931357125575715 1105 7 -0.043026909442417605 1105 8 -0.4001319636406748 1105 9 -0.1246010980599408 1105 10-11 -0.02559453258155031 1105 12-13 -0.042866610881439726 1105 14-15 -0.6006580186857775 1105 16-17 -0.24206622948770473 1105 18-19 -0.14778414275536278 1105 20-21 -0.08275645566438783 1105 22-23 -0.1150540945696008 1105 24-25 -0.32321241447075977 1105 26-27 -0.38643754560619215 1105 28-29 -0.5210716400641999 1105 30-31 -0.686899675758859 1105 32-33 -0.2943353579727912 1105 34-35 -0.3210736618652703 1105 36-37 -0.25110637219122367 1105 38-39 0.024761366904883175 1105 40-41 0.10451095850653047 1105 42-43 0.546586406593029 1105 44-45 0.5907039566397572 1105 46-47 0.5858197737873496 1105 48-49 0.5144389591092775 1105 50-51 0.22982225366215658 1105 52-53 0.34512181479027504 1105 54-55 0.605576533992668 1105 56-57 0.739464406402842 1105 58-59 1.1355552340204866 1105 60-61 0.6416558372742891 1105 62-63 0.8685700317547003 1105 64-65 0.6729582665412437 1105 66-67 1.00442749486972 1105 68-69 0.8491564645751559 1105 70-71 0.9010815177448244 1105 72-73 0.7705976382978683 1105 74-75 1.3986876194050808 1105 76-77 1.2344336267167044 1105 78-79 0.6044562620216176 1105 80-81 0.6493653632088119 1105 82-83 0.6927116459124107 1105 84-85 0.9345800584438493 1105 86-87 0.9518435194746822 1105 88-89 0.9902188199493906 1105 90-91 1.3993622617509018 1105 92-93 1.3151898165957796 1105 94-95 1.3884050849014287 1105 96-97 1.3984856194553643 1105 98-99 1.5884354014694892 1105 100-101 1.828368500405844 1106 1 -0.29673201037549646 1106 2 -0.011020290936397004 1106 3 -0.04866845837738154 1106 4 -0.12238130888065513 1106 5 -0.2138833642494049 1106 6 -0.36323796103656747 1106 7 -0.4323295261241995 1106 8 -0.17858952079487977 1106 9 -0.23120925681890014 1106 10-11 -0.6124738529896945 1106 12-13 -0.6010826822723345 1106 14-15 -0.5433491011523941 1106 16-17 -0.40653181741483024 1106 18-19 -0.36615154709986086 1106 20-21 0.03981831420684756 1106 22-23 -0.30871034654740015 1106 24-25 -0.32897530273612574 1106 26-27 -0.21412050588129006 1106 28-29 -0.5646712828284066 1106 30-31 -0.6913967679439708 1106 32-33 -0.6707831122395831 1106 34-35 -0.35618895479157686 1106 36-37 -0.5188644363670889 1106 38-39 -0.5897369207543051 1106 40-41 -0.30416888650376706 1106 42-43 -0.5638191404066788 1106 44-45 -0.6002976396697477 1106 46-47 -1.0543530776996093 1106 48-49 -1.5236404063049491 1106 50-51 -0.9238946158834906 1106 52-53 -0.7679946454813162 1106 54-55 -1.1299424072392341 1106 56-57 -1.1257353432508204 1106 58-59 -0.39506648282092627 1106 60-61 -0.784217737826097 1106 62-63 -0.8351141380588771 1106 64-65 -0.5029487115112588 1106 66-67 -0.17568825514961972 1106 68-69 0.019792808393500394 1106 70-71 0.1510692113940415 1106 72-73 0.047690948565584534 1106 74-75 0.5110797630307431 1106 76-77 0.3232292217463453 1106 78-79 0.4633973532784843 1106 80-81 -0.07868186504937924 1106 82-83 0.044381441908274866 1106 84-85 -0.004675794104972653 1106 86-87 0.22704165172224222 1106 88-89 0.511652966908315 1106 90-91 0.5773120940329228 1106 92-93 0.7447236520282239 1106 94-95 0.01706270815832056 1106 96-97 0.47658523760688 1106 98-99 0.7834066749306672 1106 100-101 1.1347474094302648 1107 1 -0.41124366694322845 1107 2 -0.4247000691021583 1107 3 -0.32240095640894495 1107 4 -0.21782480619331324 1107 5 -0.10389055332201735 1107 6 -0.12664900482143793 1107 7 -0.3940220392185836 1107 8 -0.347361558725801 1107 9 -0.5821044675271949 1107 10-11 -0.396698973663419 1107 12-13 -0.6553944141541592 1107 14-15 -0.6355061652720764 1107 16-17 -0.023063261049074413 1107 18-19 -0.5464603348852819 1107 20-21 -0.6543379967718934 1107 22-23 -0.23664845205560425 1107 24-25 -0.3110368548911353 1107 26-27 -0.43764931352224323 1107 28-29 -0.3600565828866067 1107 30-31 -0.575310362137941 1107 32-33 -0.5453337952014792 1107 34-35 -0.45721254179647275 1107 36-37 -0.43598469928173955 1107 38-39 -0.617500434499135 1107 40-41 -0.3789352416439513 1107 42-43 -0.11758313342301108 1107 44-45 -0.47537894465759223 1107 46-47 -0.09565601152591086 1107 48-49 -0.4631323785072041 1107 50-51 -0.48900707351931416 1107 52-53 0.03632564482028755 1107 54-55 -0.21506445030519927 1107 56-57 -0.17838674169660962 1107 58-59 -0.08374540514471107 1107 60-61 0.16162458184799888 1107 62-63 -0.3626208992104978 1107 64-65 -0.3337145189125792 1107 66-67 0.03469481971943722 1107 68-69 -0.09170628441400552 1107 70-71 0.03903949656058714 1107 72-73 -0.048984961194438625 1107 74-75 -0.27160484208377156 1107 76-77 0.2498835642885382 1107 78-79 0.07939817488678003 1107 80-81 0.2113800979097782 1107 82-83 -0.2859222108252837 1107 84-85 0.279857429680586 1107 86-87 0.49683727665805577 1107 88-89 0.19767877910235043 1107 90-91 0.3310178897947935 1107 92-93 0.35048860358756784 1107 94-95 0.15363797090180498 1107 96-97 0.2055743170632489 1107 98-99 0.9169522570743815 1107 100-101 0.5855022620542769 1108 1 -0.009396239008843565 1108 2 0.08598634593208487 1108 3 -0.13166994828663192 1108 4 0.01683039367927819 1108 5 0.15709049461555225 1108 6 -0.024960837915884326 1108 7 0.25106475804212636 1108 8 -0.36543768549757516 1108 9 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#Quality Count 2 1.0 3 4.0 4 12.0 5 23.0 6 47.0 7 90.0 8 255.0 9 496.0 10 851.0 11 1227.0 12 1916.0 13 2555.0 14 3409.0 15 4778.0 16 6517.0 17 8413.0 18 7119.0 19 4495.0 20 4087.0 21 4134.0 22 4470.0 23 4876.0 24 5468.0 25 6001.0 26 7204.0 27 8645.0 28 10234.0 29 11970.0 30 14281.0 31 16918.0 32 20263.0 33 24449.0 34 30864.0 35 39693.0 36 45684.0 37 43774.0 38 17658.0 39 592.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.01988913171527 23.053061539054372 17.397622992783102 18.529426336447262 2 16.134073232399658 24.498105774018978 38.91650824132742 20.45131275225395 3 18.614312479881587 29.08579179196254 32.655245368982015 19.64465035917386 4 12.74289974771166 20.788614284967522 38.01988043128375 28.448605536037064 5 11.553487809038534 40.37131396059054 34.08131709705997 13.993881133310957 6 27.18936482214635 40.612920354469246 17.297570933741984 14.900143889642422 7 24.498380897618254 37.44157062560355 21.42359955209878 16.63644892467941 8 26.32134986642749 35.432618103683076 19.75359930448754 18.492432725401887 9 26.97834502150091 16.68019357696445 21.17791417794444 35.1635472235902 10-11 23.921584271734076 29.400120504136485 26.811620120339064 19.866675103790378 12-13 25.699652082757257 27.680400386849858 28.330423705146675 18.289523825246217 14-15 22.55243167993221 27.871258662954336 28.582315605285675 20.993994051827784 16-17 18.79781992059933 31.75050141275968 29.008894745964625 20.442783920676362 18-19 18.811851224162453 28.023952260553052 34.71550844216764 18.44868807311685 20-21 20.19104582733793 26.749304625102827 35.32064279877735 17.73900674878189 22-23 19.776103490920196 27.299389360651382 35.12297387828207 17.80153327014635 24-25 19.377501062672213 27.727147044945877 35.13462578118444 17.76072611119747 26-27 19.092254699124002 28.02375423691509 35.05813377646697 17.82585728749395 28-29 18.755631614352552 27.957844228343305 35.29122066573764 17.99530349156651 30-31 18.826347008848 27.69580600431395 35.378653651450456 18.099193335387596 32-33 18.69781249226215 28.163852610785394 34.92405213041959 18.214282766532865 34-35 19.067578609690404 28.19879330789357 34.764205319239665 17.969422763176357 36-37 19.29510582629246 27.88625289911493 34.54685767581086 18.271783598781752 38-39 18.877408545099133 28.47142073037363 34.494864109015424 18.15630661551181 40-41 18.90528169256271 28.381514419935485 34.5147156937595 18.198488193742303 42-43 19.10240976287603 28.57168408235707 33.74245874235556 18.583447412411346 44-45 19.10870958778231 28.86638072153915 33.32118204103193 18.703727649646602 46-47 19.4971841099614 29.233533164774272 32.47737796204945 18.791904763214877 48-49 19.565277200782454 29.49036104469933 31.80717687421074 19.13718488030748 50-51 19.684955966467935 29.79437262190039 31.106161943253007 19.414509468378668 52-53 19.90395435149241 30.210497065806813 30.681371105969358 19.20417747673142 54-55 19.621539976834594 30.206370211817656 30.604886745370358 19.567203065977388 56-57 19.85622040701785 30.27817747122895 30.410511922481177 19.455090199272025 58-59 19.721409843372133 29.643604889496604 30.855111658912765 19.779873608218494 60-61 19.78743950719861 29.87099454429903 30.94782831186911 19.393737636633258 62-63 19.68646914626396 29.655710327864792 31.283479102987016 19.374341422884232 64-65 19.544092683638127 29.46670041516151 31.238909079904147 19.75029782129622 66-67 19.523458413692353 29.877459948882034 30.69829120732489 19.900790430100724 68-69 19.397864490622414 29.581014270661093 30.677656937379115 20.343464301337374 70-71 19.531574559871025 29.729030767072107 30.591405689005786 20.147988984051086 72-73 19.680416427079866 29.680471451799722 30.307478134551946 20.331633986568466 74-75 19.31986695022739 29.93427297213273 29.865492072313486 20.880368005326392 76-77 19.02507201360211 29.64222927150022 30.5971832845906 20.735515430307068 78-79 19.073356205275218 29.51044506744655 30.32921289889483 21.0869858283834 80-81 18.78516423503259 29.93716176992514 29.96880098384199 21.308873011200284 82-83 18.846516797671352 29.59917242821338 30.00800609673896 21.54630467737631 84-85 18.524535590086717 29.482876260069553 29.93738169652683 22.0552064533169 86-87 18.460655949685396 29.424468942672494 29.885163409661793 22.229711697980317 88-89 18.447862702319018 29.008069375166794 29.931109050741046 22.612958871773145 90-91 18.468114612124058 29.246535483010007 29.70562783665282 22.57972206821312 92-93 18.388080151653135 29.220329003662016 29.54484800294942 22.846742841735427 94-95 18.00752738167622 29.0002283525874 29.34770945847422 23.64453480726216 96-97 18.357484599956532 29.073686353594354 28.959234936295132 23.609594110153985 98-99 18.42942942116746 29.340005997694462 28.58974394246615 23.640820638671922 100-101 18.53209822699963 28.786250751533675 28.57245059826896 24.10920042319773 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 914.0 1 586.5 2 301.0 3 421.0 4 435.5 5 441.5 6 720.5 7 1382.5 8 2056.0 9 2255.0 10 1943.5 11 1623.5 12 1595.0 13 1597.5 14 1608.5 15 1619.0 16 1593.0 17 1648.5 18 1695.5 19 1740.5 20 1850.0 21 1939.0 22 2159.0 23 2447.0 24 2667.5 25 3051.0 26 3694.0 27 4367.0 28 4840.0 29 5388.0 30 6216.0 31 6993.5 32 7827.5 33 8934.0 34 10178.0 35 11008.0 36 12012.0 37 12796.5 38 12882.5 39 12696.0 40 12347.0 41 12463.5 42 12534.0 43 12720.5 44 12907.5 45 12712.5 46 12036.5 47 11378.5 48 11215.0 49 10755.0 50 9968.0 51 8991.5 52 8164.0 53 7755.5 54 7363.0 55 6652.0 56 5840.0 57 5077.5 58 4517.0 59 4074.5 60 3393.0 61 2680.5 62 2146.0 63 1650.0 64 1175.5 65 857.5 66 626.0 67 484.0 68 368.5 69 236.0 70 163.5 71 128.0 72 99.0 73 86.0 74 67.5 75 47.5 76 40.0 77 29.0 78 17.5 79 12.0 80 13.0 81 8.0 82 1.5 83 0.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04374465228503906 2 0.0 3 0.0 4 0.0 5 0.001925865194938826 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006740528182285892 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.1268539891546277E-4 24-25 6.878089981924379E-4 26-27 2.751235992769752E-4 28-29 0.0031639213916852147 30-31 2.751235992769752E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.1268539891546277E-4 40-41 1.375617996384876E-4 42-43 0.015269359759872123 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.751235992769752E-4 86-87 0.0 88-89 0.0 90-91 0.010592258572163544 92-93 0.003301483191323702 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.013618618164210273 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 363473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.910124224053895 #Duplication Level Percentage of deduplicated Percentage of total 1 82.02107170560558 48.31871523167256 2 10.019155937082209 11.804594417473599 3 3.0982312858188488 5.475515697673159 4 1.356100230361346 3.1955213212342 5 0.7824305660563025 2.304654092153679 6 0.5227279094422841 1.847637964837497 7 0.36744067971920524 1.5152183261060426 8 0.2759496190598882 1.300498107071869 9 0.20281332455552117 1.0752982329473202 >10 1.259458831733715 13.675388679812567 >50 0.06792023283142709 2.6606529479814642 >100 0.023420769941864025 2.2616377890116848 >500 0.0014052461965118415 0.6622658240151347 >1k 0.0014052461965118415 2.468319914923075 >5k 4.684153988372805E-4 1.4340814530861061 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5197 1.42981734544244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4275 1.176153386909069 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3207 0.8823213828812595 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1463 0.40250582574221466 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 962 0.26466890250445013 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 874 0.2404580257680763 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 564 0.15516970999221402 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 431 0.11857827128837631 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 404 0.11114993410789797 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.3756179963848758E-4 0.0 12-13 0.0 0.0 0.0 2.7512359927697516E-4 0.0 14-15 0.0 0.0 0.0 2.7512359927697516E-4 0.0 16-17 0.0 0.0 0.0 5.502471985539503E-4 0.0 18-19 0.0 0.0 0.0 8.253707978309255E-4 0.0 20-21 0.0 0.0 0.0 8.253707978309255E-4 0.0 22-23 0.0 0.0 0.0 0.0015131797960233635 0.0 24-25 0.0 0.0 0.0 0.002888797792408239 0.0 26-27 0.0 0.0 0.0 0.004677101187708578 0.0 28-29 0.0 0.0 0.0 0.009766887774332619 0.0 30-31 0.0 0.0 0.0 0.020771831745411626 0.0 32-33 0.0 0.0 0.0 0.041130978091907786 0.0 34-35 0.0 0.0 0.0 0.06506673122900464 0.0 36-37 0.0 0.0 0.0 0.09739375414404922 0.0 38-39 0.0 0.0 0.0 0.16328585617088476 0.0 40-41 0.0 0.0 0.0 0.24994978994313197 0.0 42-43 0.0 0.0 0.0 0.3434918136973035 0.0 44-45 0.0 0.0 0.0 0.45753054559760975 0.0 46-47 0.0 0.0 0.0 0.5999070082234443 0.0 48-49 0.0 0.0 0.0 0.7578279542084281 0.0 50-51 0.0 0.0 0.0 0.9505520355019492 0.0 52-53 0.0 0.0 0.0 1.1573074203585962 0.0 54-55 0.0 0.0 0.0 1.396527389929926 0.0 56-57 0.0 0.0 0.0 1.6983379783367678 0.0 58-59 0.0 0.0 0.0 1.9982227015486709 0.0 60-61 0.0 0.0 0.0 2.3503809086231993 0.0 62-63 0.0 0.0 0.0 2.7436700937896346 0.0 64-65 0.0 0.0 0.0 3.1547047511094357 0.0 66-67 0.0 0.0 0.0 3.6026059707323514 0.0 68-69 0.0 0.0 0.0 4.16399567505702 0.0 70-71 0.0 0.0 0.0 4.8896341681500415 0.0 72-73 0.0 0.0 0.0 5.585146627122235 0.0 74-75 0.0 0.0 0.0 6.263601422939255 0.0 76-77 0.0 0.0 0.0 7.110569423313423 0.0 78-79 0.0 0.0 0.0 7.982986356620712 0.0 80-81 0.0 0.0 0.0 8.905613346796049 0.0 82-83 0.0 0.0 0.0 9.79687624665381 0.0 84-85 0.0 0.0 0.0 10.72087885482553 0.0 86-87 0.0 0.0 0.0 11.58779331614728 0.0 88-89 0.0 0.0 0.0 12.622395611228345 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1405 0.0 65.640335 1 GTATCAA 2900 0.0 48.522045 1 CGGGTCG 40 5.380059E-4 47.505775 5 TATCAAC 4045 0.0 34.998077 2 GTCGGGA 55 0.0025757772 34.549656 8 ATCAACG 4060 0.0 34.517746 3 GGCCCGC 70 2.0491141E-4 33.9327 9 TCAACGC 4135 0.0 33.89167 4 GTGGTAT 515 0.0 33.230785 1 CAACGCA 4230 0.0 33.13051 5 TGGTATC 520 0.0 32.88862 2 AACGCAG 4330 0.0 32.356464 6 CTAGACA 75 3.0721427E-4 31.67052 4 GGTCGGG 60 0.0039450773 31.670519 7 TGCGGGA 30 0.003952974 31.661808 46-47 ACGAATC 40 4.496074E-4 29.682941 72-73 TCTAGAC 65 0.0058354135 29.234325 3 ACGCAGA 5085 0.0 27.279623 7 CGCAGAG 5075 0.0 27.239767 8 CACTAGG 70 0.008351086 27.164852 1 >>END_MODULE