FastQCFastQC Report
Thu 26 May 2016
SRR937167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369331
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.6419715648023047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.5268986356411999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.35225854314964083No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6290.17030793515843512No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.1231957241607122No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA3710.10045189816181149No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG250.005009290857.026665
GGTATCA9400.046.5300751
GTATCAA17950.042.6417661
TATCAAC23050.033.3995632
ATCAACG23200.033.1836173
TCAACGC23200.033.1836174
CAACGCA23450.033.03255
AACGCAG24200.032.0044176
CGCAGAG26750.028.9535278
ACGCAGA27200.028.299837
GCAGAGT28650.026.0383059
GTACTGA1351.3360654E-524.63786
TACATGG15350.023.2193242
GTACATG16100.023.0325761
GCCTTAT1452.3130597E-522.9511171
CCCTATA1452.3203651E-522.9417612
GTGTAGC2303.5706762E-922.7372881
TAAGACT1903.2596472E-722.5105254
AGTACTT18550.022.4009212-13
GAGTACT17700.022.26929912-13
Produced by FastQC (version 0.11.5)