FastQCFastQC Report
Thu 26 May 2016
SRR937167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369331
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22540.6102926643038359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19730.5342091511408465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.37391932981526055No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC7280.19711315865713952No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA6580.17815997032472225No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA5700.15433310499254055No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.15243778615929882No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA4590.1242787634939932No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA4570.12373724382735271No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12250.044.99371
GTATCAA20550.036.300921
GTGTAAG2400.029.6968121
ATCAACG25400.029.1705273
TATCAAC25650.029.071382
TCAACGC25500.029.0561314
ACCGAGT1153.8498874E-628.9181948
CAACGCA26050.028.4426635
AACGCAG26500.028.13896
GTATAGG1554.555841E-827.5892941
TATAGGG1554.573849E-827.578092
TGTCGGA350.00839068527.1421866-67
TAAGGTG2409.094947E-1225.7267845
TAAGGTA1501.057706E-625.3309864
CGCAGAG29500.025.1231218
ACGCAGA29600.025.0382467
TGTAAGG2301.3460522E-1024.7803122
GTACAGG5200.024.6711961
GTCTAGA1751.4759644E-724.4362341
GTAAGGC1452.3304656E-522.928913
Produced by FastQC (version 0.11.5)