##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937167_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 369331 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91673864365569 33.0 31.0 34.0 30.0 34.0 2 32.04698224627773 33.0 31.0 34.0 30.0 34.0 3 32.07290479271981 34.0 31.0 34.0 30.0 34.0 4 35.67697810365228 37.0 35.0 37.0 35.0 37.0 5 35.680007906187136 37.0 35.0 37.0 35.0 37.0 6 35.73964817467258 37.0 35.0 37.0 35.0 37.0 7 35.68072812734376 37.0 35.0 37.0 35.0 37.0 8 35.67067752233092 37.0 35.0 37.0 35.0 37.0 9 37.32132964738947 39.0 37.0 39.0 35.0 39.0 10-11 37.22977491735056 39.0 37.0 39.0 35.0 39.0 12-13 37.10666448253735 39.0 37.0 39.0 34.0 39.0 14-15 38.26961181162697 40.0 38.0 41.0 34.0 41.0 16-17 38.19986813996117 40.0 38.0 41.0 34.0 41.0 18-19 38.15940443667063 40.0 38.0 41.0 33.5 41.0 20-21 38.051605470431674 40.0 38.0 41.0 34.0 41.0 22-23 38.098117948398595 40.0 38.0 41.0 34.0 41.0 24-25 38.05731850291473 40.0 38.0 41.0 34.0 41.0 26-27 37.97435633618624 40.0 38.0 41.0 34.0 41.0 28-29 37.829061194429926 40.0 38.0 41.0 33.0 41.0 30-31 37.54850527034016 40.0 37.0 41.0 33.0 41.0 32-33 37.276545970958296 40.0 36.5 41.0 32.5 41.0 34-35 37.28094852584809 40.0 37.0 41.0 33.0 41.0 36-37 37.12584917052725 40.0 37.0 41.0 32.0 41.0 38-39 36.97366860620961 40.0 36.0 41.0 31.0 41.0 40-41 36.75358418329358 39.5 36.0 41.0 31.0 41.0 42-43 36.477958795768565 39.0 36.0 41.0 30.0 41.0 44-45 36.20107979021528 39.0 35.5 41.0 30.0 41.0 46-47 35.76189921777484 39.0 35.0 41.0 28.5 41.0 48-49 35.385043497567224 39.0 35.0 40.0 26.5 41.0 50-51 34.39648445432417 38.0 34.0 39.5 25.0 40.5 52-53 34.469111718214826 38.0 34.5 39.5 24.5 40.5 54-55 34.76407071163807 39.0 35.0 40.0 24.5 41.0 56-57 34.50825411351877 38.0 34.0 40.0 23.5 41.0 58-59 34.24066758544504 38.0 34.0 40.0 22.5 41.0 60-61 33.9289472045401 38.0 34.0 40.0 21.5 41.0 62-63 33.55680676682975 37.5 33.5 40.0 19.5 41.0 64-65 33.149272603707786 37.0 33.0 40.0 18.0 41.0 66-67 32.7068672816525 36.0 32.5 39.0 14.0 41.0 68-69 32.222848880814226 36.0 32.0 39.0 9.0 40.0 70-71 31.755415873565987 35.0 31.0 38.5 7.0 40.0 72-73 31.278045980434896 35.0 31.0 38.0 2.0 40.0 74-75 30.740483468758377 35.0 30.5 37.0 2.0 39.0 76-77 30.236418009861076 34.5 30.0 37.0 2.0 39.0 78-79 29.710338422715665 34.0 29.0 36.0 2.0 39.0 80-81 29.16179118460135 34.0 29.0 36.0 2.0 37.5 82-83 28.607265569367314 34.0 28.5 35.0 2.0 37.0 84-85 28.087385028605777 34.0 27.0 35.0 2.0 36.5 86-87 27.51624694379838 33.5 26.0 35.0 2.0 36.0 88-89 26.969412261629813 33.0 25.5 35.0 2.0 36.0 90-91 26.415383490689923 33.0 24.5 35.0 2.0 35.0 92-93 25.780308178842283 32.0 22.5 35.0 2.0 35.0 94-95 25.012509104299397 32.0 19.0 34.0 2.0 35.0 96-97 24.18999082124165 31.5 6.0 34.0 2.0 35.0 98-99 23.037988958413997 31.0 2.0 34.0 2.0 35.0 100-101 19.965950597160813 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2047454005191831 1101 2 0.3536140297043744 1101 3 0.10962469902705152 1101 4 0.17877329151991006 1101 5 0.12748373977724725 1101 6 0.10781582753968877 1101 7 0.18696200640737715 1101 8 0.11183544771840559 1101 9 0.12024715600778535 1101 10-11 0.32625335797267496 1101 12-13 0.2690969870160842 1101 14-15 0.38141798167930574 1101 16-17 0.4055146670976697 1101 18-19 0.23483436439467198 1101 20-21 0.33975351131643095 1101 22-23 0.4187188097905974 1101 24-25 0.39482650662213814 1101 26-27 0.40697982179946735 1101 28-29 0.44976775014934844 1101 30-31 0.47277017100763175 1101 32-33 0.412428755083063 1101 34-35 0.7441003158548298 1101 36-37 0.8691455066805318 1101 38-39 0.8308708621014134 1101 40-41 0.6327852278278172 1101 42-43 0.7244080861841411 1101 44-45 0.8217356399908482 1101 46-47 1.0914427489502145 1101 48-49 1.1894703356715084 1101 50-51 1.3941114005861905 1101 52-53 1.3876846454583855 1101 54-55 1.294684193421233 1101 56-57 1.4330337687189214 1101 58-59 1.416451644696643 1101 60-61 1.5460033296970934 1101 62-63 1.6826529439238485 1101 64-65 1.7015253531777645 1101 66-67 1.7860540390918516 1101 68-69 1.6713755230235705 1101 70-71 1.7038613384748764 1101 72-73 1.7948295376091643 1101 74-75 1.68095444323572 1101 76-77 1.7184050335176089 1101 78-79 1.59234416588912 1101 80-81 1.527734347414416 1101 82-83 1.5457821329397206 1101 84-85 1.3737685452304866 1101 86-87 1.20016536585036 1101 88-89 1.0600581984717188 1101 90-91 1.028788573989182 1101 92-93 1.048293963479118 1101 94-95 0.9448745198803472 1101 96-97 1.3529321496998428 1101 98-99 1.589901605018273 1101 100-101 1.7952625407351626 1102 1 0.13024347518852508 1102 2 0.032609323340551555 1102 3 -0.02444156211103632 1102 4 0.13632300597570435 1102 5 0.07855522814155336 1102 6 0.06437497332535713 1102 7 0.11071612215044269 1102 8 0.19102513464289927 1102 9 0.18908935330301802 1102 10-11 0.08197354325087503 1102 12-13 0.2349595723538087 1102 14-15 0.2233711226655899 1102 16-17 0.48405792658014946 1102 18-19 0.17149610147077254 1102 20-21 0.1259115930546244 1102 22-23 0.15868058221093406 1102 24-25 0.10847077620563539 1102 26-27 0.3426254121411887 1102 28-29 0.38572957837637745 1102 30-31 0.5099537005944796 1102 32-33 0.6055663929720723 1102 34-35 0.5806425336125187 1102 36-37 0.8632206968883125 1102 38-39 0.7393269701431606 1102 40-41 0.794008417365923 1102 42-43 0.9311509289767059 1102 44-45 1.0344846773255227 1102 46-47 1.0539778606565946 1102 48-49 1.1217256698605382 1102 50-51 0.8044533095468793 1102 52-53 0.9069426025616494 1102 54-55 1.2006010972663148 1102 56-57 1.1754905650431269 1102 58-59 1.3059939368632456 1102 60-61 1.406672637880213 1102 62-63 1.45487563120178 1102 64-65 1.1749567202555369 1102 66-67 1.599243484187376 1102 68-69 1.5629059283661348 1102 70-71 1.4296551885781206 1102 72-73 1.4089146800092287 1102 74-75 1.5526688246186104 1102 76-77 1.4135726395968184 1102 78-79 1.2744855614283033 1102 80-81 1.5396095450630973 1102 82-83 1.5385970221634508 1102 84-85 1.7596880533875634 1102 86-87 1.8386519815469207 1102 88-89 1.4934250158164346 1102 90-91 1.6967620472112763 1102 92-93 1.8194865523512647 1102 94-95 1.7016666585785636 1102 96-97 1.511583581141334 1102 98-99 1.9555609610091231 1102 100-101 2.237983953760441 1103 1 0.11176601768667993 1103 2 -0.06879085732792234 1103 3 0.12107868097018581 1103 4 0.2544144899208476 1103 5 0.10017385341871687 1103 6 0.06778303770902738 1103 7 -0.10005007642063646 1103 8 0.010130825699810941 1103 9 0.06457182024890074 1103 10-11 0.003290370055509584 1103 12-13 -0.06621271967214426 1103 14-15 0.10830788869991892 1103 16-17 0.04950918715336172 1103 18-19 0.09331592572961966 1103 20-21 -0.17239669305853766 1103 22-23 -0.3593041430908599 1103 24-25 -0.26402450177744896 1103 26-27 -0.18503944784074378 1103 28-29 -0.16397478230359752 1103 30-31 0.04220712918937153 1103 32-33 -0.13883904350652898 1103 34-35 -0.17745675192578858 1103 36-37 0.009428711998772599 1103 38-39 0.06893401621362472 1103 40-41 0.2597840599708334 1103 42-43 0.24360473533295135 1103 44-45 0.0791077371924871 1103 46-47 0.12752840676811417 1103 48-49 0.20418645658961765 1103 50-51 0.40174738854828007 1103 52-53 0.4752065427768102 1103 54-55 0.6252438664212718 1103 56-57 0.377100156896617 1103 58-59 0.32470749521095144 1103 60-61 0.3880001405491811 1103 62-63 0.4632418133484535 1103 64-65 0.25291106040499756 1103 66-67 0.37998792041961593 1103 68-69 0.499487188118124 1103 70-71 0.5105248609112039 1103 72-73 0.47772267869527596 1103 74-75 0.24843748685068334 1103 76-77 0.07576365544636943 1103 78-79 0.19713687127473634 1103 80-81 0.10435250309873467 1103 82-83 0.1814893601777392 1103 84-85 0.3778616593660473 1103 86-87 0.596484370476869 1103 88-89 0.3813592360677269 1103 90-91 0.6308693807088517 1103 92-93 0.5267728297594942 1103 94-95 0.2095401085251858 1103 96-97 0.625312332065203 1103 98-99 0.7083941711594051 1103 100-101 1.2277289648469178 1104 1 0.07783973857426219 1104 2 0.19899751604453542 1104 3 -0.06854171147434229 1104 4 0.09221019870500413 1104 5 0.15089516890255084 1104 6 0.08774147037606639 1104 7 -0.06798821444863279 1104 8 -0.17864905082075921 1104 9 0.1797225018309021 1104 10-11 0.018595400925363492 1104 12-13 0.031768513899592676 1104 14-15 -0.028856669035889126 1104 16-17 0.18593256266531455 1104 18-19 0.1175227843395561 1104 20-21 0.18610627167035432 1104 22-23 0.14326325100413584 1104 24-25 0.25011908890657963 1104 26-27 0.06282869813394854 1104 28-29 -0.03418384153705034 1104 30-31 0.227701352552657 1104 32-33 0.27160016426901024 1104 34-35 0.48975242956451837 1104 36-37 0.627796277253367 1104 38-39 0.41810725418660155 1104 40-41 0.4546309332515932 1104 42-43 0.03647104745472518 1104 44-45 -0.25487508571845297 1104 46-47 0.18759473111557412 1104 48-49 0.19297703058558824 1104 50-51 0.26718843017673777 1104 52-53 0.24131388430134848 1104 54-55 0.35367317139101573 1104 56-57 0.24581156957465566 1104 58-59 0.37377070200842866 1104 60-61 0.3908502536367777 1104 62-63 0.6694967800334553 1104 64-65 0.762416531399559 1104 66-67 0.47737315179072937 1104 68-69 0.702509989022829 1104 70-71 0.839334690362886 1104 72-73 0.9462491778700404 1104 74-75 1.140843807530434 1104 76-77 0.7135777027597747 1104 78-79 0.8366548735976203 1104 80-81 0.8401019376607515 1104 82-83 0.826058099098212 1104 84-85 0.661589271078256 1104 86-87 0.842151892941569 1104 88-89 0.3496565036268713 1104 90-91 0.23290037282328413 1104 92-93 0.4116297892313412 1104 94-95 0.49083022405791965 1104 96-97 0.37761228927530155 1104 98-99 0.4013154988689216 1104 100-101 0.15727080725782372 1105 1 0.4602583210535194 1105 2 0.17792899459682587 1105 3 0.32189403206317735 1105 4 0.25074775296529594 1105 5 0.2223417970001691 1105 6 0.1823656388664574 1105 7 0.1643650028886583 1105 8 0.18189929410495154 1105 9 0.27727185070742166 1105 10-11 0.5009034765900253 1105 12-13 0.5206672737675646 1105 14-15 0.4034597126965451 1105 16-17 0.2664616952951633 1105 18-19 0.4655072989401816 1105 20-21 0.4155054179510529 1105 22-23 0.45015439818042324 1105 24-25 0.36204154634700814 1105 26-27 0.6692077581759861 1105 28-29 0.45175001327289266 1105 30-31 0.48992391183270456 1105 32-33 0.30205093095516844 1105 34-35 0.43798395939775503 1105 36-37 0.6950645921327876 1105 38-39 0.7486129131466797 1105 40-41 0.7804021335867262 1105 42-43 0.9031839391941574 1105 44-45 0.6764373452147936 1105 46-47 0.7345637386488093 1105 48-49 0.7073465278156945 1105 50-51 0.21646002414725984 1105 52-53 0.24351679792546577 1105 54-55 0.6384376763132593 1105 56-57 0.8030801011340998 1105 58-59 0.6686866662706734 1105 60-61 0.9537524659398642 1105 62-63 0.7441249932177172 1105 64-65 1.0547901166595395 1105 66-67 1.2870630826697962 1105 68-69 1.4839123421679403 1105 70-71 1.255165333252524 1105 72-73 1.3948960110914292 1105 74-75 1.5080875653399417 1105 76-77 1.4911592647338736 1105 78-79 1.43141266143898 1105 80-81 1.600518830035778 1105 82-83 1.3415254418065672 1105 84-85 1.5072986453785617 1105 86-87 1.4562375393342073 1105 88-89 1.4370543931633897 1105 90-91 1.7868609226127425 1105 92-93 1.7272748095631556 1105 94-95 2.025134283919389 1105 96-97 1.7539204949479874 1105 98-99 1.1179558027987788 1105 100-101 0.981521636058929 1106 1 0.08607842038517433 1106 2 -0.7751132928927014 1106 3 -0.3767590633647089 1106 4 0.2846860692323858 1106 5 -0.14775038114277805 1106 6 0.07046423473376962 1106 7 0.08062800695594063 1106 8 0.36833009918190385 1106 9 0.3877425519562152 1106 10-11 -0.006893854127163479 1106 12-13 0.2802887506617111 1106 14-15 -0.11606938166852387 1106 16-17 -0.12347126605541803 1106 18-19 0.11774941474536149 1106 20-21 0.1855863548570511 1106 22-23 -0.12409397184021742 1106 24-25 -0.28220907192102374 1106 26-27 -0.08014842552626078 1106 28-29 0.08334402067166735 1106 30-31 -0.043201969582469246 1106 32-33 -0.1671429701628213 1106 34-35 -0.03433704945216931 1106 36-37 0.3153662660551646 1106 38-39 0.15894871955214285 1106 40-41 0.4079850632574633 1106 42-43 0.25964867236807265 1106 44-45 -0.03724324014707747 1106 46-47 0.22986796739775883 1106 48-49 -0.03186699437042506 1106 50-51 -0.5572129989279375 1106 52-53 -0.12404099225173582 1106 54-55 -0.14471375336768943 1106 56-57 0.25631655544358267 1106 58-59 -0.16432986856693788 1106 60-61 -0.22489122772621073 1106 62-63 -0.3734430162658029 1106 64-65 -0.11073709735317294 1106 66-67 0.26342071751354723 1106 68-69 0.3424209366045474 1106 70-71 -0.15921220879991793 1106 72-73 -0.3466372936278539 1106 74-75 -0.7892073842788747 1106 76-77 -0.6313804372711544 1106 78-79 -0.5876105772635789 1106 80-81 -0.5014034214817862 1106 82-83 -0.6953918022509065 1106 84-85 -0.848915714790671 1106 86-87 -0.5800205594660461 1106 88-89 -0.13310625433185663 1106 90-91 0.05162271058257417 1106 92-93 0.11354414960035086 1106 94-95 -0.494425468364625 1106 96-97 -0.6612081661185023 1106 98-99 0.21254570203641876 1106 100-101 0.5403695114651477 1107 1 0.11577038608857038 1107 2 0.2936651552919045 1107 3 -0.13520089675096614 1107 4 -0.026285667826996928 1107 5 -0.04774508845726899 1107 6 -0.01527727050599026 1107 7 0.1266214440259148 1107 8 -0.2840992434665566 1107 9 0.19868782558805975 1107 10-11 0.22606369853505726 1107 12-13 -0.0579006216257838 1107 14-15 0.0941560867341309 1107 16-17 0.08670127549216033 1107 18-19 -0.019185591076592345 1107 20-21 0.14755840947756838 1107 22-23 -0.0305060603339129 1107 24-25 -0.11615106408785181 1107 26-27 0.003211371234613125 1107 28-29 0.03786662043878408 1107 30-31 0.0016932352444669618 1107 32-33 -0.17844285372373747 1107 34-35 0.1819353121441054 1107 36-37 -0.283633416383708 1107 38-39 -0.27331813765120216 1107 40-41 -0.2407923263900429 1107 42-43 -0.28950714429330304 1107 44-45 0.0011287297904658544 1107 46-47 0.26552743601214246 1107 48-49 -0.2975730386172799 1107 50-51 -0.5234588970966669 1107 52-53 -0.09887427265821458 1107 54-55 0.014410836449187059 1107 56-57 -0.3432917858287752 1107 58-59 -0.5157244911984691 1107 60-61 -0.3477873852365221 1107 62-63 -0.051092005362868065 1107 64-65 0.06540347637393751 1107 66-67 0.40562194541657703 1107 68-69 0.013123275971544501 1107 70-71 0.2142926075438929 1107 72-73 0.12087884906295088 1107 74-75 0.16364801955302966 1107 76-77 0.009934265809189213 1107 78-79 -0.009649319721500405 1107 80-81 0.1788125200869679 1107 82-83 -0.12087079028943748 1107 84-85 0.06661302449163742 1107 86-87 -0.13843857576751972 1107 88-89 -0.5923393442016582 1107 90-91 -0.2945189216816324 1107 92-93 -0.18292562916539623 1107 94-95 -0.10906503648148558 1107 96-97 0.4751181808699627 1107 98-99 0.23780504362633792 1107 100-101 1.1467921853298648 1108 1 0.03313337807842842 1108 2 -0.02223123150000106 1108 3 -0.12442466209413539 1108 4 0.009774379427078372 1108 5 0.16952281910914735 1108 6 -0.025650116699736714 1108 7 0.22334823478255572 1108 8 0.017923461541222707 1108 9 0.05312221733588274 1108 10-11 0.19000365128098196 1108 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349.0 4 307.0 5 345.0 6 349.0 7 396.0 8 446.0 9 543.0 10 648.0 11 979.0 12 1336.0 13 1706.0 14 2041.0 15 2602.0 16 3465.0 17 4527.0 18 4050.0 19 2683.0 20 2605.0 21 2631.0 22 2766.0 23 3063.0 24 3393.0 25 3821.0 26 4478.0 27 5331.0 28 6123.0 29 7464.0 30 9140.0 31 11652.0 32 14639.0 33 19660.0 34 26570.0 35 37954.0 36 53568.0 37 69225.0 38 50407.0 39 6058.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7071844217092 19.388919375607845 15.643218455754113 22.260677746928838 2 14.774378008813635 20.21624087343501 41.48626229659537 23.52311882115598 3 18.63046429541135 24.843334238392615 31.08498506652186 25.441216399674175 4 11.976127573033281 16.909454751813104 38.65394484252941 32.460472832624205 5 12.730624801448847 37.37974927571254 35.39953243748863 14.49009348534998 6 30.377596108957867 37.15543540804916 17.979513402599096 14.487455080393882 7 26.77695596118613 33.57046888783334 21.513740924204384 18.13883422677614 8 25.86614985396784 34.74153936353865 20.400801276831373 18.991509505662137 9 25.514361957710886 16.785885891592397 21.2308853350852 36.46886681561152 10-11 23.97642957786628 27.05263843754327 28.162473564042212 20.80845842054824 12-13 25.220249817787472 24.630519139441105 28.529256477875936 21.619974564895482 14-15 22.020145267409287 25.906564176058023 27.380409528152345 24.692881028380345 16-17 20.717817045813867 29.209646709812954 28.192646494036282 21.879889750336897 18-19 20.673545953500362 27.213888832353 31.49858547208597 20.613979742060664 20-21 21.326913891180684 26.077988623040515 31.880183681445136 20.714913804333666 22-23 20.947594378153063 26.106607759755327 31.957225549606367 20.988572312485243 24-25 20.709059526588035 26.346363651166705 32.015148965734355 20.929427856510905 26-27 20.336563642333964 26.664703695361325 31.719807104146934 21.278925558157773 28-29 20.439447094668413 26.90330942476945 31.781742181085313 20.875501299476817 30-31 20.53887501357368 26.663182755999564 31.60142252144641 21.196519708980347 32-33 20.166753305274188 26.81489785918942 31.470037745477942 21.54831109005846 34-35 20.973801070540592 26.839923780984332 31.250475011671714 20.935800136803362 36-37 21.008736338445587 26.345529106201198 31.40563044664157 21.24010410871164 38-39 20.481875401099266 26.839086775833504 31.326649815409542 21.352388007657687 40-41 20.741894475204834 26.80694489857664 31.433711548193564 21.01744907802496 42-43 20.494661767180318 26.804062159066856 31.121191725874827 21.580084347878007 44-45 20.607818845722242 27.166221370679395 31.023538495148333 21.20242128845003 46-47 20.997711931341655 27.423982108008783 30.300217407235507 21.278088553414054 48-49 20.7191959771714 27.561533398027944 30.03956324350735 21.679707381293305 50-51 21.010044794353195 27.705307452151484 29.9568345323741 21.327813221121218 52-53 21.18846561133889 27.662032637323847 29.835727280132506 21.31377447120476 54-55 20.88758175434406 27.68511870751115 30.015295645694756 21.41200389245003 56-57 20.85223757475768 28.014864703519986 29.65573476897027 21.47716295275206 58-59 20.907249667978473 27.505626391336374 29.867015034911436 21.720108905773717 60-61 20.442850883083512 27.69486646236161 30.08941398634982 21.77286866820506 62-63 20.64959027513974 27.516416128507082 30.345010039615783 21.488983556737395 64-65 20.614856175422034 27.529478476506753 30.068046036888113 21.7876193111831 66-67 20.319465585682384 27.669561772662277 29.97492897900709 22.03604366264825 68-69 20.720212286804248 27.154749048996358 30.21464735536876 21.910391308830633 70-71 20.724535243865542 27.357900549038334 29.76297878055696 22.154585426539157 72-73 20.576056873066694 27.292966842133826 29.82973354317035 22.30124274162913 74-75 20.49437122888617 27.523110050378758 29.68506832271169 22.297450398023383 76-77 20.63767337151883 27.139520903642005 29.785501724046686 22.437304000792476 78-79 20.636536097247212 27.179119748794317 29.845059069703062 22.339285084255405 80-81 20.62861011871244 27.136287960097206 29.753009195498997 22.48209272569136 82-83 20.64337743986737 27.18948715130943 29.77591275833391 22.391222650489297 84-85 20.634593340703912 27.04916809669477 29.769725909396133 22.546512653205184 86-87 20.605037000861433 27.0337586227947 29.684119135312592 22.677085241031275 88-89 20.550632731061825 26.92244550182282 29.529349033909824 22.997572733205526 90-91 20.332183273342984 26.89268081561549 29.88998226745882 22.885153643582708 92-93 20.477595739628914 26.776160917200908 29.76900376513687 22.97723957803331 94-95 20.254521280204052 26.75444666042574 29.78604474473252 23.204987314637687 96-97 20.43784923592133 26.694718616598323 29.442742270335405 23.424689877144942 98-99 20.59853230131246 27.238729007675023 28.90522379148257 23.257514899529948 100-101 21.191044039081937 26.65719735510461 28.8379879476556 23.313770658157853 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 604.0 1 340.5 2 111.0 3 141.5 4 113.0 5 90.0 6 149.5 7 375.0 8 669.0 9 801.5 10 683.5 11 571.0 12 598.5 13 618.0 14 650.5 15 679.0 16 691.0 17 755.5 18 822.5 19 885.0 20 1028.5 21 1239.5 22 1441.5 23 1611.5 24 1880.0 25 2379.0 26 3095.0 27 3868.0 28 4421.0 29 4980.0 30 5733.5 31 6701.5 32 7899.0 33 8537.0 34 8759.0 35 9380.5 36 10338.5 37 11122.0 38 11285.5 39 11406.5 40 11685.5 41 12095.5 42 12333.0 43 12398.0 44 12657.0 45 12924.0 46 13007.5 47 13192.5 48 13367.0 49 13008.0 50 12310.5 51 11654.0 52 11115.0 53 10866.0 54 10750.0 55 9768.0 56 8499.5 57 7578.5 58 6834.0 59 5869.5 60 4714.0 61 3944.0 62 3253.0 63 2329.0 64 1576.5 65 1170.0 66 846.0 67 628.5 68 492.5 69 374.0 70 286.0 71 191.0 72 125.0 73 97.0 74 70.5 75 42.5 76 28.0 77 22.0 78 20.5 79 12.0 80 8.0 81 9.0 82 6.5 83 3.0 84 1.5 85 1.0 86 1.5 87 2.0 88 1.5 89 1.0 90 1.0 91 0.5 92 0.0 93 1.0 94 1.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3327638351505831 2 0.281048706986416 3 0.27915338815317425 4 0.2815902266530565 5 0.281048706986416 6 0.2423300508216207 7 0.24530840898814343 8 0.24882828682130662 9 0.26047095965407724 10-11 0.26751071532040366 12-13 0.2551911429043324 14-15 0.2455791688214637 16-17 0.24219467090496058 18-19 0.22567832107242553 20-21 0.2347487754886538 22-23 0.22743825998900713 24-25 0.23204117715545136 26-27 0.2279797796556476 28-29 0.2473391077380453 30-31 0.2629077981539594 32-33 0.2551911429043324 34-35 0.24882828682130662 36-37 0.23556105498861454 38-39 0.21999236457270038 40-41 0.21701400640617763 42-43 0.23204117715545136 44-45 0.2300104784055495 46-47 0.24314233032158147 48-49 0.25275430440445024 50-51 0.2656153964871619 52-53 0.2818609864863767 54-55 0.2505882257378882 56-57 0.218503185489439 58-59 0.20455905407344632 60-61 0.20699589257332854 62-63 0.21417102815631506 64-65 0.20496519382342668 66-67 0.21065115032315188 68-69 0.20929735115655063 70-71 0.21051577040649172 72-73 0.21417102815631506 74-75 0.22269996290590283 76-77 0.2346133955719937 78-79 0.23501953532197406 80-81 0.22621984073906604 82-83 0.21430640807297519 84-85 0.2026637352402046 86-87 0.20537133357340706 88-89 0.21918008507273964 90-91 0.21647248673953715 92-93 0.22161692357262186 94-95 0.2155248273229163 96-97 0.22960433865556912 98-99 0.2336657361553728 100-101 0.2090265913232304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 369331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.51382787379977 #Duplication Level Percentage of deduplicated Percentage of total 1 88.38955256785856 64.9785435331573 2 7.2509514949920435 10.660904002482324 3 1.8736201084598119 4.132109584626139 4 0.7670794840169679 2.2556379661418613 5 0.44318729575141996 1.629019728786234 6 0.27081749859486975 1.1945298586149762 7 0.18828274342223836 0.9688969634084466 8 0.14413604480281028 0.8476793910435271 9 0.10246672782326051 0.6779449252791597 >10 0.5198650301936213 6.839133027742577 >50 0.027228193400927605 1.354899790959131 >100 0.021341016445870314 2.770931015533085 >500 3.6794855941155717E-4 0.17014014142623102 >1k 0.0011038456782346713 1.5196300707989918 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2371 0.6419715648023047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1946 0.5268986356411999 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1301 0.35225854314964083 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 629 0.17030793515843512 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 455 0.1231957241607122 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 371 0.10045189816181149 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.707598333202466E-4 14-15 0.0 0.0 0.0 0.0 2.707598333202466E-4 16-17 0.0 0.0 0.0 2.707598333202466E-4 2.707598333202466E-4 18-19 0.0 0.0 0.0 2.707598333202466E-4 2.707598333202466E-4 20-21 0.0 0.0 0.0 2.707598333202466E-4 2.707598333202466E-4 22-23 0.0 0.0 0.0 5.415196666404932E-4 2.707598333202466E-4 24-25 0.0 0.0 0.0 8.122794999607399E-4 2.707598333202466E-4 26-27 0.0 0.0 0.0 0.001353799166601233 2.707598333202466E-4 28-29 0.0 0.0 0.0 0.0035198778331632058 2.707598333202466E-4 30-31 0.0 0.0 0.0 0.007987415082947275 2.707598333202466E-4 32-33 0.0 0.0 0.0 0.018547048582436894 2.707598333202466E-4 34-35 0.0 0.0 0.0 0.033303459498390336 2.707598333202466E-4 36-37 0.0 0.0 0.0 0.05293354741410821 2.707598333202466E-4 38-39 0.0 0.0 0.0 0.0854247274125378 2.707598333202466E-4 40-41 0.0 0.0 0.0 0.12658022207721528 2.707598333202466E-4 42-43 0.0 0.0 0.0 0.17924300965800324 2.707598333202466E-4 44-45 0.0 0.0 0.0 0.248151387238006 2.707598333202466E-4 46-47 0.0 0.0 0.0 0.3269424987341978 2.707598333202466E-4 48-49 0.0 0.0 0.0 0.4296958554792314 2.707598333202466E-4 50-51 0.0 0.0 0.0 0.556682217306427 2.707598333202466E-4 52-53 0.0 0.0 0.0 0.698289610132916 2.707598333202466E-4 54-55 0.0 0.0 0.0 0.8386785837094639 2.707598333202466E-4 56-57 0.0 0.0 0.0 1.034302563283342 2.707598333202466E-4 58-59 0.0 0.0 0.0 1.250639670106219 2.707598333202466E-4 60-61 0.0 0.0 0.0 1.4978433979276042 2.707598333202466E-4 62-63 0.0 0.0 0.0 1.7997406120796793 2.707598333202466E-4 64-65 0.0 0.0 0.0 2.0902659132323036 2.707598333202466E-4 66-67 0.0 0.0 0.0 2.4564685877979375 2.707598333202466E-4 68-69 0.0 0.0 0.0 2.902003893526403 2.707598333202466E-4 70-71 0.0 0.0 0.0 3.4391914028337727 2.707598333202466E-4 72-73 0.0 0.0 0.0 4.022272703888923 2.707598333202466E-4 74-75 0.0 0.0 0.0 4.599803428361009 2.707598333202466E-4 76-77 0.0 0.0 0.0 5.261675840912352 2.707598333202466E-4 78-79 0.0 0.0 0.0 6.048097776791009 2.707598333202466E-4 80-81 0.0 0.0 0.0 6.987634398412265 2.707598333202466E-4 82-83 0.0 0.0 0.0 7.880600328702437 2.707598333202466E-4 84-85 0.0 0.0 0.0 8.867655301071396 2.707598333202466E-4 86-87 0.0 0.0 0.0 9.921317192437137 2.707598333202466E-4 88-89 0.0 0.0 0.0 11.118752555295927 2.707598333202466E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGTCG 25 0.0050092908 57.02666 5 GGTATCA 940 0.0 46.530075 1 GTATCAA 1795 0.0 42.641766 1 TATCAAC 2305 0.0 33.399563 2 ATCAACG 2320 0.0 33.183617 3 TCAACGC 2320 0.0 33.183617 4 CAACGCA 2345 0.0 33.0325 5 AACGCAG 2420 0.0 32.004417 6 CGCAGAG 2675 0.0 28.953527 8 ACGCAGA 2720 0.0 28.29983 7 GCAGAGT 2865 0.0 26.038305 9 GTACTGA 135 1.3360654E-5 24.6378 6 TACATGG 1535 0.0 23.219324 2 GTACATG 1610 0.0 23.032576 1 GCCTTAT 145 2.3130597E-5 22.951117 1 CCCTATA 145 2.3203651E-5 22.941761 2 GTGTAGC 230 3.5706762E-9 22.737288 1 TAAGACT 190 3.2596472E-7 22.510525 4 AGTACTT 1855 0.0 22.40092 12-13 GAGTACT 1770 0.0 22.269299 12-13 >>END_MODULE